JPS6240999B2 - - Google Patents

Info

Publication number
JPS6240999B2
JPS6240999B2 JP58038439A JP3843983A JPS6240999B2 JP S6240999 B2 JPS6240999 B2 JP S6240999B2 JP 58038439 A JP58038439 A JP 58038439A JP 3843983 A JP3843983 A JP 3843983A JP S6240999 B2 JPS6240999 B2 JP S6240999B2
Authority
JP
Japan
Prior art keywords
plasmid
prochymosin
dna
gene
ecori
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP58038439A
Other languages
English (en)
Japanese (ja)
Other versions
JPS59166086A (ja
Inventor
Teruhiko Betsupu
Takeshi Uozumi
Katsuhiko Nishimori
Norio Shimizu
Yoshuki Kawaguchi
Shinsei Hidaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP58038439A priority Critical patent/JPS59166086A/ja
Priority to DK136184A priority patent/DK136184A/da
Priority to NZ207414A priority patent/NZ207414A/en
Priority to EP84102451A priority patent/EP0121775A1/en
Priority to FI840918A priority patent/FI86886C/fi
Priority to AU25357/84A priority patent/AU572827B2/en
Priority to IE559/84A priority patent/IE57264B1/en
Priority to BR8401049A priority patent/BR8401049A/pt
Priority to AR84295974A priority patent/AR241795A1/es
Publication of JPS59166086A publication Critical patent/JPS59166086A/ja
Publication of JPS6240999B2 publication Critical patent/JPS6240999B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
JP58038439A 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法 Granted JPS59166086A (ja)

Priority Applications (9)

Application Number Priority Date Filing Date Title
JP58038439A JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法
DK136184A DK136184A (da) 1983-03-09 1984-02-29 Fremgangsmaade til udtrykkelse af et gen, der koder for prochymosin, i e.coli-vaertsceller og udtrykkelsesplasmid til brug ved fremgangsmaaden
NZ207414A NZ207414A (en) 1983-03-09 1984-03-07 Expression of prorennin (prochymosin) using recombinant plasmids in an e. coli host
EP84102451A EP0121775A1 (en) 1983-03-09 1984-03-07 Novel expression plasmids and their use in the method for expressing prochymosin coding gene in E. coli
FI840918A FI86886C (fi) 1983-03-09 1984-03-07 Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli
AU25357/84A AU572827B2 (en) 1983-03-09 1984-03-07 Recombinant plasmid containing prochymosin coding gene
IE559/84A IE57264B1 (en) 1983-03-09 1984-03-08 Novel expression plasmids and their use in the method for expressing prochymosin coding gene in e.coli
BR8401049A BR8401049A (pt) 1983-03-09 1984-03-08 Processo para expressar um gene de codificacao de proquimosina em celulas hospedeiras de e.coli.plasmideo de expressao,gene de proquimosina e processo para fazer plasmideos
AR84295974A AR241795A1 (es) 1983-03-09 1984-03-09 Metodo para expresar un gen que codifica proquimosina; plasmidos aplicados en ese metodo; metodo para obtenerlos; el gen incorporado a esos plasmidos.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP58038439A JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Publications (2)

Publication Number Publication Date
JPS59166086A JPS59166086A (ja) 1984-09-19
JPS6240999B2 true JPS6240999B2 (enExample) 1987-09-01

Family

ID=12525334

Family Applications (1)

Application Number Title Priority Date Filing Date
JP58038439A Granted JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Country Status (9)

