JP6301906B2 - 5’toputrを含む人工核酸分子 - Google Patents
5’toputrを含む人工核酸分子 Download PDFInfo
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- JP6301906B2 JP6301906B2 JP2015502143A JP2015502143A JP6301906B2 JP 6301906 B2 JP6301906 B2 JP 6301906B2 JP 2015502143 A JP2015502143 A JP 2015502143A JP 2015502143 A JP2015502143 A JP 2015502143A JP 6301906 B2 JP6301906 B2 JP 6301906B2
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- PBKWZFANFUTEPS-CWUSWOHSSA-N transportan Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(N)=O)[C@@H](C)CC)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)CN)[C@@H](C)O)C1=CC=C(O)C=C1 PBKWZFANFUTEPS-CWUSWOHSSA-N 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- DJJCXFVJDGTHFX-XVFCMESISA-N uridine 5'-monophosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-XVFCMESISA-N 0.000 description 1
- 229960005502 α-amanitin Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
- A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
- C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/10—Vectors comprising a special translation-regulating system regulates levels of translation
- C12N2840/105—Vectors comprising a special translation-regulating system regulates levels of translation enhancing translation
Description
a. TOP遺伝子の5’UTRに由来するか又はTOP遺伝子の5’UTRの変異体に由来する核酸配列を含むか又はからなる少なくとも1つの5’−非翻訳領域エレメント(5’UTRエレメント)と、
b. 少なくとも1つのオープンリーディングフレーム(ORF)と
とを含む人工核酸分子に関する。
c. 脊索動物の遺伝子、好ましくは脊椎動物の遺伝子、より好ましくは哺乳類の遺伝子、最も好ましくはヒトの遺伝子の3’UTRに由来するか、又は脊索動物の遺伝子、好ましくは脊椎動物の遺伝子、より好ましくは哺乳類の遺伝子、最も好ましくはヒトの遺伝子の3’UTRの変異体に由来する核酸配列を含むか又はからなる少なくとも1つの3’UTRエレメント
を更に含む。
PPAx=(E1x−E0x)+(E2x−E0x)+E0x
E0は、コンストラクトE0(UTRを有しない)について得られるタンパク質の量であり、E1は、コンストラクトE1について得られるタンパク質の量であり、E2は、コンストラクトE2について得られるタンパク質の量であり、xは、発現開始後の時点である。E1E2x=PPAxである場合、タンパク質産生増加効果は、相加的であり、E1E2x>PPAxである場合、本発明における意味で相乗的である。ここで、E1E2xは、時点xにおいてコンストラクトE1E2から得られるタンパク質の量である。好ましくは、E1E2は、発現開始の24時間後、48時間後、又は72時間後等の発現開始後の所与の時点において、PPAの少なくとも1.0倍、より好ましくは少なくとも1.1倍、より好ましくは少なくとも1.3倍、より好ましくは少なくとも1.5倍、更により好ましくは少なくとも1.75倍である。
