KR100729971B1 - 만능 줄기 세포에서 유래되는 간세포 계통 세포 - Google Patents
만능 줄기 세포에서 유래되는 간세포 계통 세포 Download PDFInfo
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Abstract
Description
Claims (34)
- 세포군 내의 60% 이상의 세포가 영장류 만능 줄기 (primate pluripotent stem, pPS) 세포주에서 분화된 것인 특징을 갖고 하기 목록으로부터 3 가지 이상의 추가적인 특징을 갖는, 시험관 내에서 배양될 수 있는 분화된 세포군:ㆍ항체로 검출가능한 α1-안티트립신 (AAT) 의 발현;ㆍ항체로 검출가능한 알부민의 발현;ㆍ항체로 검출가능한 α-페토단백질 (fetoprotein) 발현의 부재;ㆍRT-PCR 로 검출가능한 아시알로당단백질 수용체 (ASGR) 의 발현;ㆍ글리코겐 저장의 증거;ㆍ시토크롬 p450 활성의 증거;ㆍ글루코오스-6-포스파타아제 활성의 증거; 및ㆍ간세포의 형태학적 특색.
- 영장류 만능 줄기 (pPS) 세포주; 및 상기와 동일한 pPS 세포주에서 분화된 것을 특징으로 하고 하기 목록으로부터 3 가지 이상의 추가적인 특징을 갖는 분화된 세포군으로 이루어진 2 가지 단리된 세포군 세트:ㆍ항체로 검출가능한 α1-안티트립신 (AAT) 의 발현;ㆍ항체로 검출가능한 알부민의 발현;ㆍ항체로 검출가능한 α-페토단백질 (fetoprotein) 발현의 부재;ㆍRT-PCR 로 검출가능한 아시알로당단백질 수용체 (ASGR) 의 발현;ㆍ글리코겐 저장의 증거;ㆍ시토크롬 p450 활성의 증거;ㆍ글루코오스-6-포스파타아제 활성의 증거; 및ㆍ간세포의 형태학적 특색.
- 제 1 항 또는 제 2 항에 있어서, 상기 pPS 세포가 인간 배아 줄기 (hES) 세포인 세포군(들).
- 하기를 포함하는, 시험관 내에서 배양될 수 있는 분화된 세포를 수득하기 위한 영장류 만능 줄기 (pPS) 세포의 처리 방법:a) 인간 만능 줄기 세포의 배양물을 제공;b) 분화된 세포를 농축시키는 조건 하에서 간세포 분화 제제를 함유하는 배양 배지 중의 기질 상에서 상기 세포를 배양.
- 제 4 항에 있어서, 하기 특징 중 1 가지 이상을 가지는 방법:ㆍ세포를 배양하여 배상체를 형성하는 단계; 상기 배상체를 세포외 매트릭스 상에 플레이팅하는 단계; 간세포 분화 제제를 상기 배상체의 플레이팅과 동시에 또는 그 후에 상기 배지에 첨가하는 단계; 및 그 후 분화된 세포를 농축시키는 조건 하에서 상기 플레이팅된 세포를 간세포 분화 제제와 함께 배양하는 단계에 의하여 pPS 세포 배양물을 제조함;ㆍ상기 간세포 분화 제제를, 세포외 매트릭스는 함유하나 영양 세포는 본질적으로 없는 pPS 세포의 배양물에 첨가하고; 그 후 분화된 세포를 농축시키는 조건 하에서 상기 pPS 세포를 상기 간세포 분화 제제와 함께 배양함;ㆍ상기 간세포 분화 제제는 n-부티레이트와 같은 히스톤 디아세틸라아제 억제제임;ㆍ상기 세포를, 상기 간세포 분화 제제와의 배양과 동시적으로 또는 순차적으로 1 가지 이상의 간세포 성숙 인자와 함께 배양하는 것으로서, 이때 상기 간세포 성숙 인자 중 하나 이상이 디메틸 술폭시드 (DMSO), 디메틸아세트아미드 (DMA); 헥사메틸렌 비스아세트아미드 또는 기타 폴리메틸렌 비스아세트아미드와 같은 유기 용매, 또는, 글루코코르티코이드, 표피 성장 인자 (EGF), 인슐린, TGF-α, TGF-β, 섬유아세포 성장 인자 (FGF), 헤파린, 간세포 성장 인자 (HGF), IL-1, IL-6, IGF-I, IGF-II 또는 HBGF-1 과 같은 시토카인 또는 호르몬 중 하나임.
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- 제 4 항 또는 제 5 항에 있어서, 상기 pPS 세포가 인간 배아 줄기 (hES) 세포인 방법.
- 제 1 항 또는 제 2 항에 따른, 또는 제 4 항 또는 제 5 항의 방법에 따라 제조된 분화된 세포를 화합물과 배합하고 상기 화합물이 세포에 독성인지 여부를 결정하는 것을 포함하는, 화합물의 간세포 독성 스크리닝 방법.
- 제 1 항 또는 제 2 항에 따른, 또는 제 4 항 또는 제 5 항의 방법에 따라 제조된 분화된 세포를 화합물과 배합하고, 상기 화합물과의 접촉에 따른 세포 내 임의의 표현형 또는 대사적 변화를 측정하여 상기 변화를 간세포 기능의 조절능과 연관시키는 것을 포함하는, 화합물의 간세포 독성 스크리닝 방법.
- 세포가 플루이드 중의 독소를 제거하거나 변형시킬 수 있게 하는 조건 하에서 제 1 항 또는 제 2 항에 따른, 또는 제 4 항 또는 제 5 항의 방법에 따라 제조된 분화된 세포를 상기 플루이드와 접촉시키는 것을 포함하는, 생체 밖에서의 플루이드의 해독 방법.
- 제 1 항 또는 제 2 항에 따른, 또는 제 4 항 또는 제 5 항의 방법에 따라 제조된 분화된 세포를 포함하는, 개인에서의 간세포 기능의 복원 또는 보충용 약학 제제.
- 제 1 항 또는 제 2 항에 따른, 또는 제 4 항 또는 제 5 항의 방법에 따라 제조된 분화된 세포가, 환자로부터 유입되고 장치에 의해 해독된 후 상기 환자에게 복귀되는 혈액을 해독시키도록 위치되는 간 보조 장치.
