JP4729222B2 - 組換えクローニングにおける独自の選択性を有する複数の組換え部位の使用 - Google Patents

組換えクローニングにおける独自の選択性を有する複数の組換え部位の使用 Download PDF

Info

Publication number
JP4729222B2
JP4729222B2 JP2001544380A JP2001544380A JP4729222B2 JP 4729222 B2 JP4729222 B2 JP 4729222B2 JP 2001544380 A JP2001544380 A JP 2001544380A JP 2001544380 A JP2001544380 A JP 2001544380A JP 4729222 B2 JP4729222 B2 JP 4729222B2
Authority
JP
Japan
Prior art keywords
nucleic acid
recombination
sites
molecule
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2001544380A
Other languages
English (en)
Japanese (ja)
Other versions
JP2004500061A (ja
JP2004500061A5 (https=
Inventor
デイビッド チェオ，
マイケル エイ． ブラッシュ，
ギャリー エフ． テンプル，
ジェイムズ エル． ハートリー，
デヴォン アール． エヌ． ビルド，
Original Assignee
ライフ テクノロジーズ コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26865565&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP4729222(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ライフ テクノロジーズ コーポレーション filed Critical ライフ テクノロジーズ コーポレーション
Publication of JP2004500061A publication Critical patent/JP2004500061A/ja
Publication of JP2004500061A5 publication Critical patent/JP2004500061A5/ja
Application granted granted Critical
Publication of JP4729222B2 publication Critical patent/JP4729222B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2001544380A 1999-12-10 2000-12-11 組換えクローニングにおける独自の選択性を有する複数の組換え部位の使用 Expired - Lifetime JP4729222B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US16998399P 1999-12-10 1999-12-10
US60/169,983 1999-12-10
US18802000P 2000-03-09 2000-03-09
US60/188,020 2000-03-09
PCT/US2000/033546 WO2001042509A1 (en) 1999-12-10 2000-12-11 Use of multiple recombination sites with unique specificity in recombinational cloning

Publications (3)

Publication Number Publication Date
JP2004500061A JP2004500061A (ja) 2004-01-08
JP2004500061A5 JP2004500061A5 (https=) 2005-01-20
JP4729222B2 true JP4729222B2 (ja) 2011-07-20

Family

ID=26865565

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2001544380A Expired - Lifetime JP4729222B2 (ja) 1999-12-10 2000-12-11 組換えクローニングにおける独自の選択性を有する複数の組換え部位の使用

Country Status (11)

Country Link
US (1) US7393632B2 (https=)
EP (3) EP2210948A3 (https=)
JP (1) JP4729222B2 (https=)
CN (2) CN100587068C (https=)
AT (1) ATE391789T1 (https=)
AU (2) AU785483B2 (https=)
CA (1) CA2392753A1 (https=)
DE (1) DE60038572T2 (https=)
DK (1) DK1250453T3 (https=)
NZ (3) NZ530816A (https=)
WO (1) WO2001042509A1 (https=)

