JP5541972B2 - 走査型共焦点顕微鏡 - Google Patents

走査型共焦点顕微鏡 Download PDF

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Publication number
JP5541972B2
JP5541972B2 JP2010131772A JP2010131772A JP5541972B2 JP 5541972 B2 JP5541972 B2 JP 5541972B2 JP 2010131772 A JP2010131772 A JP 2010131772A JP 2010131772 A JP2010131772 A JP 2010131772A JP 5541972 B2 JP5541972 B2 JP 5541972B2
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Japan
Prior art keywords
photodetector
confocal microscope
light
scanning
mirror
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Japanese (ja)
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JP2011257583A5 (enExample
JP2011257583A (ja
Inventor
侑也 宮薗
大介 西脇
林　　真市
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Olympus Corp
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Olympus Corp
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Priority to JP2010131772A priority Critical patent/JP5541972B2/ja
Priority to EP11004598.6A priority patent/EP2395380B1/en
Priority to US13/153,559 priority patent/US8530824B2/en
Publication of JP2011257583A publication Critical patent/JP2011257583A/ja
Publication of JP2011257583A5 publication Critical patent/JP2011257583A5/ja
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B5/00—Optical elements other than lenses
    • G02B5/32—Holograms used as optical elements

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Microscoopes, Condenser (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Diffracting Gratings Or Hologram Optical Elements (AREA)
JP2010131772A 2010-06-09 2010-06-09 走査型共焦点顕微鏡 Active JP5541972B2 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2010131772A JP5541972B2 (ja) 2010-06-09 2010-06-09 走査型共焦点顕微鏡
EP11004598.6A EP2395380B1 (en) 2010-06-09 2011-06-06 Scanning microscope
US13/153,559 US8530824B2 (en) 2010-06-09 2011-06-06 Scanning microscope

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2010131772A JP5541972B2 (ja) 2010-06-09 2010-06-09 走査型共焦点顕微鏡

Publications (3)

Publication Number Publication Date
JP2011257583A JP2011257583A (ja) 2011-12-22
JP2011257583A5 JP2011257583A5 (enExample) 2013-06-20
JP5541972B2 true JP5541972B2 (ja) 2014-07-09

Family

ID=44509883

Family Applications (1)

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JP2010131772A Active JP5541972B2 (ja) 2010-06-09 2010-06-09 走査型共焦点顕微鏡

Country Status (3)

Country Link
US (1) US8530824B2 (enExample)
EP (1) EP2395380B1 (enExample)
JP (1) JP5541972B2 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5541972B2 (ja) * 2010-06-09 2014-07-09 オリンパス株式会社 走査型共焦点顕微鏡
JP5945400B2 (ja) * 2011-11-18 2016-07-05 オリンパス株式会社 検出光学系および走査型顕微鏡
JP6120611B2 (ja) * 2013-02-27 2017-04-26 日立マクセル株式会社 ビーム走査型表示装置
CN105352923B (zh) * 2014-10-21 2018-08-03 清华大学 一种快速宽视场体全息荧光显微成像系统
WO2016061710A1 (zh) * 2014-10-21 2016-04-28 清华大学 一种快速宽视场体全息荧光显微成像系统
JP2017219400A (ja) * 2016-06-07 2017-12-14 オリンパス株式会社 レーザ顕微鏡
CN111879247B (zh) * 2020-08-03 2022-05-20 海伯森技术（深圳）有限公司 一种测量轴孔规格的装置
CN119173798A (zh) * 2022-05-16 2024-12-20 高露洁-棕榄公司 用于使用镜面针孔阵列对多于一个样品平面同时采样的技术
CN115201954B (zh) * 2022-09-07 2022-12-06 杭州拓致光电科技有限公司 一种低通道串扰的复用体光栅角度放大器及其制备方法