5’−キャップ−5’UTRエレメント−ORF−3’UTRエレメント−ヒストンステムループ−ポリ(A)配列
5’−キャップ−5’UTRエレメント−ORF−3’UTRエレメント−ポリ(A)配列−ヒストンステムループ
5’−キャップ−5’UTRエレメント−ORF−IRES−ORF−3’UTRエレメント−ヒストンステムループ−ポリ(A)配列
5’−キャップ−5’UTRエレメント−ORF−IRES−ORF−3’UTRエレメント−ポリ(A)配列−ヒストンステムループ
5’−キャップ−5’UTRエレメント−ORF−3’UTRエレメント−ポリ(A)配列−ポリ(C)配列
5’−キャップ−5’UTRエレメント−ORF−3’UTRエレメント−ポリ(A)配列−ポリ(C)配列−ヒストンステムループ
5’−キャップ−5’UTRエレメント−ORF−IRES−ORF−3’UTRエレメント−ヒストンステムループ−ポリ(A)配列−ポリ(C)配列。
Proのコドンは、CC(U/T)又はCCAからCCC又はCCGに改変してよい;
Argのコドンは、CG(U/T)又はCGA又はAGA又はAGGからCGC又はCGGに改変してよい;
Alaのコドンは、GC(U/T)又はGCAからGCC又はGCGに改変してよい;
Glyのコドンは、GG(U/T)又はGGAからGGC又はGGGに改変してよい。
Pheのコドンは、(U/T)(U/T)(U/T)から(U/T)(U/T)Cに改変してよい;
Leuのコドンは、(U/T)(U/T)A、(U/T)(U/T)G、C(U/T)(U/T)又はC(U/T)AからC(U/T)C又はC(U/T)Gに改変してよい;
Serのコドンは、(U/T)C(U/T)又は(U/T)CA又はAG(U/T)から(U/T)CC、(U/T)CG又はAGCに改変してよい;
Tyrのコドンは、(U/T)A(U/T)から(U/T)ACに改変してよい;
Cysのコドンは、(U/T)G(U/T)から(U/T)GCに改変してよい;
Hisのコドンは、CA(U/T)からCACに改変してよい;
Glnのコドンは、CAAからCAGに改変してよい;
Ileのコドンは、A(U/T)(U/T)又はA(U/T)AからA(U/T)Cに改変してよい;
Thrのコドンは、AC(U/T)又はACAからACC又はACGに改変してよい;
Asnのコドンは、AA(U/T)からAACに改変してよい;
Lysのコドンは、AAAからAAGに改変してよい;
Valのコドンは、G(U/T)(U/T)又はG(U/T)AからG(U/T)C又はG(U/T)Gに改変してよい;
Aspのコドンは、GA(U/T)からGACに改変してよい;
Gluのコドンは、GAAからGAGに改変してよい;
終止コドン(U/T)AAは、(U/T)AG又は(U/T)GAに改変してよい。
a. TOP遺伝子の5’UTRに由来するか又はTOP遺伝子の5’UTRの変異体に由来する核酸配列を含むか又はからなる少なくとも1つの5’非翻訳領域エレメント(5’UTRエレメント)と、
b. 少なくとも1つのオープンリーディングフレーム(ORF)及び/又はクローニングサイトと、
c. 任意で、脊索動物の遺伝子、好ましくは脊椎動物の遺伝子、より好ましくは哺乳類の遺伝子、最も好ましくはヒトの遺伝子の3’UTR、又は脊索動物の遺伝子、好ましくは脊椎動物の遺伝子、より好ましくは哺乳類の遺伝子、最も好ましくはヒトの遺伝子の3’UTRの変異体に由来する核酸配列を含むか又はからなる少なくとも1つの3’UTRエレメントと
を含むベクターを提供する。
{(Arg)l;(Lys)m;(His)n;(Orn)o;(Xaa)x};式(I)
