JP2020506667A5 - - Google Patents
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- JP2020506667A5 JP2020506667A5 JP2019533098A JP2019533098A JP2020506667A5 JP 2020506667 A5 JP2020506667 A5 JP 2020506667A5 JP 2019533098 A JP2019533098 A JP 2019533098A JP 2019533098 A JP2019533098 A JP 2019533098A JP 2020506667 A5 JP2020506667 A5 JP 2020506667A5
- Authority
- JP
- Japan
- Prior art keywords
- promoter
- sequence
- polynucleotide
- acid sequence
- thermocas9
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EPPCT/EP2016/081077 | 2016-12-14 | ||
| PCT/EP2016/081077 WO2018108272A1 (en) | 2016-12-14 | 2016-12-14 | Thermostable cas9 nucleases |
| PCT/EP2017/070796 WO2018108338A1 (en) | 2016-12-14 | 2017-08-16 | Thermostable cas9 nucleases |
| EPPCT/EP2017/070796 | 2017-08-16 | ||
| PCT/EP2017/082870 WO2018109101A1 (en) | 2016-12-14 | 2017-12-14 | Thermostable cas9 nucleases |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020506667A JP2020506667A (ja) | 2020-03-05 |
| JP2020506667A5 true JP2020506667A5 (https=) | 2021-01-28 |
| JP7283698B2 JP7283698B2 (ja) | 2023-05-30 |
Family
ID=57755254
Family Applications (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019533096A Active JP7182545B2 (ja) | 2016-12-14 | 2016-12-14 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2019533103A Active JP7223377B2 (ja) | 2016-12-14 | 2017-08-16 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2019532936A Active JP7223376B2 (ja) | 2016-12-14 | 2017-08-16 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2019533098A Active JP7283698B2 (ja) | 2016-12-14 | 2017-12-14 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2022121297A Withdrawn JP2022166045A (ja) | 2016-12-14 | 2022-07-29 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2022128902A Withdrawn JP2022166170A (ja) | 2016-12-14 | 2022-08-12 | 熱安定性ｃａｓ９ヌクレアーゼ |
Family Applications Before (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019533096A Active JP7182545B2 (ja) | 2016-12-14 | 2016-12-14 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2019533103A Active JP7223377B2 (ja) | 2016-12-14 | 2017-08-16 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2019532936A Active JP7223376B2 (ja) | 2016-12-14 | 2017-08-16 | 熱安定性ｃａｓ９ヌクレアーゼ |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022121297A Withdrawn JP2022166045A (ja) | 2016-12-14 | 2022-07-29 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2022128902A Withdrawn JP2022166170A (ja) | 2016-12-14 | 2022-08-12 | 熱安定性ｃａｓ９ヌクレアーゼ |
Country Status (11)
| Country | Link |
|---|---|
| US (5) | US11242513B2 (https=) |
| EP (4) | EP3555275A1 (https=) |
| JP (6) | JP7182545B2 (https=) |
| KR (4) | KR102913446B1 (https=) |
| CN (4) | CN110312792B (https=) |
| AU (4) | AU2016432443B2 (https=) |
| BR (3) | BR112019012155A2 (https=) |
| CA (4) | CA3046824A1 (https=) |
| EA (4) | EA201991443A1 (https=) |
| PH (4) | PH12019501344A1 (https=) |
| WO (4) | WO2018108272A1 (https=) |
Families Citing this family (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9637739B2 (en) * | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| EP4464338A3 (en) | 2014-11-07 | 2025-02-12 | Editas Medicine, Inc. | Systems for improving crispr/cas-mediated genome-editing |
| CA2986310A1 (en) | 2015-05-11 | 2016-11-17 | Editas Medicine, Inc. | Optimized crispr/cas9 systems and methods for gene editing in stem cells |
| CN108026526B (zh) | 2015-06-09 | 2023-05-12 | 爱迪塔斯医药公司 | 用于改善移植的crispr/cas相关方法和组合物 |
| GB201510296D0 (en) * | 2015-06-12 | 2015-07-29 | Univ Wageningen | Thermostable CAS9 nucleases |
| EP3353296B1 (en) | 2015-09-24 | 2020-11-04 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| US11236313B2 (en) | 2016-04-13 | 2022-02-01 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| BR112019012155A2 (pt) | 2016-12-14 | 2019-11-12 | Wageningen Universiteit | uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína |
