CL2022001145A1 - Composiciones y métodos para el reemplazo de alelos de adn codificado por arn - Google Patents

Composiciones y métodos para el reemplazo de alelos de adn codificado por arn

Info

Publication number
CL2022001145A1
CL2022001145A1 CL2022001145A CL2022001145A CL2022001145A1 CL 2022001145 A1 CL2022001145 A1 CL 2022001145A1 CL 2022001145 A CL2022001145 A CL 2022001145A CL 2022001145 A CL2022001145 A CL 2022001145A CL 2022001145 A1 CL2022001145 A1 CL 2022001145A1
Authority
CL
Chile
Prior art keywords
type
extended
crispr
nucleic acid
methods
Prior art date
Application number
CL2022001145A
Other languages
English (en)
Inventor
Joseph Matthew Watts
Aaron Hummel
Yongjoo Kim
Shai Joshua Lawit
David Schwark
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of CL2022001145A1 publication Critical patent/CL2022001145A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1276RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/85Fusion polypeptide containing an RNA binding domain
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07049RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Esta invención se refiere a construcciones nucleicas recombinantes que comprenden proteínas efectoras CRISPR–Cas Tipo V, transcriptasas inversas y ácidos nucleicos guía extendidos, y a métodos de uso de los mismos para modificar ácidos nucleicos en plantas. En un primer aspecto, se proporciona un método para modificar un ácido nucleico diana, donde el método comprende: poner en contacto el ácido nucleico diana con (a) una proteína efectora CRISPR–Cas Tipo V o una proteína efectora CRISPR–Cas Tipo II; (b) una transcriptasa inversa, y (c) un ácido nucleico guía extendido (p. ej., ARN CRISPR Tipo II o Tipo V extendido, ADN CRISPR Tipo II o Tipo V extendido, ARNcr Tipo II o Tipo V extendido, ADNcr Tipo II o Tipo V extendido), de modo de modificar así el ácido nucleico diana.
CL2022001145A 2019-11-05 2022-05-03 Composiciones y métodos para el reemplazo de alelos de adn codificado por arn CL2022001145A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201962930836P 2019-11-05 2019-11-05

Publications (1)

Publication Number Publication Date
CL2022001145A1 true CL2022001145A1 (es) 2023-02-03

Family

ID=75687119

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2022001145A CL2022001145A1 (es) 2019-11-05 2022-05-03 Composiciones y métodos para el reemplazo de alelos de adn codificado por arn

Country Status (12)

Country Link
US (3) US11926834B2 (es)
EP (1) EP4054621A4 (es)
JP (1) JP2023505600A (es)
KR (1) KR20220110748A (es)
CN (1) CN114901302A (es)
AU (1) AU2020380319A1 (es)
BR (1) BR112022008415A2 (es)
CA (1) CA3160186A1 (es)
CL (1) CL2022001145A1 (es)
IL (1) IL292434A (es)
MX (1) MX2022005328A (es)
WO (1) WO2021092130A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112022008415A2 (pt) 2019-11-05 2022-09-06 Pairwise Plants Services Inc Composições e métodos para substituição do dna codificado pelo rna dos alelos
KR20230106633A (ko) * 2020-11-06 2023-07-13 페어와이즈 플랜츠 서비시즈, 인크. 대립유전자의 rna-코딩된 dna-대체를 위한 조성물 및 방법
US20240102007A1 (en) * 2021-06-01 2024-03-28 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof
US20220403475A1 (en) 2021-06-14 2022-12-22 Pairwise Plants Services, Inc. Reporter constructs, compositions comprising the same, and methods of use thereof
AU2022343268A1 (en) * 2021-09-08 2024-03-28 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
WO2023049728A1 (en) 2021-09-21 2023-03-30 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
WO2023102329A2 (en) * 2021-11-30 2023-06-08 Mammoth Biosciences, Inc. Effector proteins and uses thereof
CN114317492A (zh) * 2021-12-06 2022-04-12 北京大学 一种改造的人工核酸酶系统及其应用
WO2024020346A2 (en) * 2022-07-18 2024-01-25 Renagade Therapeutics Management Inc. Gene editing components, systems, and methods of use
WO2024064915A2 (en) * 2022-09-23 2024-03-28 Flagship Pioneering Innovations Vi, Llc Methods for enriching nucleic acid target sequences

