JP7182545B2 - 熱安定性cas9ヌクレアーゼ - Google Patents

熱安定性cas9ヌクレアーゼ Download PDF

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JP7182545B2
JP7182545B2 JP2019533096A JP2019533096A JP7182545B2 JP 7182545 B2 JP7182545 B2 JP 7182545B2 JP 2019533096 A JP2019533096 A JP 2019533096A JP 2019533096 A JP2019533096 A JP 2019533096A JP 7182545 B2 JP7182545 B2 JP 7182545B2
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nucleic acid
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protein
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seq
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JP2020504603A (ja
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デル ウースト,ジョン ファン
クラーネンブルク,リチャード ファン
ボスマ,エレッケ,フェンナ
モウギアコス,イオアニス
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ヴァーヘニンゲン ユニヴェルシテット
スティッチング フォール デ テクニッシュ ウェテンスシャペン
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    • C12N2750/14141Use of virus, viral particle or viral elements as a vector
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JP2019533096A 2016-12-14 2016-12-14 熱安定性cas9ヌクレアーゼ Active JP7182545B2 (ja)

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PCT/EP2016/081077 WO2018108272A1 (en) 2016-12-14 2016-12-14 Thermostable cas9 nucleases

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JP2020504603A JP2020504603A (ja) 2020-02-13
JP7182545B2 true JP7182545B2 (ja) 2022-12-02

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JP2019533096A Active JP7182545B2 (ja) 2016-12-14 2016-12-14 熱安定性cas9ヌクレアーゼ
JP2019533103A Active JP7223377B2 (ja) 2016-12-14 2017-08-16 熱安定性cas9ヌクレアーゼ
JP2019532936A Active JP7223376B2 (ja) 2016-12-14 2017-08-16 熱安定性cas9ヌクレアーゼ
JP2019533098A Active JP7283698B2 (ja) 2016-12-14 2017-12-14 熱安定性cas9ヌクレアーゼ
JP2022121297A Withdrawn JP2022166045A (ja) 2016-12-14 2022-07-29 熱安定性cas9ヌクレアーゼ
JP2022128902A Withdrawn JP2022166170A (ja) 2016-12-14 2022-08-12 熱安定性cas9ヌクレアーゼ

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JP2019533103A Active JP7223377B2 (ja) 2016-12-14 2017-08-16 熱安定性cas9ヌクレアーゼ
JP2019532936A Active JP7223376B2 (ja) 2016-12-14 2017-08-16 熱安定性cas9ヌクレアーゼ
JP2019533098A Active JP7283698B2 (ja) 2016-12-14 2017-12-14 熱安定性cas9ヌクレアーゼ
JP2022121297A Withdrawn JP2022166045A (ja) 2016-12-14 2022-07-29 熱安定性cas9ヌクレアーゼ
JP2022128902A Withdrawn JP2022166170A (ja) 2016-12-14 2022-08-12 熱安定性cas9ヌクレアーゼ