(式中、l+m+n+o+xは、3〜100であり、l、m、n、又はoは、互いに独立して、0、1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21〜30、31〜40、41〜50、51〜60、61〜70、71〜80、81〜90、及び91〜100から選択される任意の数であるが、但し、Arg(アルギニン)、Lys(リジン)、His(ヒスチジン)及びOrn(オルニチン)の総含量は、オリゴペプチドの全アミノ酸の少なくとも10%であり;Xaaは、Arg、Lys、His又はOrnを除くネイティブ(即ち、自然界に存在する)又は非ネイティブなアミノ酸から選択される任意のアミノ酸であり;xは、0、1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21〜30、31〜40、41〜50、51〜60、61〜70、71〜80、81〜90から選択される任意の数であるが、但し、Xaaの総含量は、オリゴペプチドの全アミノ酸の90%を超えない)。アミノ酸Arg、Lys、His、Orn、及びXaaはいずれも、ペプチドの任意の位置に位置してよい。これに関連して、7アミノ酸〜30アミノ酸のカチオン性ペプチド又はタンパク質が特に好ましい。
{(Arg)l;(Lys)m;(His)n;(Orn)o;(Xaa’)x(Cys)y} 部分式(Ia)
(式中、(Arg)l;(Lys)m;(His)n;(Orn)o;及びxは、本明細書に定義する通りであり、Xaa’は、Arg、Lys、His、Orn、又はCysを除くネイティブ(即ち、自然界に存在する)又は非ネイティブなアミノ酸から選択される任意のアミノ酸であり、yは、0、1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21〜30、31〜40、41〜50、51〜60、61〜70、71〜80、81〜90から選択される任意の数であるが、但し、Arg(アルギニン)、Lys(リジン)、His(ヒスチジン)及びOrn(オルニチン)の総含量は、オリゴペプチドの全アミノ酸の少なくとも10%である)。更に、カチオン性又はポリカチオン性のペプチドは、部分式(Ib)から選択してよい:
Cys1{(Arg)l;(Lys)m;(His)n;(Orn)o;(Xaa)x}Cys2 部分式(Ib)
(式中、経験式{(Arg)l;(Lys)m;(His)n;(Orn)o;(Xaa)x}(式(III))は、本明細書に定義する通りであり、(半経験)式(III)に係るアミノ酸配列のコアを形成し、Cys1及びCys2は、(Arg)l;(Lys)m;(His)n;(Orn)o;(Xaa)xに近接するか又は末端に存在するシステインである)。
配列番号1,364 PpLuc(GC)−A64N64
配列番号1,365 PpLuc(GC)−アルブミン7−A64N64
配列番号1,366 RPL32−PpLuc(GC)−A64N64
配列番号1,367 RPL32−PpLuc(GC)−アルブミン7−A64N64
配列番号1,368 5’末端オリゴピリミジン領域を有しないヒトリボソームタンパク質ラージ32の5’UTR
配列番号1,369 ヒトアルブミン3’UTR
配列番号1,370 ヒトヘモグロビン、アルファ1(HBA1)の3’UTR
配列番号1,371 ヒトヘモグロビン、アルファ2(HBA2)の3’UTR
配列番号1,372 ヒトヘモグロビン、ベータ(HBB)の3’UTR
配列番号1,373 ヒトチロシンヒドロキシラーゼ(TH)の3’UTR
配列番号1,374 ヒトアラキドン酸15−リポキシゲナーゼ(ALOX15)の3’UTR
配列番号1,375 ヒトコラーゲンI型アルファ1(COL1A1)の3’UTR
配列番号1,376 アルブミン7の3’UTR
配列番号1,377 ヒトアルブミンの3’UTR+ポリ(A)配列
配列番号1,378 ヒトアルブミンの3’UTR断片1
配列番号1,379 ヒトアルブミンの3’UTR断片2
配列番号1,380 ヒトアルブミンの3’UTR断片3
配列番号1,381 ヒトアルブミンの3’UTR断片4
配列番号1,382 ヒトアルブミンの3’UTR断片5
配列番号1,383 ヒトアルブミンの3’UTR断片6
配列番号1,384 ヒトアルブミンの3’UTR断片7
配列番号1,385 ヒトアルブミンの3’UTR断片8
配列番号1,386 ヒトアルブミンの3’UTR断片9
配列番号1,387 ヒトアルブミンの3’UTR断片10
