BR112019012173A2 - uso de pelo menos uma molécula de rna alvo e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína. - Google Patents

uso de pelo menos uma molécula de rna alvo e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína.

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Publication number
BR112019012173A2
BR112019012173A2 BR112019012173-0A BR112019012173A BR112019012173A2 BR 112019012173 A2 BR112019012173 A2 BR 112019012173A2 BR 112019012173 A BR112019012173 A BR 112019012173A BR 112019012173 A2 BR112019012173 A2 BR 112019012173A2
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BR
Brazil
Prior art keywords
cas protein
cleaving
labeling
modifying
binding
Prior art date
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BR112019012173-0A
Other languages
English (en)
Inventor
Fenna Bosma Elleke
Mougiakos Ioannis
Van Der Oost John
Mohanraju Prarthana
Van Kranenburg Richard
Original Assignee
Stichting Technische Wetenschappen
Univ Wageningen
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Application filed by Stichting Technische Wetenschappen, Univ Wageningen filed Critical Stichting Technische Wetenschappen
Publication of BR112019012173A2 publication Critical patent/BR112019012173A2/pt

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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Abstract

a presente invenção refere-se ao campo de engenharia genética e, mais particularmente, à edição de ácido nucleico e modificação do genoma. a presente invenção fornece uma proteína cas isolada ou fragmento de polipeptídeo da mesma tendo uma sequência de aminoácidos de seq id no: 1 ou uma sequência de pelo menos 77% de identidade com a mesma. a proteína cas ou polipeptídeo é capaz de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla a uma temperatura na faixa de 20°c e 100°c, inclusive. a invenção fornece adicionalmente moléculas de ácido nucleico isoladas codificando referidas nucleases cas9, vetores de expressão e células hospedeiras. a invenção também fornece sequências pam reconhecidas pela proteína cas ou polipeptídeo. as nucleases cas9 descritas aqui fornecem novas ferramentas para engenharia genética em geral, em particular em temperaturas elevadas.
BR112019012173-0A 2016-12-14 2017-08-16 uso de pelo menos uma molécula de rna alvo e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína. BR112019012173A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/EP2016/081077 WO2018108272A1 (en) 2016-12-14 2016-12-14 Thermostable cas9 nucleases
PCT/EP2017/070797 WO2018108339A1 (en) 2016-12-14 2017-08-16 Thermostable cas9 nucleases

Publications (1)

Publication Number Publication Date
BR112019012173A2 true BR112019012173A2 (pt) 2019-11-05

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Family Applications (4)

Application Number Title Priority Date Filing Date
BR112019012155-2A BR112019012155A2 (pt) 2016-12-14 2016-12-14 uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína
BR112019012165-0A BR112019012165A2 (pt) 2016-12-14 2017-08-16 uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada não humana, e, complexo de nucleoproteína
BR112019012173-0A BR112019012173A2 (pt) 2016-12-14 2017-08-16 uso de pelo menos uma molécula de rna alvo e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína.
BR112019012183-8A BR112019012183A2 (pt) 2016-12-14 2017-12-14 métodos de modificar o material genético de uma célula eucariótica e de um organismo, vetor de expressão de polinucleotídeo, e, célula procariótica.

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BR112019012155-2A BR112019012155A2 (pt) 2016-12-14 2016-12-14 uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína
BR112019012165-0A BR112019012165A2 (pt) 2016-12-14 2017-08-16 uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada não humana, e, complexo de nucleoproteína

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BR112019012183-8A BR112019012183A2 (pt) 2016-12-14 2017-12-14 métodos de modificar o material genético de uma célula eucariótica e de um organismo, vetor de expressão de polinucleotídeo, e, célula procariótica.

