JPH051280B2 - - Google Patents
Info
- Publication number
- JPH051280B2 JPH051280B2 JP58186187A JP18618783A JPH051280B2 JP H051280 B2 JPH051280 B2 JP H051280B2 JP 58186187 A JP58186187 A JP 58186187A JP 18618783 A JP18618783 A JP 18618783A JP H051280 B2 JPH051280 B2 JP H051280B2
- Authority
- JP
- Japan
- Prior art keywords
- hbs antigen
- acid
- hbs
- antigen
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108091007433 antigens Proteins 0.000 claims description 95
- 102000036639 antigens Human genes 0.000 claims description 94
- 239000000427 antigen Substances 0.000 claims description 93
- 238000000034 method Methods 0.000 claims description 47
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 25
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 24
- 239000002253 acid Substances 0.000 claims description 15
- 239000002994 raw material Substances 0.000 claims description 14
- 108091005804 Peptidases Proteins 0.000 claims description 12
- 239000004365 Protease Substances 0.000 claims description 12
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 12
- 238000010828 elution Methods 0.000 claims description 11
- 239000002244 precipitate Substances 0.000 claims description 11
- 239000000356 contaminant Substances 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- 239000000287 crude extract Substances 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 239000013543 active substance Substances 0.000 claims description 7
- 238000001042 affinity chromatography Methods 0.000 claims description 7
- 238000005185 salting out Methods 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 6
- 238000011276 addition treatment Methods 0.000 claims description 6
- 150000002632 lipids Chemical class 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- 239000011324 bead Substances 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 235000006408 oxalic acid Nutrition 0.000 claims description 2
- 239000004094 surface-active agent Substances 0.000 claims description 2
- 238000011282 treatment Methods 0.000 claims description 2
- 238000002525 ultrasonication Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 description 19
- 238000004587 chromatography analysis Methods 0.000 description 15
- 230000000694 effects Effects 0.000 description 14
- 239000008363 phosphate buffer Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- 238000005199 ultracentrifugation Methods 0.000 description 11
- 239000000872 buffer Substances 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 10
- 229930006000 Sucrose Natural products 0.000 description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 8
- 238000005119 centrifugation Methods 0.000 description 8
- 239000005720 sucrose Substances 0.000 description 8