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US4752130A (en) 1986-10-24 1988-06-21 The University Of Rochester Optical systems utilizing a volume transmission diffraction element to provide wavelength tuning
DE4330347C2 (de) * 1993-09-08 1998-04-09 Leica Lasertechnik Verwendung einer Vorrichtung zur Selektion und Detektion mindestens zweier Spektralbereiche eines Lichtstrahls
JP3106979B2 (ja) * 1996-10-31 2000-11-06 安藤電気株式会社 光スペクトル測定装置
DE19902625A1 (de) 1998-01-28 1999-09-30 Leica Microsystems Vorrichtung zur gleichzeitigen Detektion mehrerer Spektralbereiche eines Lichtstrahls
US6831781B2 (en) 1998-02-26 2004-12-14 The General Hospital Corporation Confocal microscopy with multi-spectral encoding and system and apparatus for spectroscopically encoded confocal microscopy
GB9825267D0 (en) 1998-11-19 1999-01-13 Medical Res Council Scanning confocal optical microscope system
DE10006800A1 (de) 2000-02-15 2001-08-16 Leica Microsystems Vorrichtung zur Selektion und Detektion mindestens eines Spektralbereichs eines spektral aufgefächerten Lichtstrahls
DE10038049A1 (de) * 2000-08-02 2002-02-14 Leica Microsystems Optische Anordnung zur Selektion und Detektion des Spektalbereichs eines Lichtstrahls
JP4761012B2 (ja) * 2000-08-11 2011-08-31 大日本印刷株式会社 Ｄｍｄを用いたカラー投影画像表示装置
US6583873B1 (en) 2000-09-25 2003-06-24 The Carnegie Institution Of Washington Optical devices having a wavelength-tunable dispersion assembly that has a volume dispersive diffraction grating
JP2002236257A (ja) * 2001-02-13 2002-08-23 Dainippon Printing Co Ltd マルチカラー共焦点顕微鏡
DE10156695B4 (de) 2001-11-17 2004-07-15 Leica Microsystems Heidelberg Gmbh Scanmikroskop, Verfahren zur Scanmikroskopie und Bandpassfilter
JP3576538B2 (ja) * 2002-06-11 2004-10-13 独立行政法人理化学研究所 グリズム
JP4416522B2 (ja) * 2004-01-26 2010-02-17 キヤノン株式会社 分光器
JP4633386B2 (ja) * 2004-05-25 2011-02-16 オリンパス株式会社 走査型レーザ顕微鏡及びそれを用いたデータ取得方法
JP4804727B2 (ja) 2004-06-24 2011-11-02 オリンパス株式会社 光走査型共焦点顕微鏡
JP4522174B2 (ja) * 2004-07-14 2010-08-11 富士通株式会社 光測定装置
EP1784680B1 (en) * 2004-08-27 2011-07-20 Koninklijke Philips Electronics N.V. Alignment system for spectroscopic analysis
DE102004049770A1 (de) * 2004-10-12 2006-04-13 Leica Microsystems Cms Gmbh Vorrichtung zur Selektion und Detektion mindestens zweier Spektralbereiche eines Lichtstrahls
JP4645173B2 (ja) 2004-11-26 2011-03-09 株式会社ニコン 分光器、及びこれを備えている顕微分光装置
JP4727981B2 (ja) 2004-12-22 2011-07-20 独立行政法人理化学研究所 回折格子装置
JP4539342B2 (ja) * 2005-01-19 2010-09-08 株式会社ニコン 解析装置、顕微鏡、および、解析プログラム
KR20080066705A (ko) * 2005-09-29 2008-07-16 더 제너럴 하스피탈 코포레이션 점진적으로 증가하는 분해능을 이용하여 하나 이상의 생물학적 샘플을 관찰 및 분석하기 위한 방법 및 장치
US7439483B2 (en) * 2006-04-17 2008-10-21 Korea Advanced Institute Of Science And Technology Real-time confocal microscope using the dispersion optics
JP4312775B2 (ja) * 2006-04-18 2009-08-12 韓国科学技術院 分散光学系を用いた実時間共焦点顕微鏡
DE102006034907A1 (de) 2006-07-28 2008-01-31 Carl Zeiss Microimaging Gmbh Laser-Scanning-Mikroskop
JP5536650B2 (ja) 2007-08-31 2014-07-02 ザ ジェネラル ホスピタル コーポレイション 自己干渉蛍光顕微鏡検査のためのシステムと方法、及び、それに関連するコンピュータがアクセス可能な媒体
WO2010008483A1 (en) 2008-06-25 2010-01-21 Bioptigen, Inc. Volume phase grating spectrometers and related methods and systems
US20100097693A1 (en) 2008-10-16 2010-04-22 Kazunori Koga Confocal microscope
JP2011197351A (ja) * 2010-03-19 2011-10-06 Olympus Corp 反射型回折格子、及び、それを用いた分光器及びパルス整形器
JP5541972B2 (ja) * 2010-06-09 2014-07-09 オリンパス株式会社 走査型共焦点顕微鏡
JP5775687B2 (ja) * 2010-12-17 2015-09-09 オリンパス株式会社 分光検出装置

Also Published As

Publication number Publication date
US8530824B2 (en) 2013-09-10
US20120032069A1 (en) 2012-02-09
EP2395380A1 (en) 2011-12-14
JP2011257583A (ja) 2011-12-22
EP2395380B1 (en) 2016-07-27

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