Country Status (11)

Country Link
US (5) US11242513B2 (pt)
EP (4) EP3555275A1 (pt)
JP (6) JP7182545B2 (pt)
KR (4) KR20190104342A (pt)
CN (4) CN110312792B (pt)
AU (4) AU2016432443B2 (pt)
BR (4) BR112019012155A2 (pt)
CA (4) CA3046824A1 (pt)
EA (4) EA201991443A1 (pt)
PH (4) PH12019501344A1 (pt)
WO (3) WO2018108272A1 (pt)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2853829C (en) 2011-07-22 2023-09-26 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US20150166982A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting pi3k point mutations
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
CA2986310A1 (en) 2015-05-11 2016-11-17 Editas Medicine, Inc. Optimized crispr/cas9 systems and methods for gene editing in stem cells
JP7396783B2 (ja) 2015-06-09 2023-12-12 エディタス・メディシン、インコーポレイテッド 移植を改善するためのｃｒｉｓｐｒ／ｃａｓ関連方法および組成物
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
CA2999500A1 (en) 2015-09-24 2017-03-30 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
EP4269577A3 (en) 2015-10-23 2024-01-17 President and Fellows of Harvard College Nucleobase editors and uses thereof
WO2017165826A1 (en) 2016-03-25 2017-09-28 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
EP4047092A1 (en) 2016-04-13 2022-08-24 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
SG11201900907YA (en) 2016-08-03 2019-02-27 Harvard College Adenosine nucleobase editors and uses thereof
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CN110214180A (zh) 2016-10-14 2019-09-06 哈佛大学的校长及成员们 核碱基编辑器的aav递送
EA201991443A1 (ru) 2016-12-14 2020-01-14 Вагенинген Университейт ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
EP3592777A1 (en) 2017-03-10 2020-01-15 President and Fellows of Harvard College Cytosine to guanine base editor
CN110914426A (zh) 2017-03-23 2020-03-24 哈佛大学的校长及成员们 包含核酸可编程dna结合蛋白的核碱基编辑器
WO2018201086A1 (en) 2017-04-28 2018-11-01 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
CN110997908A (zh) 2017-06-09 2020-04-10 爱迪塔斯医药公司 工程化的cas9核酸酶
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
GB201716590D0 (en) * 2017-10-10 2017-11-22 Univ Wageningen Thermostable cas9 nucleases with reduced off-target activity
JP2021500036A (ja) 2017-10-16 2021-01-07 ザ ブロード インスティテュート, インコーポレーテッドThe Broad Institute, Inc. アデノシン塩基編集因子の使用
KR102465067B1 (ko) * 2018-02-15 2022-11-10 시그마-알드리치 컴퍼니., 엘엘씨 진핵 게놈 변형을 위한 조작된 cas9 시스템
JP2022505440A (ja) 2018-11-01 2022-01-14 キージーン ナムローゼ フェンノートシャップ 植物細胞におけるＣＲＩＳＰＲ／Ｃａｓゲノム編集のためのデュアルガイドＲＮＡ
AU2020221274B2 (en) 2019-02-15 2024-02-08 Sigma-Aldrich Co. Llc. Crispr/Cas fusion proteins and systems
BR112021018607A2 (pt) 2019-03-19 2021-11-23 Massachusetts Inst Technology Métodos e composições para editar sequências de nucleotídeos
CN112301018B (zh) * 2019-05-14 2023-07-25 深圳华大生命科学研究院 新型的Cas蛋白、Crispr-Cas系统及其在基因编辑领域中的用途
US20220348939A1 (en) * 2019-07-30 2022-11-03 Hubei University CRISPR-Cas system for genome editing in Zymomonas mobilis, and applications thereof
CN110331158B (zh) * 2019-07-30 2021-09-14 湖北大学 基于运动发酵单胞菌内源CRISPR-Cas系统的多基因位点同时编辑方法及其应用
WO2021032180A1 (zh) * 2019-08-21 2021-02-25 江南大学 一种多靶点编辑重组曲霉菌株的可视化筛选方法
US11879134B1 (en) * 2019-09-05 2024-01-23 The Regents Of The University Of Colorado, A Body Corporate Recombineering machinery to increase homology directed genome editing in thermophilic microbes
CN116096873A (zh) 2020-05-08 2023-05-09 布罗德研究所股份有限公司 同时编辑靶标双链核苷酸序列的两条链的方法和组合物
CN111778230A (zh) * 2020-07-17 2020-10-16 山东舜丰生物科技有限公司 一种适用于Cas12蛋白的缓冲系统及其应用
RU2749307C1 (ru) * 2020-10-30 2021-06-08 Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт сельскохозяйственной биотехнологии" (ФГБНУ ВНИИСБ) Новая компактная нуклеаза CAS9 II типа из Anoxybacillus flavithermus
CN114480347B (zh) * 2022-02-21 2022-12-23 中国科学院地球化学研究所 一种纯化Cas12a蛋白的方法
CN114934031B (zh) * 2022-05-25 2023-08-01 广州瑞风生物科技有限公司 新型Cas效应蛋白、基因编辑系统及用途
JP7152094B1 (ja) 2022-06-30 2022-10-12 リージョナルフィッシュ株式会社 ｔｒａｃｒＲＮＡユニット、及びゲノム編集方法
CN117701530A (zh) * 2022-12-08 2024-03-15 广州瑞风生物科技有限公司 Cas蛋白截短体、构建其的方法及其应用
CN116144631B (zh) * 2023-01-17 2023-09-15 华中农业大学 耐热型核酸内切酶及其介导的基因编辑系统