- 제 5 항에 있어서, 세포외 매트릭스가 라미닌, 콜라겐 또는 엥겔브레드-홀름-스왐 (Engelbreth-Holm-Swarm) 세포의 매트릭스인 방법.
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Application Number | Priority Date | Filing Date | Title |
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US20009500P | 2000-04-27 | 2000-04-27 | |
US60/200,095 | 2000-04-27 | ||
US09/718,308 | 2000-11-20 | ||
US09/718,308 US6458589B1 (en) | 2000-04-27 | 2000-11-20 | Hepatocyte lineage cells derived from pluripotent stem cells |
PCT/US2001/013471 WO2001081549A2 (en) | 2000-04-27 | 2001-04-26 | Hepatocyte lineage cells derived from pluripotent stem cells |
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US (2) | US6458589B1 (ko) |
EP (2) | EP1280887A2 (ko) |
JP (2) | JP5711441B2 (ko) |
KR (1) | KR100729971B1 (ko) |
CN (2) | CN102031241B (ko) |
AU (3) | AU2001259170B2 (ko) |
CA (1) | CA2407505C (ko) |
GB (1) | GB2380490B (ko) |
IL (2) | IL152481A0 (ko) |
WO (1) | WO2001081549A2 (ko) |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6822267B1 (en) * | 1997-08-20 | 2004-11-23 | Advantest Corporation | Signal transmission circuit, CMOS semiconductor device, and circuit board |
US7410798B2 (en) | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
US20050042749A1 (en) * | 2001-05-16 | 2005-02-24 | Carpenter Melissa K. | Dopaminergic neurons and proliferation-competent precursor cells for treating Parkinson's disease |
US7473555B2 (en) * | 2000-04-27 | 2009-01-06 | Geron Corporation | Protocols for making hepatocytes from embryonic stem cells |
US6458589B1 (en) | 2000-04-27 | 2002-10-01 | Geron Corporation | Hepatocyte lineage cells derived from pluripotent stem cells |
JP5943533B2 (ja) | 2000-05-17 | 2016-07-06 | アステリアス バイオセラピューティクス インコーポレイテッド | 神経前駆細胞の集団 |
US7250294B2 (en) * | 2000-05-17 | 2007-07-31 | Geron Corporation | Screening small molecule drugs using neural cells differentiated from human embryonic stem cells |
AU2001268449A1 (en) * | 2000-06-14 | 2001-12-24 | Vistagen, Inc. | Toxicity typing using liver stem cells |
WO2002026942A2 (en) * | 2000-09-25 | 2002-04-04 | Walter Reed Army Institute Of Research | Human liver cell line |
US7781186B2 (en) * | 2000-09-25 | 2010-08-24 | The United States Of America As Represented By The Secretary Of The Army | Screening methods using normal human liver cell line |
AU2002241758A1 (en) * | 2000-10-26 | 2002-06-18 | University Of Florida | Drug screening system |
US6576464B2 (en) * | 2000-11-27 | 2003-06-10 | Geron Corporation | Methods for providing differentiated stem cells |
US6921665B2 (en) * | 2000-11-27 | 2005-07-26 | Roslin Institute (Edinburgh) | Selective antibody targeting of undifferentiated stem cells |
EP1366148A2 (en) * | 2001-01-24 | 2003-12-03 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE DEPARTMENT OF HEALTH & HUMAN SERVICES | Differentiation of stem cells to pancreatic endocrine cells |
US20030211605A1 (en) * | 2001-05-01 | 2003-11-13 | Lee Sang-Hun | Derivation of midbrain dopaminergic neurons from embryonic stem cells |
WO2003017763A1 (en) * | 2001-08-24 | 2003-03-06 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Use of a histone deacetylase inhibitor to increase the entry of an adenoviral agent into a cell |
US7588937B2 (en) | 2001-10-03 | 2009-09-15 | Wisconsin Alumni Research Foundation | Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells |
US6887706B2 (en) | 2001-10-03 | 2005-05-03 | Wisconsin Alumni Research Foundation | Method of in vitro differentiation of transplantable neural precursor cells from primate embryonic stem cells |
US8153424B2 (en) | 2001-10-03 | 2012-04-10 | Wisconsin Alumni Research Foundation | Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells |
JPWO2003038076A1 (ja) * | 2001-10-31 | 2005-02-24 | 株式会社レノメディクス研究所 | 不死化間葉系細胞及びその利用 |
US20050095708A1 (en) * | 2001-11-09 | 2005-05-05 | Pera Martin F. | Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom |
KR101089591B1 (ko) | 2001-12-07 | 2011-12-05 | 제론 코포레이션 | 인간 배아 줄기세포 유래의 섬세포 |
WO2003050251A2 (en) | 2001-12-07 | 2003-06-19 | Geron Corporation | Hematopoietic cells from human embryonic stem cells |
JP4408371B2 (ja) | 2001-12-21 | 2010-02-03 | トロンボジェニクス・ナムローゼ・フエンノートシャップ | 生殖細胞系伝達能力を有する胚性幹(es)細胞のインビトロの誘導および培養のための組成物 |
US20050170506A1 (en) * | 2002-01-16 | 2005-08-04 | Primegen Biotech Llc | Therapeutic reprogramming, hybrid stem cells and maturation |
US20030134422A1 (en) * | 2002-01-16 | 2003-07-17 | Sayre Chauncey Bigelow | Stem cell maturation for all tissue lines |
US20050090004A1 (en) * | 2003-01-16 | 2005-04-28 | Sayre Chauncey B. | Stem cell maturation for all tissue lines |
WO2003069972A2 (en) * | 2002-02-15 | 2003-08-28 | Massachusetts Institute Of Technology | Hepatocyte precursor cell lines |
AU2003224681A1 (en) * | 2002-03-13 | 2003-09-29 | Bresagen Inc. | Compositions and methods for primate neural cell production |
WO2003080806A2 (en) * | 2002-03-18 | 2003-10-02 | National Jewish Medical And Research Center | Method for production of neutrophils and uses therefor |
