PL2494035T3 - Pluripotencjalne komórki macierzyste - Google Patents

Pluripotencjalne komórki macierzyste

Info

Publication number
PL2494035T3
PL2494035T3 PL10830437T PL10830437T PL2494035T3 PL 2494035 T3 PL2494035 T3 PL 2494035T3 PL 10830437 T PL10830437 T PL 10830437T PL 10830437 T PL10830437 T PL 10830437T PL 2494035 T3 PL2494035 T3 PL 2494035T3
Authority
PL
Poland
Prior art keywords
stem cells
pluripotent stem
pluripotent
cells
stem
Prior art date
Application number
PL10830437T
Other languages
English (en)
Inventor
Ramie Fung
Benjamin Fryer
Original Assignee
Janssen Biotech, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Biotech, Inc. filed Critical Janssen Biotech, Inc.
Publication of PL2494035T3 publication Critical patent/PL2494035T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/608—Lin28
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Transplantation (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
PL10830437T 2009-10-29 2010-10-28 Pluripotencjalne komórki macierzyste PL2494035T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25614909P 2009-10-29 2009-10-29
PCT/US2010/054408 WO2011059725A2 (en) 2009-10-29 2010-10-28 Pluripotent stem cells
EP10830437.9A EP2494035B1 (en) 2009-10-29 2010-10-28 Pluripotent stem cells

Publications (1)

Publication Number Publication Date
PL2494035T3 true PL2494035T3 (pl) 2018-07-31

Family

ID=43925858

Family Applications (1)

Application Number Title Priority Date Filing Date
PL10830437T PL2494035T3 (pl) 2009-10-29 2010-10-28 Pluripotencjalne komórki macierzyste

Country Status (16)

Country Link
US (1) US20110104805A1 (pl)
EP (1) EP2494035B1 (pl)
JP (2) JP5957382B2 (pl)
KR (1) KR101861584B1 (pl)
CN (2) CN107058389A (pl)
AR (1) AR078805A1 (pl)
AU (1) AU2010319921A1 (pl)
BR (1) BR112012010136A2 (pl)
CA (1) CA2778817A1 (pl)
ES (1) ES2665006T3 (pl)
MX (1) MX350966B (pl)
PH (1) PH12012500818A1 (pl)
PL (1) PL2494035T3 (pl)
RU (1) RU2012122025A (pl)
SG (1) SG10201500674YA (pl)
WO (1) WO2011059725A2 (pl)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
MX2010001335A (es) 2007-07-31 2010-06-02 Lifescan Inc Diferenciacion de celulas madre embrionarias humanas.
CN102046779A (zh) 2008-02-21 2011-05-04 森托科尔奥索生物科技公司 用于细胞粘附、培养和分离的方法、表面改性培养板和组合物
RU2533805C2 (ru) 2008-06-30 2014-11-20 Сентокор Орто Байотек Инк. Дифференцирование плюрипотентных стволовых клеток, представляющих собой линию клеток человека
CA2744234C (en) 2008-11-20 2018-11-06 Centocor Ortho Biotech Inc. Pluripotent stem cell culture on micro-carriers
AU2009316583B2 (en) 2008-11-20 2016-04-21 Janssen Biotech, Inc. Methods and compositions for cell attachment and cultivation on planar substrates
EP2456862A4 (en) 2009-07-20 2013-02-27 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
RU2701335C2 (ru) 2009-12-23 2019-09-25 Янссен Байотек, Инк. Способ получения популяции панкреатических эндокринных клеток, соэкспрессирующих nkx6.1 и инсулин, и способ лечения диабета
CN102791851B (zh) 2010-03-01 2017-07-14 詹森生物科技公司 纯化衍生自多能干细胞的细胞的方法
PH12013500367A1 (en) 2010-08-31 2013-04-01 Janssen Biotech Inc Differentiation of human embryonic stem cells
EP2794857B1 (en) 2011-12-22 2026-02-11 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
WO2013151725A1 (en) 2012-04-05 2013-10-10 The Regents Of The University Of California Regenerative sera cells and mesenchymal stem cells
KR102468315B1 (ko) 2012-06-08 2022-11-16 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 췌장 내분비 세포로의 분화
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
BR112015015701A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc diferenciação de células-tronco embrionárias humanas em células pancreáticas endócrinas com o uso de reguladores hb9
DK2938724T3 (da) 2012-12-31 2020-12-14 Janssen Biotech Inc Dyrkning af humane embryonale stamceller ved luft-væskegrænsefladen til differentiering til endokrine pankreasceller
AU2013370228B2 (en) 2012-12-31 2018-10-18 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
EP3008168B1 (en) 2013-06-11 2020-02-19 President and Fellows of Harvard College Sc- cells and compositions and methods for generating the same
SG11201609473XA (en) 2014-05-16 2016-12-29 Janssen Biotech Inc Use of small molecules to enhance mafa expression in pancreatic endocrine cells
EP3234110B1 (en) 2014-12-18 2024-02-28 President and Fellows of Harvard College METHODS FOR GENERATING STEM CELL-DERIVED ß CELLS AND USES THEREOF
US10253298B2 (en) 2014-12-18 2019-04-09 President And Fellows Of Harvard College Methods for generating stem cell-derived beta cells and methods of use thereof
US10443042B2 (en) 2014-12-18 2019-10-15 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
WO2016100035A1 (en) 2014-12-19 2016-06-23 Janssen Biotech, Inc. Suspension culturing of pluripotent stem cells
JP6847044B2 (ja) 2015-03-11 2021-03-24 シーシーエス・ベンチャーズ・リミテッド 肥満及び２型糖尿病（ｔ２ｄ）の治療のための膵内分泌前駆細胞療法
CN104988115A (zh) * 2015-07-14 2015-10-21 中国科学院过程工程研究所 喹啉或异喹啉类小分子化合物在人多能干细胞培养及冷冻保存中的应用
EP4450076A2 (en) 2016-04-14 2024-10-23 Janssen Biotech, Inc. Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
KR102785694B1 (ko) 2016-06-21 2025-03-21 얀센 바이오테크 인코포레이티드 글루코스-의존성 미토콘드리아 호흡 및 2-상 인슐린 분비 반응을 나타내는 인간 만능 줄기 세포 유래 기능성 베타 세포의 생성
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
JP7428653B2 (ja) 2017-11-15 2024-02-06 バーテックス ファーマシューティカルズ インコーポレイテッド 島細胞製造組成物および使用方法
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
CN110564674B (zh) * 2019-09-29 2021-05-11 广东工业大学 一种干细胞铺底工作液及其应用
WO2021202771A2 (en) * 2020-04-01 2021-10-07 Young Bruce K Compositions and methods utilizing amniotic fluid stem cells
AU2021296296A1 (en) * 2020-06-22 2023-01-19 Life Technologies Corporation Methods and compositions for cultivating pluripotent cell suspensions
CN115418343A (zh) * 2022-10-28 2022-12-02 深圳市俊元生物科技有限公司 从人视网膜色素上皮细胞中提取多能干细胞的方法

