BR112019012165A2 - uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada não humana, e, complexo de nucleoproteína - Google Patents

uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada não humana, e, complexo de nucleoproteína

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Publication number
BR112019012165A2
BR112019012165A2 BR112019012165-0A BR112019012165A BR112019012165A2 BR 112019012165 A2 BR112019012165 A2 BR 112019012165A2 BR 112019012165 A BR112019012165 A BR 112019012165A BR 112019012165 A2 BR112019012165 A2 BR 112019012165A2
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Brazil
Prior art keywords
cas protein
cleaving
labeling
modifying
binding
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BR112019012165-0A
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English (en)
Inventor
Fenna Bosma Elleke
Mougiakos Ioannis
Van Der Oost John
Mohanraju Prarthana
Van Kranenburg Richard
Original Assignee
Stichting Technische Wetenschappen
Univ Wageningen
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Application filed by Stichting Technische Wetenschappen, Univ Wageningen filed Critical Stichting Technische Wetenschappen
Publication of BR112019012165A2 publication Critical patent/BR112019012165A2/pt

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Abstract

nucleases cas9 termoestáveis. a presente invenção refere-se ao campo de engenharia genética e, mais particularmente, à edição de ácido nucleico e modificação do genoma. a presente invenção fornece uma proteína cas isolada ou fragmento de polipeptídeo da mesma tendo uma sequência de aminoácidos de seq id no: 1 ou uma sequência de pelo menos 77% de identidade com a mesma. a proteína cas ou polipeptídeo é capaz de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla a uma temperatura na faixa de 20°c e 100°c, inclusive. a invenção fornece adicionalmente moléculas de ácido nucleico isoladas codificando referidas nucleases cas9, vetores de expressão e células hospedeiras. a invenção também fornece sequências pam reconhecidas pela proteína cas ou polipeptídeo. as nucleases cas9 descritas aqui fornecem novas ferramentas para engenharia genética em geral, em particular em temperaturas elevadas e são de valor particular na manipulação genética de organismos termofílicos; particularmente microrganismos.
BR112019012165-0A 2016-12-14 2017-08-16 uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada não humana, e, complexo de nucleoproteína BR112019012165A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/EP2016/081077 WO2018108272A1 (en) 2016-12-14 2016-12-14 Thermostable cas9 nucleases
PCT/EP2017/070796 WO2018108338A1 (en) 2016-12-14 2017-08-16 Thermostable cas9 nucleases

Publications (1)

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BR112019012165A2 true BR112019012165A2 (pt) 2019-11-12

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Application Number Title Priority Date Filing Date
BR112019012155-2A BR112019012155A2 (pt) 2016-12-14 2016-12-14 uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína
BR112019012173-0A BR112019012173A2 (pt) 2016-12-14 2017-08-16 uso de pelo menos uma molécula de rna alvo e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína.
BR112019012165-0A BR112019012165A2 (pt) 2016-12-14 2017-08-16 uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada não humana, e, complexo de nucleoproteína
BR112019012183-8A BR112019012183A2 (pt) 2016-12-14 2017-12-14 métodos de modificar o material genético de uma célula eucariótica e de um organismo, vetor de expressão de polinucleotídeo, e, célula procariótica.

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BR112019012155-2A BR112019012155A2 (pt) 2016-12-14 2016-12-14 uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína
BR112019012173-0A BR112019012173A2 (pt) 2016-12-14 2017-08-16 uso de pelo menos uma molécula de rna alvo e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína.

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BR112019012183-8A BR112019012183A2 (pt) 2016-12-14 2017-12-14 métodos de modificar o material genético de uma célula eucariótica e de um organismo, vetor de expressão de polinucleotídeo, e, célula procariótica.

Country Status (11)

Country Link
US (5) US11242513B2 (pt)
EP (4) EP3555275A1 (pt)
JP (6) JP7182545B2 (pt)
KR (4) KR20190104342A (pt)
CN (4) CN110312792B (pt)
AU (4) AU2016432443B2 (pt)
BR (4) BR112019012155A2 (pt)
CA (4) CA3046824A1 (pt)
EA (4) EA201991443A1 (pt)
PH (4) PH12019501346A1 (pt)
WO (3) WO2018108272A1 (pt)

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