WO2003080649A2 (en) * | 2002-03-20 | 2003-10-02 | Regents Of The University Of Minnesota | Induction of hepatocyte proliferation in vitro by inhibition of cell cycle inhibitors |
US7247477B2 (en) | 2002-04-16 | 2007-07-24 | Technion Research & Development Foundation Ltd. | Methods for the in-vitro identification, isolation and differentiation of vasculogenic progenitor cells |
US7354763B2 (en) | 2002-04-16 | 2008-04-08 | Technion Research & Development Foundation Ltd. | Generating vascular smooth muscle cells in vitro from ES cells |
US20030207448A1 (en) * | 2002-05-06 | 2003-11-06 | Revera Gregory Henry | Methodologies for the creation of pluripotent or multipotent human stem cells without creating or destroying a human embryo |
GB0210741D0 (en) * | 2002-05-10 | 2002-06-19 | Medical Res Council | Methods of therapy |
US20050101014A1 (en) * | 2002-07-11 | 2005-05-12 | Keirstead Hans S. | Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury |
US7154002B1 (en) | 2002-10-08 | 2006-12-26 | Takeda San Diego, Inc. | Histone deacetylase inhibitors |
JP2006507011A (ja) * | 2002-10-18 | 2006-03-02 | ユニヴァーシティ オヴ フロリダ | 骨髄細胞の分化 |
GB0224415D0 (en) * | 2002-10-21 | 2002-11-27 | Medical Res Council | Compositions |
US7153650B2 (en) | 2003-03-13 | 2006-12-26 | Geron Corporation | Marker system for preparing and characterizing high-quality human embryonic stem cells |
WO2004082638A2 (en) | 2003-03-17 | 2004-09-30 | Syrrx, Inc. | Histone deacetylase inhibitors |
US20070003526A1 (en) * | 2003-03-25 | 2007-01-04 | Shinichiro Hayashi | Blood vessel-specific organogenesis from embryonic stem cells on three-dimensional matrigel layer |
WO2004087896A2 (en) * | 2003-03-31 | 2004-10-14 | Pfizer Products Inc. | Hepatocyte differentiation of stem cells |
US20050233448A1 (en) * | 2003-06-18 | 2005-10-20 | Oh Steve K W | Materials and methods to produce stem cells |
US20050032208A1 (en) * | 2003-06-18 | 2005-02-10 | Oh Steve Kah Weng | Materials and methods to produce stem cells |
EP1641912B1 (en) * | 2003-06-20 | 2016-04-27 | Axiogenesis Ag | Tissue modelling in embryonic stem (es) cell system |
US20050014217A1 (en) * | 2003-07-18 | 2005-01-20 | Cytokinetics, Inc. | Predicting hepatotoxicity using cell based assays |
US20050042595A1 (en) * | 2003-08-14 | 2005-02-24 | Martin Haas | Banking of multipotent amniotic fetal stem cells |
US7332336B2 (en) * | 2003-08-19 | 2008-02-19 | Effector Cell Institute, Inc. | Methods for inducing differentiation of pluripotent cells |
JP2005095138A (ja) * | 2003-08-19 | 2005-04-14 | Effector Cell Institute Inc | 多分化能を有する細胞の分化誘導方法 |
WO2005033294A2 (en) * | 2003-09-30 | 2005-04-14 | Regents Of The University Of California | Methods for maintaining hepatocytes in culture and for differentiating embryonic stem cells along a hepatocyte lineage |
TWI280280B (en) * | 2003-12-09 | 2007-05-01 | Ind Tech Res Inst | Culture system and method for expansion and undifferentiated growth of human embryonic stem cells |
US20050159470A1 (en) * | 2003-12-19 | 2005-07-21 | Syrrx, Inc. | Histone deacetylase inhibitors |
US20050137234A1 (en) * | 2003-12-19 | 2005-06-23 | Syrrx, Inc. | Histone deacetylase inhibitors |
US7625753B2 (en) * | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
US8647873B2 (en) | 2004-04-27 | 2014-02-11 | Viacyte, Inc. | PDX1 expressing endoderm |
US7985585B2 (en) | 2004-07-09 | 2011-07-26 | Viacyte, Inc. | Preprimitive streak and mesendoderm cells |
US7541185B2 (en) * | 2003-12-23 | 2009-06-02 | Cythera, Inc. | Methods for identifying factors for differentiating definitive endoderm |
US8586357B2 (en) * | 2003-12-23 | 2013-11-19 | Viacyte, Inc. | Markers of definitive endoderm |
US20050266554A1 (en) * | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
EA200601231A1 (ru) * | 2003-12-23 | 2007-06-29 | Китера, Инк. | Дефинитивная энтодерма |
US7794704B2 (en) | 2004-01-23 | 2010-09-14 | Advanced Cell Technology, Inc. | Methods for producing enriched populations of human retinal pigment epithelium cells for treatment of retinal degeneration |
SG10201606441SA (en) | 2004-01-23 | 2016-09-29 | Astellas Inst For Regenerative Medicine | Improved modalities for the treatment of degenerative diseases of the retina |
WO2005097980A2 (en) * | 2004-03-26 | 2005-10-20 | Geron Corporation | New protocols for making hepatocytes from embryonic stem cells |
DK1740945T3 (en) | 2004-04-07 | 2019-01-21 | Ncardia Ag | KKE-INVASIVE, IN-VITRO FUNCTIONAL TISSUE TEST SYSTEMS |
US20050233449A1 (en) * | 2004-04-16 | 2005-10-20 | Kuan-Der Lee | Simple and rapid derivation of functional hepatocytes from human bone marrow-derived mesenchymal stem cells |
JP4814875B2 (ja) | 2004-05-11 | 2011-11-16 | アキシオジェネシス エージー | invitro分化細胞に基づく薬物発見のための検定 |
US20090010900A1 (en) * | 2004-07-07 | 2009-01-08 | The University Of North Carlolina At Chapel Hill | Embryonic Stem Cell Derivatives, and Methods of Making and Using the Same |
AU2005271944B2 (en) * | 2004-07-09 | 2012-02-16 | Viacyte, Inc. | Preprimitive streak and mesendoderm cells |
EP2267116B1 (en) | 2004-07-13 | 2017-05-31 | Asterias Biotherapeutics, Inc. | Growth medium for primate embryonic stem cells |