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ221259A (en) 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
US6040504A (en) 1987-11-18 2000-03-21 Novartis Finance Corporation Cotton promoter
EP0342926B1 (en) 1988-05-17 1994-09-28 Mycogen Plant Science, Inc. Plant ubiquitin promoter system
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
EP0452269B1 (en) 1990-04-12 2002-10-09 Syngenta Participations AG Tissue-preferential promoters
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
EP0600993B1 (en) 1991-08-27 1999-11-10 Novartis AG Proteins with insecticidal properties against homopteran insects and their use in plant protection
UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
KR20010041129A (ko) 1998-02-20 2001-05-15 돈 리사 로얄 화분 특이적 프로모터
US7166770B2 (en) 2000-03-27 2007-01-23 Syngenta Participations Ag Cestrum yellow leaf curling virus promoters
AR044724A1 (es) 2000-03-27 2005-10-05 Syngenta Participations Ag Promotores del virus de la rizadura amarilla del cestrum
ATE495264T1 (de) 2003-10-06 2011-01-15 Syngenta Participations Ag In pflanzenplastiden funktionsfähiger promotor
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
ES2624276T3 (es) 2009-06-11 2017-07-13 Syngenta Participations Ag Un método para la expresión transitoria de ácidos nucleicos en plantas
RU2720973C2 (ru) 2012-02-01 2020-05-15 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Синтетические транзитные пептиды хлоропластов
DE202013012597U1 (de) * 2012-10-23 2017-11-21 Toolgen, Inc. Zusammensetzung zum Spalten einer Ziel-DNA, umfassend eine für die Ziel-DNA spezifische guide-RNA und eine Cas-Protein-codierende Nukleinsäure oder ein Cas-Protein, sowie deren Verwendung
WO2014186686A2 (en) * 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
EP3611268A1 (en) 2013-08-22 2020-02-19 E. I. du Pont de Nemours and Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
EP3126498A4 (en) 2014-03-20 2017-08-23 Université Laval Crispr-based methods and products for increasing frataxin levels and uses thereof
EP3169702A4 (en) 2014-07-14 2018-04-18 The Regents of The University of California A protein tagging system for in vivo single molecule imaging and control of gene transcription
DK3177644T3 (da) 2014-08-05 2021-01-11 MabQuest SA Immunologiske reagenser, som binder til PD-1
EP3215617B1 (en) * 2014-11-07 2024-05-08 Editas Medicine, Inc. Systems for improving crispr/cas-mediated genome-editing
CN107787367B (zh) * 2015-04-06 2021-10-26 里兰斯坦福初级大学理事会 用于crispr/cas介导的基因调控的化学修饰的引导rna
US20160362667A1 (en) * 2015-06-10 2016-12-15 Caribou Biosciences, Inc. CRISPR-Cas Compositions and Methods
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
JP2018531024A (ja) 2015-10-20 2018-10-25 パイオニア ハイ−ブレッド インターナショナル, イン マーカーフリーゲノム改変のための方法および組成物
CA3004757C (en) 2015-12-04 2020-07-21 Caribou Biosciences, Inc. Engineered nucleic-acid targeting nucleic acids
US10538750B2 (en) 2016-02-29 2020-01-21 Agilent Technologies, Inc. Methods and compositions for blocking off-target nucleic acids from cleavage by CRISPR proteins
WO2017189308A1 (en) 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
EP3463484A4 (en) 2016-05-27 2019-10-30 The Regents of the University of California METHOD AND COMPOSITIONS FOR TARGETING RNA POLYMERASES AND NON-CODING RNA BIOGENESIS AT SPECIFIC LOCI
KR20190012229A (ko) 2016-06-01 2019-02-08 케이더블유에스 사아트 에스이 유전자 조작을 위한 하이브리드 핵산 서열
CN109790527A (zh) 2016-07-26 2019-05-21 通用医疗公司 普氏菌属和弗朗西斯氏菌属的CRISPR1(Cpf1)的变体
EP3510151A4 (en) 2016-09-09 2020-04-15 The Board of Trustees of the Leland Stanford Junior University HIGH-THROUGHPUT PRECISION GENE EDITING
AU2018209969B2 (en) 2017-01-20 2024-02-29 The Regents Of The University Of California Targeted gene activation in plants
CA3059956A1 (en) 2017-04-21 2018-10-25 The General Hospital Corporation Variants of cpf1 (cas12a) with altered pam specificity
CN108795972B (zh) 2017-05-05 2023-07-14 苏州齐禾生科生物科技有限公司 不使用转基因标记序列分离细胞的方法
JP2019016859A (ja) 2017-07-04 2019-01-31 オリンパス株式会社 観察装置および観察方法
AU2018320864B2 (en) 2017-08-22 2024-02-22 Napigen, Inc. Organelle genome modification using polynucleotide guided endonuclease
US11293019B2 (en) * 2017-12-22 2022-04-05 Gflas Life Sciences, Inc. Chimeric genome engineering molecules and methods
US11268092B2 (en) 2018-01-12 2022-03-08 GenEdit, Inc. Structure-engineered guide RNA
AU2020242032A1 (en) 2019-03-19 2021-10-07 Massachusetts Institute Of Technology Methods and compositions for editing nucleotide sequences
WO2021081367A1 (en) 2019-10-23 2021-04-29 Pairwise Plants Services, Inc. Compositions and methods for rna-templated editing in plants
BR112022008415A2 (pt) 2019-11-05 2022-09-06 Pairwise Plants Services Inc Composições e métodos para substituição do dna codificado pelo rna dos alelos
KR20230106633A (ko) 2020-11-06 2023-07-13 페어와이즈 플랜츠 서비시즈, 인크. 대립유전자의 rna-코딩된 dna-대체를 위한 조성물 및 방법