Families Citing this family (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6274369B1 (en) * 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
EP1025217B1 (en) * 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
EP1965213A3 (en) 1998-02-04 2009-07-15 Invitrogen Corporation Microarrays and uses therefor
AU766257C (en) 1998-05-07 2004-06-03 Universite Libre De Bruxelles Cytotoxin-based biological containment
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
EP2119788A1 (en) 1999-03-02 2009-11-18 Life Technologies Corporation Compositions and methods for use in recombinational cloning of nucleic acids
EP2210948A3 (en) 1999-12-10 2010-10-06 Life Technologies Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
ATE460472T1 (de) * 2000-12-08 2010-03-15 Life Technologies Corp Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen
WO2002053576A1 (en) 2001-01-05 2002-07-11 The General Hospital Corporation Viral delivery system for infectious transfer of large genomic dna inserts
US8470580B2 (en) 2001-02-23 2013-06-25 Universite Libre De Bruxelles Method for the selection of recombination clones comprising a sequence encoding an antidote protein to a toxic molecule
WO2002079409A2 (en) * 2001-03-29 2002-10-10 Rutgers, The University Of New Jersey Integrases for the insertion of heterologous nucleic acids into the plastid genome
EP1373527A1 (en) * 2001-04-06 2004-01-02 CropDesign N.V. The use of double and opposite recombination sites for the single step cloning of two dna segments
JP2004531259A (ja) * 2001-04-19 2004-10-14 インヴィトロジェン コーポレーション 核酸分子の組換えクローニングのための組成物および方法
EP1451344A4 (en) * 2001-05-21 2005-03-23 Invitrogen Corp COMPOSITIONS AND METHODS FOR USE IN ISOLATING NUCLEIC ACID MOLECULES
US20030059888A1 (en) * 2001-08-22 2003-03-27 Shengfeng Li Compositions and methods for generating antigen-binding units
US7985553B2 (en) * 2001-10-29 2011-07-26 Nathaniel Heintz Method for isolating cell type-specific mRNAs
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
US7056665B2 (en) * 2002-01-16 2006-06-06 Panomics, Inc. Screening methods involving the detection of short-lived proteins
US6955882B2 (en) * 2002-01-23 2005-10-18 Cropdesign N.V. Method for easy cloning and selection of chimeric DNA molecules
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
US20040005710A1 (en) * 2002-03-09 2004-01-08 Ho-Sun Son Method for producing recombinant viruses using site-specific recombination
US7319001B2 (en) * 2002-03-09 2008-01-15 Neurogenex Co., Ltd. High throughput system for producing recombinant viruses using site-specific recombination
US20050260585A1 (en) * 2002-03-19 2005-11-24 Cedric Szpirer Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms
US20040038304A1 (en) * 2002-03-28 2004-02-26 Gala Design, Inc. Antibody libraries
US20030228608A1 (en) * 2002-04-16 2003-12-11 Promega Corporation Method to enhance homologous recombination
US20040106566A1 (en) * 2002-05-17 2004-06-03 Shi-Lung Lin RNA-splicing and processing-directed gene silencing and the relative applications thereof
US7399586B2 (en) 2002-05-23 2008-07-15 Ceptyr, Inc. Modulation of biological signal transduction by RNA interference
US20040172667A1 (en) * 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US7527966B2 (en) * 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20040132133A1 (en) * 2002-07-08 2004-07-08 Invitrogen Corporation Methods and compositions for the production, identification and purification of fusion proteins
JP2005532829A (ja) * 2002-07-18 2005-11-04 インヴィトロジェン コーポレーション 組換え部位を含むウイルスベクター
WO2004022745A2 (en) 2002-09-03 2004-03-18 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
US9309518B2 (en) 2002-09-03 2016-04-12 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
AU2003270734A1 (en) * 2002-09-16 2004-04-30 University Of Southern California Rna-mediated gene modulation
CN1263860C (zh) * 2002-09-30 2006-07-12 华南农业大学 多基因载体的构建方法与应用
AU2003283995A1 (en) * 2002-09-30 2004-04-19 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
US7267984B2 (en) * 2002-10-31 2007-09-11 Rice University Recombination assembly of large DNA fragments
US7629120B2 (en) 2002-10-31 2009-12-08 Rice University Method for assembling PCR fragments of DNA
US7491539B2 (en) * 2002-12-05 2009-02-17 Boehringer Ingelheim Pharma Kg Sequence specific DNA recombination in eukaryotic cells
TW200502390A (en) * 2002-11-28 2005-01-16 Peter Droege Sequence specific DNA recombination in eukaryotic cells
US20040115642A1 (en) * 2002-12-12 2004-06-17 Changlin Fu Single-step methods for gene cloning
US7078234B2 (en) 2002-12-18 2006-07-18 Monsanto Technology Llc Maize embryo-specific promoter compositions and methods for use thereof
US20040253620A1 (en) * 2003-04-30 2004-12-16 Invitrogen Corporation Use of site specific recombination to prepare molecular markers
EP1881074B1 (en) 2003-05-05 2013-01-02 Monsanto Technology, LLC HRGP promoter constructs
US20060228800A1 (en) * 2003-05-15 2006-10-12 Shi-Lung Lin Novel Transgenic Methods Using intronic RNA
US20090203141A1 (en) * 2003-05-15 2009-08-13 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents
US9567591B2 (en) * 2003-05-15 2017-02-14 Mello Biotechnology, Inc. Generation of human embryonic stem-like cells using intronic RNA
WO2005028615A2 (en) * 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
WO2005014796A2 (en) * 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
WO2005033319A2 (en) 2003-10-02 2005-04-14 Monsanto Technology Llc Stacking crop improvement traits in transgenic plants
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
GB2408980B (en) * 2003-12-09 2006-06-07 Nat Biolog Standards Board Genetic reference materials
WO2005062881A2 (en) * 2003-12-24 2005-07-14 Transgenrx, Inc. Gene therapy using transposon-based vectors
US20050227309A1 (en) 2004-01-21 2005-10-13 Corry Schuyler B Optically-detectable enzyme substrates and their method of use
AR047598A1 (es) 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
US20090098611A1 (en) * 2004-02-27 2009-04-16 Wood David W Self-cleaving affinity tags and methods of use
US9944713B2 (en) 2004-11-24 2018-04-17 Medicinal Bioconvergence Research Center Antibody specific to the AIMP2-DX2
