CO4810250A1 - Promotores de plastidos clp para la expresion transgenica en los plastidos de plantas superiores - Google Patents

Promotores de plastidos clp para la expresion transgenica en los plastidos de plantas superiores

Info

Publication number
CO4810250A1
CO4810250A1 CO98031385A CO98031385A CO4810250A1 CO 4810250 A1 CO4810250 A1 CO 4810250A1 CO 98031385 A CO98031385 A CO 98031385A CO 98031385 A CO98031385 A CO 98031385A CO 4810250 A1 CO4810250 A1 CO 4810250A1
Authority
CO
Colombia
Prior art keywords
promoters
higher plants
plastide
plastides
clp
Prior art date
Application number
CO98031385A
Other languages
English (en)
Inventor
Maliga Pal
Daniel Silhavy
Sriraman Priya
Original Assignee
Univ Rutgers
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rutgers filed Critical Univ Rutgers
Publication of CO4810250A1 publication Critical patent/CO4810250A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención provee nuevas construcciones de ADN para transformar establemente los plástidos de plantas superiores. Las construcciones aquí descritas contienen promotores únicos que son transcritos tanto por ARN polimerasa de plástidos nucleares codificados, como por ARN polimerasa de plástidos de plástidos codificados, o ambos. El uso de las nuevas construcciones de la invención facilita la transformación de un rango más amplio de especies de plantas y permite la expresión ubicua de un ADN de transformación en plástidos de plantas multicelulares.
CO98031385A 1997-06-03 1998-06-03 Promotores de plastidos clp para la expresion transgenica en los plastidos de plantas superiores CO4810250A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4837697P 1997-06-03 1997-06-03
US5867097P 1997-09-12 1997-09-12

Publications (1)

Publication Number Publication Date
CO4810250A1 true CO4810250A1 (es) 1999-06-30

Family

ID=26726065

Family Applications (1)

Application Number Title Priority Date Filing Date
CO98031385A CO4810250A1 (es) 1997-06-03 1998-06-03 Promotores de plastidos clp para la expresion transgenica en los plastidos de plantas superiores

Country Status (10)

Country Link
US (2) US6624296B1 (es)
EP (1) EP1015557B1 (es)
JP (1) JP2002502262A (es)
AR (1) AR012911A1 (es)
AT (1) ATE468389T1 (es)
AU (1) AU7812598A (es)
CA (1) CA2292782C (es)
CO (1) CO4810250A1 (es)
DE (1) DE69841673D1 (es)
WO (1) WO1998055595A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR200002503T2 (tr) * 1998-03-11 2000-12-21 Novartis Ag Yeni bitki plastid promotör sekansı
AU6277600A (en) * 1999-07-27 2001-02-13 Syngenta Participations Ag Novel chimeric genes
US20040253586A1 (en) * 1999-12-08 2004-12-16 Siva Reddy Plastid transformation
US20050198704A1 (en) * 2000-10-20 2005-09-08 Board Of Trustees Of Michigan State University Chloroplast transgenesis of monocots: bioconfined genetically engineered monocot crops that will eliminate transgene flow
JP4838434B2 (ja) * 2001-03-22 2011-12-14 財団法人地球環境産業技術研究機構 植物体を用いたタンパク質発現系
US7786344B2 (en) 2002-07-26 2010-08-31 Basf Plant Science Gmbh Selection method
KR20050054928A (ko) * 2002-09-27 2005-06-10 센트로 데 인제니에리아 제네티카 와이 바이오테크놀로지아 피자식물의 유전자 형질전환 식물의 생산용 벡터
US7176355B2 (en) * 2002-12-13 2007-02-13 Rutgers, The State University Of New Jersey Plastid rRNA operon promoter elements for construction of chimeric promoters for transgene expression
KR100740694B1 (ko) * 2006-02-09 2007-07-18 한국생명공학연구원 색소체 게놈 내에 삽입된 외래유전자의 이차 재조합을방지하기 위한 벡터개발
KR100813150B1 (ko) * 2006-11-07 2008-03-17 한국생명공학연구원 Ｍｄｈ 유전자의 색소체 형질전환을 통한 식물체의광합성량 또는 바이오매스 증대 방법
US20100273854A1 (en) * 2007-06-15 2010-10-28 Hagar Kalinski Compositions and methods for inhibiting nadph oxidase expression
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
EP3260542A1 (en) 2016-06-20 2017-12-27 Algentech Protein production in plant cells
CN113025615B (zh) * 2021-04-07 2022-07-29 武汉天问生物科技有限公司 一种非绿色组织高效表达顺式元件及其应用
EP4426842A1 (en) 2021-11-01 2024-09-11 Flagship Pioneering Innovations VII, LLC Polynucleotides for modifying organisms

