BR112022026311A2 - Nucleases crispr-cas de ocorrência não natural para edição de genoma - Google Patents

Nucleases crispr-cas de ocorrência não natural para edição de genoma

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Publication number
BR112022026311A2
BR112022026311A2 BR112022026311A BR112022026311A BR112022026311A2 BR 112022026311 A2 BR112022026311 A2 BR 112022026311A2 BR 112022026311 A BR112022026311 A BR 112022026311A BR 112022026311 A BR112022026311 A BR 112022026311A BR 112022026311 A2 BR112022026311 A2 BR 112022026311A2
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BR
Brazil
Prior art keywords
nucleic acid
acid molecule
naturally occurring
genome editing
occurring crispr
Prior art date
Application number
BR112022026311A
Other languages
English (en)
Inventor
Scholz Paul
Zurek Christian
Krohn Michael
Original Assignee
BRAIN Biotech AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by BRAIN Biotech AG filed Critical BRAIN Biotech AG
Publication of BR112022026311A2 publication Critical patent/BR112022026311A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

NUCLEASES CRISPR-CAS DE OCORRÊNCIA NÃO NATURAL PARA EDIÇÃO DE GENOMA. A presente invenção refere-se a uma molécula de ácido nucleico que codifica uma DNA endonuclease guiada por RNA, que é (a) uma molécula de ácido nucleico que codifica a DNA endonuclease guiada por RNA compreendendo ou consistindo na sequência de aminoácidos de SEQ ID Nº: 29, 1 ou 3; (b) uma molécula de ácido nucleico compreendendo ou consistindo na sequência nucleotídica de SEQ ID Nº: 30, 2 ou 4; (c) uma molécula de ácido nucleico que codifica uma DNA endonuclease guiada por RNA cuja sequência de aminoácidos é pelo menos 90%, preferencialmente pelo menos 92% e mais preferencialmente pelo menos 95% idêntica à sequência de aminoácidos de (a); (d) uma molécula de ácido nucleico compreendendo ou consistindo em uma sequência nucleotídica que é pelo menos 90%, preferencialmente pelo menos 92% e mais prefe-rencialmente pelo menos 95% idêntica à sequência nucleotídica de (b); (e) uma molécula de ácido nucleico que é degenerada em relação à molécula de ácido nucleico de (d); ou (f) uma molécula de ácido nucleico correspondente à molécula de ácido nucleico de qualquer um de (a) a (d) em que T é substituído por U.
BR112022026311A 2020-07-21 2021-07-20 Nucleases crispr-cas de ocorrência não natural para edição de genoma BR112022026311A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20000262 2020-07-21
EP20000329.1A EP3943600A1 (en) 2020-07-21 2020-09-10 Novel, non-naturally occurring crispr-cas nucleases for genome editing
PCT/EP2021/000081 WO2022017633A2 (en) 2020-07-21 2021-07-20 Novel, non-naturally occurring crispr-cas nucleases for genome editing

Publications (1)

Publication Number Publication Date
BR112022026311A2 true BR112022026311A2 (pt) 2023-01-31

Family

ID=71783795

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022026311A BR112022026311A2 (pt) 2020-07-21 2021-07-20 Nucleases crispr-cas de ocorrência não natural para edição de genoma

Country Status (14)

Country Link
US (1) US20230287372A1 (pt)
EP (3) EP3943600A1 (pt)
JP (1) JP2023535064A (pt)
KR (1) KR20230041687A (pt)
CN (1) CN116134133A (pt)
AU (1) AU2021311773A1 (pt)
BR (1) BR112022026311A2 (pt)
CA (1) CA3189525A1 (pt)
DK (1) DK4025691T3 (pt)
ES (1) ES2963187T3 (pt)
FI (1) FI4025691T3 (pt)
IL (1) IL299990A (pt)
PL (1) PL4025691T3 (pt)
WO (1) WO2022017633A2 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117255855A (zh) * 2021-03-02 2023-12-19 Brain生物技术有限公司 来自宏基因组的新颖的CRISPR-Cas核酸酶
WO2023139096A1 (en) * 2022-01-19 2023-07-27 BRAIN Biotech AG Depletion of cells by crispr nucleases
EP4215611A1 (en) 2022-01-19 2023-07-26 BRAIN Biotech AG Modification of the genome of a filamentous fungus
EP4215612A1 (en) 2022-01-20 2023-07-26 Sartorius Stedim Cellca GmbH Genome editing with crispr/cas nucleases comprising collateral activity
EP4361265A1 (en) * 2022-10-27 2024-05-01 BRAIN Biotech AG Optimization of editing efficacy of crispr nucleases with collateral activity

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6015891A (en) 1988-09-09 2000-01-18 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene having a modified frequency of codon usage
WO1990011361A1 (en) 1989-03-17 1990-10-04 E.I. Du Pont De Nemours And Company External regulation of gene expression
US20030167526A1 (en) 2002-01-14 2003-09-04 Pioneer Hi-Bred International Inc. Compositions and methods for identifying transformed cells
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
US9896696B2 (en) 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
US20180362590A1 (en) * 2017-04-14 2018-12-20 Synthetic Genomics, Inc. Polypeptides with type v crispr activity and uses thereof
IL310452A (en) 2017-08-09 2024-03-01 Ricetec Inc Preparations and methods for genome modification

Also Published As

Publication number Publication date
WO2022017633A8 (en) 2022-04-07
US20230287372A1 (en) 2023-09-14
PL4025691T3 (pl) 2024-04-08
EP4025691B1 (en) 2023-09-13
WO2022017633A3 (en) 2022-03-10
FI4025691T3 (fi) 2023-11-06
CN116134133A (zh) 2023-05-16
AU2021311773A1 (en) 2023-02-23
KR20230041687A (ko) 2023-03-24
AU2021311773A8 (en) 2023-03-09
EP4279597A3 (en) 2024-04-24
IL299990A (en) 2023-03-01
EP3943600A1 (en) 2022-01-26
JP2023535064A (ja) 2023-08-15
EP4025691A2 (en) 2022-07-13
WO2022017633A2 (en) 2022-01-27
DK4025691T3 (da) 2023-10-30
ES2963187T3 (es) 2024-03-25
EP4279597A2 (en) 2023-11-22
CA3189525A1 (en) 2022-01-27

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