US8314290B2 (en) 2004-12-21 2012-11-20 Monsanto Technology Llc Temporal regulation of gene expression by MicroRNAs
US20070199095A1 (en) 2005-10-13 2007-08-23 Edwards Allen Methods for producing hybrid seed
WO2006073727A2 (en) 2004-12-21 2006-07-13 Monsanto Technology, Llc Recombinant dna constructs and methods for controlling gene expression
ATE552343T1 (de) * 2005-04-19 2012-04-15 Basf Plant Science Gmbh Endosperm-spezifische expression und/oder expression in keimenden embryos monokotyledoner pflanzen
WO2006135974A1 (en) * 2005-06-24 2006-12-28 Australian Centre For Plant Functional Genomics Pty Ltd Targeting vector
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
US8143474B2 (en) 2006-04-18 2012-03-27 Rutgers, The State University Of New Jersey Compositions and methods for increasing transgene expression in the plastids of higher plants
KR100762962B1 (ko) * 2006-05-04 2007-10-04 한국과학기술원 게놈정보 및 인실리코 분석을 이용한 배양배지의 제조방법
US20100196336A1 (en) 2006-05-23 2010-08-05 Dongsu Park Modified dendritic cells having enhanced survival and immunogenicity and related compositions and methods
KR100780324B1 (ko) 2006-07-28 2007-11-29 한국과학기술원 신규 순수 숙신산 생성 변이 미생물 및 이를 이용한 숙신산제조방법
WO2008027592A2 (en) 2006-08-31 2008-03-06 Monsanto Technology, Llc Phased small rnas
AU2007352460C1 (en) 2006-10-12 2013-05-23 Monsanto Technology, Llc Plant microRNAs and methods of use thereof
CA2675926A1 (en) 2007-02-16 2008-08-21 Basf Plant Science Gmbh Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants
WO2008103643A1 (en) 2007-02-20 2008-08-28 Monsanto Technology, Llc Invertebrate micrornas
EP2135945B1 (en) * 2007-03-14 2014-04-23 Japan Science and Technology Agency Method for of transcription-degradation dual regulation for protein by antibiotic
TWI382849B (zh) * 2007-10-29 2013-01-21 Mello Biotech Taiwan Co Ltd 產生藉由基因靜默效應以用於皮膚維護之化妝品的方法以及編碼基因靜默效應物之基因重組核苷酸
JP5670755B2 (ja) 2008-03-12 2015-02-18 ザ ロックフェラー ユニバーシティ 翻訳プロファイリング及び分子表現型解析のための方法及び組成物
US20090280103A1 (en) * 2008-04-04 2009-11-12 Martin Flueck Regulation of muscle repair
CA2729713C (en) 2008-07-01 2020-07-28 Monsanto Technology Llc Recombinant dna constructs and methods for modulating expression of a target gene
WO2010036976A2 (en) * 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
WO2010036979A2 (en) * 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of interferon
US9157097B2 (en) * 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
EP2417263B1 (en) * 2009-04-09 2015-09-23 ProteoVec Holding L.L.C. Production of proteins using transposon-based vectors
EP2449091A4 (en) 2009-07-02 2012-12-05 Verdezyne Inc BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID
AP3766A (en) 2009-07-09 2016-07-31 Du Pont Engineered microorganisms with enhanced fermentation activity
US8889394B2 (en) * 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
JPWO2012020574A1 (ja) * 2010-08-13 2013-10-28 静岡県公立大学法人 カビ類ポリケチド合成遺伝子の酵母での異種発現
US20120107815A1 (en) * 2010-10-28 2012-05-03 Arkray, Inc. Polymorphism Detection Probe, Polymorphism Detection Method, Evaluation of Drug Efficacy, and Polymorphism Detection Kit
US8695618B2 (en) 2010-12-22 2014-04-15 Carnegie Mellon University 3D chemical pattern control in 2D fluidics devices
KR102068107B1 (ko) * 2011-04-27 2020-01-20 아미리스 인코퍼레이티드 게놈 변형 방법
US8343752B2 (en) 2011-05-03 2013-01-01 Verdezyne, Inc. Biological methods for preparing adipic acid
US8728798B2 (en) 2011-05-03 2014-05-20 Verdezyne, Inc. Biological methods for preparing adipic acid
WO2013006733A2 (en) 2011-07-06 2013-01-10 Verdezyne, Inc. Biological methods for preparing a fatty dicarboxylic acid
CN102296059A (zh) * 2011-08-17 2011-12-28 石振宇 一种多段dna并行组装的方法
WO2013078433A1 (en) 2011-11-23 2013-05-30 University Of Hawaii Auto-processing domains for polypeptide expression
US9465519B2 (en) 2011-12-21 2016-10-11 Life Technologies Corporation Methods and systems for in silico experimental designing and performing a biological workflow
SG10201705057QA (en) 2012-12-19 2017-07-28 Verdezyne Inc Biological methods for preparing a fatty dicarboxylic acid
HUE052914T2 (hu) 2012-12-19 2021-05-28 Corvay Bioproducts Gmbh Biológiai eljárások zsírdikarbonsav elõállítására
WO2015024855A1 (en) 2013-08-19 2015-02-26 Syngulon Sa Controlled growth of microorganisms
US10513706B2 (en) 2014-04-09 2019-12-24 The Scripps Research Institute Import of unnatural or modified nucleoside triphosphates into cells via nucleic acid triphosphate transporters
US9745611B2 (en) * 2014-05-06 2017-08-29 Genewiz Inc. Methods and kits for identifying microorganisms in a sample
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
US10407676B2 (en) * 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2016154046A2 (en) 2015-03-20 2016-09-29 Verdezyne, Inc. Biological methods for preparing 3-hydroxypropionic acid
US11761007B2 (en) 2015-12-18 2023-09-19 The Scripps Research Institute Production of unnatural nucleotides using a CRISPR/Cas9 system
CN106397598B (zh) * 2016-02-23 2020-07-14 上海交通大学 多价多特异性抗体及免疫杂合蛋白的表达和制备方法
HUE060123T2 (hu) 2016-06-24 2023-01-28 Scripps Research Inst Új nukleozid-trifoszfát-transzporter és alkalmazásai
AU2018300069C1 (en) 2017-07-11 2025-11-20 Synthorx, Inc. Incorporation of unnatural nucleotides and methods thereof
CA3069697A1 (en) 2017-07-13 2019-01-17 Radici Chimica S.P.A. Biological methods for modifying cellular carbon flux
WO2019028419A1 (en) 2017-08-03 2019-02-07 Synthorx, Inc. CYTOKINE CONJUGATES FOR THE TREATMENT OF PROLIFERATIVE AND INFECTIOUS DISEASES
US11932672B2 (en) 2017-12-19 2024-03-19 Syngulon S.A. Fermentation process
KR102776907B1 (ko) 2017-12-29 2025-03-11 더 스크립스 리서치 인스티튜트 비천연 염기쌍 조성물 및 사용 방법
WO2020060948A1 (en) 2018-09-17 2020-03-26 Levadura Biotechnology, Inc. Production of cannabinoids in yeast using a fatty acid feedstock
KR20260039812A (ko) 2019-02-06 2026-03-20 신톡스, 인크. Il-2 콘쥬게이트 및 이의 사용 방법
EP3969594A1 (en) * 2019-05-15 2022-03-23 Deerland Probiotics & Enzymes A/S Inducible plasmid-self-destruction assisted recombination
CN120988042A (zh) 2019-06-14 2025-11-21 斯克利普斯研究所 用于在半合成生物体中复制、转录和翻译的试剂和方法
US20220307050A1 (en) * 2019-08-01 2022-09-29 The Regents Of The University Of California Non-viral transgenesis
CN118308427B (zh) * 2024-04-26 2024-09-27 深圳市库源生物科技有限公司 一种应用于多种构建体系的重组腺病毒载体质粒及其构建方法和应用

