BR112012010136A2 - células -tronco pluripotentes - Google Patents
células -tronco pluripotentesInfo
- Publication number
- BR112012010136A2 BR112012010136A2 BR112012010136A BR112012010136A BR112012010136A2 BR 112012010136 A2 BR112012010136 A2 BR 112012010136A2 BR 112012010136 A BR112012010136 A BR 112012010136A BR 112012010136 A BR112012010136 A BR 112012010136A BR 112012010136 A2 BR112012010136 A2 BR 112012010136A2
- Authority
- BR
- Brazil
- Prior art keywords
- stem cells
- pluripotent stem
- provides methods
- present
- cells
- Prior art date
Links
- 210000001778 pluripotent stem cell Anatomy 0.000 title abstract 4
- 238000000034 method Methods 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000001177 retroviral effect Effects 0.000 abstract 1
- 210000001082 somatic cell Anatomy 0.000 abstract 1
- 238000001890 transfection Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/605—Nanog
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/608—Lin28
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- C12N2510/00—Genetically modified cells
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
- C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
- C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Transplantation (AREA)
- Developmental Biology & Embryology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Virology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
células -tronco pluripotentes. a presente invenção apresente métodos para produzir células-tronco pluripotentes. em particular, a presente invenção apresenta métodos para produzir células-tronco pluripotentes a partir de células somáticas sem o uso de camada alimentadora de célula ou um agente que aumenta a eficiência de transfecção retroviral.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25614909P | 2009-10-29 | 2009-10-29 | |
PCT/US2010/054408 WO2011059725A2 (en) | 2009-10-29 | 2010-10-28 | Pluripotent stem cells |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112012010136A2 true BR112012010136A2 (pt) | 2015-09-15 |
Family
ID=43925858
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112012010136A BR112012010136A2 (pt) | 2009-10-29 | 2010-10-28 | células -tronco pluripotentes |
Country Status (15)
Country | Link |
---|---|
US (1) | US20110104805A1 (pt) |
EP (1) | EP2494035B1 (pt) |
JP (2) | JP5957382B2 (pt) |
KR (1) | KR101861584B1 (pt) |
CN (2) | CN107058389A (pt) |
AR (1) | AR078805A1 (pt) |
AU (1) | AU2010319921A1 (pt) |
BR (1) | BR112012010136A2 (pt) |
CA (1) | CA2778817A1 (pt) |
ES (1) | ES2665006T3 (pt) |
MX (1) | MX350966B (pt) |
PL (1) | PL2494035T3 (pt) |
RU (1) | RU2012122025A (pt) |
SG (1) | SG10201500674YA (pt) |
WO (1) | WO2011059725A2 (pt) |
Families Citing this family (33)
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US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
RU2473685C2 (ru) | 2007-07-31 | 2013-01-27 | Лайфскен, Инк. | Дифференцировка человеческих эмбриональных стволовых клеток |
KR20190057164A (ko) | 2008-02-21 | 2019-05-27 | 얀센 바이오테크 인코포레이티드 | 세포 부착, 배양 및 탈리를 위한 방법, 표면 개질 플레이트 및 조성물 |
JP5734183B2 (ja) | 2008-06-30 | 2015-06-17 | ヤンセン バイオテツク,インコーポレーテツド | 多能性幹細胞の分化 |
BRPI0920956A2 (pt) | 2008-11-20 | 2015-08-18 | Centocor Ortho Biotech Inc | Cultura de células-tronco pluripotentes em microveículos |
RU2547925C2 (ru) | 2008-11-20 | 2015-04-10 | Сентокор Орто Байотек Инк. | Способы и композиции для закрепления и культивирования клеток на плоских носителях |
WO2011011300A2 (en) | 2009-07-20 | 2011-01-27 | Centocor Ortho Biotech Inc. | Differentiation of human embryonic stem cells |
RU2701335C2 (ru) | 2009-12-23 | 2019-09-25 | Янссен Байотек, Инк. | Способ получения популяции панкреатических эндокринных клеток, соэкспрессирующих nkx6.1 и инсулин, и способ лечения диабета |
CA2791476C (en) | 2010-03-01 | 2020-06-30 | Janssen Biotech, Inc. | Methods for purifying cells derived from pluripotent stem cells |
CN103221536B (zh) | 2010-08-31 | 2016-08-31 | 詹森生物科技公司 | 人胚胎干细胞的分化 |
EP2794857A4 (en) | 2011-12-22 | 2015-07-08 | Janssen Biotech Inc | DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS IN SINGLE INSULIN POSITIVE CELLS |
US20150064141A1 (en) | 2012-04-05 | 2015-03-05 | The Regents Of The University Of California | Regenerative sera cells and mesenchymal stem cells |