| EP3555297A1 (en) | 2016-12-19 | 2019-10-23 | Editas Medicine, Inc. | Assessing nuclease cleavage |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| JP7229923B2 (ja) | 2017-01-06 | 2023-02-28 | エディタス・メディシン、インコーポレイテッド | ヌクレアーゼ切断を評価する方法 |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11499151B2 (en) | 2017-04-28 | 2022-11-15 | Editas Medicine, Inc. | Methods and systems for analyzing guide RNA molecules |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| MX2019014640A (es) | 2017-06-09 | 2020-10-05 | Editas Medicine Inc | Nucleasas de repeticiones palíndromicas agrupadas y regularmente interespaciadas de proteina asociada 9 (cas9) genomanipuladas. |
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| GB201716590D0 (en) * | 2017-10-10 | 2017-11-22 | Univ Wageningen | Thermostable cas9 nucleases with reduced off-target activity |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| KR102465067B1 (ko) | 2018-02-15 | 2022-11-10 | 시그마-알드리치 컴퍼니., 엘엘씨 | 진핵 게놈 변형을 위한 조작된 cas9 시스템 |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| EP3823633A4 (en) | 2018-06-29 | 2023-05-03 | Editas Medicine, Inc. | SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| EP3874048A1 (en) | 2018-11-01 | 2021-09-08 | Keygene N.V. | Dual guide rna for crispr/cas genome editing in plants cells |
| CN113330122A (zh) * | 2018-11-16 | 2021-08-31 | 德皮克斯公司 | 使用位点特异性核酸酶优化核酸的体外分离 |
| RU2712497C1 (ru) * | 2018-11-26 | 2020-01-29 | Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий | Средство разрезания ДНК на основе Cas9 белка из биотехнологически значимой бактерии Clostridium cellulolyticum |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| US10947517B2 (en) | 2019-02-15 | 2021-03-16 | Sigma-Aldrich Co. Llc | CRISPR/Cas fusion proteins and systems |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CN116694603A (zh) * | 2019-05-14 | 2023-09-05 | 深圳华大生命科学研究院 | 新型的Cas蛋白、Crispr-Cas系统及其在基因编辑领域中的用途 |
| WO2021017200A1 (zh) * | 2019-07-30 | 2021-02-04 | 湖北大学 | 一种基于运动发酵单胞菌的CRISPR-Cas系统、基因组编辑体系及其应用 |
| CN110331158B (zh) * | 2019-07-30 | 2021-09-14 | 湖北大学 | 基于运动发酵单胞菌内源CRISPR-Cas系统的多基因位点同时编辑方法及其应用 |
| CN112410234B (zh) * | 2019-08-21 | 2022-08-23 | 江南大学 | 一种多靶点编辑重组曲霉菌株的可视化筛选方法 |
| US11879134B1 (en) * | 2019-09-05 | 2024-01-23 | The Regents Of The University Of Colorado, A Body Corporate | Recombineering machinery to increase homology directed genome editing in thermophilic microbes |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| CN110951739B (zh) * | 2019-12-25 | 2021-03-30 | 江南大学 | 一种由高温诱导表达的启动子及其应用 |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| CN111778230A (zh) * | 2020-07-17 | 2020-10-16 | 山东舜丰生物科技有限公司 | 一种适用于Cas12蛋白的缓冲系统及其应用 |
| RU2749307C1 (ru) * | 2020-10-30 | 2021-06-08 | Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт сельскохозяйственной биотехнологии" (ФГБНУ ВНИИСБ) | Новая компактная нуклеаза CAS9 II типа из Anoxybacillus flavithermus |
| CN116334037A (zh) * | 2020-11-11 | 2023-06-27 | 山东舜丰生物科技有限公司 | 新型Cas酶和系统以及应用 |
| CN114480347B (zh) * | 2022-02-21 | 2022-12-23 | 中国科学院地球化学研究所 | 一种纯化Cas12a蛋白的方法 |
| CN114934031B (zh) | 2022-05-25 | 2023-08-01 | 广州瑞风生物科技有限公司 | 新型Cas效应蛋白、基因编辑系统及用途 |
| JP7152094B1 (ja) | 2022-06-30 | 2022-10-12 | リージョナルフィッシュ株式会社 | ｔｒａｃｒＲＮＡユニット、及びゲノム編集方法 |
| CN117701530A (zh) * | 2022-12-08 | 2024-03-15 | 广州瑞风生物科技有限公司 | Cas蛋白截短体、构建其的方法及其应用 |
| CN116144631B (zh) * | 2023-01-17 | 2023-09-15 | 华中农业大学 | 耐热型核酸内切酶及其介导的基因编辑系统 |
| WO2025107617A1 (zh) * | 2023-11-24 | 2025-05-30 | 华智生物技术有限公司 | CRISPR/Cas效应蛋白及系统 |
| US20250250641A1 (en) * | 2024-02-06 | 2025-08-07 | Florida State University Research Foundation, Inc. | Methods and compositions related to epigenetic editing by a methlylation-dependent cas9 |
| CN118956928B (zh) * | 2024-07-08 | 2025-10-21 | 宁夏大学 | 一种动态调控链霉菌次生代谢过程中嗅味物质含量的方法 |
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| ES2176082B1 (es) | 2000-05-31 | 2004-01-01 | Consejo Superior Investigacion | Bacterias con la capacidad de unir metales pesados y su empleo en la detoxificacion de medios contaminados con metales pesados. |
| US7220571B2 (en) | 2000-09-28 | 2007-05-22 | Archer-Daniels-Midland Company | Escherichia coli strains which over-produce L-threonine and processes for the production of L-threonine by fermentation |
| CN1526013A (zh) * | 2001-02-23 | 2004-09-01 | 诺维信公司 | 脂解酶基因 |