配列番号1,388 ヒトアルブミンの3’UTR断片11
配列番号1,389 ヒトアルブミンの3’UTR断片12
配列番号1,390 ヒトアルブミンの3’UTR断片13
配列番号1,391 アルブミン7の3’UTR−ポリ(A)配列−ポリ(C)配列−HL
配列番号1,392 アルブミン7の3’UTR−ポリ(A)配列−ポリ(C)配列
配列番号1,393 α−グロビン遺伝子の3’UTRの中央のα複合体結合部分
配列番号1,394 ヒストンステムループ
配列番号1,396 RPL35−PpLuc(GC)−アルブミン7−A64N64
配列番号1,397 RPL21−PpLuc(GC)−アルブミン7−A64N64
配列番号1,398 ATP5A1−PpLuc(GC)−アルブミン7−A64N64
配列番号1,399 HSD17B4−PpLuc(GC)−アルブミン7−A64N64
配列番号1,400 AIG1−PpLuc(GC)−アルブミン7−A64N64
配列番号1,401 COX6C−PpLuc(GC)−アルブミン7−A64N64
配列番号1,402 ASAH1−PpLuc(GC)−アルブミン7−A64N64
配列番号1,403 mRPL21−PpLuc(GC)−アルブミン7−A64N64
配列番号1,404 mRPL35A−PpLuc(GC)−アルブミン7−A64N64
配列番号1,405 RPL35−PpLuc(GC)−A64N64
配列番号1,406 RPL21−PpLuc(GC)−A64N64
配列番号1,407 ATP5A1−PpLuc(GC)−A64N64
配列番号1,408 HSD17B4−PpLuc(GC)−A64N64
配列番号1,409 AIG1−PpLuc(GC)−A64N64
配列番号1,410 COX6C−PpLuc(GC)−A64N64
配列番号1,411 ASAH1−PpLuc(GC)−A64N64
配列番号1,412 5’末端オリゴピリミジン領域を有しないヒトリボソームタンパク質ラージ35(RPL35)の5’UTR
配列番号1,413 5’末端オリゴピリミジン領域を有しないヒトリボソームタンパク質ラージ21(RPL21)の5’UTR
配列番号1,414 5’末端オリゴピリミジン領域を有しないヒトATPシンターゼ、H+輸送、ミトコンドリアF1複合体アルファサブユニット1、心筋(ATP5A1)の5’UTR
配列番号1,415 5’末端オリゴピリミジン領域を有しないヒトヒドロキシステロイド(17−ベータ)デヒドロゲナーゼ4(HSD17B4)の5’UTR
配列番号1,416 5’末端オリゴピリミジン領域を有しないヒトアンドロゲン誘導1(AIG1)の5’UTR
配列番号1,417 5’末端オリゴピリミジン領域を有しないヒトシトクロームcオキシダーゼサブユニットVIc(COX6C)の5’UTR
配列番号1,418 5’末端オリゴピリミジン領域を有しないヒトN−アシルスフィンゴシンアミドヒドロラーゼ(酸性セラミダーゼ)1(ASAH1)の5’UTR
配列番号1,419 5’末端オリゴピリミジン領域を有しないマウスリボソームタンパク質ラージ21(mRPL21)の5’UTR
配列番号1,420 5’末端オリゴピリミジン領域を有しないマウスリボソームタンパク質ラージ35A(mRPL35A)の5’UTR
T7プロモーターに続いて、キタアメリカホタルルシフェラーゼをコードしているGCリッチ配列(ppLuc(GC))及びA64ポリ(A)配列を含むインビトロ転写用ベクターを構築した。インビトロ転写前にベクターを線形化するために用いられる制限酵素部位をポリ(A)配列に続いて配置した。インビトロ転写によってこのベクターから得られたmRNAを「ppLuc(GC)−A64N64」と命名する。
配列番号1,364(図1):PpLuc(GC)−A64N64
配列番号1,365(図2):PpLuc(GC)−アルブミン7−A64N64
配列番号1,366(図3):RPL32−PpLuc(GC)−A64N64
配列番号1,367(図4):RPL32−PpLuc(GC)−アルブミン7−A64N64
配列番号1,396(図6):RPL35−PpLuc(GC)−アルブミン7−A64N64
配列番号1,397(図7):RPL21−PpLuc(GC)−アルブミン7−A64N64
