EA201991442A1 - ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9 - Google Patents

ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9

Info

Publication number
EA201991442A1
EA201991442A1 EA201991442A EA201991442A EA201991442A1 EA 201991442 A1 EA201991442 A1 EA 201991442A1 EA 201991442 A EA201991442 A EA 201991442A EA 201991442 A EA201991442 A EA 201991442A EA 201991442 A1 EA201991442 A1 EA 201991442A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cas protein
relates
polypeptide
genetic engineering
cas9 nucleases
Prior art date
Application number
EA201991442A
Other languages
English (en)
Inventor
Джон Ван Дер Ост
Рихард Ван Краненбург
Эллеке Фенна Босма
Иоаннис Маугиакос
Прартхана Моханраджу
Original Assignee
Вагенинген Университейт
Стихтинг Вор Де Технише Ветенсхаппен
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Вагенинген Университейт, Стихтинг Вор Де Технише Ветенсхаппен filed Critical Вагенинген Университейт
Publication of EA201991442A1 publication Critical patent/EA201991442A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Abstract

Настоящее изобретение относится к области генной инженерии, и в частности к редактированию нуклеиновых кислот и модификации генома. Настоящее изобретение относится к выделенному белку Cas или его полипептидному фрагменту, имеющему аминокислотную последовательность SEQ ID NO: 1 или последовательность, идентичную ей по меньшей мере на 77%. Белок или полипептид Cas способен к связыванию, расщеплению, маркированию или модификации двухцепочечного полинуклеотида-мишени при температуре в диапазоне от 20 до 100°C включительно. Изобретение также относится к выделенным молекулам нуклеиновой кислоты, кодирующим указанные нуклеазы Cas9, экспрессионным векторам и клеткам-хозяевам. По изобретению также предложены последовательности PAM, узнаваемые белком или полипептидом Cas. Нуклеазы Cas9, раскрытые в настоящем документе, являются новыми инструментами для генетической инженерии в целом и в частности при повышенных температурах, и имеют особую ценность при генетических манипуляциях с термофильными организмами, в частности микроорганизмами.
EA201991442A 2016-12-14 2017-08-16 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9 EA201991442A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/EP2016/081077 WO2018108272A1 (en) 2016-12-14 2016-12-14 Thermostable cas9 nucleases
PCT/EP2017/070796 WO2018108338A1 (en) 2016-12-14 2017-08-16 Thermostable cas9 nucleases

Publications (1)

Publication Number Publication Date
EA201991442A1 true EA201991442A1 (ru) 2020-01-13

Family

ID=57755254

Family Applications (4)

Application Number Title Priority Date Filing Date
EA201991443A EA201991443A1 (ru) 2016-12-14 2016-12-14 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
EA201991442A EA201991442A1 (ru) 2016-12-14 2017-08-16 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
EA201991440A EA201991440A1 (ru) 2016-12-14 2017-08-16 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
EA201991441A EA201991441A1 (ru) 2016-12-14 2017-12-14 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EA201991443A EA201991443A1 (ru) 2016-12-14 2016-12-14 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9

Family Applications After (2)

Application Number Title Priority Date Filing Date
EA201991440A EA201991440A1 (ru) 2016-12-14 2017-08-16 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
EA201991441A EA201991441A1 (ru) 2016-12-14 2017-12-14 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9

Country Status (11)