US20070196918A1 (en) * | 2004-07-15 | 2007-08-23 | Sayre Chauncey B | Reprogramming of adult human testicular stem cells to pluripotent germ-line stem cells |
US20070020759A1 (en) * | 2004-07-15 | 2007-01-25 | Primegen Biotech Llc | Therapeutic reprogramming of germ line stem cells |
WO2006020919A2 (en) * | 2004-08-13 | 2006-02-23 | University Of Georgia Research Foundation, Inc. | Compositions and methods for self-renewal and differentiation in human embryonic stem cells |
US20070298016A1 (en) * | 2004-09-29 | 2007-12-27 | Nico Heins | Methods For The Generation Of Hepatocyte-Like Cells From Human Blastocyst-Derived Stem (Hbs) |
EP1824965B1 (en) * | 2004-10-27 | 2011-10-05 | Vrije Universiteit Brussel | Hepatic differentiation of stem cells |
US7642275B2 (en) * | 2004-12-16 | 2010-01-05 | Takeda San Diego, Inc. | Histone deacetylase inhibitors |
US8017395B2 (en) | 2004-12-17 | 2011-09-13 | Lifescan, Inc. | Seeding cells on porous supports |
CA2512667A1 (en) * | 2005-01-07 | 2006-07-07 | Takahiro Ochiya | Human hepatocyte-like cells and uses thereof |
EP1842905A1 (en) * | 2005-02-03 | 2007-10-10 | National University Corporation Okayama University | Method of inducing differentiation of embryo-stem cell into hepatocyte and hepatocyte induced by the method |
WO2006122319A2 (en) * | 2005-05-11 | 2006-11-16 | Takeda San Diego, Inc. | Histone deacetylase inhibitors |
US20110311977A1 (en) * | 2005-05-17 | 2011-12-22 | Arundhati Mandal | In Vitro Generation of Hepatocytes from Human Embryonic Stem Cells |
AU2006202209B2 (en) * | 2005-05-27 | 2011-04-14 | Lifescan, Inc. | Amniotic fluid derived cells |
EP1728858A1 (en) | 2005-05-31 | 2006-12-06 | Abcys S. A. | Primitive endodermal stem cells, a process for preparing them and their use, in particular for obtaining primitive epithelial liver cells |
CN101484575B (zh) * | 2005-06-08 | 2013-10-02 | 森托科尔公司 | 用于眼变性的细胞疗法 |
US20060292694A1 (en) * | 2005-06-22 | 2006-12-28 | Roslin Institute | Reporter hepatocytes and other cells for drug screening and toxicity testing |
US20060292695A1 (en) * | 2005-06-22 | 2006-12-28 | Roslin Institute | Methods and kits for drug screening and toxicity testing using promoter-reporter cells derived from embryonic stem cells |
JP5560391B2 (ja) * | 2005-06-22 | 2014-07-23 | アステリアス バイオセラピューティクス インコーポレイテッド | ヒト胚性幹細胞の懸濁培養法 |
CA2613529A1 (en) * | 2005-06-22 | 2007-01-04 | Geron Corporation | Reporter hepatocytes and other cells for drug screening and toxicity testing |
ZA200800901B (en) * | 2005-07-14 | 2010-05-26 | Takeda San Diego Inc | Histone deacetylase inhibitors |
DK2674485T3 (da) | 2005-10-27 | 2019-08-26 | Viacyte Inc | Pdx-1 udtrykkende dorsal og ventral fortarm endoderm |
PL2535403T3 (pl) * | 2005-12-08 | 2019-10-31 | Univ Louisville Res Found Inc | Bardzo małe komórki macierzyste typu embrionalnego (VSEL) oraz sposoby ich izolowania i zastosowania |
US9155762B2 (en) * | 2005-12-08 | 2015-10-13 | University Of Louisville Research Foundation, Inc. | Uses and isolation of stem cells from bone marrow |
US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
US8129187B2 (en) | 2005-12-13 | 2012-03-06 | Kyoto University | Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 |
EP4223769A3 (en) | 2005-12-13 | 2023-11-01 | Kyoto University | Nuclear reprogramming factor |
JP2009525955A (ja) * | 2006-01-13 | 2009-07-16 | タケダ サン ディエゴ インコーポレイテッド | ヒストンデアセチラーゼ阻害剤 |
US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
US11254916B2 (en) | 2006-03-02 | 2022-02-22 | Viacyte, Inc. | Methods of making and using PDX1-positive pancreatic endoderm cells |
AU2007224116B2 (en) * | 2006-03-02 | 2013-11-07 | Viacyte, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
FR2898908A1 (fr) | 2006-03-24 | 2007-09-28 | Agronomique Inst Nat Rech | Procede de preparation de cellules aviaires differenciees et genes impliques dans le maintien de la pluripotence |
US8741643B2 (en) | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
WO2007143117A2 (en) * | 2006-06-02 | 2007-12-13 | Geron Corporation | Differentiation of primate pluripotent cells to hepatocyte-lineage cells |
WO2007149182A2 (en) | 2006-06-19 | 2007-12-27 | Geron Corporation | Differentiation and enrichment of islet-like cells from human pluripotent stem cells |
US20080003676A1 (en) * | 2006-06-26 | 2008-01-03 | Millipore Corporation | Growth of embryonic stem cells |
US20100323442A1 (en) * | 2006-10-17 | 2010-12-23 | Emmanuel Edward Baetge | Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells |
AR065584A1 (es) * | 2007-03-01 | 2009-06-17 | Cryo Cell Internat Inc | Obtencion aislamiento y crioconservacion de celulas endometriales / menstruales |
EP2634575A1 (en) * | 2007-03-16 | 2013-09-04 | Cellartis AB | A combined scalable in vitro differentiation system for human blastocyst-derived stem (hbs) cells or cells derived from hbs cells for direct assay application in multiwell plates |
US20100086525A1 (en) * | 2007-05-03 | 2010-04-08 | Zaret Kenneth S | Compositions and Methods for Altering Pancreas or Liver Function |
JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