Family Cites Families (238)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4626505A (en) 1984-02-24 1986-12-02 E. I. Du Pont De Nemours And Company Selectable markers for yeast transformation
US4673640A (en) 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination
US4736866B1 (en) 1984-06-22 1988-04-12 Transgenic non-human mammals
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4808537A (en) 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
US4743546A (en) 1985-02-13 1988-05-10 Biotechnica International, Inc. Controlled gene excision
IT8520964U1 (it) 1985-03-01 1986-09-01 Alfa Romeo Auto Spa Contenitore per il liquido lavavetri di un autoveicolo
US5200333A (en) 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0208491B1 (en) 1985-07-03 1993-08-25 Genencor International, Inc. Hybrid polypeptides and process for their preparation
US4980286A (en) 1985-07-05 1990-12-25 Whitehead Institute For Biomedical Research In vivo introduction and expression of foreign genetic material in epithelial cells
US6121043A (en) 1985-09-06 2000-09-19 Syntro Corporation Recombinant herpesvirus of turkeys comprising a foreign DNA inserted into a non-essential region of the herpesvirus of turkeys genome
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5149639A (en) 1986-03-24 1992-09-22 Abbott Laboratories Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production
US4874748A (en) 1986-03-24 1989-10-17 Abbott Laboratories Cloning vectors for streptomyces and use thereof in macrolide antibiotic production
US4959217A (en) 1986-05-22 1990-09-25 Syntex (U.S.A.) Inc. Delayed/sustained release of macromolecules
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
WO1992006200A1 (en) 1990-09-28 1992-04-16 F. Hoffmann-La-Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4962022A (en) 1986-09-22 1990-10-09 Becton Dickinson And Company Storage and use of liposomes
JPS6427477A (en) * 1987-04-01 1989-01-30 Takeda Chemical Industries Ltd Dna and use thereof
ATE83008T1 (de) 1987-07-21 1992-12-15 Du Pont Merck Pharma Verfahren fuer die herstellung von in tierischen zellen stabilen und lebensfaehigen rekombinanten viralen vektoren.
US4960707A (en) 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
US5244797B1 (en) 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5063155A (en) 1988-03-28 1991-11-05 Eli Lilly And Company Method for producing 2"'-o-demethyltylosin
US5378618A (en) 1988-04-15 1995-01-03 E. I. Du Pont De Nemours And Company Vitro headful packaging system for cloning DNA fragments as large as 95kb
US5139942A (en) 1988-05-19 1992-08-18 New England Biolabs, Inc. Method for producing the nde i restriction endonuclease and methylase
US5098837A (en) 1988-06-07 1992-03-24 Eli Lilly And Company Macrolide biosynthetic genes for use in streptomyces and other organisms
US5498523A (en) 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5334375A (en) 1988-12-29 1994-08-02 Colgate Palmolive Company Antibacterial antiplaque oral composition
DE3901681A1 (de) 1989-01-21 1990-07-26 Behringwerke Ag Signalpeptid fuer die sekretion von peptiden in escherichia coli, verfahren zu seiner gewinnung und seine verwendung
CA2012983A1 (en) 1989-03-27 1990-09-27 Sydney Brenner Process for nucleic acid detection by binary amplification
US5102797A (en) 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5047342A (en) 1989-08-10 1991-09-10 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase
US5270179A (en) 1989-08-10 1993-12-14 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
WO1991002801A1 (en) 1989-08-22 1991-03-07 E.I. Du Pont De Nemours And Company An in vitro packaging system for cloning dna fragments of 95 kb
US5436146A (en) 1989-09-07 1995-07-25 The Trustees Of Princeton University Helper-free stocks of recombinant adeno-associated virus vectors
NO904633L (no) 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
CA2071943C (en) 1989-12-22 2007-04-24 Joan Tellefsen Odell Site-specific recombination of dna in plant cells
ATE201047T1 (de) 1990-01-08 2001-05-15 Stratagene Inc Neue wirtsorganismen zur klonierung
US5283173A (en) 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5082784A (en) 1990-03-20 1992-01-21 Life Technologies, Inc. Cloned kpni restriction-modification system
US5192675A (en) 1990-03-20 1993-03-09 Life Technologies, Inc. Cloned KpnI restriction-modification system
WO1991016427A1 (en) 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5098839A (en) 1990-05-10 1992-03-24 New England Biolabs, Inc. Type ii restriction endonuclease obtainable from pseudomonas alcaligenes and a process for producing the same
US5147800A (en) 1990-06-08 1992-09-15 Life Technologies, Inc. Host expressing ngoaiii restriction endonuclease and modification methylase from neisseria
EP0585287B1 (en) 1990-07-10 1999-10-13 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5179015A (en) 1990-07-23 1993-01-12 New England Biolabs, Inc. Heterospecific modification as a means to clone restriction genes
WO1992006180A1 (en) 1990-10-01 1992-04-16 University Of Connecticut Targeting viruses and cells for selective internalization by cells
US5227288A (en) 1990-10-01 1993-07-13 Blattner Frederick R DNA sequencing vector with reversible insert
DE553264T1 (de) 1990-10-05 1994-04-28 Wayne M Barnes Thermostabile dna polymerase.
US5202248A (en) 1990-11-02 1993-04-13 New England Biolabs, Inc. Method for cloning and producing the nco i restriction endonuclease and methylase