Country Link
EP (1) EP0121775A1 (enExample)
JP (1) JPS59166086A (enExample)
AR (1) AR241795A1 (enExample)
AU (1) AU572827B2 (enExample)
BR (1) BR8401049A (enExample)
DK (1) DK136184A (enExample)
FI (1) FI86886C (enExample)
IE (1) IE57264B1 (enExample)
NZ (1) NZ207414A (enExample)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0123928A3 (en) * 1983-03-31 1986-02-05 Codon Genetic Engineering Laboratories Recombinant dna coding for a polypeptide displaying milk clotting activity
US4721673A (en) * 1983-09-01 1988-01-26 Genex Corporation Recovery and activation process for microbially produced calf prochymosin
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
EP0785273A1 (en) 1990-11-26 1997-07-23 Genetics Institute, Inc. Paired basic amino acid converting enzyme and DNA sequence encoding it
US5629167A (en) 1994-04-19 1997-05-13 Biocine S.P.A. Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
BR9813930A (pt) 1997-11-06 2006-12-19 Chiron Spa antìgeno neisserial
ATE446368T1 (de) 1998-01-14 2009-11-15 Novartis Vaccines & Diagnostic Antigene aus neisseria meningitidis
GB9808932D0 (en) 1998-04-27 1998-06-24 Chiron Spa Polyepitope carrier protein
MX343752B (es) 1998-05-01 2016-11-22 Novartis Vaccines & Diagnostics Inc Antigenos de neisseria meningitidis y composiciones que los contienen.
NZ571167A (en) 1999-04-30 2010-05-28 Novartis Vaccines & Diagnostic Fragments from Neisseria protein ORF 953 and their use in medicaments and diagnostic reagents
GB9911683D0 (en) 1999-05-19 1999-07-21 Chiron Spa Antigenic peptides
GB9916529D0 (en) 1999-07-14 1999-09-15 Chiron Spa Antigenic peptides
PT2275551E (pt) 1999-10-29 2015-06-29 Novartis Vaccines & Diagnostic Péptidos antigénicos de neisseria
ES2588917T3 (es) 2000-01-17 2016-11-07 Glaxosmithkline Biologicals Sa Vacuna de VME suplementada contra meningococo
US7939087B2 (en) 2000-10-27 2011-05-10 Novartis Vaccines And Diagnostics, Inc. Nucleic acids and proteins from Streptococcus groups A & B
EP1334182B2 (en) 2000-11-06 2021-03-24 Chr. Hansen A/S Method of producing non-bovine chymosin and use hereof
GB0107661D0 (en) 2001-03-27 2001-05-16 Chiron Spa Staphylococcus aureus
GB0107658D0 (en) 2001-03-27 2001-05-16 Chiron Spa Streptococcus pneumoniae
ES2615362T3 (es) 2001-07-27 2017-06-06 Glaxosmithkline Biologicals Sa Adhesinas de meningococos
ES2386386T3 (es) 2001-12-12 2012-08-20 Novartis Vaccines And Diagnostics S.R.L. Inmunización contra Chlamydia trachomatis
AU2003239865A1 (en) 2002-05-24 2003-12-12 Restoragen Inc. Method for universal enzymatic production of bioactive peptides
AU2003243316A1 (en) 2002-05-24 2003-12-12 Nps Allelix Corp. Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides
CA2506318C (en) 2002-11-15 2011-07-12 Chiron Srl Unexpected surface proteins in neisseria meningitidis
GB0227346D0 (en) 2002-11-22 2002-12-31 Chiron Spa 741
GB0308198D0 (en) 2003-04-09 2003-05-14 Chiron Srl ADP-ribosylating bacterial toxin
EP2228379A1 (en) 2003-04-25 2010-09-15 North Carolina State University Lactobacillus acidophilus nucleic acid sequences encoding cell surface protein homologues and uses therefore
WO2005001057A2 (en) 2003-06-23 2005-01-06 North Carolina State University Lactobacillus acidophilus nucleic acids encoding fructo-oligosaccharide utilization compounds and uses thereof
US7829307B2 (en) 2003-11-21 2010-11-09 Nps Pharmaceuticals, Inc. Production of glucagon-like peptide 2
BRPI0507169A (pt) 2004-02-02 2007-06-26 Ambrx Inc polipeptìdeos do hormÈnio de crescimento humano modificados e seu usos
US7459289B2 (en) 2004-03-08 2008-12-02 North Carolina State University Lactobacillus acidophilus nucleic acid sequences encoding carbohydrate utilization-related proteins and uses therefor
EP1771205B1 (en) 2004-06-18 2016-10-26 Ambrx, Inc. Novel antigen-binding polypeptides and their uses
JP2008507280A (ja) 2004-07-21 2008-03-13 アンブレツクス・インコーポレイテツド 非天然コードアミノ酸を用いた生合成ポリペプチド
CA2594557C (en) 2004-12-22 2016-04-26 Ambrx, Inc. Modified human growth hormone
NZ555308A (en) 2004-12-22 2010-10-29 Ambrx Inc Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
JP2008537885A (ja) 2005-04-15 2008-10-02 ノース・キャロライナ・ステイト・ユニヴァーシティ 細菌の付着およびストレス耐性を修飾する方法および組成物