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3935067A (en) * 1974-11-22 1976-01-27 Wyo-Ben Products, Inc. Inorganic support for culture media
US4557264A (en) * 1984-04-09 1985-12-10 Ethicon Inc. Surgical filament from polypropylene blended with polyethylene
US5215893A (en) * 1985-10-03 1993-06-01 Genentech, Inc. Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US5089396A (en) * 1985-10-03 1992-02-18 Genentech, Inc. Nucleic acid encoding β chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US4737578A (en) * 1986-02-10 1988-04-12 The Salk Institute For Biological Studies Human inhibin
US5863531A (en) 1986-04-18 1999-01-26 Advanced Tissue Sciences, Inc. In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework
US5759830A (en) 1986-11-20 1998-06-02 Massachusetts Institute Of Technology Three-dimensional fibrous scaffold containing attached cells for producing vascularized tissue in vivo
CA1340581C (en) * 1986-11-20 1999-06-08 Joseph P. Vacanti Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices
US5567612A (en) * 1986-11-20 1996-10-22 Massachusetts Institute Of Technology Genitourinary cell-matrix structure for implantation into a human and a method of making
US5837539A (en) * 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
US5449383A (en) * 1992-03-18 1995-09-12 Chatelier; Ronald C. Cell growth substrates
GB9206861D0 (en) * 1992-03-28 1992-05-13 Univ Manchester Wound healing and treatment of fibrotic disorders
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
GB9310557D0 (en) * 1993-05-21 1993-07-07 Smithkline Beecham Plc Novel process and apparatus
US6001647A (en) * 1994-04-28 1999-12-14 Ixion Biotechnology, Inc. In vitro growth of functional islets of Langerhans and in vivo uses thereof
US6703017B1 (en) * 1994-04-28 2004-03-09 Ixion Biotechnology, Inc. Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US5834308A (en) * 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5718922A (en) * 1995-05-31 1998-02-17 Schepens Eye Research Institute, Inc. Intravitreal microsphere drug delivery and method of preparation
US5908782A (en) * 1995-06-05 1999-06-01 Osiris Therapeutics, Inc. Chemically defined medium for human mesenchymal stem cells
ES2285779T3 (es) * 1997-07-03 2007-11-16 Osiris Therapeutics, Inc. Celulas madre mesenquimatosas humanas de sangre periferica.
DK1015576T3 (da) * 1997-09-16 2005-08-29 Egea Biosciences Llc Fremgangsmåde til fuldstændig kemisk syntese og aggregering af gener og genomer
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
EP1025204A4 (en) * 1997-10-23 2001-02-28 Geron Corp Methods and materials for the growth of primate-derived primordial stem cells
CO4980885A1 (es) * 1997-12-29 2000-11-27 Ortho Mcneil Pharm Inc Compuestos de trifenilpropanamida utiles en el tratamiento de inflamaciones y metodos para preparar dicho compuesto
EP1066052B1 (en) * 1998-03-18 2006-02-01 Osiris Therapeutics, Inc. Mesenchymal stem cells for prevention and treatment of immune responses in transplantation
MY132496A (en) 1998-05-11 2007-10-31 Vertex Pharma Inhibitors of p38
US7410798B2 (en) * 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6413556B1 (en) * 1999-01-08 2002-07-02 Sky High, Llc Aqueous anti-apoptotic compositions
US6458593B1 (en) * 1999-01-21 2002-10-01 Vitro Diagnostics, Inc. Immortalized cell lines and methods of making the same
US6815203B1 (en) * 1999-06-23 2004-11-09 Joslin Diabetes Center, Inc. Methods of making pancreatic islet cells