FR2670502B1 (fr) 1990-12-13 1994-09-16 Eurolysine Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.
US5286632A (en) 1991-01-09 1994-02-15 Jones Douglas H Method for in vivo recombination and mutagenesis
WO1993013663A1 (en) 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5328984A (en) 1991-03-04 1994-07-12 The United States As Represented By The Department Of Health & Human Services Recombinant chimeric proteins deliverable across cellular membranes into cytosol of target cells
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US5763170A (en) 1991-04-16 1998-06-09 Amersham International Plc Method for forming an array of biological particles
ES2154637T3 (es) 1991-05-14 2001-04-16 Univ Connecticut Aportacion de genes dirigidos que codifican proteinas inmunogenicas.
US5871907A (en) 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
US5858657A (en) 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
ES2149774T3 (es) 1991-06-05 2000-11-16 Univ Connecticut Aportacion dirigida de genes que codifican proteinas secretoras.
WO1992022650A1 (en) 1991-06-17 1992-12-23 Life Technologies, Inc. Recombination-facilitated multiplex analysis of dna fragments
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
AU3434393A (en) 1992-01-17 1993-08-03 Regents Of The University Of Michigan, The Targeted virus
EP0626999A4 (en) 1992-01-24 1997-04-09 Life Technologies Inc Modulation of enzyme activities in the -i(in vivo) cloning of dna.
ATE201236T1 (de) 1992-02-26 2001-06-15 Zeneca Mogen B V Zur ortsspezifischen rekomination fähige agrobakterium stämme
US5733743A (en) 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
DE69333823T2 (de) 1992-03-24 2006-05-04 Cambridge Antibody Technology Ltd., Melbourn Verfahren zur herstellung von gliedern von spezifischen bindungspaaren
JPH07505773A (ja) 1992-04-03 1995-06-29 ヤング　アレキサンダー　ティー 標的化されたウイルスベクターを用いた遺伝子治療
US5231021A (en) 1992-04-10 1993-07-27 Life Technologies, Inc. Cloning and expressing restriction endonucleases and modification methylases from xanthomonas
BE1006085A3 (fr) 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5733733A (en) 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5348886A (en) 1992-09-04 1994-09-20 Monsanto Company Method of producing recombinant eukaryotic viruses in bacteria
US6225121B1 (en) 1992-09-14 2001-05-01 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
SG41929A1 (en) 1992-09-25 1997-08-15 Commw Scient Ind Res Org Target binding polypeptide
KR950703640A (ko) 1992-10-09 1995-09-20 가일 케이,나우톤. 간 보존 세포(liver reserve cells)
WO1994009127A2 (en) 1992-10-16 1994-04-28 The Government Of The United States Of America As Represented By The Secretary, Department Of Healthand Human Services Supercoiled minicircle dna as as a unitary promoter vector
AU680459B2 (en) 1992-12-03 1997-07-31 Genzyme Corporation Gene therapy for cystic fibrosis
ATE199392T1 (de) 1992-12-04 2001-03-15 Medical Res Council Multivalente und multispezifische bindungsproteine, deren herstellung und verwendung
US5248605A (en) 1992-12-07 1993-09-28 Life Technologies, Inc. Cloning and expressing restriction endonucleases from haemophilus
US5334575A (en) 1992-12-17 1994-08-02 Eastman Kodak Company Dye-containing beads for laser-induced thermal dye transfer
US5312746A (en) 1993-01-08 1994-05-17 Life Technologies, Inc. Cloning and expressing restriction endonucleases and modification methylases from caryophanon
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
GB9302798D0 (en) 1993-02-12 1993-03-31 Medical Res Council Improvements in or relating to cloning dna
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
AU6359894A (en) 1993-03-05 1994-09-26 Crop Genetics International Corporation Agricultural-chemical-producing endosymbiotic microorganisms and method of preparing and using same
US5334526A (en) 1993-05-28 1994-08-02 Life Technologies, Inc. Cloning and expression of AluI restriction endonuclease
US6140087A (en) 1993-06-24 2000-10-31 Advec, Inc. Adenovirus vectors for gene therapy
US6120764A (en) 1993-06-24 2000-09-19 Advec, Inc. Adenoviruses for control of gene expression
US5919676A (en) 1993-06-24 1999-07-06 Advec, Inc. Adenoviral vector system comprising Cre-loxP recombination
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
EP0632054A1 (en) 1993-06-28 1995-01-04 European Molecular Biology Laboratory Regulation of site-specific recombination by site-specific recombinase/nuclear receptor fusion proteins
US5843744A (en) 1993-07-08 1998-12-01 Ecogen Inc. Bacillus thuringiensis Tn5401 proteins
US5441884A (en) 1993-07-08 1995-08-15 Ecogen Inc. Bacillus thuringiensis transposon TN5401
US5672491A (en) 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
US5712146A (en) 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
ES2164713T3 (es) 1993-09-20 2002-03-01 Univ Leland Stanford Junior Produccion recombinante de nuevos poliquetidos.
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5470740A (en) 1993-11-04 1995-11-28 Life Technologies, Inc. Cloned NsiI restriction-modification system
US5556772A (en) 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US5861273A (en) 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
US5470727A (en) 1993-12-21 1995-11-28 Celtrix Pharmaceuticals, Inc. Chromosomal expression of non-bacterial genes in bacterial cells
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
WO1995032225A1 (en) 1994-05-23 1995-11-30 The Salk Institute For Biological Studies Method for site-specific integration of nucleic acids and related products