配列番号1,398(図8):ATP5A1−PpLuc(GC)−アルブミン7−A64N64
配列番号1,399(図9):HSD17B4−PpLuc(GC)−アルブミン7−A64N64
配列番号1,400(図10):AIG1−PpLuc(GC)−アルブミン7−A64N64
配列番号1,401(図11):COX6C−PpLuc(GC)−アルブミン7−A64N64
配列番号1,402(図12):ASAH1−PpLuc(GC)−アルブミン7−A64N64
配列番号1,403(図13):mRPL21−PpLuc(GC)−アルブミン7−A64N64
配列番号1,404(図14):mRPL35A−PpLuc(GC)−アルブミン7−A64N64
配列番号1,405(図15):RPL35−PpLuc(GC)−A64N64
配列番号1,406(図16):RPL21−PpLuc(GC)−A64N64
配列番号1,407(図17):ATP5A1−PpLuc(GC)−A64N64
配列番号1,408(図18):HSD17B4−PpLuc(GC)−A64N64
配列番号1,409(図19):AIG1−PpLuc(GC)−A64N64
配列番号1,410(図20):COX6C−PpLuc(GC)−A64N64
配列番号1,411(図21):ASAH1−PpLuc(GC)−A64N64
実施例1に係るDNAテンプレートを線形化し、T7−ポリメラーゼを用いてインビトロで転写させた。次いで、DNAテンプレートをDNase処理によって分解した。過剰のN7−メチル−グアノシン−5’−トリホスフェートー5’−グアノシンを転写反応物に添加することによって、5’−キャップ構造を含むmRNA転写産物を得た。このようにして得られたmRNAを精製し、水に再懸濁させた。
1ウェル当たり5×104個の細胞という密度になるようにヒト皮膚線維芽細胞(HDF)を24ウェルプレートに播種した。次の日、細胞をopti−MEMで洗浄し、次いで、opti−MEM中リポフェクタミン2000複合体化ppLucコードmRNA(1ウェル当たり50ng)でトランスフェクトした。コントロールとして、ppLucをコードしていないmRNAを別個にリポフェクトした。トランスフェクション効率を制御するために、ウミシイタケ(Renilla reniformis)ルシフェラーゼ(RrLuc)をコードしているmRNAをPpLuc mRNAと共にトランスフェクトした(1ウェル当たりRrLuc mRNA20ng)。トランスフェクション開始の90分間後、opti−MEMを培地に交換した。トランスフェクションの24時間後、48時間後、72時間後、培地を吸引し、リシスバッファ200μL(25mM Tris、pH7.5(HCl)、2mM EDTA、10% グリセロール、1% Triton X−100、2mM DTT、1mM PMSF)に細胞を溶解させた。ルシフェラーゼ活性を測定するまで溶解物を−20℃で保存した。
BioTek Synergy HTプレートリーダーによって相対光単位(RLU)としてルシフェラーゼ活性を測定した。PpLuc活性は、溶解物50μL及びルシフェリンバッファ200μL(75μM ルシフェリン、25mM グリシルグリシン、pH7.8(NaOH)、15mM MgSO4、2mM ATP)を用いて、15秒間の測定時間で測定した。RrLuc活性は、溶解物50μL及びセレンテラジンバッファ200μL(500mM NaClに調整したリン酸緩衝生理食塩水中40μMセレンテラジン)を用いて、15秒間の測定時間で測定した。
5.1 TOP5’UTRエレメントとアルブミン3’UTRエレメントとの組み合わせは、mRNAからのタンパク質発現を相乗的に増加させる
mRNAからのタンパク質発現に対するTOP5’UTRエレメントとアルブミン3’UTRエレメントとの組み合わせの効果について調べるために、様々なUTRを有するmRNAを合成した:TOP5’UTRエレメント及びアルブミン3’UTRエレメントのいずれも有しないmRNA、又はTOP5’UTRエレメント(RPL32)若しくはアルブミン3’UTRエレメント(アルブミン7)のいずれかを含むmRNA、又はTOP5’UTRエレメント及びアルブミン3’UTRエレメントの両方を含むmRNA。ルシフェラーゼをコードしているmRNA又はコントロールmRNAをヒト皮膚線維芽細胞(HDF)にトランスフェクトした。トランスフェクションの24時間後、48時間後、及び72時間後にルシフェラーゼレベルを測定した。コトランスフェクトしたRrLucのシグナルによるトランスフェクション効率に対して、PpLucシグナルを補正した(以下の表1及び図5を参照)。