US6333029B1 (en) * 1999-06-30 2001-12-25 Ethicon, Inc. Porous tissue scaffoldings for the repair of regeneration of tissue
US6306424B1 (en) * 1999-06-30 2001-10-23 Ethicon, Inc. Foam composite for the repair or regeneration of tissue
US6685936B2 (en) * 1999-10-12 2004-02-03 Osiris Therapeutics, Inc. Suppressor cells induced by culture with mesenchymal stem cells for treatment of immune responses in transplantation
US7005252B1 (en) * 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US6436704B1 (en) * 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
US6458589B1 (en) * 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
CN100525768C (zh) 2000-10-23 2009-08-12 史密丝克莱恩比彻姆公司 新化合物
US6599323B2 (en) 2000-12-21 2003-07-29 Ethicon, Inc. Reinforced tissue implants and methods of manufacture and use
US6656488B2 (en) * 2001-04-11 2003-12-02 Ethicon Endo-Surgery, Inc. Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering
US6626950B2 (en) * 2001-06-28 2003-09-30 Ethicon, Inc. Composite scaffold with post anchor for the repair and regeneration of tissue
US6617152B2 (en) * 2001-09-04 2003-09-09 Corning Inc Method for creating a cell growth surface on a polymeric substrate
CA2470539C (en) * 2001-12-07 2011-10-04 Geron Corporation Islet cells from human embryonic stem cells
US6987110B2 (en) * 2002-06-05 2006-01-17 Janssen Pharmaceutica N.V. Substituted pyrrolines as kinase inhibitors
EP1539928A4 (en) * 2002-09-06 2006-09-06 Amcyte Inc CD56-POSITIVE HUMAN ADULTS PANCREATIC ENDOCRINE PRECURSOR CELLS
US20040062753A1 (en) 2002-09-27 2004-04-01 Alireza Rezania Composite scaffolds seeded with mammalian cells
US7592175B2 (en) * 2002-12-16 2009-09-22 Technion Research & Development Foundation Ltd. Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and cell cultures prepared using same
US20090203141A1 (en) * 2003-05-15 2009-08-13 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents
CA2530732C (en) * 2003-06-27 2015-03-31 DePuy Synthes Products, Inc. Regeneration and repair of neural tissue using postpartum-derived cells
WO2005017117A2 (en) * 2003-08-14 2005-02-24 Martin Haas Multipotent amniotic fetal stem cells (mafsc) and banking of same
US7157275B2 (en) * 2003-08-15 2007-01-02 Becton, Dickinson And Company Peptides for enhanced cell attachment and growth
SG149061A1 (en) * 2003-12-23 2009-01-29 Cythera Inc Definitive endoderm
WO2005097977A2 (en) * 2004-04-01 2005-10-20 Wisconsin Alumni Research Foundation Differentiation of stem cells to endoderm and pancreatic lineage
JP5420837B2 (ja) * 2004-09-08 2014-02-19 ウィスコンシン アラムニ リサーチ ファンデーション 胚幹細胞の培地及び培養
KR20070058584A (ko) * 2004-09-08 2007-06-08 위스콘신 얼럼나이 리서어치 화운데이션 인간 배아 줄기 세포의 배양
KR20080024194A (ko) * 2005-06-10 2008-03-17 아이알엠 엘엘씨 배아 줄기 세포의 다능성을 유지하는 화합물
US7695965B2 (en) * 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
CA2893679C (en) * 2006-06-26 2018-10-30 Lifescan, Inc. Conditioned medium for pluripotent stem cell culture
WO2008013664A2 (en) * 2006-07-26 2008-01-31 Cythera, Inc. Methods of producing pancreatic hormones
JP5813321B2 (ja) * 2007-03-23 2015-11-17 ウィスコンシン アラムニ リサーチ ファンデーション 体細胞の再プログラム化
CN101617043B (zh) * 2007-10-31 2014-03-12 国立大学法人京都大学 核重编程方法
US20110151447A1 (en) * 2007-11-06 2011-06-23 Children's Medical Center Corporation Method to produce induced pluripotent stem (ips) cells from non-embryonic human cells
WO2009096049A1 (ja) * 2008-02-01 2009-08-06 Kyoto University 人工多能性幹細胞由来分化細胞
US20100330677A1 (en) * 2008-02-11 2010-12-30 Cambridge Enterprise Limited Improved Reprogramming of Mammalian Cells, and Cells Obtained