US5723765A (en) 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US5589618A (en) 1994-09-01 1996-12-31 University Of Florida Materials and methods for increasing corn seed weight
US5534428A (en) 1994-09-06 1996-07-09 Life Technologies, Inc. Cloned Ssti/SacI restriction-modification system
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
ES2258265T3 (es) 1994-11-28 2006-08-16 Cell Genesys Inc. Vectores de replicacion con especificidad tisular.
US5998205A (en) 1994-11-28 1999-12-07 Genetic Therapy, Inc. Vectors for tissue-specific replication
FR2727689A1 (fr) 1994-12-01 1996-06-07 Transgene Sa Nouveau procede de preparation d'un vecteur viral
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US5981177A (en) 1995-01-25 1999-11-09 Demirjian; David C. Protein fusion method and constructs
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5814300A (en) * 1995-03-03 1998-09-29 Cephalon, Inc. Gene-targeted non-human mammals deficient in the SOD-1 gene
US6130364A (en) 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US5695971A (en) 1995-04-07 1997-12-09 Amresco Phage-cosmid hybrid vector, open cos DNA fragments, their method of use, and process of production
US5965796A (en) 1995-04-21 1999-10-12 University Of Georgia Research Foundation Inc. Metal resistance sequences and transgenic plants
US5877274A (en) 1995-06-02 1999-03-02 University Of British Columbia Antimicrobial cationic peptides
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
DK0937098T3 (da) 1995-06-07 2002-12-02 Invitrogen Corp Rekombinatorisk kloning under anvendelse af gensplejsede rekombinationssites
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
IL122859A0 (en) 1995-07-06 1998-08-16 Univ Leland Stanford Junior Cell-free synthesis of polyketides
WO1997006265A2 (en) 1995-08-07 1997-02-20 The Perkin-Elmer Corporation Recombinant clone selection system
US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
AUPN523995A0 (en) 1995-09-05 1995-09-28 Crc For Biopharmaceutical Research Pty Ltd Method for producing phage display vectors
EP0871775A4 (en) 1995-09-08 2002-07-31 Life Technologies Inc CLONED DNA POLYMERASES FROM THERMOTOGA AND MUTANTS THEREOF
US5770435A (en) 1995-11-02 1998-06-23 University Of Chicago Mutant E. coli strain with increased succinic acid production
CA2239366A1 (en) 1996-01-05 1997-07-17 Genetic Therapy, Inc. Recombinase-mediated generation of adenoviral vectors
US6228646B1 (en) 1996-03-07 2001-05-08 The Regents Of The University Of California Helper-free, totally defective adenovirus for gene therapy
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US5710248A (en) 1996-07-29 1998-01-20 University Of Iowa Research Foundation Peptide tag for immunodetection and immunopurification
JP2000516468A (ja) 1996-08-14 2000-12-12 ライフ テクノロジーズ，インコーポレイテッド 核酸増幅および配列決定のための安定な組成物
IL128736A0 (en) 1996-09-06 2000-01-31 Univ Pennsylvania Methods using cre-lox for production of recombinant adeno-associated viruses
US6025192A (en) 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
EP0941310A1 (en) * 1996-10-11 1999-09-15 THE TEXAS A&M UNIVERSITY SYSTEM Methods for the generation of primordial germ cells and transgenic animal species
US6066778A (en) 1996-11-06 2000-05-23 The Regents Of The University Of Michigan Transgenic mice expressing APC resistant factor V
EP0946286B1 (en) 1996-11-14 2003-03-19 Affymetrix, Inc. Chemical amplification for the synthesis of patterned arrays
US6110372A (en) 1996-12-17 2000-08-29 Perriello; Felix Anthony Bioremediation of petroleum pollutants with alkane-utilizing bacteria
US6033883A (en) 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
US6303301B1 (en) 1997-01-13 2001-10-16 Affymetrix, Inc. Expression monitoring for gene function identification
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
WO1998038326A1 (en) 1997-02-28 1998-09-03 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
EP2184289A1 (en) 1997-04-22 2010-05-12 Life Technologies Corporation Methods for the production of ASLV reverse transcriptases composed of multiple subunits
DE19720839B4 (de) 1997-05-17 2007-08-16 Dirk Dr. Schindelhauer Baukastenprinzip-Technologie zur Herstellung von langen, exakten DNA-Konstrukten in vitro, insbesondere für die Herstellung von Konstrukten zur Erzeugung von künstlichen Chromosomen (MAC, mammalian artificial chromosome) aus zwei stabil klonierten DNA-Komponenten und der ditelomerische Vektor-Prototyp PTAT
JP2001515234A (ja) 1997-07-25 2001-09-18 アフィメトリックス インコーポレイテッド 多型性データベースを提供するためのシステム
US5989872A (en) 1997-08-12 1999-11-23 Clontech Laboratories, Inc. Methods and compositions for transferring DNA sequence information among vectors
US7135608B1 (en) 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
US5888795A (en) 1997-09-09 1999-03-30 Becton, Dickinson And Company Thermostable uracil DNA glycosylase and methods of use
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
CN101125873A (zh) 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
EP1025217B1 (en) 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型ｌｏｘＰ配列とその応用
CA2306188C (en) * 1997-11-18 2008-08-05 Pioneer Hi-Bred International, Inc. A novel method for the integration of foreign dna into eukaryotic genomes
US5874259A (en) 1997-11-21 1999-02-23 Wisconsin Alumni Research Foundation Conditionally amplifiable BAC vector
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