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6394929A (ja) 1986-06-23 1988-04-26 バイオテクニカ・インタ−ナショナル・インコ−ポレ−テッド 葉緑体の形質転換
US5877402A (en) 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
CA2225652C (en) * 1995-08-10 2007-11-20 Pal Maliga Nuclear-encoded transcription system in plastids of higher plants

Also Published As

Publication number Publication date
JP2002502262A (ja) 2002-01-22
EP1015557A4 (en) 2005-03-09
US7452986B2 (en) 2008-11-18
CA2292782A1 (en) 1998-12-10
WO1998055595A1 (en) 1998-12-10
AR012911A1 (es) 2000-11-22
ATE468389T1 (de) 2010-06-15
US20040040058A1 (en) 2004-02-26
EP1015557A1 (en) 2000-07-05
US6624296B1 (en) 2003-09-23
CA2292782C (en) 2012-01-10
EP1015557B1 (en) 2010-05-19
DE69841673D1 (de) 2010-07-01
AU7812598A (en) 1998-12-21

Similar Documents

Publication Publication Date Title
CO4810250A1 (es) Promotores de plastidos clp para la expresion transgenica en los plastidos de plantas superiores
AR022895A1 (es) Composiciones y metodos para la modificacion de la transcripcion genica
BR9609896A (pt) Construção de dna para transformar estavelmente plastídeos de plantas multicelulares e de uma célula de plantas e para expressão de pelo menos um produto de gene adicional na mesma planta multicelular e processo para obter uma célula de planta ou uma planta multicelular
AR066201A2 (es) Polinucleotidos optimizados para la expresion en plantas que codifican proteinas pesticidas, y metodo para controlar una plaga de plantas
AR026994A1 (es) Nuevas construcciones de expresion en plantas.
GT200100007A (es) Antagonistas del factor liberador de corticotropina.
AR017573A1 (es) Hidroxifenilpiruvato-dioxigenasa mutada, secuencia de adn y obtencion de plantas que contienen un gen tal, tolerantes a los herbicidas
AR032709A1 (es) Procedimiento para identificar sustancias con accion herbicida
AR065242A1 (es) Promotores y elementos reguladores de genes vegetales de tipo mtn3 inducibles por nematodos
ES2052588T3 (es) Celulas de plantas preparadas por ingenieria genetica, que muestran resistencia a los inhibidores de la glutamina sintetasa.
AR053893A1 (es) Plantas con caracteristicas de crecimiento mejorado y metodo para desarrollar las mismas
CL2023001284A1 (es) Composiciones y métodos para el reemplazo de alelos con adn codificado por arn
AR050804A1 (es) Moleculas promotoras para uso en plantas
CL2020002249A1 (es) Elementos regulatorios de plantas y sus usos (divisional 201902034)
AR021056A1 (es) Adn que comprende un gen especifico de antera de arroz y planta transgenica transformada con el mismo
BR0212339A (pt) promotor 7s alfa especìfico para semente na expressão de genes em plantas
AR049412A1 (es) Procedimiento para incrementar la resistencia a los agentes patogenos en plantas transgenicas por expresion de una peroxidasa
BR9809091A (pt) Marcador de seleção
CR20180481A (es) Elementos reguladores vegetales y sus usos
AR024934A1 (es) Genes quimericos
BR0309870A (pt) Composições e métodos para a modificação de expressão de genes usando o promotor de superubiquitina de pinus radiata
BR0008035A (pt) Composições isoladas de células de planta e seu uso na modificação de sinalização de célula de planta
AR032686A1 (es) Elementos reguladores adecuados para usar en la expresion genetica
BR0010396A (pt) Composições e métodos para a modificação da expressão de genes
CL2021000262A1 (es) Elementos reguladores de plantas y usos de los mismos