US9213999B2 (en) | 2007-06-15 | 2015-12-15 | Kyoto University | Providing iPSCs to a customer |
JP2010532172A (ja) * | 2007-07-01 | 2010-10-07 | ライフスキャン・インコーポレイテッド | 単細胞の分化万能性幹細胞の培養 |
US9080145B2 (en) * | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
ES2648128T3 (es) | 2007-07-31 | 2017-12-28 | Lifescan, Inc. | Diferenciación de células madre pluripotentes usando células alimentadoras humanas |
CN101952415B (zh) | 2007-07-31 | 2017-06-27 | 生命扫描有限公司 | 人胚胎干细胞的分化 |
US7695963B2 (en) | 2007-09-24 | 2010-04-13 | Cythera, Inc. | Methods for increasing definitive endoderm production |
AU2008312007A1 (en) | 2007-10-12 | 2009-04-23 | Advanced Cell Technology, Inc. | Improved methods of producing RPE cells and compositions of RPE cells |
CN107574142B (zh) | 2007-11-27 | 2021-07-06 | 生命扫描有限公司 | 人胚胎干细胞的分化 |
US9683232B2 (en) | 2007-12-10 | 2017-06-20 | Kyoto University | Efficient method for nuclear reprogramming |
KR101532442B1 (ko) | 2007-12-10 | 2015-06-29 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 효율적인 핵 초기화 방법 |
SG188098A1 (en) | 2008-01-30 | 2013-03-28 | Geron Corp | Synthetic surfaces for culturing stem cell derived cardiomyocytes |
WO2009105570A2 (en) | 2008-02-21 | 2009-08-27 | Centocor Ortho Biotech Inc. | Methods, surface modified plates and compositions for cell attachment, cultivation and detachment |
US20100087002A1 (en) * | 2008-02-21 | 2010-04-08 | Benjamin Fryer | Methods, Surface Modified Plates and Compositions for Cell Attachment, Cultivation and Detachment |
US7939322B2 (en) | 2008-04-24 | 2011-05-10 | Centocor Ortho Biotech Inc. | Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm |
US8623648B2 (en) | 2008-04-24 | 2014-01-07 | Janssen Biotech, Inc. | Treatment of pluripotent cells |
EP2268809B1 (en) | 2008-05-02 | 2019-02-06 | Kyoto University | Method of nuclear reprogramming |
JP5734183B2 (ja) | 2008-06-30 | 2015-06-17 | ヤンセン バイオテツク,インコーポレーテツド | 多能性幹細胞の分化 |
US20100028307A1 (en) * | 2008-07-31 | 2010-02-04 | O'neil John J | Pluripotent stem cell differentiation |
EP2343361A4 (en) | 2008-10-24 | 2013-10-09 | Kuraray Co | CELL CULTURE KIT, SCREENING METHOD, AND METHOD FOR MANUFACTURING THE CELL CULTURE KIT |
BRPI0919885A2 (pt) * | 2008-10-31 | 2015-08-11 | Centocor Ortho Biotech Inc | Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática |
CA2742267C (en) * | 2008-10-31 | 2019-06-04 | Centocor Ortho Biotech Inc. | Differentiation of human embryonic stem cells to the pancreatic endocrine lineage |
CN105349517B (zh) | 2008-11-14 | 2021-05-04 | 维赛特公司 | 源于人多能干细胞的胰腺细胞的包封 |
RU2555538C2 (ru) | 2008-11-20 | 2015-07-10 | Сентокор Орто Байотек Инк. | Культура плюрипотентных стволовых клеток на микроносителях |
AU2009316583B2 (en) * | 2008-11-20 | 2016-04-21 | Janssen Biotech, Inc. | Methods and compositions for cell attachment and cultivation on planar substrates |
US20100143313A1 (en) * | 2008-12-10 | 2010-06-10 | The General Hospital Corporation | Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells |
CA2747398C (en) * | 2008-12-17 | 2023-06-20 | The Scripps Research Institute | Generation and maintenance of stem cells |
EP2253700A1 (en) | 2009-05-13 | 2010-11-24 | Helmholtz-Zentrum für Infektionsforschung GmbH | A method for producing test systems from donors suffering from adverse effects of medicaments and /or medical treatments, and uses of said systems |
US8323966B2 (en) * | 2009-06-25 | 2012-12-04 | Geron Corporation | Differentiated pluripotent stem cell progeny depleted of extraneous phenotypes |
RU2540021C2 (ru) * | 2009-07-20 | 2015-01-27 | Янссен Байотек, Инк. | Дифференцировка эмбриональных стволовых клеток человека |
GB2485113B (en) * | 2009-07-20 | 2016-12-28 | Janssen Biotech Inc | Differentiation of human embryonic stem cells into cells of the pancreatic endoderm lineage |
EP2456862A4 (en) | 2009-07-20 | 2013-02-27 | Janssen Biotech Inc | DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS |
PL2494035T3 (pl) * | 2009-10-29 | 2018-07-31 | Janssen Biotech, Inc. | Pluripotencjalne komórki macierzyste |
US10485829B2 (en) | 2009-11-17 | 2019-11-26 | Astellas Institute For Regenerative Medicine | Methods of producing human RPE cells and pharmaceutical preparations of human RPE cells |
RU2586506C2 (ru) * | 2009-12-23 | 2016-06-10 | Янссен Байотек, Инк. | Дифференцировка человеческих эмбриональных стволовых клеток |
CN102741395B (zh) | 2009-12-23 | 2016-03-16 | 詹森生物科技公司 | 人胚胎干细胞的分化 |
AU2011223900A1 (en) | 2010-03-01 | 2012-09-13 | Janssen Biotech, Inc. | Methods for purifying cells derived from pluripotent stem cells |
SG184204A1 (en) * | 2010-03-23 | 2012-10-30 | Kuraray Co | Culture method for causing differentiation of pluripotent mammalian cells |
MX351515B (es) | 2010-05-12 | 2017-10-17 | Janssen Biotech Inc | Diferenciacion de celulas madre embrionarias humanas. |
US20110312001A1 (en) | 2010-06-15 | 2011-12-22 | Emile Nuwaysir | Compendium of ready-built stem cell models for interrogation of biological response |
EP2601289B1 (en) | 2010-08-04 | 2017-07-12 | Cellular Dynamics International, Inc. | Reprogramming immortalized b cells |