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US (5) US11242513B2 (https=)
EP (4) EP3555275A1 (https=)
JP (6) JP7182545B2 (https=)
KR (4) KR102913446B1 (https=)
CN (4) CN110312792B (https=)
AU (4) AU2016432443B2 (https=)
BR (3) BR112019012155A2 (https=)
CA (4) CA3046824A1 (https=)
EA (4) EA201991443A1 (https=)
PH (4) PH12019501344A1 (https=)
WO (4) WO2018108272A1 (https=)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9637739B2 (en) * 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP4464338A3 (en) 2014-11-07 2025-02-12 Editas Medicine, Inc. Systems for improving crispr/cas-mediated genome-editing
CA2986310A1 (en) 2015-05-11 2016-11-17 Editas Medicine, Inc. Optimized crispr/cas9 systems and methods for gene editing in stem cells
CN108026526B (zh) 2015-06-09 2023-05-12 爱迪塔斯医药公司 用于改善移植的crispr/cas相关方法和组合物
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
EP3353296B1 (en) 2015-09-24 2020-11-04 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
US11597924B2 (en) 2016-03-25 2023-03-07 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
BR112019012155A2 (pt) 2016-12-14 2019-11-12 Wageningen Universiteit uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína
EP3555297A1 (en) 2016-12-19 2019-10-23 Editas Medicine, Inc. Assessing nuclease cleavage
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
JP7229923B2 (ja) 2017-01-06 2023-02-28 エディタス・メディシン、インコーポレイテッド ヌクレアーゼ切断を評価する方法
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
US11499151B2 (en) 2017-04-28 2022-11-15 Editas Medicine, Inc. Methods and systems for analyzing guide RNA molecules
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
MX2019014640A (es) 2017-06-09 2020-10-05 Editas Medicine Inc Nucleasas de repeticiones palíndromicas agrupadas y regularmente interespaciadas de proteina asociada 9 (cas9) genomanipuladas.
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
GB201716590D0 (en) * 2017-10-10 2017-11-22 Univ Wageningen Thermostable cas9 nucleases with reduced off-target activity
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
KR102465067B1 (ko) 2018-02-15 2022-11-10 시그마-알드리치 컴퍼니., 엘엘씨 진핵 게놈 변형을 위한 조작된 cas9 시스템
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
EP3823633A4 (en) 2018-06-29 2023-05-03 Editas Medicine, Inc. SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
EP3874048A1 (en) 2018-11-01 2021-09-08 Keygene N.V. Dual guide rna for crispr/cas genome editing in plants cells
CN113330122A (zh) * 2018-11-16 2021-08-31 德皮克斯公司 使用位点特异性核酸酶优化核酸的体外分离
RU2712497C1 (ru) * 2018-11-26 2020-01-29 Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий Средство разрезания ДНК на основе Cas9 белка из биотехнологически значимой бактерии Clostridium cellulolyticum
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
US10947517B2 (en) 2019-02-15 2021-03-16 Sigma-Aldrich Co. Llc CRISPR/Cas fusion proteins and systems
WO2020191233A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN116694603A (zh) * 2019-05-14 2023-09-05 深圳华大生命科学研究院 新型的Cas蛋白、Crispr-Cas系统及其在基因编辑领域中的用途
WO2021017200A1 (zh) * 2019-07-30 2021-02-04 湖北大学 一种基于运动发酵单胞菌的CRISPR-Cas系统、基因组编辑体系及其应用
CN110331158B (zh) * 2019-07-30 2021-09-14 湖北大学 基于运动发酵单胞菌内源CRISPR-Cas系统的多基因位点同时编辑方法及其应用
CN112410234B (zh) * 2019-08-21 2022-08-23 江南大学 一种多靶点编辑重组曲霉菌株的可视化筛选方法
US11879134B1 (en) * 2019-09-05 2024-01-23 The Regents Of The University Of Colorado, A Body Corporate Recombineering machinery to increase homology directed genome editing in thermophilic microbes
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CN110951739B (zh) * 2019-12-25 2021-03-30 江南大学 一种由高温诱导表达的启动子及其应用
IL297761A (en) 2020-05-08 2022-12-01 Broad Inst Inc Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence
CN111778230A (zh) * 2020-07-17 2020-10-16 山东舜丰生物科技有限公司 一种适用于Cas12蛋白的缓冲系统及其应用
RU2749307C1 (ru) * 2020-10-30 2021-06-08 Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт сельскохозяйственной биотехнологии" (ФГБНУ ВНИИСБ) Новая компактная нуклеаза CAS9 II типа из Anoxybacillus flavithermus
