NZ207414A - Expression of prorennin (prochymosin) using recombinant plasmids in an e. coli host - Google Patents

Expression of prorennin (prochymosin) using recombinant plasmids in an e. coli host

Info

Publication number
NZ207414A
NZ207414A NZ207414A NZ20741484A NZ207414A NZ 207414 A NZ207414 A NZ 207414A NZ 207414 A NZ207414 A NZ 207414A NZ 20741484 A NZ20741484 A NZ 20741484A NZ 207414 A NZ207414 A NZ 207414A
Authority
NZ
New Zealand
Prior art keywords
plasmid
prochymosin
gene
coli
dna
Prior art date
Application number
NZ207414A
Other languages
English (en)
Inventor
Beppu Teruhiko
Uozumi Takeshi
Nishimori Katsuhiko
Shimizu Norio
Kawaguchi Yoshiyuki
Hidaka Makota
Original Assignee
Beppu Teruhiko
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beppu Teruhiko filed Critical Beppu Teruhiko
Publication of NZ207414A publication Critical patent/NZ207414A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
NZ207414A 1983-03-09 1984-03-07 Expression of prorennin (prochymosin) using recombinant plasmids in an e. coli host NZ207414A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP58038439A JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Publications (1)

Publication Number Publication Date
NZ207414A true NZ207414A (en) 1988-02-29

Family

ID=12525334

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ207414A NZ207414A (en) 1983-03-09 1984-03-07 Expression of prorennin (prochymosin) using recombinant plasmids in an e. coli host

Country Status (9)