ATE260989T1 (de) 1997-12-19 2004-03-15 Affymetrix Inc Erkenntnisse der genomforschung für die suche nach neuartigen wirkstoffen
US6150147A (en) 1998-02-06 2000-11-21 Affymetrix, Inc. Biological array fabrication methods with reduction of static charge
US6436707B1 (en) 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6080576A (en) 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
AU3928899A (en) 1998-04-28 1999-11-16 Novartis Ag Site-directed transformation of plants
AU5584999A (en) 1998-08-28 2000-03-21 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
AU1721600A (en) 1998-11-13 2000-06-05 Life Technologies, Inc. Compositions and methods for recombinational cloning of nucleic acid molecules
US6576463B1 (en) 1999-01-15 2003-06-10 The Regents Of The University Of California Hybrid vectors for gene therapy
US6120814A (en) 1999-02-22 2000-09-19 Nature's One, Inc. Organic nutritional formula
JP2002537800A (ja) 1999-03-02 2002-11-12 インビトロゲン・コーポレーション 毒性遺伝子に対して耐性のある細胞およびその使用
EP2119788A1 (en) 1999-03-02 2009-11-18 Life Technologies Corporation Compositions and methods for use in recombinational cloning of nucleic acids
CA2362737A1 (en) 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
WO2000060091A2 (en) 1999-04-06 2000-10-12 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
EP1194540A1 (en) 1999-07-14 2002-04-10 Clontech Laboratories Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
JP4808880B2 (ja) 1999-07-23 2011-11-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア バクテリオファージｐｈｉｃ３１組み換え系による真核細胞におけるｄｎａ組み換え
AU6529900A (en) 1999-08-09 2001-03-05 Monsanto Technology Llc Novel cloning methods and vectors
DE19941186A1 (de) 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
JP2003509060A (ja) 1999-09-17 2003-03-11 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 逆トランスフェクション方法
GB9923558D0 (en) 1999-10-05 1999-12-08 Oxford Biomedica Ltd Producer cell
NZ518503A (en) 1999-10-25 2004-02-27 Invitrogen Corp Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
WO2001032915A2 (en) 1999-11-04 2001-05-10 Genentech, Inc. Reversed-phase hplc assay for plasminogen activators
EP2210948A3 (en) 1999-12-10 2010-10-06 Life Technologies Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
CA2401632A1 (en) 2000-02-25 2001-08-30 Stratagene Method for ligating nucleic acids and molecular cloning
US6451602B1 (en) 2000-03-02 2002-09-17 Isis Pharmaceuticals, Inc. Antisense modulation of PARP expression
EP1272630A2 (en) 2000-03-16 2003-01-08 Genetica, Inc. Methods and compositions for rna interference
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20020068290A1 (en) 2000-05-31 2002-06-06 Timur Yarovinsky Topoisomerase activated oligonucleotide adaptors and uses therefor
US6551828B1 (en) 2000-06-28 2003-04-22 Protemation, Inc. Compositions and methods for generating expression vectors through site-specific recombination
GB0016747D0 (en) 2000-07-08 2000-08-30 Johnson Matthey Plc Ink
CA2417130A1 (en) 2000-07-25 2002-01-31 National Research Council Of Canada Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate acyltransferases
AU8835601A (en) 2000-08-21 2002-03-04 Invitrogen Corp Methods and reagents for molecular cloning
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002061034A2 (en) 2000-12-08 2002-08-08 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
ATE460472T1 (de) 2000-12-08 2010-03-15 Life Technologies Corp Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen
AU2002311756A1 (en) 2001-01-18 2002-10-28 Clontech Laboratories, Inc. Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same
NZ527679A (en) 2001-02-07 2005-07-29 Invitrogen Corp Nucleic acid Ter-(termination sequence) sites and Ter-binding proteins or RTPs in vitro and in vivo or combinations thereof
JP2004531259A (ja) 2001-04-19 2004-10-14 インヴィトロジェン コーポレーション 核酸分子の組換えクローニングのための組成物および方法
EP1451344A4 (en) 2001-05-21 2005-03-23 Invitrogen Corp COMPOSITIONS AND METHODS FOR USE IN ISOLATING NUCLEIC ACID MOLECULES
EP1427816A4 (en) 2001-09-18 2004-12-01 Clontech Lab Inc METHOD FOR THE PRODUCTION OF ADENOVIRUS VECTORS, BASED ON A SPECIFIC RECOMBINASE
US20030135888A1 (en) 2001-09-26 2003-07-17 Tong Zhu Genes that are modulated by posttranscriptional gene silencing
JP4210769B2 (ja) 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
US7294504B1 (en) 2001-12-27 2007-11-13 Allele Biotechnology & Pharmaceuticals, Inc. Methods and compositions for DNA mediated gene silencing
WO2003066829A2 (en) 2002-02-07 2003-08-14 Discovery Genomics, Inc. Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation and methods of use thereof
JP2005532829A (ja) 2002-07-18 2005-11-04 インヴィトロジェン コーポレーション 組換え部位を含むウイルスベクター
WO2004013290A2 (en) 2002-08-05 2004-02-12 Invitrogen Corporation Compositions and methods for molecular biology
WO2005028615A2 (en) 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
EP2216415B2 (en) 2003-08-01 2017-01-04 Life Technologies Corporation Methods for preparing short RNA molecules
WO2005014796A2 (en) 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
WO2005054427A2 (en) 2003-10-22 2005-06-16 Invitrogen Corporation Target sequences for synthetic molecules
US8972161B1 (en) 2005-11-17 2015-03-03 Invent.Ly, Llc Power management systems and devices
US12239299B2 (en) 2010-02-25 2025-03-04 Pave, Llc Full core biopsy device
WO2022165807A1 (zh) 2021-02-07 2022-08-11 京东方科技集团股份有限公司 显示基板、显示面板和显示装置
US12604999B2 (en) 2021-07-05 2026-04-21 Wei He Series of flower baskets