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2176082B1 (es) 2000-05-31 2004-01-01 Consejo Superior Investigacion Bacterias con la capacidad de unir metales pesados y su empleo en la detoxificacion de medios contaminados con metales pesados.
US7220571B2 (en) 2000-09-28 2007-05-22 Archer-Daniels-Midland Company Escherichia coli strains which over-produce L-threonine and processes for the production of L-threonine by fermentation
WO2002066622A2 (en) * 2001-02-23 2002-08-29 Novozymes A/S Method of generating diversity into lipolytic enzymes and lipolytic enzyme genes
US7670807B2 (en) 2004-03-10 2010-03-02 East Tennessee State Univ. Research Foundation RNA-dependent DNA polymerase from Geobacillus stearothermophilus
JP2006230303A (ja) 2005-02-25 2006-09-07 Art Engineering Kk 好熱性リパーゼ産生菌およびその利用
KR100872695B1 (ko) 2006-11-27 2008-12-10 씨제이제일제당 (주) Ｇｒａｓ 미생물로부터 발현된 식품안전형 호열성아라비노스 이성화효소 및 그를 이용한 타가토스의제조방법
KR101491867B1 (ko) * 2007-01-31 2015-02-10 피페넥스 인크. 증가된 발현을 위한 박테리아 리더 서열
WO2011041402A1 (en) 2009-09-29 2011-04-07 Butamax(Tm) Advanced Biofuels Llc Improved flux to acetolactate-derived products in lactic acid bacteria
MX362866B (es) 2012-05-25 2019-02-20 Univ California Metodos y composiciones para la modificacion de adn objetivo dirigida por arn y para la modulacion de la transcripcion dirigida por arn.
RS63427B1 (sr) 2013-03-15 2022-08-31 Cibus Us Llc Postupci i kompozicije za povećanje efikasnosti modifikacije ciljnog gena korišćenjem genske reparacije posredovane oligonukleotidom
UA124375C2 (uk) 2014-03-14 2021-09-08 Кібус Юс Ллс Спосіб здійснення спрямованої генетичної зміни днк в клітині рослини з використанням нуклеази crispr і модифікованого gron
US10725041B2 (en) 2014-11-04 2020-07-28 Versiti Blood Research Institute Foundation, Inc. Method to bioengineer designer platelets using gene editing and stem cell methodologies
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
WO2016099887A1 (en) 2014-12-17 2016-06-23 E. I. Du Pont De Nemours And Company Compositions and methods for efficient gene editing in e. coli using guide rna/cas endonuclease systems in combination with circular polynucleotide modification templates
US20160346359A1 (en) * 2015-05-01 2016-12-01 Spark Therapeutics, Inc. Adeno-associated Virus-Mediated CRISPR-Cas9 Treatment of Ocular Disease
US20190136248A1 (en) * 2015-05-15 2019-05-09 Pioneer Hi-Bred International, Inc. Novel guide rna/cas endonuclease systems
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
EA201991443A1 (ru) 2016-12-14 2020-01-14 Вагенинген Университейт ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9

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