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 239000000499 gel Substances 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- 239000008280 blood Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 241000700721 Hepatitis B virus Species 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000006167 equilibration buffer Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 229960005486 vaccine Drugs 0.000 description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 238000010306 acid treatment Methods 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000002523 gelfiltration Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 241000282579 Pan Species 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 241001105470 Valenzuela Species 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- SPSXSWRZQFPVTJ-ZQQKUFEYSA-N hepatitis b vaccine Chemical compound C([C@H](NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCSC)C(=O)N[C@@H](CC1N=CN=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)OC(=O)CNC(=O)CNC(=O)[C@H](C)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@@H](N)CCCNC(N)=N)C1=CC=CC=C1 SPSXSWRZQFPVTJ-ZQQKUFEYSA-N 0.000 description 2
- 229940124736 hepatitis-B vaccine Drugs 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011076 safety test Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical group [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Description
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Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ HBsæåã粟補ããæ¹æ³ã«ãããŠãHBsAg
ãã³ãŒãããéºäŒåã§åœ¢è³ªè»¢æãããé µæ¯ã®å¹é€
ç©ãç Žå£ããŠåŸãããHBsæåç²æœåºç©ãHBs
æååææãšããŠçšãããããé žæ·»å åŠçããã€
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ããšã§ããåèšç¬¬ïŒé èšèŒã®æ¹æ³ã ïŒ é žæ·»å åŠçã«çšããé žããå¡©é žãç¡«é žãç
é žãé ¢é žãã·ãŠãŠé žã®äžããéžã°ããå°ãªããšã
ïŒçš®ã§ããåèšç¬¬ïŒé ãŸãã¯ç¬¬ïŒé èšèŒã®æ¹æ³ã ïŒ ãã€ãããã·ã¢ãã¿ã€ãã²ã«ã»ã¢ãã€ããã€
ã¯ãããã°ã©ãã€ãŒã«ãããŠæ®µé溶åºãŸãã¯æ¿åºŠ
åŸé 溶åºãè¡ãåèšç¬¬ïŒé èšèŒã®æ¹æ³ã ïŒ HBsæååææãé žæ·»å åŠçããã€ãã«ç¡«
å®å¡©æãè¡ãããŸãã¯è¡ããªãã§ãç¶ããŠãã€ã
ããã·ã¢ãã¿ã€ãã²ã«ã»ã¢ãã€ããã€ã¯ãããã°
ã©ãã€ãŒãè¡ãããããã¢ãŒãŒæŽ»æ§ç©è³ªãå®å šã«
é€å»ããHBsæåãåŸãåèšç¬¬ïŒé èšèŒã®æ¹æ³ã
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58186187A JPS6078999A (ja) | 1983-10-05 | 1983-10-05 | ïœæåã®ç²Ÿè£œæ¹æ³ |
| US06/656,315 US4612283A (en) | 1983-10-05 | 1984-10-01 | Method for purification of HBs antigen |
| EP84111917A EP0138167B1 (en) | 1983-10-05 | 1984-10-04 | Method for purification of hbs antigen |
| DE8484111917T DE3468623D1 (en) | 1983-10-05 | 1984-10-04 | Method for purification of hbs antigen |
| KR1019840006164A KR910008643B1 (ko) | 1983-10-05 | 1984-10-05 | Bí ê°ìŒ ë¹ë£šì€ íë©Žíì(HBs íì)ì ì ì ë°©ë² |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58186187A JPS6078999A (ja) | 1983-10-05 | 1983-10-05 | ïœæåã®ç²Ÿè£œæ¹æ³ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6078999A JPS6078999A (ja) | 1985-05-04 |
| JPH051280B2 true JPH051280B2 (ja) | 1993-01-07 |
Family
ID=16183908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58186187A Granted JPS6078999A (ja) | 1983-10-05 | 1983-10-05 | ïœæåã®ç²Ÿè£œæ¹æ³ |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4612283A (ja) |
| EP (1) | EP0138167B1 (ja) |
| JP (1) | JPS6078999A (ja) |
| KR (1) | KR910008643B1 (ja) |
| DE (1) | DE3468623D1 (ja) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE8402861L (sv) * | 1984-05-28 | 1985-11-29 | Stefan Svenson | Rening av biologiskt material |
| JPS61103895A (ja) * | 1984-10-26 | 1986-05-22 | Green Cross Corp:The | ïœïŒ¡ïœã®ç²Ÿè£œæ¹æ³ |
| CA1268437A (en) * | 1984-12-21 | 1990-05-01 | Haruo Fujita | Method for purification of hbc antigen and method for measurement of hbc antibody by using said purified hbc antigen |