Country Link
EP (1) EP0121775A1 (enExample)
JP (1) JPS59166086A (enExample)
AR (1) AR241795A1 (enExample)
AU (1) AU572827B2 (enExample)
BR (1) BR8401049A (enExample)
DK (1) DK136184A (enExample)
FI (1) FI86886C (enExample)
IE (1) IE57264B1 (enExample)
NZ (1) NZ207414A (enExample)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO841276L (no) * 1983-03-31 1984-10-01 Codon Genetic Eng Lab Rekombinant dna-koding for et polypeptid som utviser melkesammenloepende aktivitet
US4721673A (en) * 1983-09-01 1988-01-26 Genex Corporation Recovery and activation process for microbially produced calf prochymosin
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
DK0574402T3 (da) 1990-11-26 1998-05-18 Chiron Corp Ekspression af PACE i værtsceller og fremgangsmåder til anvendelse deraf
US5629167A (en) 1994-04-19 1997-05-13 Biocine S.P.A. Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
WO1999024578A2 (en) 1997-11-06 1999-05-20 Chiron S.P.A. Neisserial antigens
CN1224708C (zh) 1998-01-14 2005-10-26 启龙股份公司 脑膜炎奈瑟氏球菌抗原
GB9808932D0 (en) 1998-04-27 1998-06-24 Chiron Spa Polyepitope carrier protein
EP2261350A3 (en) 1998-05-01 2012-01-04 Novartis Vaccines and Diagnostics, Inc. Neisseria meningitidis antigens and compositions
NZ530640A (en) 1999-04-30 2006-06-30 Chiron S Conserved neisserial antigens
GB9911683D0 (en) 1999-05-19 1999-07-21 Chiron Spa Antigenic peptides
GB9916529D0 (en) 1999-07-14 1999-09-15 Chiron Spa Antigenic peptides
PT2275552E (pt) 1999-10-29 2015-12-07 Glaxosmithkline Biolog Sa Péptidos antigénicos de neisseria
BR0107679A (pt) 2000-01-17 2004-07-06 Chiron Spa Vacina de vesìcula de membrana externa (omv) compreendendo proteìnas de membrana externa do grupo sérico b de neisseria meningitidis
ATE439372T1 (de) 2000-10-27 2009-08-15 Novartis Vaccines & Diagnostic Nukleinsäuren und proteine von gruppen a und b- streptokokken
WO2002036752A2 (en) 2000-11-06 2002-05-10 Chr. Hansen A/S Method of producing non-bovine chymosin and use thereof
GB0107658D0 (en) 2001-03-27 2001-05-16 Chiron Spa Streptococcus pneumoniae
GB0107661D0 (en) 2001-03-27 2001-05-16 Chiron Spa Staphylococcus aureus
PT2248822T (pt) 2001-07-27 2017-02-14 Glaxosmithkline Biologicals Sa Adesinas de meningococos
AU2002366592B2 (en) 2001-12-12 2008-01-10 Novartis Vaccines And Diagnostics S.R.L. Immunisation against Chlamydia trachomatis
CA2485695A1 (en) 2002-05-24 2003-12-04 Fred W. Wagner Method for universal enzymatic production of bioactive peptides
WO2003099854A2 (en) 2002-05-24 2003-12-04 Nps Allelix Corp. Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides
EP2279746B1 (en) 2002-11-15 2013-10-02 Novartis Vaccines and Diagnostics S.r.l. Surface proteins in neisseria meningitidis
GB0227346D0 (en) 2002-11-22 2002-12-31 Chiron Spa 741
GB0308198D0 (en) 2003-04-09 2003-05-14 Chiron Srl ADP-ribosylating bacterial toxin
AU2004235346A1 (en) 2003-04-25 2004-11-11 North Carolina State University Lactobacillus acidophilus nucleic acid sequences encoding cell surface protein homologues and uses therefore
DE602004032144D1 (de) 2003-06-23 2011-05-19 Univ North Carolina State Für fructooligosaccharid-nutzende verbindungen codierende nukleinsäuren aus lactobacillus acidophilus und verwendungen davon
CA2546319A1 (en) 2003-11-21 2005-07-28 Nps Allelix Corp. Production of glucagon like peptide 2 and analogs
NZ548255A (en) 2004-02-02 2010-10-29 Ambrx Inc Modified human interferon polypeptides and their uses
US7459289B2 (en) 2004-03-08 2008-12-02 North Carolina State University Lactobacillus acidophilus nucleic acid sequences encoding carbohydrate utilization-related proteins and uses therefor
CN102603895B (zh) 2004-06-18 2016-09-28 Ambrx公司 新颖抗原结合多肽和其用途
BRPI0512396A (pt) 2004-07-21 2008-03-11 Ambrx Inc polipeptìdeos biossintéticos utilizando aminoácidos codificados não naturalmente
NZ555308A (en) 2004-12-22 2010-10-29 Ambrx Inc Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
KR20070090023A (ko) 2004-12-22 2007-09-04 암브룩스, 인코포레이티드 변형 인간 성장 호르몬
CN102212543A (zh) 2005-04-15 2011-10-12 北卡罗来纳州大学 调节细菌的粘附和应激耐受力的方法和组合物
ES2547554T3 (es) 2005-11-16 2015-10-07 Ambrx, Inc. Métodos y composiciones que comprenden aminoácidos no naturales
