GB201716590D0 - Thermostable cas9 nucleases with reduced off-target activity - Google Patents

Thermostable cas9 nucleases with reduced off-target activity

Info

Publication number
GB201716590D0
GB201716590D0 GBGB1716590.3A GB201716590A GB201716590D0 GB 201716590 D0 GB201716590 D0 GB 201716590D0 GB 201716590 A GB201716590 A GB 201716590A GB 201716590 D0 GB201716590 D0 GB 201716590D0
Authority
GB
United Kingdom
Prior art keywords
reduced
target activity
cas9 nucleases
thermostable cas9
thermostable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB1716590.3A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wageningen Universiteit
Original Assignee
Wageningen Universiteit
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wageningen Universiteit filed Critical Wageningen Universiteit
Priority to GBGB1716590.3A priority Critical patent/GB201716590D0/en
Publication of GB201716590D0 publication Critical patent/GB201716590D0/en
Priority to PCT/EP2018/076480 priority patent/WO2019072596A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
GBGB1716590.3A 2017-10-10 2017-10-10 Thermostable cas9 nucleases with reduced off-target activity Ceased GB201716590D0 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GBGB1716590.3A GB201716590D0 (en) 2017-10-10 2017-10-10 Thermostable cas9 nucleases with reduced off-target activity
PCT/EP2018/076480 WO2019072596A1 (en) 2017-10-10 2018-09-28 Thermostable cas9 nucleases with reduced off-target activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB1716590.3A GB201716590D0 (en) 2017-10-10 2017-10-10 Thermostable cas9 nucleases with reduced off-target activity

Publications (1)

Publication Number Publication Date
GB201716590D0 true GB201716590D0 (en) 2017-11-22

Family

ID=60326828

Family Applications (1)

Application Number Title Priority Date Filing Date
GBGB1716590.3A Ceased GB201716590D0 (en) 2017-10-10 2017-10-10 Thermostable cas9 nucleases with reduced off-target activity

Country Status (2)

Country Link
GB (1) GB201716590D0 (en)
WO (1) WO2019072596A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018201086A1 (en) 2017-04-28 2018-11-01 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
CN110997908A (en) 2017-06-09 2020-04-10 爱迪塔斯医药公司 Engineered CAS9 nucleases
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
JP7448120B2 (en) 2019-11-14 2024-03-12 国立研究開発法人農業・食品産業技術総合研究機構 Method for introducing genome editing enzymes into plant cells using plasma

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
EA201991443A1 (en) * 2016-12-14 2020-01-14 Вагенинген Университейт THERMAL STABLE NUCLEASES Cas9
WO2018109101A1 (en) * 2016-12-14 2018-06-21 Wageningen Universiteit Thermostable cas9 nucleases

Also Published As

Publication number Publication date
WO2019072596A1 (en) 2019-04-18

Similar Documents

Publication Publication Date Title
IL293323A (en) Crispr enzyme mutations reducing off-target effects
IL290678B1 (en) Novel crispr enzymes and systems
GB201510296D0 (en) Thermostable CAS9 nucleases
SG10201913786SA (en) Modified rna agents with reduced off-target effect
EP3359644A4 (en) Novel rna-guided nucleases and uses thereof
IL271342A (en) Nucleic acid-guided nucleases
IL256078B (en) Halogen-substituted phenoxyphenylamidines and the use thereof as fungicides
IL286785A (en) Enzymes and applications thereof
EP3448534A4 (en) Activity and workout updates
SG10202010311SA (en) Novel Crispr Enzymes and Systems
EP3701013A4 (en) Targeted endonuclease activity of the rna-guided endonuclease casx in eukaryotes
HUE060120T2 (en) Herbicidally active 4-difluormethylbenzoylamide
EP3309856A4 (en) Battery and assembled battery
GB201716590D0 (en) Thermostable cas9 nucleases with reduced off-target activity
ZA202003848B (en) Barley with increased hydrolytic enzyme activity
GB201518190D0 (en) Porphyrins as electrocatalysts and electrodes modified with same
PT3453720T (en) New thermostable phytases with high catalytic efficacy
GB201620194D0 (en) Electrode and electrchemical cell comprising the same
PT3397769T (en) Microorganism with modified hydrogenase activity
IL250272A0 (en) Aglycosylated enzyme and uses thereof
GB201409709D0 (en) Improved deoxyribonuclease enzymes
GB201620551D0 (en) Enzymes and uses thereof
GB201701180D0 (en) Crispr rna
GB201700460D0 (en) Crispr rna
GB2537895B (en) Connector and assembly including the same

Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)