JP2019521322A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2019521322A5 JP2019521322A5 JP2018559313A JP2018559313A JP2019521322A5 JP 2019521322 A5 JP2019521322 A5 JP 2019521322A5 JP 2018559313 A JP2018559313 A JP 2018559313A JP 2018559313 A JP2018559313 A JP 2018559313A JP 2019521322 A5 JP2019521322 A5 JP 2019521322A5
- Authority
- JP
- Japan
- Prior art keywords
- bead
- buffer
- dna
- biological sample
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022033452A JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662336203P | 2016-05-13 | 2016-05-13 | |
| US62/336,203 | 2016-05-13 | ||
| PCT/US2017/032719 WO2017197399A1 (en) | 2016-05-13 | 2017-05-15 | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022033452A Division JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019521322A JP2019521322A (ja) | 2019-07-25 |
| JP2019521322A5 true JP2019521322A5 (enExample) | 2020-07-02 |
| JP7037504B2 JP7037504B2 (ja) | 2022-03-16 |
Family
ID=59014730
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018559313A Active JP7037504B2 (ja) | 2016-05-13 | 2017-05-15 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
| JP2022033452A Active JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022033452A Active JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10808240B2 (enExample) |
| EP (2) | EP4079853A1 (enExample) |
| JP (2) | JP7037504B2 (enExample) |
| CN (1) | CN109642229A (enExample) |
| AU (1) | AU2017263886B2 (enExample) |
| CA (1) | CA3022528A1 (enExample) |
| WO (1) | WO2017197399A1 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11268085B2 (en) * | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| US10808240B2 (en) | 2016-05-13 | 2020-10-20 | Exosome Diagnostics, Inc. | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids |
| KR20190100174A (ko) | 2016-12-23 | 2019-08-28 | 엑소팜 리미티드 | 미세소포 및 엑소좀의 정제 또는 단리를 위한 방법 및 조성물 |
| KR101875594B1 (ko) * | 2017-01-13 | 2018-07-06 | ㈜로제타엑소좀 | 금속을 이용한 세포밖 소포체의 분리 방법 |
| EP3635139A1 (en) | 2017-05-17 | 2020-04-15 | Exosome Diagnostics, Inc. | Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection |
| CN111148828A (zh) * | 2017-07-26 | 2020-05-12 | 罗塞塔外排体株式会社 | 利用阳离子分离细胞外囊泡的方法 |
| EP3807616A4 (en) * | 2018-06-18 | 2022-03-23 | Exopharm Limited | METHODS AND COMPOSITIONS FOR PURIFICATION OR ISOLATION OF MICROVESICLES AND EXOSOMES |
| WO2020077294A1 (en) * | 2018-10-12 | 2020-04-16 | Simpore, Inc. | Extracellular vesicle isolation by nanomembranes |
| WO2020106853A1 (en) | 2018-11-20 | 2020-05-28 | Exosome Diagnostics, Inc. | Compositions and methods for internal controls of microvesicle isolations |
| CN111254138A (zh) * | 2018-12-03 | 2020-06-09 | 北京银丰鼎诚生物工程技术有限公司 | 一种分子克隆中提高酶切产物浓缩效率的方法 |
| EP3941607A1 (en) | 2019-03-21 | 2022-01-26 | Codiak BioSciences, Inc. | Process for preparing extracellular vesicles |
| GB201906722D0 (en) * | 2019-05-13 | 2019-06-26 | Ttp Plc | A method of preparing a sample for a diagnostic assay |
| CN110106169A (zh) * | 2019-05-28 | 2019-08-09 | 浙江大学 | 一种提高无细胞体液中微量核酸捕获率的方法 |
| EP4031865A2 (en) | 2019-09-18 | 2022-07-27 | Exosome Diagnostics, Inc. | Compositions, methods, and kits for the isolation of extracellular vesicles |
| EP4077659A1 (en) | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Enrichment method |
| EP4077660A1 (en) | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Method for enriching vesicular rna |
| JP2023508411A (ja) * | 2019-12-23 | 2023-03-02 | バイオプセンス オサケユキチュア | 血液試料からセルフリー核酸を抽出するための方法、自動化システムおよびカートリッジ |
| CN115461445A (zh) * | 2020-04-15 | 2022-12-09 | 合同会社予幸集团中央研究所 | 细胞外囊泡的回收方法 |
| WO2021217173A1 (en) * | 2020-04-21 | 2021-10-28 | Siemens Healthcare Diagnostics Inc. | Clinical specimen collection swabs for molecular diagnostic applications and methods of production and use thereof |
| US20230203587A1 (en) | 2020-05-29 | 2023-06-29 | Exosome Diagnostics, Inc. | Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection |
| WO2022066934A2 (en) * | 2020-09-23 | 2022-03-31 | Codiak Biosciences, Inc. | Process for preparing extracellular vesicles |
| CZ35067U1 (cs) * | 2020-12-15 | 2021-05-18 | Univerzita Palackého v Olomouci | Sada pro izolaci RNA |
| JP7773765B2 (ja) * | 2020-12-18 | 2025-11-20 | 国立研究開発法人産業技術総合研究所 | 回収用キットおよび核酸含有溶液の調製方法 |