CN116334037A (zh) * 2020-11-11 2023-06-27 山东舜丰生物科技有限公司 新型Cas酶和系统以及应用
CN114480347B (zh) * 2022-02-21 2022-12-23 中国科学院地球化学研究所 一种纯化Cas12a蛋白的方法
CN114934031B (zh) 2022-05-25 2023-08-01 广州瑞风生物科技有限公司 新型Cas效应蛋白、基因编辑系统及用途
JP7152094B1 (ja) 2022-06-30 2022-10-12 リージョナルフィッシュ株式会社 tracrRNAユニット、及びゲノム編集方法
CN117701530A (zh) * 2022-12-08 2024-03-15 广州瑞风生物科技有限公司 Cas蛋白截短体、构建其的方法及其应用
CN116144631B (zh) * 2023-01-17 2023-09-15 华中农业大学 耐热型核酸内切酶及其介导的基因编辑系统
WO2025107617A1 (zh) * 2023-11-24 2025-05-30 华智生物技术有限公司 CRISPR/Cas效应蛋白及系统
US20250250641A1 (en) * 2024-02-06 2025-08-07 Florida State University Research Foundation, Inc. Methods and compositions related to epigenetic editing by a methlylation-dependent cas9
CN118956928B (zh) * 2024-07-08 2025-10-21 宁夏大学 一种动态调控链霉菌次生代谢过程中嗅味物质含量的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006230303A (ja) 2005-02-25 2006-09-07 Art Engineering Kk 好熱性リパーゼ産生菌およびその利用
JP2007537724A (ja) 2004-03-10 2007-12-27 イースト テネシー ステート ユニバーシティー リサーチ ファウンデーション ジオバチルス・ステアロサーモフィラス(Geobacillusstearothermophilus)由来のRNA依存型DNAポリメラーゼ
JP2010510776A (ja) 2006-11-27 2010-04-08 シージェイ チェイルジェダン コープ. Gras微生物から発現される食品安全型好熱性のアラビノース異性化酵素及びそれを用いたタガトースの製造方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2176082B1 (es) 2000-05-31 2004-01-01 Consejo Superior Investigacion Bacterias con la capacidad de unir metales pesados y su empleo en la detoxificacion de medios contaminados con metales pesados.
US7220571B2 (en) 2000-09-28 2007-05-22 Archer-Daniels-Midland Company Escherichia coli strains which over-produce L-threonine and processes for the production of L-threonine by fermentation
CN1526013A (zh) * 2001-02-23 2004-09-01 诺维信公司 脂解酶基因
EP2468869B1 (en) * 2007-01-31 2015-03-18 Pfenex Inc. Bacterial leader sequences for increased expression
AU2010300722A1 (en) 2009-09-29 2012-04-12 Butamax(Tm) Advanced Biofuels Llc Improved flux to acetolactate-derived products in lactic acid bacteria
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
EP4136963B1 (en) 2013-03-15 2026-01-21 Cibus US LLC Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
KR102450868B1 (ko) 2014-03-14 2022-10-06 시버스 유에스 엘엘씨 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물
JP6637026B2 (ja) 2014-07-23 2020-01-29 ピュラック バイオケム ビー. ブイ. 遺伝子改変された(r)−乳酸産生好熱性細菌
US10725041B2 (en) 2014-11-04 2020-07-28 Versiti Blood Research Institute Foundation, Inc. Method to bioengineer designer platelets using gene editing and stem cell methodologies
EP4464338A3 (en) 2014-11-07 2025-02-12 Editas Medicine, Inc. Systems for improving crispr/cas-mediated genome-editing
KR102424626B1 (ko) 2014-12-17 2022-07-25 이 아이 듀폰 디 네모아 앤드 캄파니 원형 폴리뉴클레오티드 변형 주형과 함께 가이드 RNA/Cas 엔도뉴클레아제 시스템을 이용하여 대장균에서 효율적으로 유전자 편집을 하기 위한 조성물 및 방법
WO2016179038A1 (en) * 2015-05-01 2016-11-10 Spark Therapeutics, Inc. ADENO-ASSOCIATED VIRUS-MEDIATED CRISPR-Cas9 TREATMENT OF OCULAR DISEASE
AU2016263026A1 (en) * 2015-05-15 2017-11-09 Pioneer Hi-Bred International, Inc. Guide RNA/Cas endonuclease systems
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
BR112019012155A2 (pt) 2016-12-14 2019-11-12 Wageningen Universiteit uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007537724A (ja) 2004-03-10 2007-12-27 イースト テネシー ステート ユニバーシティー リサーチ ファウンデーション ジオバチルス・ステアロサーモフィラス(Geobacillusstearothermophilus)由来のRNA依存型DNAポリメラーゼ
JP2006230303A (ja) 2005-02-25 2006-09-07 Art Engineering Kk 好熱性リパーゼ産生菌およびその利用
JP2010510776A (ja) 2006-11-27 2010-04-08 シージェイ チェイルジェダン コープ. Gras微生物から発現される食品安全型好熱性のアラビノース異性化酵素及びそれを用いたタガトースの製造方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Biotechnology for Biofuels,2016年10月06日,volume 9, Article number: 210,1-13
Genome Biology,2015年,16:253,1-13
Molecular Cell,2016年04月07日,vol.62,p.137-147
UniProtKB,[online],A0A178TEJ9_GEOSE,検索日[2020/12/01],2016年11月02日,<https://www.uniprot.org/uniprot/A0A178TEJ9.txt?version=3>

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