EP1951890A4 (en) 2005-11-16 2009-06-24 Ambrx Inc PROCESSES AND COMPOSITIONS WITH NON-NATURAL AMINO ACIDS
GB0524066D0 (en) 2005-11-25 2006-01-04 Chiron Srl 741 ii
DK2054431T3 (da) 2006-06-09 2012-01-02 Novartis Ag Konformere af bakterielle adhæsiner
KR20090060294A (ko) 2006-09-08 2009-06-11 암브룩스, 인코포레이티드 변형된 인간 혈장 폴리펩티드 또는 Ｆｃ 스캐폴드 및 그의 용도
CA2682147C (en) 2007-03-30 2017-08-08 Ambrx, Inc. Modified fgf-21 polypeptides and their uses
CA2703830A1 (en) 2007-11-20 2009-05-28 Ambrx, Inc. Modified insulin polypeptides and their uses
SG188143A1 (en) 2008-02-08 2013-03-28 Ambrx Inc Modified leptin polypeptides and their uses
AU2009215364B2 (en) 2008-02-21 2014-09-18 Glaxosmithkline Biologicals S.A. Meningococcal fHBP polypeptides
EP2318029B1 (en) 2008-07-23 2017-11-01 Ambrx, Inc. Modified bovine g-csf polypeptides and their uses
PE20110480A1 (es) 2008-09-26 2011-07-01 Ambrx Inc Microorganismos y vacunas dependientes de replicacion de aminoacidos no naturales
US8278418B2 (en) 2008-09-26 2012-10-02 Ambrx, Inc. Modified animal erythropoietin polypeptides and their uses
WO2011024072A2 (en) 2009-08-27 2011-03-03 Novartis Ag Hybrid polypeptides including meningococcal fhbp sequences
CA2779816A1 (en) 2009-10-27 2011-05-05 Novartis Ag Modified meningococcal fhbp polypeptides
JP2013515081A (ja) 2009-12-21 2013-05-02 アンブルックス，インコーポレイテッド 修飾されているブタのソマトトロピンポリペプチドおよびそれらの使用
EA201290541A1 (ru) 2009-12-21 2013-05-30 Амбркс, Инк. Модифицированные бычьи соматотропиновые полипептиды и их применение
CN103221420A (zh) 2010-05-19 2013-07-24 北卡罗莱纳州立大学 用于递送治疗性肽的组合物和方法
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
PE20140160A1 (es) 2010-08-17 2014-02-08 Ambrx Inc Polipeptidos de relaxina modificados y sus usos
TWI480288B (zh) 2010-09-23 2015-04-11 Lilly Co Eli 牛顆粒細胞群落刺激因子及其變體之調配物
RU2473683C1 (ru) * 2011-12-15 2013-01-27 Общество С Ограниченной Ответственностью "Фармапарк" СПОСОБ ПРОМЫШЛЕННОГО КУЛЬТИВИРОВАНИЯ ШТАММОВ E.coli, ПОЛУЧЕННЫХ НА ОСНОВЕ ШТАММА BL21(DE3), НЕСУЩЕГО ГЕН T7 RNA ПОЛИМЕРАЗЫ ПОД КОНТРОЛЕМ lacUV5 ПРОМОТОРА, С ПОВЫШЕННЫМ СИНТЕЗОМ БИОМАССЫ И ВЫХОДОМ ЦЕЛЕВОГО БЕЛКА В ТЕЛЬЦАХ ВКЛЮЧЕНИЯ
US10767156B2 (en) 2013-10-24 2020-09-08 Yeda Research And Development Co., Ltd. Polynucleotides encoding BREX system polypeptides and methods of using same
MX2017004947A (es) 2014-10-24 2017-06-29 Bristol Myers Squibb Co Polipeptidos del factor de crecimiento de fibroblastos 2 (fgf-21) modificados y usos de los mismos.
US10590426B2 (en) 2016-08-22 2020-03-17 Board Of Trustees Of Michigan State University Genetic control of cell size
IL250479A0 (en) 2017-02-06 2017-03-30 Sorek Rotem Genetically engineered cells expressing a disarm system that confers resistance to phages and methods for their production
SG10201912034UA (en) 2017-02-08 2020-02-27 Bristol Myers Squibb Co Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof
CN107881162A (zh) * 2017-12-25 2018-04-06 中诺生物科技发展江苏有限公司 一种工业化生产凝乳酶的方法
IL258467A (en) 2018-03-29 2018-07-04 Ilana Kolodkin Gal Methods of disrupting a biofilm and/or preventing formation of same
KR20210057124A (ko) 2018-09-11 2021-05-20 암브룩스, 인코포레이티드 인터류킨-2 폴리펩타이드 접합체 및 그의 용도
WO2020202142A2 (en) 2019-03-31 2020-10-08 Yeda Research And Development Co. Ltd. Anti-viral and anti-tumoral compounds
EP4117732A1 (en) 2020-03-11 2023-01-18 Ambrx, Inc. Interleukin-2 polypeptide conjugates and methods of use thereof
EP4228656A4 (en) 2020-08-18 2024-09-04 Yeda Research and Development Co. Ltd ANTI-VIRAL AND ANTI-TUMOR COMPOUNDS
IL288680A (en) 2021-12-05 2023-07-01 Yeda Res & Dev Genetically modified phages and their use
WO2025215636A1 (en) 2024-04-08 2025-10-16 Yeda Research And Development Co. Ltd. Anti-defense system polypeptides and uses thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
JPS589687A (ja) * 1981-06-17 1983-01-20 セルテク　リミテッド ポリペプチドの生産方法
IE53517B1 (en) * 1981-06-17 1988-12-07 Celltech Ltd Process for the production of a polypeptide
JPS5832896A (ja) * 1981-08-24 1983-02-25 Teruhiko Beppu 複合プラスミド及びそれを含有する微生物
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
ZA834095B (en) * 1982-07-01 1984-02-29 Genex Corp Bovine calf chymosin
IE56372B1 (en) * 1982-12-22 1991-07-03 Genentech Inc Microbially produced rennet methods for its production and plasmids used for its production

