FI86886C - Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli - Google Patents

Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli Download PDF

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Publication number
FI86886C
FI86886C FI840918A FI840918A FI86886C FI 86886 C FI86886 C FI 86886C FI 840918 A FI840918 A FI 840918A FI 840918 A FI840918 A FI 840918A FI 86886 C FI86886 C FI 86886C
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FI
Finland
Prior art keywords
plasmid
dna
ecori
map shown
prochymosin
Prior art date
Application number
FI840918A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI840918L (fi
FI840918A0 (fi
FI86886B (fi
Inventor
Takeshi Uozumi
Katsuhiko Nishimori
Norio Shimizu
Yoshiyuki Kawaguchi
Makoto Hidaka
Teruhiko Beppu
Original Assignee
Teruhiko Beppu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Teruhiko Beppu filed Critical Teruhiko Beppu
Publication of FI840918A0 publication Critical patent/FI840918A0/fi
Publication of FI840918L publication Critical patent/FI840918L/fi
Application granted granted Critical
Publication of FI86886B publication Critical patent/FI86886B/fi
Publication of FI86886C publication Critical patent/FI86886C/fi

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
FI840918A 1983-03-09 1984-03-07 Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli FI86886C (fi)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP58038439A JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法
JP3843983 1983-03-09

Publications (4)

Publication Number Publication Date
FI840918A0 FI840918A0 (fi) 1984-03-07
FI840918L FI840918L (fi) 1984-09-10
FI86886B FI86886B (fi) 1992-07-15
FI86886C true FI86886C (fi) 1992-10-26

Family

ID=12525334

Family Applications (1)

Application Number Title Priority Date Filing Date
FI840918A FI86886C (fi) 1983-03-09 1984-03-07 Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli

Country Status (9)

Country Link
EP (1) EP0121775A1 (enExample)
JP (1) JPS59166086A (enExample)
AR (1) AR241795A1 (enExample)
AU (1) AU572827B2 (enExample)
BR (1) BR8401049A (enExample)
DK (1) DK136184A (enExample)
FI (1) FI86886C (enExample)
IE (1) IE57264B1 (enExample)
NZ (1) NZ207414A (enExample)

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JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンcDΝAの全配列を有する新規な発現プラスミド
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US5629167A (en) 1994-04-19 1997-05-13 Biocine S.P.A. Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
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Also Published As

Publication number Publication date
DK136184A (da) 1984-09-10
FI840918L (fi) 1984-09-10
BR8401049A (pt) 1984-10-16
FI840918A0 (fi) 1984-03-07
AU2535784A (en) 1984-09-13
IE57264B1 (en) 1992-07-01
IE840559L (en) 1984-09-09
AU572827B2 (en) 1988-05-19
JPS59166086A (ja) 1984-09-19
AR241795A1 (es) 1992-12-30
DK136184D0 (da) 1984-02-29
NZ207414A (en) 1988-02-29
EP0121775A1 (en) 1984-10-17
FI86886B (fi) 1992-07-15
JPS6240999B2 (enExample) 1987-09-01

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Owner name: BEPPU, TERUHIKO