Also Published As

Publication number Publication date
WO2001042509A9 (en) 2002-05-30
CN1757724B (zh) 2014-06-11
JP2004500061A (ja) 2004-01-08
EP1250453A4 (en) 2004-04-07
CN1757724A (zh) 2006-04-12
US20020007051A1 (en) 2002-01-17
NZ519217A (en) 2004-03-26
EP1250453B1 (en) 2008-04-09
EP2210948A2 (en) 2010-07-28
WO2001042509A1 (en) 2001-06-14
AU2007221891A1 (en) 2007-11-01
AU785483B2 (en) 2007-09-20
DE60038572T2 (de) 2009-04-30
EP1978098A2 (en) 2008-10-08
DE60038572D1 (de) 2008-05-21
NZ530816A (en) 2005-10-28
CA2392753A1 (en) 2001-06-14
AU2085101A (en) 2001-06-18
EP1250453A1 (en) 2002-10-23
HK1062034A1 (zh) 2004-10-15
US7393632B2 (en) 2008-07-01
NZ539430A (en) 2006-09-29
EP2210948A3 (en) 2010-10-06
CN100587068C (zh) 2010-02-03
DK1250453T3 (da) 2008-08-11
ATE391789T1 (de) 2008-04-15
CN1468317A (zh) 2004-01-14
EP1978098A3 (en) 2009-02-18

Similar Documents

Publication Publication Date Title
JP4729222B2 (ja) 組換えクローニングにおける独自の選択性を有する複数の組換え部位の使用
JP2004500061A5 (https=)
US7351578B2 (en) Use of multiple recombination sites with unique specificity in recombinational cloning
US20140206577A1 (en) Compositions and methods for molecular biology
JP4731078B2 (ja) 転位および組換えを使用して核酸分子を操作および配列決定する方法
JP4394878B2 (ja) 複数の認識部位を用いた核酸分子合成のための方法および組成物
US20110306098A1 (en) Compositions and methods for use in isolation of nucleic acid molecules
CN110462034A (zh) 化脓链球菌cas9突变基因和由其编码的多肽
HK1062034B (en) Use of multiple recombination sites with unique specificity in recombinational cloning
Vasiliev et al. Isolation and crystallization of a chimeric Qβ replicase containing Thermus thermophilus EF-Ts
JP2004041083A (ja) 二本鎖ｄｎａ分子の効率的合成方法
AU2002316143A1 (en) Compositions and methods for use in isolation of nucleic acid molecules

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20070627

RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20070627

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20070710

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20070627

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20071210

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100901

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20101129

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20101206

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110228

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20110323

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20110418

R150 Certificate of patent or registration of utility model

Ref document number: 4729222

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140422

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term