GB0524066D0 (en) 2005-11-25 2006-01-04 Chiron Srl 741 ii
WO2009030978A2 (en) 2006-06-09 2009-03-12 Novartis Ag Conformers of bacterial adhesins
MX2009002523A (es) 2006-09-08 2009-03-20 Ambrx Inc Polipeptido de plasma humano modificado o andamios fc y sus usos.
AU2008232937B2 (en) 2007-03-30 2012-09-27 Ambrx, Inc. Modified FGF-21 polypeptides and their uses
EP2217265B1 (en) 2007-11-20 2017-05-10 Ambrx, Inc. Modified insulin polypeptides and their uses
NZ586947A (en) 2008-02-08 2012-11-30 Ambrx Inc Modified leptin polypeptides and their uses
BRPI0907843A2 (pt) 2008-02-21 2015-08-04 Novartis Ag Polipeptídeos de fhbp menigocócicos
UA103774C2 (uk) 2008-07-23 2013-11-25 Амбркс, Інк. Модифіковані поліпептиди бичачого гранулоцитарного колонієстимулювального фактора (g-csf) та їх застосування
BR122012024318A2 (pt) 2008-09-26 2019-07-30 Ambrx, Inc. Polipeptídeos modificados de eritropoetina animal e seus usos
MX357314B (es) 2008-09-26 2018-07-04 Ambrx Inc Microorganismos y vacunas dependientes de replicacion de aminoacidos no naturales.
CN102596240B (zh) 2009-08-27 2015-02-04 诺华股份有限公司 包括脑膜炎球菌fHBP序列的杂交多肽
BR112012010531A2 (pt) 2009-10-27 2019-09-24 Novartis Ag "polipeptídeos de modificação meningocócica fhbp"
EP2805965A1 (en) 2009-12-21 2014-11-26 Ambrx, Inc. Modified porcine somatotropin polypeptides and their uses
JP2013515080A (ja) 2009-12-21 2013-05-02 アンブルックス，インコーポレイテッド 修飾されているウシのソマトトロピンポリペプチドおよびそれらの使用
WO2011146715A1 (en) 2010-05-19 2011-11-24 North Carolina State University Compositions and methods for the delivery of therapeutic peptides
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
SMT201900495T1 (it) 2010-08-17 2019-11-13 Ambrx Inc Polipeptidi di relaxina modificati e loro usi
TWI480288B (zh) 2010-09-23 2015-04-11 Lilly Co Eli 牛顆粒細胞群落刺激因子及其變體之調配物
RU2473683C1 (ru) * 2011-12-15 2013-01-27 Общество С Ограниченной Ответственностью "Фармапарк" СПОСОБ ПРОМЫШЛЕННОГО КУЛЬТИВИРОВАНИЯ ШТАММОВ E.coli, ПОЛУЧЕННЫХ НА ОСНОВЕ ШТАММА BL21(DE3), НЕСУЩЕГО ГЕН T7 RNA ПОЛИМЕРАЗЫ ПОД КОНТРОЛЕМ lacUV5 ПРОМОТОРА, С ПОВЫШЕННЫМ СИНТЕЗОМ БИОМАССЫ И ВЫХОДОМ ЦЕЛЕВОГО БЕЛКА В ТЕЛЬЦАХ ВКЛЮЧЕНИЯ
US10767156B2 (en) 2013-10-24 2020-09-08 Yeda Research And Development Co., Ltd. Polynucleotides encoding BREX system polypeptides and methods of using same
CN114805531A (zh) 2014-10-24 2022-07-29 百时美施贵宝公司 修饰的fgf-21多肽及其用途
US10590426B2 (en) 2016-08-22 2020-03-17 Board Of Trustees Of Michigan State University Genetic control of cell size
IL250479A0 (en) 2017-02-06 2017-03-30 Sorek Rotem Genetically engineered cells expressing a disarm system that confers resistance to phages and methods for their production
TW201837051A (zh) 2017-02-08 2018-10-16 美商必治妥美雅史谷比公司 包含藥物動力學增強劑之經修飾之鬆弛素(relaxin)多肽及其用途
CN107881162A (zh) * 2017-12-25 2018-04-06 中诺生物科技发展江苏有限公司 一种工业化生产凝乳酶的方法
IL258467A (en) 2018-03-29 2018-07-04 Ilana Kolodkin Gal Methods of disrupting a biofilm and/or preventing formation of same
AU2019337610A1 (en) 2018-09-11 2021-05-06 Ambrx, Inc. Interleukin-2 polypeptide conjugates and their uses
US12419902B2 (en) 2019-03-31 2025-09-23 Yeda Research And Development Co. Ltd. Anti-viral and anti-tumoral compounds
EP4117732A1 (en) 2020-03-11 2023-01-18 Ambrx, Inc. Interleukin-2 polypeptide conjugates and methods of use thereof
EP4228656A4 (en) 2020-08-18 2024-09-04 Yeda Research and Development Co. Ltd ANTI-VIRAL AND ANTI-TUMOR COMPOUNDS
IL288680A (en) 2021-12-05 2023-07-01 Yeda Res & Dev Genetically modified phages and their use
WO2025215636A1 (en) 2024-04-08 2025-10-16 Yeda Research And Development Co. Ltd. Anti-defense system polypeptides and uses thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
JPS589687A (ja) * 1981-06-17 1983-01-20 セルテク　リミテッド ポリペプチドの生産方法
IE53517B1 (en) * 1981-06-17 1988-12-07 Celltech Ltd Process for the production of a polypeptide
JPS5832896A (ja) * 1981-08-24 1983-02-25 Teruhiko Beppu 複合プラスミド及びそれを含有する微生物
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
ZA834095B (en) * 1982-07-01 1984-02-29 Genex Corp Bovine calf chymosin
IE56372B1 (en) * 1982-12-22 1991-07-03 Genentech Inc Microbially produced rennet methods for its production and plasmids used for its production