| US5026828A (en) * | 1987-02-27 | 1991-06-25 | Merck & Co., Inc. | Method of purifying recombinant pres-1/S-2/S/S hepatitis B antigen from yeast |
| JP2560069B2 (ja) * | 1988-03-09 | 1996-12-04 | éªå°ä¹³æ¥æ ªåŒäŒç€Ÿ | ãšãªã¹ãããšãã³ã®è£œé æ¹æ³ |
| US5004688A (en) * | 1988-04-15 | 1991-04-02 | Phillips Petroleum Company | Purification of hepatitis proteins |
| JP2825647B2 (ja) * | 1989-02-07 | 1998-11-18 | ãã€ãªâãã¯ãããžãŒã»ãžãšãã©ã«ã»ã³ãŒãã¬ã€ã·ãšã³ | åèçã¯ã¯ãã³ã®è£œé åã³ç²Ÿè£œæ¹æ³ |
| JPH0743385B2 (ja) * | 1990-03-16 | 1995-05-15 | ç©æ°ŽååŠå·¥æ¥æ ªåŒäŒç€Ÿ | æ¢ æ¯æåã®è£œé æ³ |
| US5474900A (en) * | 1990-03-16 | 1995-12-12 | Sekisui Chemical Co., Ltd. | Process for preparing purified syphilis antigen from Treponema palljdum |
| JP2510629Y2 (ja) * | 1993-02-18 | 1996-09-18 | æ ªåŒäŒç€Ÿãã¹ã« | åååŒä¿æ¢çåä»æ© |
| CN1063343C (zh) * | 1993-04-27 | 2001-03-21 | äžåœç§åŠé¢äžæµ·çç©ååŠç 究æ | 氚端垊åè¡šé¢æåå³å®ç°çä¹åèçè¡šé¢æåèçœ |
| IL141130A0 (en) | 1998-08-14 | 2002-02-10 | Merck & Co Inc | Process for purifying human papilloma virus-like particles |
| JP6409528B2 (ja) | 2014-11-27 | 2018-10-24 | Jncæ ªåŒäŒç€Ÿ | ã¢ããåºãå«ãã€ãªã³äº€æåºãšããã«åºãå«ãçæ°Žæ§åºãšãæããå€åæ§ã»ã«ããŒã¹ç²ååã³ãããå«ãã¯ãããã°ã©ãã£ãŒæ äœãªãã³ã«ïœåèçãŠã£ã«ã¹ã®ãŠã£ã«ã¹æ§ç²åã®ç²Ÿè£œæ¹æ³ |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE52036B1 (en) * | 1979-05-24 | 1987-05-27 | Univ California | Non-passageable viruses |
| US4301250A (en) * | 1979-11-02 | 1981-11-17 | Merck & Co., Inc. | Method of producing hepatitis B surface antigen |
| EP0062574A3 (en) * | 1981-03-31 | 1982-12-29 | The Regents Of The University Of California | Virus protein synthesis |
| GB2102006B (en) * | 1981-06-16 | 1984-11-28 | Takeda Chemical Industries Ltd | Deoxyribonucleic acids, their production and use |
| GR76274B (ja) * | 1981-08-04 | 1984-08-04 | Univ California | |
| NZ201705A (en) * | 1981-08-31 | 1986-03-14 | Genentech Inc | Recombinant dna method for production of hepatitis b surface antigen in yeast |
| ZW18282A1 (en) * | 1981-08-31 | 1983-03-23 | Genentech Inc | Preparation of polypeptides in vertebrate cell culture |
| US4442205A (en) * | 1981-09-22 | 1984-04-10 | The United States Of America As Represented By The Department Of Health And Human Services | Simian virus recombinant that directs the synthesis of hepatitis B surface antigen |
| US4515714A (en) * | 1983-03-09 | 1985-05-07 | Juridicial Foundation, The Chemo-Semo-Sero-Therapeutic Research Institute | Method for purification of hepatitis B virus surface antigen |
-
1983
- 1983-10-05 JP JP58186187A patent/JPS6078999A/ja active Granted
-
1984
- 1984-10-01 US US06/656,315 patent/US4612283A/en not_active Expired - Lifetime
- 1984-10-04 EP EP84111917A patent/EP0138167B1/en not_active Expired
- 1984-10-04 DE DE8484111917T patent/DE3468623D1/de not_active Expired
- 1984-10-05 KR KR1019840006164A patent/KR910008643B1/ko not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6078999A (ja) | 1985-05-04 |
| KR910008643B1 (ko) | 1991-10-19 |
| EP0138167B1 (en) | 1988-01-13 |
| KR850003166A (ko) | 1985-06-13 |
| DE3468623D1 (en) | 1988-02-18 |
| EP0138167A1 (en) | 1985-04-24 |
| US4612283A (en) | 1986-09-16 |
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