mRNAからのタンパク質発現に対するTOP5’UTRエレメントの効果について調べるために、様々なTOP5’UTRエレメントを含むmRNAを合成した。更に、mRNAは、アルブミン7の3’UTRエレメントを含んでいた。ルシフェラーゼをコードしているmRNAをヒト皮膚線維芽細胞(HDF)にトランスフェクトした。トランスフェクションの24時間後、48時間後、及び72時間後にルシフェラーゼレベルを測定した(以下の表3及び図22を参照)。
mRNAからのタンパク質発現に対するTOP5’UTRエレメントとアルブミン3’UTRエレメントとの組み合わせの効果について調べるために、様々なUTRエレメントを有するmRNAを合成した:TOP5’UTRエレメント及びアルブミン3’UTRエレメントのいずれも有しないmRNA、アルブミン3’UTRエレメントを含むmRNA、様々なTOP5’UTRエレメントのうちの1つを含むmRNA、又は様々なTOP5’UTRエレメントのうちの1つとアルブミン3’UTRエレメントとの両方を含むmRNA。ルシフェラーゼをコードしているmRNAをヒト皮膚線維芽細胞(HDF)にトランスフェクトした。トランスフェクションの24時間後、48時間後、及び72時間後にルシフェラーゼレベルを測定した(図23〜30を参照)。ルシフェラーゼは、TOP5’UTRエレメントもアルブミン3’UTRエレメントも有しないmRNAから明らかに発現していた。5’UTRも3’UTRも有しないmRNAに比べて、アルブミン3’UTRエレメントは、ルシフェラーゼレベルを延長させ、一方、TOP5’UTRエレメントは、ルシフェラーゼレベルを増加させた。しかし、驚くべきことに、TOP5’UTRエレメントとアルブミン3’UTRエレメントとの組み合わせは、個々のエレメントのいずれかでみられたレベルを遥かに超えてルシフェラーゼレベルを更に強く増加させた。同一のmRNAにおいてTOP5’UTRエレメントとアルブミン3’UTRエレメントとを組み合わせたことによるルシフェラーゼレベルの増加の程度は、これらが相乗的に作用することを示す。
mRNAからのタンパク質発現に対するマウス遺伝子由来のTOP5’UTRエレメントの効果について調べるために、2つの異なるマウスTOP5’UTRエレメントを有するmRNAを合成した。更に、mRNAは、アルブミン7の3’UTRエレメントを含んでいた。ルシフェラーゼをコードしているmRNAをヒト皮膚線維芽細胞(HDF)にトランスフェクトした。比較のために、ヒトRPL32のTOP5’UTRエレメントを含有するmRNAをトランスフェクトした。トランスフェクションの24時間後、48時間後、及び72時間後にルシフェラーゼレベルを測定した(以下の表5及び図30を参照)。
Claims (38)
- a. 少なくとも1つの5’−非翻訳領域エレメント(5’UTRエレメント)と、
b. 少なくとも1つのオープンリーディングフレーム(ORF)と、
c. 少なくとも1つの3’UTRエレメントと、
を含み、
前記5’UTRエレメントが、配列番号1,368若しくは1,412〜1,420に係る核酸配列又は対応するRNA配列に対して少なくとも95%の同一性を有する核酸配列を含むか又はからなる、或いは、前記5’UTRエレメントが、配列番号1,368若しくは1,412〜1,420に係る核酸配列又は対応するRNA配列に対して少なくとも95%の同一性を有する核酸配列の断片を含むか又はからなり、かつ、前記配列番号1,368若しくは1,412〜1,420に比べて、mRNAの翻訳効率を実質的に減じるものではなく、
前記5’UTRエレメントが、機能的5’TOPモチーフを含有せず、
前記3’UTRエレメントが、アルブミン遺伝子の3’UTRに由来するか又はアルブミン遺伝子の3’UTRの変異体に由来する核酸配列を含むか又はからなり、