Also Published As

Publication number Publication date
WO2011059725A2 (en) 2011-05-19
MX2012004999A (es) 2012-09-28
EP2494035A2 (en) 2012-09-05
JP2013509184A (ja) 2013-03-14
BR112012010136A2 (pt) 2015-09-15
JP5957382B2 (ja) 2016-07-27
SG10201500674YA (en) 2015-04-29
AR078805A1 (es) 2011-12-07
EP2494035B1 (en) 2018-02-28
EP2494035A4 (en) 2013-09-25
US20110104805A1 (en) 2011-05-05
ES2665006T3 (es) 2018-04-24
PH12012500818A1 (en) 2019-11-29
CA2778817A1 (en) 2011-05-19
KR101861584B1 (ko) 2018-05-28
JP2016127828A (ja) 2016-07-14
WO2011059725A3 (en) 2011-07-21
RU2012122025A (ru) 2013-12-10
KR20120099241A (ko) 2012-09-07
MX350966B (es) 2017-09-26
CN107058389A (zh) 2017-08-18
CN102695790A (zh) 2012-09-26
JP6227622B2 (ja) 2017-11-08
AU2010319921A1 (en) 2012-05-17

Similar Documents

Publication Publication Date Title
SG10201500674YA (en) Pluripotent stem cells
IL247310B (en) Mesenchymal stem cell differentiation
SG10201403202XA (en) Pre-natal mesenchymal stem cells
IL228818A0 (en) Stem cell cultures
ZA201008391B (en) Pluripotent cells
IL216624A0 (en) Fucosylation-deficient cells
ZA201100758B (en) Differentiation of pluripotent stem cells
GB0908613D0 (en) T Cell Reseptors
GB0814249D0 (en) Uses of mesenchymal stem cells
GB201202848D0 (en) Differemtiaiotn of human embryonic stem cells
ZA201000715B (en) Single pluripotent stem cell culture
EP2601290A4 (en) SOMATIC STEM CELLS
PL2456863T3 (pl) Pluripotencjalne komórki macierzyste otrzymywane z miazgi zęba
GB0920775D0 (en) Cells
ZA201202939B (en) Whole cell biocatalyst
EP2294182A4 (en) CULTS OF MULTIPOTENT STEM CELLS
GB0919773D0 (en) Induced pluripotent stem cell
GB0918471D0 (en) Stem cells
GB0916476D0 (en) Stem cell culture methods
GB0901069D0 (en) Stem cell culture methods
GB0818256D0 (en) stem cells
TWI370178B (en) Cell markers for human embryonic stem cells
GB0915268D0 (en) Cell culture
GB0922428D0 (en) Cell culture
GB0908223D0 (en) Cells