US9528090B2 (en) | 2010-08-31 | 2016-12-27 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
CA2809305C (en) | 2010-08-31 | 2019-06-11 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
AU2011296381B2 (en) | 2010-08-31 | 2016-03-31 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
WO2012048170A2 (en) | 2010-10-06 | 2012-04-12 | Massachusetts Institute Of Technology | Humanized animals via tissue engineering and uses therefor |
WO2012048275A2 (en) | 2010-10-08 | 2012-04-12 | Caridianbct, Inc. | Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system |
AU2012236707B2 (en) | 2011-03-29 | 2017-07-20 | Asterias Biotherapeutics, Inc. | Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells |
AU2012355698B2 (en) | 2011-12-22 | 2018-11-29 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
CA2864301C (en) | 2012-02-21 | 2021-07-06 | Wisconsin Alumni Research Foundation | Method of deriving mature hepatocytes from human embryonic stem cells |
KR20140131999A (ko) | 2012-03-07 | 2014-11-14 | 얀센 바이오테크 인코포레이티드 | 만능 줄기 세포의 증폭 및 유지를 위한 한정 배지 |
CN108103006A (zh) | 2012-06-08 | 2018-06-01 | 詹森生物科技公司 | 人胚胎干细胞向胰腺内分泌细胞的分化 |
WO2014053709A1 (en) | 2012-10-05 | 2014-04-10 | Glykos Finland Oy | A method for culturing stem cells |
KR102084561B1 (ko) | 2012-12-31 | 2020-03-04 | 얀센 바이오테크 인코포레이티드 | 췌장 내분비 세포로의 분화를 위한 공기-액체 계면에서의 인간 배아 줄기세포의 배양 |
KR102036780B1 (ko) | 2012-12-31 | 2019-10-25 | 얀센 바이오테크 인코포레이티드 | Hb9 조절제를 사용하는 인간 배아 줄기세포의 췌장 내분비 세포로의 분화 |
US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
JP6529440B2 (ja) | 2012-12-31 | 2019-06-12 | ヤンセン バイオテツク,インコーポレーテツド | 膵内分泌細胞への分化のためのヒト多能性細胞の懸濁及びクラスタリング |
US20140242595A1 (en) | 2013-02-22 | 2014-08-28 | Cellular Dynamics International, Inc. | Hepatocyte production via forward programming by combined genetic and chemical engineering |
EP2967505B8 (en) | 2013-03-15 | 2020-03-11 | Amniotics AB | Apparatuses for amniotic fluid collection |
KR20160048029A (ko) | 2013-03-15 | 2016-05-03 | 마르쿠스 카레 토를레이프 라르손 | 제대혈 수집 및 세포의 분리를 위한 세포, 방법 및 장치 |
JP6633522B2 (ja) | 2013-11-16 | 2020-01-22 | テルモ ビーシーティー、インコーポレーテッド | バイオリアクターにおける細胞増殖 |
US9512406B2 (en) | 2013-12-20 | 2016-12-06 | The J. David Gladstone Institute, a testamentary trust established under the Will of J. David Gladstone | Generating hepatocytes |
SG11201605785XA (en) | 2014-01-14 | 2016-08-30 | Univ Colorado State Res Found | Stem cell-derived hepatocytes in co-culture and uses thereof |
KR20150103431A (ko) * | 2014-03-03 | 2015-09-11 | 한국과학기술원 | 인간 줄기세포 유래 간세포를 이용한 면역 간독성 스크리닝 방법 |
EP3119879B1 (en) * | 2014-03-19 | 2019-12-25 | INSERM (Institut National de la Santé et de la Recherche Médicale) | A method for inducing human cholangiocyte differentiation |
WO2015148704A1 (en) | 2014-03-25 | 2015-10-01 | Terumo Bct, Inc. | Passive replacement of media |
WO2015164228A1 (en) | 2014-04-21 | 2015-10-29 | Cellular Dynamics International, Inc. | Hepatocyte production via forward programming by combined genetic and chemical engineering |
CA2945393C (en) | 2014-04-24 | 2021-03-23 | Board Of Regents, The University Of Texas System | Application of induced pluripotent stem cells to generate adoptive cell therapy products |
CA2949056A1 (en) | 2014-05-16 | 2015-11-19 | Janssen Biotech, Inc. | Use of small molecules to enhance mafa expression in pancreatic endocrine cells |
JP6830059B2 (ja) | 2014-09-26 | 2021-02-17 | テルモ ビーシーティー、インコーポレーテッド | スケジュール化された細胞フィーディング |
MA41048A (fr) * | 2014-10-07 | 2017-08-15 | Qualyst Transp Solutions Llc | Utilité d'une protéine dans la prédiction d'effets in vivo |
US10711249B2 (en) | 2014-12-26 | 2020-07-14 | Kyoto University | Method for inducing hepatocytes |
WO2016183231A1 (en) | 2015-05-12 | 2016-11-17 | Baker Group, LLP | Method and system for a bioartificial organ |
WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
US11312940B2 (en) | 2015-08-31 | 2022-04-26 | University Of Louisville Research Foundation, Inc. | Progenitor cells and methods for preparing and using the same |
DK3347456T3 (da) * | 2015-09-08 | 2024-02-19 | Us Health | Fremgangsmåde til reproducerbar differentiering af retinale pigmentepitelceller af klinisk kvalitet |
US11338065B2 (en) | 2015-10-08 | 2022-05-24 | Massachusetts Institute Of Technology | In situ expansion of engineered devices for regeneration |
EP3423568A4 (en) | 2016-03-04 | 2019-11-13 | University Of Louisville Research Foundation, Inc. | METHOD AND COMPOSITIONS FOR EX-VIVO REPRODUCTION OF VERY SMALL EMBRYONAL STEM CELLS (VSELS) |
MA45479A (fr) | 2016-04-14 | 2019-02-20 | Janssen Biotech Inc | Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen |
WO2017205667A1 (en) | 2016-05-25 | 2017-11-30 | Terumo Bct, Inc. | Cell expansion |
US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
CN105936889B (zh) * | 2016-06-24 | 2019-10-18 | 肇庆大华农生物药品有限公司 | 一种ad293球形细胞团的培养方法 |
US11542473B2 (en) | 2016-10-21 | 2023-01-03 | Amniotics Ab | Methods and compositions for generating hematopoietic cells |
US11629332B2 (en) | 2017-03-31 | 2023-04-18 | Terumo Bct, Inc. | Cell expansion |
US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
