CN104066745B - 溶菌酶作为标签的用途 - Google Patents

溶菌酶作为标签的用途 Download PDF

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Publication number
CN104066745B
CN104066745B CN201380006389.4A CN201380006389A CN104066745B CN 104066745 B CN104066745 B CN 104066745B CN 201380006389 A CN201380006389 A CN 201380006389A CN 104066745 B CN104066745 B CN 104066745B
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CN
China
Prior art keywords
lysozyme
protein
polypeptide
albumen
fusion protein
Prior art date
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Expired - Fee Related
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CN201380006389.4A
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English (en)
Chinese (zh)
Other versions
CN104066745A (zh
Inventor
S·黑特尔
S·耶格
D·道贝特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Morphosys GmbH
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Morphosys GmbH
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Publication of CN104066745A publication Critical patent/CN104066745A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201380006389.4A 2012-01-23 2013-01-23 溶菌酶作为标签的用途 Expired - Fee Related CN104066745B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261589408P 2012-01-23 2012-01-23
US61/589,408 2012-01-23
EP12152095.1 2012-01-23
EP12152095 2012-01-23
PCT/EP2013/051181 WO2013110627A1 (en) 2012-01-23 2013-01-23 Use of lysozyme as a tag

Publications (2)

Publication Number Publication Date
CN104066745A CN104066745A (zh) 2014-09-24
CN104066745B true CN104066745B (zh) 2017-04-12

Family

ID=48872896

Family Applications (1)

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CN201380006389.4A Expired - Fee Related CN104066745B (zh) 2012-01-23 2013-01-23 溶菌酶作为标签的用途

Country Status (10)

Country Link
US (1) US9689014B2 (https=)
EP (1) EP2807179B1 (https=)
JP (1) JP6215231B2 (https=)
KR (1) KR20140122729A (https=)
CN (1) CN104066745B (https=)
AU (1) AU2013211651C1 (https=)
CA (1) CA2860916A1 (https=)
ES (1) ES2582802T3 (https=)
IN (1) IN2014DN06660A (https=)
WO (1) WO2013110627A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105524942A (zh) * 2016-02-05 2016-04-27 广州赛莱拉干细胞科技股份有限公司 一种表达载体及胰岛素样生长因子结合蛋白3的表达方法
US10986850B2 (en) 2016-12-21 2021-04-27 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
WO2018113745A1 (en) * 2016-12-21 2018-06-28 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
CN107794254A (zh) * 2017-09-30 2018-03-13 昆明理工大学 重组高温裂解酶的快速纯化方法
WO2022133068A1 (en) * 2020-12-16 2022-06-23 Danisco Us Inc Enzymes and enzyme compositions for cleaning
CN118974227A (zh) * 2022-03-01 2024-11-15 丹尼斯科美国公司 用于清洁的酶和酶组合物
WO2025179145A1 (en) * 2024-02-23 2025-08-28 Dualitas Therapeutics, Inc. Bispecific screening compositions and methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0332323A1 (en) * 1988-03-10 1989-09-13 Chisso Corporation Carrier for affinity chromatography immobilized with antibodies and preparation thereof
WO2001000855A1 (en) * 1999-06-23 2001-01-04 Ppl Therapeutics (Scotland) Ltd. Fusion proteins incorporating lysozyme

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2459701A (en) 1945-07-16 1949-01-18 Harper Esther Pardee Mechanical instrument holder
US4355022A (en) 1981-07-01 1982-10-19 Interon, Inc. Method of dental treatment
DE69024323T2 (de) 1989-10-27 1996-10-17 Genencor Int Antimikrobielles Verfahren und Formulierung unter Verwendung von Endoglycosidase vom Typ II und antimikrobielles Mittel
EP1007842B1 (de) 1997-08-22 2004-12-15 Siemens Aktiengesellschaft Verfahren zur klopfregelung einer brennkraftmaschine
PL347250A1 (en) 1998-10-15 2002-03-25 Dsm Nv Antimicrobial enzymes in animal feed
CA2472477C (en) 2002-01-07 2012-03-20 Lifesensors, Inc. Methods and compositions for protein expression and purification
DE10237317B4 (de) 2002-08-15 2010-04-08 3M Espe Ag Enzymhaltige Zusammensetzung, Verfahren zu deren Herstellung und deren Verwendung
CA2404356A1 (en) 2002-09-18 2004-03-18 Canadian Inovatech Inc. Gram-positive antibacterial composition and method for use
US7428273B2 (en) 2003-09-18 2008-09-23 Promptu Systems Corporation Method and apparatus for efficient preamble detection in digital data receivers
JP4891220B2 (ja) 2004-02-25 2012-03-07 ノボザイムス アクティーゼルスカブ 菌類細胞壁分解酵素
PL390235A1 (pl) 2007-04-10 2010-07-05 Saint Simeon Ldasaint Simeon Lda Nowe kompozycje zawierające lizozym i polisacharydy C-1/C-4 i ich zastosowanie do pielęgnacji jamy ustnej, w kosmetologii i dermatologii, antykoncepcji, urologii i ginekologii
AU2008311869B2 (en) 2007-10-17 2013-11-21 The Board Of Trustees Of The Leland Stanford Junior University Method and composition for crystallizing G protein-coupled receptors
CN101817883B (zh) * 2010-03-12 2012-06-13 广东药学院 一种家蝇天蚕素-人溶菌酶融合蛋白及其制备方法和应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0332323A1 (en) * 1988-03-10 1989-09-13 Chisso Corporation Carrier for affinity chromatography immobilized with antibodies and preparation thereof
WO2001000855A1 (en) * 1999-06-23 2001-01-04 Ppl Therapeutics (Scotland) Ltd. Fusion proteins incorporating lysozyme

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
A Three-dimensional Model of an Anti-lysozyme Antibody;Sandra J.Smith-Gill et al.;《J.Mol.Biol.》;19870420;第194卷(第4期);713-724 *

Also Published As

Publication number Publication date
JP2015504683A (ja) 2015-02-16
CN104066745A (zh) 2014-09-24
AU2013211651B2 (en) 2017-03-02
WO2013110627A1 (en) 2013-08-01
US9689014B2 (en) 2017-06-27
EP2807179A1 (en) 2014-12-03
AU2013211651C1 (en) 2017-10-12
EP2807179B1 (en) 2016-04-20
JP6215231B2 (ja) 2017-10-18
CA2860916A1 (en) 2013-08-01
US20140349343A1 (en) 2014-11-27
AU2013211651A1 (en) 2014-07-24
HK1202298A1 (en) 2015-09-25
ES2582802T3 (es) 2016-09-15
IN2014DN06660A (https=) 2015-05-22
KR20140122729A (ko) 2014-10-20

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