AR012911A1 - UNA CONSTRUCCION DE ACIDO NUCLEICO CON UN PROMOTOR MEJORADO (clpP) DEL GEN QUE CODIFICA UNA PROTEASA DEPENDIENTE DE ATP, UN VECTOR QUE LA CONTIENE YUN METODO QUE EMPLEA DICHA CONSTRUCCION PARA EXPRESAR ESTABLEMENTE UNA PROTEINA EXOGENA EN LOS PLASTIDOS DE PLANTAS MONOCOTILEDONEAS Y DICOTILEDONEAS - Google Patents

UNA CONSTRUCCION DE ACIDO NUCLEICO CON UN PROMOTOR MEJORADO (clpP) DEL GEN QUE CODIFICA UNA PROTEASA DEPENDIENTE DE ATP, UN VECTOR QUE LA CONTIENE YUN METODO QUE EMPLEA DICHA CONSTRUCCION PARA EXPRESAR ESTABLEMENTE UNA PROTEINA EXOGENA EN LOS PLASTIDOS DE PLANTAS MONOCOTILEDONEAS Y DICOTILEDONEAS

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Publication number
AR012911A1
AR012911A1 ARP980102589A ARP980102589A AR012911A1 AR 012911 A1 AR012911 A1 AR 012911A1 AR P980102589 A ARP980102589 A AR P980102589A AR P980102589 A ARP980102589 A AR P980102589A AR 012911 A1 AR012911 A1 AR 012911A1
Authority
AR
Argentina
Prior art keywords
construction
clpp
atp
nucleic acid
protein
Prior art date
Application number
ARP980102589A
Other languages
English (en)
Original Assignee
Univ Rutgers
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rutgers filed Critical Univ Rutgers
Publication of AR012911A1 publication Critical patent/AR012911A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Una construccion de ácido nucleico con un promotor mejorado (clpP) del gen que codifica una proteasa dependiente de AtP (clpP), un vector que lacontiene y un método que emplea dicha construccion para expresar establemente una proteína exogena en los plástidos de plantas monocotiledomeasy dicotiledoneas. Las construcciones descriptas en este documento contienen promotores unicos que se transcriben tanto por medio de las polimerasas deARN de plástidos codificadas en el nucleo como por medio de las polimerasas de ARN de plástidos codificadas en los plástidos, como por ambas. Las nuevasconstrucciones son insertadas en vectores y se emplean en un método para la transformacion de una amplia gama de especies de plantasy posibilita laexpresion ubiquitaria de un ADN dentro de los plástidos de las plantas multicelulares.
ARP980102589A 1997-06-03 1998-06-03 UNA CONSTRUCCION DE ACIDO NUCLEICO CON UN PROMOTOR MEJORADO (clpP) DEL GEN QUE CODIFICA UNA PROTEASA DEPENDIENTE DE ATP, UN VECTOR QUE LA CONTIENE YUN METODO QUE EMPLEA DICHA CONSTRUCCION PARA EXPRESAR ESTABLEMENTE UNA PROTEINA EXOGENA EN LOS PLASTIDOS DE PLANTAS MONOCOTILEDONEAS Y DICOTILEDONEAS AR012911A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4837697P 1997-06-03 1997-06-03
US5867097P 1997-09-12 1997-09-12

Publications (1)

Publication Number Publication Date
AR012911A1 true AR012911A1 (es) 2000-11-22

Family

ID=26726065

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980102589A AR012911A1 (es) 1997-06-03 1998-06-03 UNA CONSTRUCCION DE ACIDO NUCLEICO CON UN PROMOTOR MEJORADO (clpP) DEL GEN QUE CODIFICA UNA PROTEASA DEPENDIENTE DE ATP, UN VECTOR QUE LA CONTIENE YUN METODO QUE EMPLEA DICHA CONSTRUCCION PARA EXPRESAR ESTABLEMENTE UNA PROTEINA EXOGENA EN LOS PLASTIDOS DE PLANTAS MONOCOTILEDONEAS Y DICOTILEDONEAS

Country Status (10)

Country Link
US (2) US6624296B1 (es)
EP (1) EP1015557B1 (es)
JP (1) JP2002502262A (es)
AR (1) AR012911A1 (es)
AT (1) ATE468389T1 (es)
AU (1) AU7812598A (es)
CA (1) CA2292782C (es)
CO (1) CO4810250A1 (es)
DE (1) DE69841673D1 (es)
WO (1) WO1998055595A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR200002503T2 (tr) * 1998-03-11 2000-12-21 Novartis Ag Yeni bitki plastid promotör sekansı
AU6277600A (en) * 1999-07-27 2001-02-13 Syngenta Participations Ag Novel chimeric genes
US20040253586A1 (en) * 1999-12-08 2004-12-16 Siva Reddy Plastid transformation
US20050198704A1 (en) * 2000-10-20 2005-09-08 Board Of Trustees Of Michigan State University Chloroplast transgenesis of monocots: bioconfined genetically engineered monocot crops that will eliminate transgene flow
JP4838434B2 (ja) * 2001-03-22 2011-12-14 財団法人地球環境産業技術研究機構 植物体を用いたタンパク質発現系
US7786344B2 (en) 2002-07-26 2010-08-31 Basf Plant Science Gmbh Selection method
KR20050054928A (ko) * 2002-09-27 2005-06-10 센트로 데 인제니에리아 제네티카 와이 바이오테크놀로지아 피자식물의 유전자 형질전환 식물의 생산용 벡터
US7176355B2 (en) * 2002-12-13 2007-02-13 Rutgers, The State University Of New Jersey Plastid rRNA operon promoter elements for construction of chimeric promoters for transgene expression
KR100740694B1 (ko) * 2006-02-09 2007-07-18 한국생명공학연구원 색소체 게놈 내에 삽입된 외래유전자의 이차 재조합을방지하기 위한 벡터개발
KR100813150B1 (ko) * 2006-11-07 2008-03-17 한국생명공학연구원 Ｍｄｈ 유전자의 색소체 형질전환을 통한 식물체의광합성량 또는 바이오매스 증대 방법
US20100273854A1 (en) * 2007-06-15 2010-10-28 Hagar Kalinski Compositions and methods for inhibiting nadph oxidase expression
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
EP3260542A1 (en) 2016-06-20 2017-12-27 Algentech Protein production in plant cells
CN113025615B (zh) * 2021-04-07 2022-07-29 武汉天问生物科技有限公司 一种非绿色组织高效表达顺式元件及其应用
EP4426842A1 (en) 2021-11-01 2024-09-11 Flagship Pioneering Innovations VII, LLC Polynucleotides for modifying organisms

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6394929A (ja) 1986-06-23 1988-04-26 バイオテクニカ・インタ−ナショナル・インコ−ポレ−テッド 葉緑体の形質転換
US5877402A (en) 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
CA2225652C (en) * 1995-08-10 2007-11-20 Pal Maliga Nuclear-encoded transcription system in plastids of higher plants

Also Published As

Publication number Publication date
JP2002502262A (ja) 2002-01-22
EP1015557A4 (en) 2005-03-09
US7452986B2 (en) 2008-11-18
CA2292782A1 (en) 1998-12-10
WO1998055595A1 (en) 1998-12-10
CO4810250A1 (es) 1999-06-30
ATE468389T1 (de) 2010-06-15
US20040040058A1 (en) 2004-02-26
EP1015557A1 (en) 2000-07-05
US6624296B1 (en) 2003-09-23
CA2292782C (en) 2012-01-10
EP1015557B1 (en) 2010-05-19
DE69841673D1 (de) 2010-07-01
AU7812598A (en) 1998-12-21

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