CN108611315B (zh) * | 2018-03-14 | 2022-02-15 | 北京平安普德生物技术有限公司 | 诱导人胚胎干细胞定向分化为肝样组织的培养基及诱导方法和应用 |
EP4234019A3 (en) | 2019-10-18 | 2023-09-13 | Amniotics AB | Processes and apparatuses for obtaining amniotic mesenchymal stem cells from amniotic fluid and cells derived thereof |
WO2021150300A1 (en) | 2020-01-22 | 2021-07-29 | Massachusetts Institute Of Technology | Inducible tissue constructs and uses thereof |
CN113692282A (zh) * | 2020-06-14 | 2021-11-23 | 浙江大学 | 一种生物活性物质组合物、包含所述组合物的无血清培养基及其用途 |
WO2021254296A1 (zh) * | 2020-06-14 | 2021-12-23 | 浙江大学 | 一种生物活性物质组合物、包含所述组合物的无血清培养基及其用途 |
EP4188965A1 (en) | 2020-07-14 | 2023-06-07 | Massachusetts Institute of Technology | Synthetic heparin mimetics and uses thereof |
Family Cites Families (53)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2789118A (en) | 1956-03-30 | 1957-04-16 | American Cyanamid Co | 16-alpha oxy-belta1, 4-pregnadienes |
US3007923A (en) | 1959-01-22 | 1961-11-07 | Lab Francais Chimiotherapie | Process for the fluorination of 9beta, 11beta-epoxy and 5alpha, 6alpha-epoxy steroids |
US4491632A (en) | 1979-10-22 | 1985-01-01 | The Massachusetts General Hospital | Process for producing antibodies to hepatitis virus and cell lines therefor |
US4444887A (en) | 1979-12-10 | 1984-04-24 | Sloan-Kettering Institute | Process for making human antibody producing B-lymphocytes |
DE3167442D1 (en) | 1980-07-07 | 1985-01-10 | Nat Res Dev | Improvements in or relating to cell lines |
US5030105A (en) | 1985-06-06 | 1991-07-09 | Centro De Investigacion Y Estudios Avanzados Del Instituto Politecnico Nacional | Process for the long-term surviving culture of hepatocytes |
US5510254A (en) | 1986-04-18 | 1996-04-23 | Advanced Tissue Sciences, Inc. | Three dimensional cell and tissue culture system |
GB2197915B (en) | 1986-11-19 | 1990-11-14 | Rolls Royce Plc | Improvements in or relating to fluid bearings |
JP3771253B2 (ja) | 1988-09-02 | 2006-04-26 | ダイアックス コープ. | 新規な結合タンパク質の生成と選択 |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
WO1991015573A1 (en) * | 1990-04-03 | 1991-10-17 | Southwest Foundation For Biomedical Research | An immortalized primate hepatocyte cell line |
EP0455905B1 (en) | 1990-05-11 | 1998-06-17 | Microprobe Corporation | Dipsticks for nucleic acid hybridization assays and methods for covalently immobilizing oligonucleotides |
EP0532522B1 (en) | 1990-05-16 | 1996-04-10 | Baylor College Of Medicine | A permanent human hepatocyte cell line and its use in a liver assist device (lad) |
GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
WO1992008808A1 (en) | 1990-11-14 | 1992-05-29 | Siska Diagnostics, Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
EP1559778A1 (en) | 1991-08-07 | 2005-08-03 | Albert Einstein College Of Medicine Of Yeshiva University | Proliferation of hepatocyte precursors |
WO1994008598A1 (en) | 1992-10-09 | 1994-04-28 | Advanced Tissue Sciences, Inc. | Liver reserve cells |
CA2150262C (en) | 1992-12-04 | 2008-07-08 | Kaspar-Philipp Holliger | Multivalent and multispecific binding proteins, their manufacture and use |
US5532156A (en) | 1993-10-08 | 1996-07-02 | The United States Of America As Represented By The Secretary Of Agriculture | Hepatocyte cell line derived from the epiblast of pig blastocysts |
US6432711B1 (en) | 1993-11-03 | 2002-08-13 | Diacrin, Inc. | Embryonic stem cells capable of differentiating into desired cell lines |
US5731417A (en) | 1994-04-25 | 1998-03-24 | Becton, Dickinson And Company | Cell culture substrates and method of making |
US5935849A (en) | 1994-07-20 | 1999-08-10 | Cytotherapeutics, Inc. | Methods and compositions of growth control for cells encapsulated within bioartificial organs |
US5858747A (en) | 1994-07-20 | 1999-01-12 | Cytotherapeutics, Inc. | Control of cell growth in a bioartificial organ with extracellular matrix coated microcarriers |
US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
US5837234A (en) | 1995-06-07 | 1998-11-17 | Cytotherapeutics, Inc. | Bioartificial organ containing cells encapsulated in a permselective polyether suflfone membrane |
US5922837A (en) | 1995-09-20 | 1999-07-13 | Merck & Co., Inc. | Antiprotozoal cyclic tetrapeptides |
US6017760A (en) | 1995-10-10 | 2000-01-25 | Rhode Island Hospital | Isolation and culture of porcine hepatocytes |
US5869243A (en) | 1996-03-05 | 1999-02-09 | Rhode Island Hospital | Immortalized hepatocytes |
US5925659A (en) | 1996-05-07 | 1999-07-20 | Merck & Co., Inc. | Antibacterial agents |
WO1997047734A1 (en) | 1996-06-14 | 1997-12-18 | The Regents Of The University Of California | In vitro derivation and culture of primate pluripotent stem cells and therapeutic uses thereof |
WO1997047307A1 (en) | 1996-06-14 | 1997-12-18 | The Uab Research Foundation | Use of histone deacetylase inhibitors to activate transgene expression |
EP0827742A1 (en) | 1996-09-04 | 1998-03-11 | Vrije Universiteit Brussel | Use of histone deacetylase inhibitors for treating fribosis or cirrhosis |
DE69739497D1 (de) | 1996-10-01 | 2009-08-27 | Geron Corp | Menschlische Telomerase katalytische Untereinheit |
