BR112022008415A2 - Composições e métodos para substituição do dna codificado pelo rna dos alelos - Google Patents

Composições e métodos para substituição do dna codificado pelo rna dos alelos

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Publication number
BR112022008415A2
BR112022008415A2 BR112022008415A BR112022008415A BR112022008415A2 BR 112022008415 A2 BR112022008415 A2 BR 112022008415A2 BR 112022008415 A BR112022008415 A BR 112022008415A BR 112022008415 A BR112022008415 A BR 112022008415A BR 112022008415 A2 BR112022008415 A2 BR 112022008415A2
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BR
Brazil
Prior art keywords
type
extended
crispr
nucleic acid
rna
Prior art date
Application number
BR112022008415A
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English (en)
Inventor
Matthew Watts Joseph
Hummel Aaron
Kim Yongjoo
Joshua Lawit Shai
Schwark David
Original Assignee
Pairwise Plants Services Inc
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Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of BR112022008415A2 publication Critical patent/BR112022008415A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Abstract

COMPOSIÇÕES E MÉTODOS PARA SUBSTITUIÇÃO DO DNA CODIFICADO PELO RNA DOS ALELOS. A presente invenção refere-se a construtos de ácido nucleico recombinantes compreendendo proteínas efetoras CRISPR-Cas do tipo V, transcriptases reversas e ácidos nucleicos guia estendidos e métodos de uso dos mesmos para modificar ácidos nucleicos em plantas. Em um primeiro aspecto, um método de modificação do ácido nucleico alvo é provido, o método compreendo: colocar um ácido nucleico alvo em contato com (a) uma proteína efetora CRISPR-Cas do Tipo V ou uma proteína efetora CRISPR-Cas do Tipo II; (b) uma transcriptase reversa, e (c) um ácido nucleico guia estendido (por exemplo, RNA CRISPR estendido do Tipo II ou do Tipo V, DNA CRISPR estendido do Tipo II ou do Tipo V, crRNA estendido do Tipo II ou do Tipo V, crDNA) estendido do Tipo II ou Tipo V, desse modo modificando o ácido nucleico alvo.
BR112022008415A 2019-11-05 2020-11-05 Composições e métodos para substituição do dna codificado pelo rna dos alelos BR112022008415A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962930836P 2019-11-05 2019-11-05
PCT/US2020/059045 WO2021092130A1 (en) 2019-11-05 2020-11-05 Compositions and methods for rna-encoded dna-replacement of alleles

Publications (1)

Publication Number Publication Date
BR112022008415A2 true BR112022008415A2 (pt) 2022-09-06

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Country Status (12)

Country Link
US (1) US11926834B2 (pt)
EP (1) EP4054621A4 (pt)
JP (1) JP2023505600A (pt)
KR (1) KR20220110748A (pt)
CN (1) CN114901302A (pt)
AU (1) AU2020380319A1 (pt)
BR (1) BR112022008415A2 (pt)
CA (1) CA3160186A1 (pt)
CL (1) CL2022001145A1 (pt)
IL (1) IL292434A (pt)
MX (1) MX2022005328A (pt)
WO (1) WO2021092130A1 (pt)

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US20210130835A1 (en) 2021-05-06
AU2020380319A1 (en) 2022-05-12
CL2022001145A1 (es) 2023-02-03
EP4054621A1 (en) 2022-09-14
EP4054621A4 (en) 2023-12-27
IL292434A (en) 2022-06-01
CN114901302A (zh) 2022-08-12
WO2021092130A1 (en) 2021-05-14
CA3160186A1 (en) 2021-05-14
MX2022005328A (es) 2022-07-21
US11926834B2 (en) 2024-03-12
JP2023505600A (ja) 2023-02-09
KR20220110748A (ko) 2022-08-09

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