| US7670807B2 (en) | 2004-03-10 | 2010-03-02 | East Tennessee State Univ. Research Foundation | RNA-dependent DNA polymerase from Geobacillus stearothermophilus |
| JP2006230303A (ja) | 2005-02-25 | 2006-09-07 | Art Engineering Kk | 好熱性リパーゼ産生菌およびその利用 |
| KR100872695B1 (ko) | 2006-11-27 | 2008-12-10 | 씨제이제일제당 (주) | Ｇｒａｓ 미생물로부터 발현된 식품안전형 호열성아라비노스 이성화효소 및 그를 이용한 타가토스의제조방법 |
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| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| EP4136963B1 (en) | 2013-03-15 | 2026-01-21 | Cibus US LLC | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
| KR102450868B1 (ko) | 2014-03-14 | 2022-10-06 | 시버스 유에스 엘엘씨 | 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물 |
| JP6637026B2 (ja) | 2014-07-23 | 2020-01-29 | ピュラック バイオケム ビー． ブイ． | 遺伝子改変された（ｒ）−乳酸産生好熱性細菌 |
| US10725041B2 (en) | 2014-11-04 | 2020-07-28 | Versiti Blood Research Institute Foundation, Inc. | Method to bioengineer designer platelets using gene editing and stem cell methodologies |
| EP4464338A3 (en) | 2014-11-07 | 2025-02-12 | Editas Medicine, Inc. | Systems for improving crispr/cas-mediated genome-editing |
| KR102424626B1 (ko) | 2014-12-17 | 2022-07-25 | 이 아이 듀폰 디 네모아 앤드 캄파니 | 원형 폴리뉴클레오티드 변형 주형과 함께 가이드 RNA/Cas 엔도뉴클레아제 시스템을 이용하여 대장균에서 효율적으로 유전자 편집을 하기 위한 조성물 및 방법 |
| WO2016179038A1 (en) * | 2015-05-01 | 2016-11-10 | Spark Therapeutics, Inc. | ADENO-ASSOCIATED VIRUS-MEDIATED CRISPR-Cas9 TREATMENT OF OCULAR DISEASE |
| AU2016263026A1 (en) * | 2015-05-15 | 2017-11-09 | Pioneer Hi-Bred International, Inc. | Guide RNA/Cas endonuclease systems |
| GB201510296D0 (en) * | 2015-06-12 | 2015-07-29 | Univ Wageningen | Thermostable CAS9 nucleases |
| BR112019012155A2 (pt) | 2016-12-14 | 2019-11-12 | Wageningen Universiteit | uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína |
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2016
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- 2016-12-14 AU AU2016432443A patent/AU2016432443B2/en active Active
- 2016-12-14 KR KR1020197020534A patent/KR102913446B1/ko active Active
- 2016-12-14 JP JP2019533096A patent/JP7182545B2/ja active Active
- 2016-12-14 US US16/469,674 patent/US11242513B2/en active Active
- 2016-12-14 WO PCT/EP2016/081077 patent/WO2018108272A1/en not_active Ceased
- 2016-12-14 CA CA3046824A patent/CA3046824A1/en active Pending
- 2016-12-14 EA EA201991443A patent/EA201991443A1/ru unknown
- 2016-12-14 CN CN201680092098.5A patent/CN110312792B/zh active Active
- 2016-12-14 EP EP16822931.8A patent/EP3555275A1/en active Pending
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2017
- 2017-08-16 EA EA201991440A patent/EA201991440A1/ru unknown
- 2017-08-16 US US16/469,683 patent/US11976306B2/en active Active
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- 2017-08-16 EA EA201991442A patent/EA201991442A1/ru unknown
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- 2017-12-14 BR BR112019012183-8A patent/BR112019012183A2/pt active IP Right Grant
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- 2017-12-14 EA EA201991441A patent/EA201991441A1/ru unknown
- 2017-12-14 KR KR1020197020537A patent/KR102662270B1/ko active Active
- 2017-12-14 EP EP17829169.6A patent/EP3555278B1/en active Active
- 2017-12-14 AU AU2017377792A patent/AU2017377792B2/en active Active
- 2017-12-14 CN CN201780086546.5A patent/CN110431229B/zh active Active
- 2017-12-14 CA CA3046842A patent/CA3046842A1/en active Pending
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2019
- 2019-06-13 PH PH12019501344A patent/PH12019501344A1/en unknown
- 2019-06-13 PH PH12019501346A patent/PH12019501346A1/en unknown
- 2019-06-13 PH PH12019501340A patent/PH12019501340A1/en unknown
- 2019-06-13 PH PH12019501335A patent/PH12019501335A1/en unknown
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2022
- 2022-01-14 US US17/575,952 patent/US11939605B2/en active Active
- 2022-07-29 JP JP2022121297A patent/JP2022166045A/ja not_active Withdrawn
- 2022-08-12 JP JP2022128902A patent/JP2022166170A/ja not_active Withdrawn
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