Also Published As

Publication number Publication date
DK136184A (da) 1984-09-10
FI86886C (fi) 1992-10-26
FI840918L (fi) 1984-09-10
BR8401049A (pt) 1984-10-16
FI840918A0 (fi) 1984-03-07
AU2535784A (en) 1984-09-13
IE57264B1 (en) 1992-07-01
IE840559L (en) 1984-09-09
AU572827B2 (en) 1988-05-19
JPS59166086A (ja) 1984-09-19
AR241795A1 (es) 1992-12-30
DK136184D0 (da) 1984-02-29
NZ207414A (en) 1988-02-29
EP0121775A1 (en) 1984-10-17
FI86886B (fi) 1992-07-15

Similar Documents

Publication Publication Date Title
FI86886C (fi) Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli
JP3592326B2 (ja) ユビキチン特異的プロテアーゼ
EP0174367B1 (en) Hosts and methods for producing recombinant products in high yields
US4711845A (en) Portable temperature-sensitive control cassette
JP3878207B2 (ja) ｏｓｍＢ プロモータで制御される発現プラスミド
JPH07108221B2 (ja) レンニンの製造方法
JPH0795881A (ja) キモシンの生産方法
JPH09135691A (ja) 牛のプレ成長および成長ホルモンの製造方法
JPH0376580A (ja) 大腸菌発現ベクターとそれを利用した抗ウイルス性タンパク質の製造方法
JPS62171695A (ja) 発光蛋白アクアリン遺伝子を用いるアクアリン活性を有する蛋白質の製造法
US4828988A (en) Hybrid polypeptides comprising somatocrinine and alpha1 -antitrypsin, method for their production from bacterial clones and use thereof for the production of somatocrinine
US6472196B1 (en) Amino acid sequence of L-phenylalanine ammonia-lyase
JP2971290B2 (ja) プラスミド及びそれで形質転換されたエシェリチア・コリ
US4757020A (en) Novel expression plasmids containing the full cDNA sequence of calf prochymosin
JPS589687A (ja) ポリペプチドの生産方法
JPH05268958A (ja) プロキモシンの製造方法
JP2525413B2 (ja) Ｌ−フェニルアラニン・アンモニアリア−ゼ発現用組換え体プラスミド及び該プラスミドを有する形質転換株
JPH03130088A (ja) 組み替えｄｎａ法によるポリペプチドの製造法
JP3091202B2 (ja) アセチルポリアミンアミドヒドロラーゼの製造法
JPS60188093A (ja) 枯草菌に於ける形質発現プラスミド
JP2560969B2 (ja) ヒトインスリン様成長因子ｉ遺伝子
JPH0644875B2 (ja) 組換え遺伝子産物の製造方法
JPS6237958B2 (enExample)
JPH0728746B2 (ja) 新規プラスミド、微生物細胞及びヒト免疫グロブリンＧ Ｆｃ領域蛋白質の製造法
JPH0755152B2 (ja) 組換え遺伝子産物の製法及び培地