Also Published As

Publication number Publication date
AU572827B2 (en) 1988-05-19
FI86886C (fi) 1992-10-26
IE840559L (en) 1984-09-09
IE57264B1 (en) 1992-07-01
EP0121775A1 (en) 1984-10-17
JPS6240999B2 (enExample) 1987-09-01
DK136184A (da) 1984-09-10
BR8401049A (pt) 1984-10-16
AR241795A1 (es) 1992-12-30
JPS59166086A (ja) 1984-09-19
DK136184D0 (da) 1984-02-29
AU2535784A (en) 1984-09-13
FI840918A0 (fi) 1984-03-07
FI86886B (fi) 1992-07-15
FI840918L (fi) 1984-09-10

Similar Documents

Publication Publication Date Title
NZ207414A (en) Expression of prorennin (prochymosin) using recombinant plasmids in an e. coli host
EP0198015B2 (en) Production of streptavidin-like polypeptides
US4666847A (en) Recombinant DNA means and method
EP0075444B1 (en) Methods and products for facile microbial expression of dna sequences
JPH09135691A (ja) 牛のプレ成長および成長ホルモンの製造方法
GB2100737A (en) A process for the production of a polypeptide
US5272254A (en) Production of streptavidin-like polypeptides
JPH074254B2 (ja) ヒト成長ホルモンの製法
US5260201A (en) Methods and products for facile microbial expression of DNA sequences
EP0260919B1 (en) L-phenylalanine ammonia lyase
US4880910A (en) Terminal methionyl bovine growth hormone and its use
CA1288072C (en) Expression plasmids containing the full cdna sequence of calf prochymosin
CA1283068C (en) Plasmid for expression of penicillin g amidase
JPH06503718A (ja) メチロトローフ酵母細胞におけるヒトリゾチムの産生とその効率的な分泌
JP2525413B2 (ja) Ｌ−フェニルアラニン・アンモニアリア−ゼ発現用組換え体プラスミド及び該プラスミドを有する形質転換株
WO2009054754A1 (en) PHINS21 RECOMBINANT PLASMID FOR ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN, AN Escherichia coli JM109/pHINS21 BACTERIA STRAIN AS A PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN AND A HUMAN PROINSULIN PRODUCING METHOD
US5489529A (en) DNA for expression of bovine growth hormone
JPH0286777A (ja) サル膵臓エラスターゼｉ
JPS6137099A (ja) 蛋白質の製造法
CA2160861A1 (en) Induction-free process for the recombinant preparation of glutarylamidase
JPH05268958A (ja) プロキモシンの製造方法
JPS62175173A (ja) ヒト・膵臓エラスタ−ゼ３ｂ
JPH06253838A (ja) ヒト・膵臓エラスターゼｉｉｉｂ
JPS60141290A (ja) 中性プロテア−ゼの構造遺伝子を含むｄνａフラグメントおよび中性プロテア−ゼ製造法
JPS63105681A (ja) Ｄｎａ塩基配列