Country Link
US (5) US11242513B2 (ru)
EP (4) EP3555275A1 (ru)
JP (6) JP7182545B2 (ru)
KR (3) KR20190104342A (ru)
CN (4) CN110312792B (ru)
AU (4) AU2016432443B2 (ru)
BR (4) BR112019012155A2 (ru)
CA (4) CA3046824A1 (ru)
EA (4) EA201991443A1 (ru)
PH (4) PH12019501335A1 (ru)
WO (3) WO2018108272A1 (ru)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
CA2963820A1 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
JP7030522B2 (ja) 2015-05-11 2022-03-07 エディタス・メディシン、インコーポレイテッド 幹細胞における遺伝子編集のための最適化ｃｒｉｓｐｒ／ｃａｓ９システムおよび方法
AU2016276702B2 (en) 2015-06-09 2022-07-28 Editas Medicine, Inc. CRISPR/CAS-related methods and compositions for improving transplantation
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
US11667911B2 (en) 2015-09-24 2023-06-06 Editas Medicine, Inc. Use of exonucleases to improve CRISPR/CAS-mediated genome editing
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
US11597924B2 (en) 2016-03-25 2023-03-07 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
EP4047092A1 (en) 2016-04-13 2022-08-24 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
GB2573062A (en) 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
WO2018108272A1 (en) 2016-12-14 2018-06-21 Wageningen Universiteit Thermostable cas9 nucleases
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20190130613A (ko) 2017-03-23 2019-11-22 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018201086A1 (en) 2017-04-28 2018-11-01 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US10428319B2 (en) 2017-06-09 2019-10-01 Editas Medicine, Inc. Engineered Cas9 nucleases
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
GB201716590D0 (en) * 2017-10-10 2017-11-22 Univ Wageningen Thermostable cas9 nucleases with reduced off-target activity
WO2019079347A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. USES OF BASIC EDITORS ADENOSINE
EP3752607A1 (en) * 2018-02-15 2020-12-23 Sigma Aldrich Co. LLC Engineered cas9 systems for eukaryotic genome modification
US20220010321A1 (en) 2018-11-01 2022-01-13 Keygene N.V. Dual guide rna for crispr/cas genome editing in plants cells
US10947517B2 (en) 2019-02-15 2021-03-16 Sigma-Aldrich Co. Llc CRISPR/Cas fusion proteins and systems
KR20210143230A (ko) 2019-03-19 2021-11-26 더 브로드 인스티튜트, 인코퍼레이티드 뉴클레오티드 서열을 편집하기 위한 방법 및 조성물
CN112301018B (zh) * 2019-05-14 2023-07-25 深圳华大生命科学研究院 新型的Cas蛋白、Crispr-Cas系统及其在基因编辑领域中的用途
CN110331158B (zh) * 2019-07-30 2021-09-14 湖北大学 基于运动发酵单胞菌内源CRISPR-Cas系统的多基因位点同时编辑方法及其应用
US20220348939A1 (en) * 2019-07-30 2022-11-03 Hubei University CRISPR-Cas system for genome editing in Zymomonas mobilis, and applications thereof
WO2021032180A1 (zh) * 2019-08-21 2021-02-25 江南大学 一种多靶点编辑重组曲霉菌株的可视化筛选方法
US11879134B1 (en) * 2019-09-05 2024-01-23 The Regents Of The University Of Colorado, A Body Corporate Recombineering machinery to increase homology directed genome editing in thermophilic microbes
CN116096873A (zh) 2020-05-08 2023-05-09 布罗德研究所股份有限公司 同时编辑靶标双链核苷酸序列的两条链的方法和组合物
CN111778230A (zh) * 2020-07-17 2020-10-16 山东舜丰生物科技有限公司 一种适用于Cas12蛋白的缓冲系统及其应用
RU2749307C1 (ru) * 2020-10-30 2021-06-08 Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт сельскохозяйственной биотехнологии" (ФГБНУ ВНИИСБ) Новая компактная нуклеаза CAS9 II типа из Anoxybacillus flavithermus
CN114480347B (zh) * 2022-02-21 2022-12-23 中国科学院地球化学研究所 一种纯化Cas12a蛋白的方法
CN114934031B (zh) * 2022-05-25 2023-08-01 广州瑞风生物科技有限公司 新型Cas效应蛋白、基因编辑系统及用途
JP7152094B1 (ja) 2022-06-30 2022-10-12 リージョナルフィッシュ株式会社 ｔｒａｃｒＲＮＡユニット、及びゲノム編集方法
CN116144631B (zh) * 2023-01-17 2023-09-15 华中农业大学 耐热型核酸内切酶及其介导的基因编辑系统