Also Published As

Publication number Publication date
US11926834B2 (en) 2024-03-12
US20240167045A1 (en) 2024-05-23
JP2023505600A (ja) 2023-02-09
EP4054621A1 (en) 2022-09-14
US20240158804A1 (en) 2024-05-16
AU2020380319A1 (en) 2022-05-12
US20210130835A1 (en) 2021-05-06
CN114901302A (zh) 2022-08-12
BR112022008415A2 (pt) 2022-09-06
EP4054621A4 (en) 2023-12-27
WO2021092130A1 (en) 2021-05-14
KR20220110748A (ko) 2022-08-09
MX2022005328A (es) 2022-07-21
CA3160186A1 (en) 2021-05-14
IL292434A (en) 2022-06-01

Similar Documents

Publication Publication Date Title
CL2022001145A1 (es) Composiciones y métodos para el reemplazo de alelos de adn codificado por arn
CL2021002740A1 (es) Una proteína crispr de prevotella y francisella 1 (cpf1) aislada de acidaminococcus sp. bv3l6 (ascpf1); proteína de fusión que la comprende; ácido nucleico; vector; métodos para alterar el genoma de una célula o una molécula de adnbc in vitro que utilizan dichas proteínas (divisional de sol. 201903004)
EA201892810A1 (ru) Гибридные последовательности нуклеиновых кислот для геномной инженерии
CL2023001284A1 (es) Composiciones y métodos para el reemplazo de alelos con adn codificado por arn
BR112022021789A2 (pt) Bibliotecas de ácido nucléico variantes para coronavírus
BR112018076190A2 (pt) ortólogos e sistemas crispr tipo vi
WO2020086144A3 (en) APPLICATIONS OF CRISPRi IN HIGH THROUGHPUT METABOLIC ENGINEERING
CO2018004763A2 (es) Edición genómica multiplexada
PE20211402A1 (es) Metodos y composiciones para editar acidos ribonucleicos
EA201991443A1 (ru) ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
BR112021020608A2 (pt) Rna recrutador de desaminase, construção, método para editar um rna alvo em uma célula hospedeira, e, construção, composição, célula, biblioteca ou kit
BR112022011235A2 (pt) Bibliotecas de variantes de ácido nucleico para receptores de adenosina
MX2022011562A (es) Métodos y composiciones para edición dirigida de genoma.
BR112022009670A2 (pt) Polipeptídeos efetores de crispr-cas e métodos de uso dos mesmos
EP4253551A3 (en) Novel crispr dna and rna targeting enzymes and systems
DE50311850D1 (de) Konstrukte und verfahren zur regulation der genexpression
US20060147990A1 (en) Process for producing template DNA and process for producing protein in cell-free protein synthesis system with the use of the same
BR112022015969A2 (pt) Composições e métodos para enxerto de células editadas de base
CL2023000554A1 (es) Proteínas diseñadas y métodos de uso de las mismas.
WO2023049872A3 (en) Self-inactivating vectors for gene editing
CO2017009267A2 (es) Proteína resistente a herbicidas, gen codificante, método para producir una proteína resistente a los herbicidas, método para seleccionar células vegetales transformadas y proteger las plantas del daño causado por los herbicidas
BR112022010321A2 (pt) Composições e métodos para tratamento de neuropatia óptica hereditária de leber com proteínas nadh desidrogenase
BR112022008861A2 (pt) Construto de ácido nucleico, vetor, nanopartícula lipídica, célula, composição para uso na expressão de retinosquisina, método para integrar uma sequência de codificação, e, método para modificar um gene rs1
WO2019229064A3 (en) Method for producing albicanol compounds
EP4343770A3 (en) Methods and uses of introducing mutations into genetic material for genome assembly