前記3’UTRの変異体が、前記アルブミン遺伝子の3’UTRと少なくとも95%同一であり、
前記3’UTRエレメントが、前記アルブミン遺伝子の3’UTRに比べて、mRNAの翻訳効率を実質的に減じるものではないことを特徴とする人工核酸分子。 - 前記3’UTRエレメントが、脊椎動物のアルブミン遺伝子の3’UTR又はその変異体に由来する核酸配列を含むか又はからなる請求項1に記載の人工核酸分子。
- 前記3’UTRエレメントが、少なくとも75ヌクレオチドの長さを有する請求項1から2のいずれかに記載の人工核酸分子。
- 5’UTRエレメントとオープンリーディングフレームとが、異種である請求項1から3のいずれかに記載の人工核酸分子。
- 5’UTRエレメントが、人工核酸分子からのタンパク質産生を増加させるのに好適である請求項1から4のいずれかに記載の人工核酸分子。
- 5’UTRエレメントが、その5’末端において、TOP遺伝子の5’UTRのTOPモチーフの下流の位置1、2、3、4、5、6、7、8、9、又は10に位置するヌクレオチドで始まる請求項1から5のいずれかに記載の人工核酸分子。
- 5’UTRエレメントが、その3’末端において、前記5’UTRが由来する遺伝子又はmRNAのオープンリーディングフレームの開始コドンの上流の位置1、2、3、4、5、6、7、8、9、又は10に位置するヌクレオチドで終わる請求項1から6のいずれかに記載の人工核酸分子。
- 前記3’UTRエレメントと前記5’UTRエレメントとが、少なくとも相加的に作用して、人工核酸分子からのタンパク質産生を増加させる請求項1から7のいずれかに記載の人工核酸分子。
- d. ポリ(A)配列及びポリアデニル化シグナルの少なくともいずれか
を更に含む請求項1から8のいずれかに記載の人工核酸分子。 - ポリアデニル化シグナルが、3’UTRエレメント内に位置する請求項9に記載の人工核酸分子。
- ポリアデニル化シグナルが、コンセンサス配列NN(U/T)ANA(Nは、A又はUである)を含む請求項9から10のいずれかに記載の人工核酸分子。
- ポリアデニル化シグナルが、3’UTRの3’末端の50ヌクレオチド未満上流に位置する請求項9から11のいずれかに記載の人工核酸分子。
- ポリ(A)配列が、20アデニンヌクレオチド〜300アデニンヌクレオチドの長さを有する請求項9から12のいずれかに記載の人工核酸分子。
- 断片が、前記断片が由来する完全長配列の少なくとも90%を表す、前記完全長配列における連続する一続きのヌクレオチドに対応する連続する一続きのヌクレオチドからなる請求項1から13のいずれかに記載の人工核酸分子。
- 前記3’UTRエレメントが、配列番号1,369及び1,376から選択される核酸配列又は対応するRNA配列に対して少なくとも95%の同一性を有する核酸配列を含むか又はからなる、或いは、前記3’UTRエレメントが、配列番号1,369及び1,376から選択される核酸配列又は対応するRNA配列に対して少なくとも95%の同一性を有する核酸配列の断片を含むか又はからなり、かつ、前記配列番号1,369及び1,376に比べて、mRNAの翻訳効率を実質的に減じるものではない請求項1から14のいずれかに記載の人工核酸分子。
- 断片が、前記断片が由来する完全長配列の少なくとも90%を表す、前記完全長配列における連続する一続きのヌクレオチドに対応する連続する一続きのヌクレオチドからなる請求項15に記載の人工核酸分子。
- オープンリーディングフレームが、レポータータンパク質をコードしていない請求項1から16のいずれかに記載の人工核酸分子。
- 前記レポータータンパク質が、GFPタンパク質、ルシフェラーゼタンパク質、グロビンタンパク質、ヒト成長ホルモン、又はヒトのアルブミンのいずれかである請求項17に記載の人工核酸分子。
- 5’−キャップ構造、ポリ(C)配列、及び/又はIRESモチーフを更に含む請求項1から18のいずれかに記載の人工核酸分子。
- プロモーター含有配列を更に含む請求項1から19のいずれかに記載の人工核酸分子。
- 人工核酸分子が、少なくとも部分的にG/C改変されている請求項1から20のいずれかに記載の人工核酸分子。