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2176082B1 (es) 2000-05-31 2004-01-01 Consejo Superior Investigacion Bacterias con la capacidad de unir metales pesados y su empleo en la detoxificacion de medios contaminados con metales pesados.
US7220571B2 (en) 2000-09-28 2007-05-22 Archer-Daniels-Midland Company Escherichia coli strains which over-produce L-threonine and processes for the production of L-threonine by fermentation
EP1404827A2 (en) * 2001-02-23 2004-04-07 Novozymes A/S Lipolytic enzyme genes
US7670807B2 (en) 2004-03-10 2010-03-02 East Tennessee State Univ. Research Foundation RNA-dependent DNA polymerase from Geobacillus stearothermophilus
JP2006230303A (ja) 2005-02-25 2006-09-07 Art Engineering Kk 好熱性リパーゼ産生菌およびその利用
KR100872695B1 (ko) 2006-11-27 2008-12-10 씨제이제일제당 (주) Ｇｒａｓ 미생물로부터 발현된 식품안전형 호열성아라비노스 이성화효소 및 그를 이용한 타가토스의제조방법
CN102517280A (zh) * 2007-01-31 2012-06-27 菲尼克斯股份有限公司 用于提高表达的细菌前导序列
CA2776151A1 (en) * 2009-09-29 2011-04-07 Butamax(Tm) Advanced Biofuels Llc Improved flux to acetolactate-derived products in lactic acid bacteria
JP6343605B2 (ja) * 2012-05-25 2018-06-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｒｎａ依存性標的ｄｎａ修飾およびｒｎａ依存性転写調節のための方法および組成物
IL307456A (en) 2013-03-15 2023-12-01 Cibus Us Llc Methods and compositions for increasing the efficiency of targeted gene modification using oligonucleotide-mediated gene repair
KR102450868B1 (ko) 2014-03-14 2022-10-06 시버스 유에스 엘엘씨 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물
US10725041B2 (en) 2014-11-04 2020-07-28 Versiti Blood Research Institute Foundation, Inc. Method to bioengineer designer platelets using gene editing and stem cell methodologies
CA2963820A1 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
EP3234117B1 (en) * 2014-12-17 2021-03-03 DuPont US Holding, LLC Compositions and methods for efficient gene editing in e. coli using guide rna/cas endonuclease systems in combination with circular polynucleotide modification templates
WO2016179038A1 (en) * 2015-05-01 2016-11-10 Spark Therapeutics, Inc. ADENO-ASSOCIATED VIRUS-MEDIATED CRISPR-Cas9 TREATMENT OF OCULAR DISEASE
AU2016265560B2 (en) 2015-05-15 2022-07-28 Pioneer Hi-Bred International, Inc. Rapid characterization of Cas endonuclease systems, PAM sequences and guide RNA elements
GB201510296D0 (en) 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
WO2018108272A1 (en) 2016-12-14 2018-06-21 Wageningen Universiteit Thermostable cas9 nucleases

