JPS59166086A - 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法 - Google Patents

新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Info

Publication number
JPS59166086A
JPS59166086A JP58038439A JP3843983A JPS59166086A JP S59166086 A JPS59166086 A JP S59166086A JP 58038439 A JP58038439 A JP 58038439A JP 3843983 A JP3843983 A JP 3843983A JP S59166086 A JPS59166086 A JP S59166086A
Authority
JP
Japan
Prior art keywords
plasmid
prochymosin
gene
dna
codon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP58038439A
Other languages
English (en)
Japanese (ja)
Other versions
JPS6240999B2 (enExample
Inventor
Teruhiko Beppu
別府　輝彦
Takeshi Uozumi
魚住　武司
Katsuhiko Nishimori
克彦 西森
Norio Shimizu
清水　範夫
Yoshiyuki Kawaguchi
嘉之 川口
Shinsei Hidaka
日高　真誠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP58038439A priority Critical patent/JPS59166086A/ja
Priority to DK136184A priority patent/DK136184A/da
Priority to NZ207414A priority patent/NZ207414A/en
Priority to EP84102451A priority patent/EP0121775A1/en
Priority to AU25357/84A priority patent/AU572827B2/en
Priority to FI840918A priority patent/FI86886C/fi
Priority to BR8401049A priority patent/BR8401049A/pt
Priority to IE559/84A priority patent/IE57264B1/en
Priority to AR84295974A priority patent/AR241795A1/es
Publication of JPS59166086A publication Critical patent/JPS59166086A/ja
Publication of JPS6240999B2 publication Critical patent/JPS6240999B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
JP58038439A 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法 Granted JPS59166086A (ja)

Priority Applications (9)

Application Number Priority Date Filing Date Title
JP58038439A JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法
DK136184A DK136184A (da) 1983-03-09 1984-02-29 Fremgangsmaade til udtrykkelse af et gen, der koder for prochymosin, i e.coli-vaertsceller og udtrykkelsesplasmid til brug ved fremgangsmaaden
NZ207414A NZ207414A (en) 1983-03-09 1984-03-07 Expression of prorennin (prochymosin) using recombinant plasmids in an e. coli host
EP84102451A EP0121775A1 (en) 1983-03-09 1984-03-07 Novel expression plasmids and their use in the method for expressing prochymosin coding gene in E. coli
AU25357/84A AU572827B2 (en) 1983-03-09 1984-03-07 Recombinant plasmid containing prochymosin coding gene
FI840918A FI86886C (fi) 1983-03-09 1984-03-07 Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli
BR8401049A BR8401049A (pt) 1983-03-09 1984-03-08 Processo para expressar um gene de codificacao de proquimosina em celulas hospedeiras de e.coli.plasmideo de expressao,gene de proquimosina e processo para fazer plasmideos
IE559/84A IE57264B1 (en) 1983-03-09 1984-03-08 Novel expression plasmids and their use in the method for expressing prochymosin coding gene in e.coli
AR84295974A AR241795A1 (es) 1983-03-09 1984-03-09 Metodo para expresar un gen que codifica proquimosina; plasmidos aplicados en ese metodo; metodo para obtenerlos; el gen incorporado a esos plasmidos.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP58038439A JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Publications (2)

Publication Number Publication Date
JPS59166086A true JPS59166086A (ja) 1984-09-19
JPS6240999B2 JPS6240999B2 (enExample) 1987-09-01

Family

ID=12525334

Family Applications (1)

Application Number Title Priority Date Filing Date
JP58038439A Granted JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Country Status (9)

Country Link
EP (1) EP0121775A1 (enExample)
JP (1) JPS59166086A (enExample)
AR (1) AR241795A1 (enExample)
AU (1) AU572827B2 (enExample)
BR (1) BR8401049A (enExample)
DK (1) DK136184A (enExample)
FI (1) FI86886C (enExample)
IE (1) IE57264B1 (enExample)
NZ (1) NZ207414A (enExample)

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EP0123928A3 (en) * 1983-03-31 1986-02-05 Codon Genetic Engineering Laboratories Recombinant dna coding for a polypeptide displaying milk clotting activity
US4721673A (en) * 1983-09-01 1988-01-26 Genex Corporation Recovery and activation process for microbially produced calf prochymosin
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
ATE158816T1 (de) 1990-11-26 1997-10-15 Genetics Inst Expression von pace in wirtszellen und verfahren zu dessen verwendung
US5629167A (en) 1994-04-19 1997-05-13 Biocine S.P.A. Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
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Citations (6)

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Publication number Priority date Publication date Assignee Title
JPS57141287A (en) * 1981-01-16 1982-09-01 Koraborateibu Research Inc Rennin, preprorennin or prorennin gene obtained from recombined dna material and live cell containing gene
JPS589687A (ja) * 1981-06-17 1983-01-20 セルテク　リミテッド ポリペプチドの生産方法
JPS5832896A (ja) * 1981-08-24 1983-02-25 Teruhiko Beppu 複合プラスミド及びそれを含有する微生物
JPS58109499A (ja) * 1981-10-14 1983-06-29 ユニリ−バ−・ナ−ムロ−ゼ・ベンノ−トシヤ−プ 組換えｄｎａ
JPS5921392A (ja) * 1982-07-01 1984-02-03 ジエネツクス・コ−ポレイシヨン 仔牛キモシン
JPS59156285A (ja) * 1982-12-22 1984-09-05 ジエネンテツク・インコ−ポレイテツド 微生物により産生されるレンネツト、その産生に使用する発現ベクタ−及びレンネツトの産生方法

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
IE53517B1 (en) * 1981-06-17 1988-12-07 Celltech Ltd Process for the production of a polypeptide

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57141287A (en) * 1981-01-16 1982-09-01 Koraborateibu Research Inc Rennin, preprorennin or prorennin gene obtained from recombined dna material and live cell containing gene
JPS589687A (ja) * 1981-06-17 1983-01-20 セルテク　リミテッド ポリペプチドの生産方法
JPS5832896A (ja) * 1981-08-24 1983-02-25 Teruhiko Beppu 複合プラスミド及びそれを含有する微生物
JPS58109499A (ja) * 1981-10-14 1983-06-29 ユニリ−バ−・ナ−ムロ−ゼ・ベンノ−トシヤ−プ 組換えｄｎａ
JPS5921392A (ja) * 1982-07-01 1984-02-03 ジエネツクス・コ−ポレイシヨン 仔牛キモシン
JPS59156285A (ja) * 1982-12-22 1984-09-05 ジエネンテツク・インコ−ポレイテツド 微生物により産生されるレンネツト、その産生に使用する発現ベクタ−及びレンネツトの産生方法

Also Published As

Publication number Publication date
JPS6240999B2 (enExample) 1987-09-01
FI840918A0 (fi) 1984-03-07
FI86886C (fi) 1992-10-26
AR241795A1 (es) 1992-12-30
FI840918L (fi) 1984-09-10
AU2535784A (en) 1984-09-13
BR8401049A (pt) 1984-10-16
DK136184A (da) 1984-09-10
IE840559L (en) 1984-09-09
EP0121775A1 (en) 1984-10-17
NZ207414A (en) 1988-02-29
IE57264B1 (en) 1992-07-01
DK136184D0 (da) 1984-02-29
AU572827B2 (en) 1988-05-19
FI86886B (fi) 1992-07-15

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