EP3450542B1 (en) | 2012-06-08 | 2021-09-01 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into pancreatic endocrine cells |
RU2658488C2 (ru) | 2012-12-31 | 2018-06-21 | Янссен Байотек, Инк. | Способ получения клеток, экспрессирующих маркеры, характерные для панкреатических эндокринных клеток |
US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
CA2896658C (en) | 2012-12-31 | 2021-06-22 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators |
WO2014106141A1 (en) | 2012-12-31 | 2014-07-03 | Janssen Biotech, Inc. | Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells |
MX2015017103A (es) | 2013-06-11 | 2016-11-07 | Harvard College | Celulas beta derivadas de células madre ( sc-beta ) y composiciones y métodos para generarlas. |
KR102162138B1 (ko) | 2014-05-16 | 2020-10-06 | 얀센 바이오테크 인코포레이티드 | 췌장 내분비 세포에서 mafa 발현을 향상시키기 위한 소분자의 용도 |
WO2016100930A1 (en) | 2014-12-18 | 2016-06-23 | President And Fellows Of Harvard College | Methods for generating stem cell-derived b cells and methods of use thereof |
US10443042B2 (en) | 2014-12-18 | 2019-10-15 | President And Fellows Of Harvard College | Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof |
US10190096B2 (en) | 2014-12-18 | 2019-01-29 | President And Fellows Of Harvard College | Methods for generating stem cell-derived β cells and uses thereof |
SG11201704961VA (en) | 2014-12-19 | 2017-07-28 | Janssen Biotech Inc | Suspension culturing of pluripotent stem cells |
CN107530379A (zh) | 2015-03-11 | 2018-01-02 | 佩特尼资源有限公司 | 用于治疗肥胖症和2型糖尿病(t2d)的胰腺内分泌祖细胞疗法 |
CN104988115A (zh) * | 2015-07-14 | 2015-10-21 | 中国科学院过程工程研究所 | 喹啉或异喹啉类小分子化合物在人多能干细胞培养及冷冻保存中的应用 |
MA45479A (fr) | 2016-04-14 | 2019-02-20 | Janssen Biotech Inc | Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen |
MA45502A (fr) | 2016-06-21 | 2019-04-24 | Janssen Biotech Inc | Génération de cellules bêta fonctionnelles dérivées de cellules souches pluripotentes humaines ayant une respiration mitochondriale glucose-dépendante et une réponse en sécrétion d'insuline en deux phases |
US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
BR112020009275A2 (pt) | 2017-11-15 | 2020-10-27 | Semma Therapeutics, Inc. | composições de fabricação de célula de ilhota e métodos de uso |
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-
2010
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- 2010-10-28 SG SG10201500674YA patent/SG10201500674YA/en unknown
- 2010-10-28 RU RU2012122025/10A patent/RU2012122025A/ru not_active Application Discontinuation
- 2010-10-28 MX MX2012004999A patent/MX350966B/es active IP Right Grant
- 2010-10-28 KR KR1020127013705A patent/KR101861584B1/ko active IP Right Grant
- 2010-10-28 EP EP10830437.9A patent/EP2494035B1/en not_active Not-in-force
- 2010-10-28 US US12/913,834 patent/US20110104805A1/en not_active Abandoned
- 2010-10-28 CA CA2778817A patent/CA2778817A1/en not_active Abandoned
- 2010-10-28 WO PCT/US2010/054408 patent/WO2011059725A2/en active Application Filing
- 2010-10-28 BR BR112012010136A patent/BR112012010136A2/pt not_active Application Discontinuation
- 2010-10-28 CN CN201611048756.9A patent/CN107058389A/zh active Pending
- 2010-10-28 JP JP2012537026A patent/JP5957382B2/ja not_active Expired - Fee Related
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- 2010-10-28 AU AU2010319921A patent/AU2010319921A1/en not_active Abandoned
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WO2011059725A3 (en) | 2011-07-21 |
CA2778817A1 (en) | 2011-05-19 |
ES2665006T3 (es) | 2018-04-24 |
MX350966B (es) | 2017-09-26 |
MX2012004999A (es) | 2012-09-28 |
RU2012122025A (ru) | 2013-12-10 |
US20110104805A1 (en) | 2011-05-05 |
WO2011059725A2 (en) | 2011-05-19 |
JP6227622B2 (ja) | 2017-11-08 |
CN107058389A (zh) | 2017-08-18 |
JP2013509184A (ja) | 2013-03-14 |
JP2016127828A (ja) | 2016-07-14 |
EP2494035B1 (en) | 2018-02-28 |
AR078805A1 (es) | 2011-12-07 |
JP5957382B2 (ja) | 2016-07-27 |
CN102695790A (zh) | 2012-09-26 |
AU2010319921A1 (en) | 2012-05-17 |
SG10201500674YA (en) | 2015-04-29 |
PL2494035T3 (pl) | 2018-07-31 |
KR101861584B1 (ko) | 2018-05-28 |
KR20120099241A (ko) | 2012-09-07 |
EP2494035A2 (en) | 2012-09-05 |
EP2494035A4 (en) | 2013-09-25 |
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