JP2001508302A (ja) | 1997-01-10 | 2001-06-26 | ライフ テクノロジーズ,インコーポレイテッド | 胚性幹細胞血清置換 |
US6331406B1 (en) | 1997-03-31 | 2001-12-18 | The John Hopkins University School Of Medicine | Human enbryonic germ cell and methods of use |
US6387673B1 (en) | 1997-05-01 | 2002-05-14 | The Salk Institute For Biological Studies | Compounds useful for the modulation of processes mediated by nuclear hormone receptors, methods for the identification and use of such compounds |
EP1010705A4 (en) | 1997-09-02 | 2004-09-15 | Sumitomo Pharma | NEW CYCLIC TETRAPEPTIDE DERIVATIVES AND THEIR MEDICAL USE |
AU729377B2 (en) | 1997-10-23 | 2001-02-01 | Asterias Biotherapeutics, Inc. | Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture |
AU1395999A (en) | 1997-11-10 | 1999-05-31 | Salk Institute For Biological Studies, The | Methods for the use of inhibitors of co-repressors for the treatment of neoplastic diseases |
WO1999027113A1 (en) | 1997-11-26 | 1999-06-03 | Geron Corporation | Mouse telomerase reverse transcriptase |
US6262116B1 (en) | 1998-01-23 | 2001-07-17 | Sloan-Kettering Institute For Cancer Research | Transcription therapy for cancers |
JPH11225752A (ja) * | 1998-02-16 | 1999-08-24 | Jms Co Ltd | 人工肝臓用肝細胞クローンおよび体外循環肝補助装置 |
JP4321907B2 (ja) * | 1998-04-28 | 2009-08-26 | 正義 難波 | 新規ヒト由来不死化肝細胞株 |
EP0953633A1 (en) | 1998-04-28 | 1999-11-03 | Livercell L.L.C. | Cell culturing method and medium for producing proliferated, normal, differentiated human liver cells |
US6129911A (en) * | 1998-07-10 | 2000-10-10 | Rhode Island Hospital, A Lifespan Partner | Liver stem cell |
AU6162199A (en) | 1998-09-25 | 2000-04-17 | Harvard-Mit Division Of Health Science And Technology | Reversibly immortalized hepatocytes and methods of use |
AU6218899A (en) | 1998-10-12 | 2000-05-01 | Geron Bio-Med Limited | Porcine oocytes with improved developmental competence |
CN1351656B (zh) | 1999-01-19 | 2012-02-29 | 查珀尔希尔北卡罗来纳大学 | 人肝脏祖先 |
EP1154780A2 (en) * | 1999-02-26 | 2001-11-21 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Bone marrow transplantation for hepatic regeneration and repair |
US6866843B2 (en) * | 1999-12-06 | 2005-03-15 | Viacell, Inc. | Method of transplanting in a mammal and treating diabetes mellitus by administering a pseudo-islet like aggregate differentiated from a nestin-positive pancreatic stem cell |
AU2295201A (en) * | 1999-12-30 | 2001-07-16 | University Of Massachusetts | Spore-like cells and uses thereof |
JP2003523767A (ja) * | 2000-02-26 | 2003-08-12 | アーテイセル・サイエンスイーズ・インコーポレイテツド | 脂肪組織由来の間質細胞から生成した多能性幹細胞およびその用途 |
US6458589B1 (en) * | 2000-04-27 | 2002-10-01 | Geron Corporation | Hepatocyte lineage cells derived from pluripotent stem cells |
-
2000
- 2000-11-20 US US09/718,308 patent/US6458589B1/en not_active Expired - Lifetime
-
2001
- 2001-04-26 CA CA002407505A patent/CA2407505C/en not_active Expired - Fee Related
- 2001-04-26 IL IL15248101A patent/IL152481A0/xx unknown
- 2001-04-26 AU AU2001259170A patent/AU2001259170B2/en not_active Ceased
- 2001-04-26 JP JP2001578620A patent/JP5711441B2/ja not_active Expired - Fee Related
- 2001-04-26 CN CN201010528128.7A patent/CN102031241B/zh not_active Expired - Fee Related
- 2001-04-26 EP EP01932661A patent/EP1280887A2/en not_active Withdrawn
- 2001-04-26 GB GB0227573A patent/GB2380490B/en not_active Expired - Fee Related
- 2001-04-26 EP EP10175113A patent/EP2256187A1/en not_active Withdrawn
- 2001-04-26 AU AU5917001A patent/AU5917001A/xx active Pending
- 2001-04-26 KR KR1020027014467A patent/KR100729971B1/ko active IP Right Grant
- 2001-04-26 CN CN01811938A patent/CN1439049A/zh active Pending
- 2001-04-26 WO PCT/US2001/013471 patent/WO2001081549A2/en active Application Filing
- 2001-05-31 US US09/872,182 patent/US6506574B1/en not_active Expired - Lifetime
-
2002
- 2002-10-24 IL IL152481A patent/IL152481A/en not_active IP Right Cessation
-
2006
- 2006-08-07 AU AU2006203395A patent/AU2006203395A1/en not_active Abandoned
-
2012
- 2012-06-21 JP JP2012139735A patent/JP5750081B2/ja not_active Expired - Fee Related
Non-Patent Citations (3)
Title |
---|
American Journal of Pathology Vol.150(2):591-602 (1997. 2.) |
Exp. Cell. Res. Vol.217(1):22-30 (1995. 3.) |
Molecular Medicine Vol.6(2):88-95 (2000. 2.) |
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JP2012205592A (ja) | 2012-10-25 |
JP5750081B2 (ja) | 2015-07-15 |
GB2380490A (en) | 2003-04-09 |
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CN102031241A (zh) | 2011-04-27 |
JP2003530879A (ja) | 2003-10-21 |
AU2006203395A1 (en) | 2006-08-31 |
US6506574B1 (en) | 2003-01-14 |
CA2407505A1 (en) | 2001-11-01 |
CN1439049A (zh) | 2003-08-27 |
GB0227573D0 (en) | 2002-12-31 |
US6458589B1 (en) | 2002-10-01 |
IL152481A (en) | 2010-11-30 |
GB2380490B (en) | 2004-12-29 |
CA2407505C (en) | 2007-10-23 |
IL152481A0 (en) | 2003-05-29 |
CN102031241B (zh) | 2016-02-24 |
EP1280887A2 (en) | 2003-02-05 |
AU5917001A (en) | 2001-11-07 |
KR20030032941A (ko) | 2003-04-26 |
WO2001081549A3 (en) | 2002-05-23 |
WO2001081549A2 (en) | 2001-11-01 |
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