SG11201605785XA - Stem cell-derived hepatocytes in co-culture and uses thereof - Google Patents

Stem cell-derived hepatocytes in co-culture and uses thereof

Info

Publication number
SG11201605785XA
SG11201605785XA SG11201605785XA SG11201605785XA SG11201605785XA SG 11201605785X A SG11201605785X A SG 11201605785XA SG 11201605785X A SG11201605785X A SG 11201605785XA SG 11201605785X A SG11201605785X A SG 11201605785XA SG 11201605785X A SG11201605785X A SG 11201605785XA
Authority
SG
Singapore
Prior art keywords
culture
stem cell
derived hepatocytes
hepatocytes
derived
Prior art date
Application number
SG11201605785XA
Inventor
Salman R Khetani
Dustin R Berger
Brenton R Ware
Original Assignee
Univ Colorado State Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Colorado State Res Found filed Critical Univ Colorado State Res Found
Publication of SG11201605785XA publication Critical patent/SG11201605785XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
SG11201605785XA 2014-01-14 2015-01-14 Stem cell-derived hepatocytes in co-culture and uses thereof SG11201605785XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461927285P 2014-01-14 2014-01-14
PCT/US2015/011363 WO2015108944A2 (en) 2014-01-14 2015-01-14 Stem cell-derived hepatocytes in co-culture and uses thereof

Publications (1)

Publication Number Publication Date
SG11201605785XA true SG11201605785XA (en) 2016-08-30

Family

ID=53543608

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201605785XA SG11201605785XA (en) 2014-01-14 2015-01-14 Stem cell-derived hepatocytes in co-culture and uses thereof

Country Status (6)

Country Link
US (2) US10266806B2 (en)
EP (1) EP3094719A4 (en)
AU (1) AU2015206547A1 (en)
CA (1) CA2936838A1 (en)
SG (1) SG11201605785XA (en)
WO (1) WO2015108944A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3331540B1 (en) * 2015-08-05 2021-09-29 Colorado State University Research Foundation Engineered platforms to stabilize both hepatocytes and endothelial cellsin vitro
US20180321224A1 (en) 2015-11-11 2018-11-08 Colorado State University Research Foundation Compositions and methods for increasing hepatocyte functional lifetime in vitro
CA3032997A1 (en) * 2016-07-12 2018-01-18 EMULATE, Inc. Compositions and methods of cell attachment
CN110381968A (en) * 2017-02-01 2019-10-25 菲尼克松生物制品公司 The composition and method of coculture comprising liver cell
US11407979B2 (en) 2017-09-01 2022-08-09 Colorado State University Research Foundation Intermittent starvation techniques to prolong functional lifetime of liver cells in culture
WO2019060790A1 (en) * 2017-09-22 2019-03-28 Lifenet Health Plated hepatocytes and preparation and uses thereof
CN110564688B (en) * 2018-06-06 2022-08-26 中山大学中山眼科中心 Method for culturing limbal stromal stem cells without serum and inducing balling and differentiation in vitro
BR112022018092A2 (en) 2020-03-11 2022-11-22 Bit Bio Ltd METHOD OF GENERATING LIVER CELLS

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133030A (en) * 1997-05-14 2000-10-17 The General Hospital Corporation Co-cultivation of cells in a micropatterned configuration
WO1998051785A1 (en) * 1997-05-14 1998-11-19 The General Hospital Corporation Co-cultivation of cells in a micropatterned configuration
US7282366B2 (en) 2000-04-27 2007-10-16 Geron Corporation Hepatocytes for therapy and drug screening made from embryonic stem cells
US6458589B1 (en) * 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
KR100524990B1 (en) 2003-10-09 2005-10-31 삼성전자주식회사 Method for manufacturing a semiconductor memory device
EP2495310A3 (en) * 2005-05-24 2013-03-27 The Regents Of the University of California Microscale micropatterned engineered in vitro tissue
US8062632B2 (en) 2008-06-23 2011-11-22 National Yang-Ming University Systems and methods for making hepatocytes from extrahepatic somatic stem cells and use thereof
CA2730057A1 (en) 2008-07-07 2010-01-14 Mass Institute Of Technology Micropatterned co-culture systems as infectious disease analysis platforms
WO2010042669A2 (en) 2008-10-07 2010-04-15 Ipierian, Inc. Co-culture compositions and methods
US8921108B2 (en) 2009-04-21 2014-12-30 Academisch Ziekenhuis Bij De Universiteit Van Amsterdam Differentiated human liver cell cultures and their use in bioartificial liver systems
WO2012119012A1 (en) 2011-03-02 2012-09-07 New Jersey Institute Of Technology System and method for vascularized biomimetic 3-d tissue models
WO2013138348A1 (en) * 2012-03-12 2013-09-19 Hepregen Corporatiion Systems and methods for studying inflammation-drug interactions
US20150079673A1 (en) 2013-07-03 2015-03-19 Colorado State University Research Foundation Hepatocytes in co-culture and uses thereof

Also Published As

Publication number Publication date
AU2015206547A1 (en) 2016-07-28
EP3094719A4 (en) 2017-08-02
WO2015108944A2 (en) 2015-07-23
US10717966B2 (en) 2020-07-21
EP3094719A2 (en) 2016-11-23
US20190048315A1 (en) 2019-02-14
CA2936838A1 (en) 2015-07-23
US10266806B2 (en) 2019-04-23
WO2015108944A3 (en) 2015-11-05
WO2015108944A9 (en) 2015-12-23
US20150240203A1 (en) 2015-08-27

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