CL2023001284A1 - Composiciones y métodos para el reemplazo de alelos con adn codificado por arn - Google Patents

Composiciones y métodos para el reemplazo de alelos con adn codificado por arn

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Publication number
CL2023001284A1
CL2023001284A1 CL2023001284A CL2023001284A CL2023001284A1 CL 2023001284 A1 CL2023001284 A1 CL 2023001284A1 CL 2023001284 A CL2023001284 A CL 2023001284A CL 2023001284 A CL2023001284 A CL 2023001284A CL 2023001284 A1 CL2023001284 A1 CL 2023001284A1
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CL
Chile
Prior art keywords
methods
rna
compositions
encoded dna
allele replacement
Prior art date
Application number
CL2023001284A
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English (en)
Inventor
Kim Yongjoo
Matthew Watts Joseph
Hummel Aaron
Joshua Lawit Shai
Schwark David
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of CL2023001284A1 publication Critical patent/CL2023001284A1/es

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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    • C12N9/14—Hydrolases (3)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)

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  • Biochemistry (AREA)
  • Microbiology (AREA)
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  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
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  • Gastroenterology & Hepatology (AREA)
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  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Esta invención se refiere a construcciones nucleicas recombinantes que comprenden proteínas efectoras CRISPR-Cas, transcriptasas inversas y ácidos nucleicos guía extendidos y métodos de uso de los mismos para modificar ácidos nucleicos en plantas.
CL2023001284A 2020-11-06 2023-05-03 Composiciones y métodos para el reemplazo de alelos con adn codificado por arn CL2023001284A1 (es)

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US202063110386P 2020-11-06 2020-11-06

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CL2023001284A1 true CL2023001284A1 (es) 2023-12-22

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US (1) US20220145334A1 (es)
EP (1) EP4240844A2 (es)
JP (1) JP2023549339A (es)
KR (1) KR20230106633A (es)
CN (1) CN116745418A (es)
AU (1) AU2021374974A1 (es)
CA (1) CA3200521A1 (es)
CL (1) CL2023001284A1 (es)
IL (1) IL302526A (es)
MX (1) MX2023005150A (es)
WO (1) WO2022098993A2 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3160186A1 (en) 2019-11-05 2021-05-14 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
US20220403475A1 (en) 2021-06-14 2022-12-22 Pairwise Plants Services, Inc. Reporter constructs, compositions comprising the same, and methods of use thereof
US20230266293A1 (en) 2021-09-21 2023-08-24 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
US20230287441A1 (en) * 2021-12-17 2023-09-14 Massachusetts Institute Of Technology Programmable insertion approaches via reverse transcriptase recruitment

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CA3160186A1 (en) * 2019-11-05 2021-05-14 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles

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KR20230106633A (ko) 2023-07-13
CA3200521A1 (en) 2022-05-12
WO2022098993A2 (en) 2022-05-12
AU2021374974A1 (en) 2023-05-25
MX2023005150A (es) 2023-05-26
WO2022098993A3 (en) 2022-06-16
EP4240844A2 (en) 2023-09-13
CN116745418A (zh) 2023-09-12
IL302526A (en) 2023-07-01
US20220145334A1 (en) 2022-05-12
JP2023549339A (ja) 2023-11-24

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