Also Published As

Publication number Publication date
EA201991443A1 (ru) 2020-01-14
CN110431229B (zh) 2024-03-12
EA201991441A1 (ru) 2020-01-09
PH12019501340A1 (en) 2019-09-30
CN110352241B (zh) 2024-04-09
AU2017377135A1 (en) 2019-07-11
CA3046842A1 (en) 2018-06-21
BR112019012165A2 (pt) 2019-11-12
JP2022166045A (ja) 2022-11-01
CN110431229A (zh) 2019-11-08
JP2020505000A (ja) 2020-02-20
BR112019012173A2 (pt) 2019-11-05
CN110382693A (zh) 2019-10-25
EP3555278A1 (en) 2019-10-23
AU2016432443B2 (en) 2024-04-18
US11939605B2 (en) 2024-03-26
US11326162B2 (en) 2022-05-10
WO2018108338A1 (en) 2018-06-21
JP2022166170A (ja) 2022-11-01
BR112019012183A2 (pt) 2019-11-05
KR102523544B1 (ko) 2023-04-19
WO2018108272A1 (en) 2018-06-21
KR20190104343A (ko) 2019-09-09
KR102523543B1 (ko) 2023-04-19
US20210340532A1 (en) 2021-11-04
CN110312792B (zh) 2024-04-09
AU2017377135B2 (en) 2024-04-18
JP2020510410A (ja) 2020-04-09
WO2018108339A1 (en) 2018-06-21
AU2017377136A1 (en) 2019-07-11
BR112019012155A2 (pt) 2019-11-12
JP2020506667A (ja) 2020-03-05
AU2017377792A1 (en) 2019-07-11
KR20190104342A (ko) 2019-09-09
EP3555275A1 (en) 2019-10-23
CN110352241A (zh) 2019-10-18
AU2017377136B2 (en) 2024-04-18
PH12019501335A1 (en) 2019-09-16
PH12019501344A1 (en) 2019-10-28
CN110382693B (zh) 2024-04-09
JP2020504603A (ja) 2020-02-13
US20190367893A1 (en) 2019-12-05
KR20190104344A (ko) 2019-09-09
US11242513B2 (en) 2022-02-08
AU2016432443A1 (en) 2019-07-11
JP7283698B2 (ja) 2023-05-30
US20190322993A1 (en) 2019-10-24
JP7182545B2 (ja) 2022-12-02
EA201991440A1 (ru) 2020-01-13
US20220213455A1 (en) 2022-07-07
WO2018108339A9 (en) 2018-09-20
PH12019501346A1 (en) 2019-11-04
EP3555276A1 (en) 2019-10-23
AU2017377792B2 (en) 2024-04-18
EP3555277A1 (en) 2019-10-23
CA3046826A1 (en) 2018-06-21
KR20190104345A (ko) 2019-09-09
US20190360002A1 (en) 2019-11-28
JP7223377B2 (ja) 2023-02-16
CA3046828A1 (en) 2018-06-21
JP7223376B2 (ja) 2023-02-16
CA3046824A1 (en) 2018-06-21
CN110312792A (zh) 2019-10-08

Similar Documents

Publication Publication Date Title
EA201991442A1 (ru) ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
PH12017502281A1 (en) Thermostable cas9 nucleases
CA3166153A1 (en) Base editors, compositions, and methods for modifying the mitochondrial genome
Ma et al. Rapid decoding of sequence-specific nuclease-induced heterozygous and biallelic mutations by direct sequencing of PCR products
PH12021550904A1 (en) Novel crispr/cas12f enzyme and system
EA201892810A1 (ru) Гибридные последовательности нуклеиновых кислот для геномной инженерии
RU2022103603A (ru) Направляемые нуклеиновыми кислотами нуклеазы
RU2015112583A (ru) Функциональные локусы fad2 и соответствующие специфичные для сайта-мишени связывающиеся белки, способные индуцировать направленные разрывы
Pfeifer et al. Silencing of cryptic prophages in Corynebacterium glutamicum
MX2022005328A (es) Composiciones y metodos para el reemplazo de alelos con adn codificado por arn.
EA202190454A1 (ru) Новый crispr-ассоциированный белок и его применение
AU2021311773A8 (en) Novel, non-naturally occurring CRISPR-CAS nucleases for genome editing
CN102757960A (zh) 一种扩增未知序列的半随机pcr技术及其引物和试剂盒
SG11201809242VA (en) Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor
CA3228222A1 (en) Class ii, type v crispr systems
Chen et al. Aptazyme-mediated direct modulation of post-transcriptional sgRNA level for conditional genome editing and gene expression
MX2019009045A (es) Nuevas luciferasas y metodos para su uso.
WO2019204643A3 (en) Animal pathogen-derived polypeptides and uses thereof for genetic engineering
WO2018014799A1 (zh) 重组酶聚合酶扩增试剂盒、扩增方法及扩增试剂
Li et al. Clipping of predetermined fragments from the human genome by S1 nuclease–PNA combinations
CN107312788A (zh) 一种tale重复序列载体的构建方法
Ng Avoiding CRISPR: Plasmid design for genetic engineering
Chan Transcriptome Analysis of Bacterial Response to Heat Shock Using Next‐Generation Sequencing
KR20240055073A (ko) 클래스 ii, v형 crispr 시스템
Selkova et al. Characterization of Streptococcus uberis Cas9 (SuCas9)-a Type II-A Ortholog Functional in Human Cells