- オープンリーディングフレームのG/C含量が、野生型オープンリーディングフレームに比べて増加している請求項1から21のいずれかに記載の人工核酸分子。
- オープンリーディングフレームが、コドンが最適化されている領域を含む請求項1から22のいずれかに記載の人工核酸分子。
- RNAの分子である請求項1から23のいずれかに記載の人工核酸分子。
- mRNAの分子である請求項1から24のいずれかに記載の人工核酸分子。
- a. 少なくとも1つの5’−非翻訳領域エレメント(5’UTRエレメント)と、
b. 少なくとも1つのオープンリーディングフレーム(ORF)及び少なくとも1つのクローニングサイトの少なくともいずれかと、
c. 少なくとも1つの3’UTRエレメントと、
を含み、
前記5’UTRエレメントが、配列番号1,368若しくは1,412〜1,420に係る核酸配列又は対応するRNA配列に対して少なくとも95%の同一性を有する核酸配列を含むか又はからなる、或いは、前記5’UTRエレメントが、配列番号1,368若しくは1,412〜1,420に係る核酸配列又は対応するRNA配列に対して少なくとも95%の同一性を有する核酸配列の断片を含むか又はからなり、かつ、前記配列番号1,368若しくは1,412〜1,420に比べて、mRNAの翻訳効率を実質的に減じるものではなく、
前記5’UTRエレメントが、機能的5’TOPモチーフを含有せず、
前記3’UTRエレメントが、アルブミン遺伝子の3’UTRに由来するか又はアルブミン遺伝子の3’UTRの変異体に由来する核酸配列を含むか又はからなり、
前記3’UTRの変異体が、前記アルブミン遺伝子の3’UTRと少なくとも95%同一であり、
前記3’UTRエレメントが、前記アルブミン遺伝子の3’UTRに比べて、mRNAの翻訳効率を実質的に減じるものではないことを特徴とするベクター。 - 5’UTRエレメントとオープンリーディングフレームとが、異種である請求項26に記載のベクター。
- RNAベクター又はDNAベクターである請求項26から27のいずれかに記載のベクター。
- プラスミドベクター又はウイルスベクターである請求項26から28のいずれかに記載のベクター。
- 請求項1から25のいずれかに記載の人工核酸分子を含むか又はコードしている請求項26から29のいずれかに記載のベクター。
- 環状分子である請求項26から30のいずれかに記載のベクター。
- 5’→3’方向において、コード鎖のORF、ポリ(A)配列、又は3’UTRエレメントに続いて環状ベクター分子を線形化するための制限酵素部位が配置されている請求項31に記載のベクター。
- 請求項1から25のいずれかに記載の人工核酸分子又は請求項26から32のいずれかに記載のベクターを含むことを特徴とする細胞。
- 哺乳類細胞である請求項33に記載の細胞。
- 請求項1から25のいずれかに記載の人工核酸分子、請求項26から32のいずれかに記載のベクター、又は請求項33から34のいずれかに記載の細胞を含むことを特徴とする医薬組成物。
- 1以上の薬学的に許容できる希釈剤、賦形剤、及び1以上のアジュバントの少なくともいずれかを更に含む請求項35に記載の医薬組成物。
- 医薬として使用するための請求項1から25のいずれかに記載の人工核酸分子、請求項26から32のいずれかに記載のベクター、請求項33から34のいずれかに記載の細胞、又は請求項35から36のいずれかに記載の医薬組成物。
- ワクチンとして又は遺伝子治療において使用するための請求項1から25のいずれかに記載の人工核酸分子、請求項26から32のいずれかに記載のベクター、請求項33から34のいずれかに記載の細胞、又は請求項35から36のいずれかに記載の医薬組成物。
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CA2866945A1 (en) | 2013-10-03 |
CN104321432B (zh) | 2018-08-10 |
WO2013143700A2 (en) | 2013-10-03 |
WO2013143700A3 (en) | 2013-12-27 |
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