| US20220315992A1 (en) * | 2021-04-06 | 2022-10-06 | ID Match, LLC | Method for identification of urine drug testing sample |
| WO2022263500A1 (en) * | 2021-06-17 | 2022-12-22 | Qiagen Gmbh | Method for isolating non-vesicular mirna |
| US20250075258A1 (en) | 2021-07-16 | 2025-03-06 | Exosome Diagnostics, Inc. | Methods of detecting sjogren's syndrome using salivary exosomes |
| DE102021208893B3 (de) | 2021-08-13 | 2022-11-17 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Isolieren von Analyten unterschiedlicher Analyt-Klassen |
| SE2100130A1 (en) * | 2021-09-01 | 2023-03-02 | Frontline Diagnostics AB | System for integrated automated isolation and analysis of biological vesicles and associated molecules |
| US20250009797A1 (en) * | 2021-11-04 | 2025-01-09 | Mayo Foundation For Medical Education And Research | Methods and materials for treating cancer |
| AU2023221644A1 (en) | 2022-02-18 | 2024-09-12 | Bio-Techne Corporation | Use of microvesicle signatures in the identification and treatment of renal disorders |
| WO2024054572A1 (en) | 2022-09-07 | 2024-03-14 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| WO2024185655A1 (ja) * | 2023-03-06 | 2024-09-12 | 東ソー株式会社 | 細胞外小胞精製用磁性粒子およびその製造方法、ならびに、細胞外小胞の精製方法 |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| AU645915B2 (en) | 1991-07-23 | 1994-01-27 | F. Hoffmann-La Roche Ag | Improvements in the in situ PCR |
| US5605798A (en) | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
| US5639606A (en) | 1993-04-06 | 1997-06-17 | The University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| US5556773A (en) | 1993-08-06 | 1996-09-17 | Yourno; Joseph | Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes |
| CA2270132A1 (en) | 1996-11-06 | 1998-05-14 | Sequenom, Inc. | Dna diagnostics based on mass spectrometry |
| EP0985148A4 (en) | 1997-05-28 | 2004-03-10 | Inst Medical W & E Hall | DIAGNOSIS OF NUCLEIC ACIDS BY MASS SPECTROMETRY, MASS SEPARATION AND BASE-SPECIFIC CLEAVING |
| US6004755A (en) | 1998-04-07 | 1999-12-21 | Incyte Pharmaceuticals, Inc. | Quantitative microarray hybridizaton assays |
| FR2788780B1 (fr) | 1999-01-27 | 2001-03-30 | Ap Cells Inc | Procede de preparation de vesicules membranaires |
| GB9927320D0 (en) | 1999-11-18 | 2000-01-12 | Chiron Spa | Exosome separation |
| US6812023B1 (en) | 2000-04-27 | 2004-11-02 | Anosys, Inc. | Methods of producing membrane vesicles |
| US6913879B1 (en) | 2000-07-10 | 2005-07-05 | Telechem International Inc. | Microarray method of genotyping multiple samples at multiple LOCI |
| US6696271B2 (en) | 2001-08-23 | 2004-02-24 | The Regents Of The University Of California | Frozen tissue microarray technology for analysis of RNA, DNA, and proteins |
| WO2003023065A1 (en) | 2001-09-06 | 2003-03-20 | Syngenta Participations Ag | Dna methylation patterns |
| ES2431944T3 (es) | 2002-06-26 | 2013-11-28 | Cold Spring Harbor Laboratory | Métodos para determinar los perfiles de metilación |
| AU2003267065A1 (en) | 2002-09-02 | 2004-03-19 | Pamgene B.V. | Novel integrated microarray analysis |
| US7141371B2 (en) | 2002-09-06 | 2006-11-28 | State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Methods for detecting and localizing DNA mutations by microarray |
| US7527929B2 (en) | 2004-07-30 | 2009-05-05 | Agencourt Bioscience Corporation | Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers |
| EP1869221A2 (en) | 2005-04-15 | 2007-12-26 | Cedars-Sinai Medical Center | Flow-cytometric heteroduplex analysis for detection of genetic alterations |
| US20060286557A1 (en) * | 2005-06-15 | 2006-12-21 | Basehore Lee S | Combined lysis and PCR buffer |
| US9428746B2 (en) * | 2007-10-31 | 2016-08-30 | Akonni Biosystems, Inc. | Method and kit for purifying nucleic acids |
| WO2011009104A1 (en) * | 2009-07-16 | 2011-01-20 | The General Hospital Corporation | Nucleic acid analysis |
| EP2245199B1 (en) | 2008-02-01 | 2013-11-13 | The General Hospital Corporation | Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions |
| US10767214B2 (en) | 2010-10-28 | 2020-09-08 | Clinical Genomics Pty Ltd | Method of microvesicle enrichment |
| SG190450A1 (en) * | 2010-12-20 | 2013-06-28 | Agency Science Tech & Res | Method of purifying exosomes |
| MX392552B (es) * | 2013-01-03 | 2025-03-11 | Exosome Diagnostics Inc | Métodos para aislar microvesículas. |
| US10619152B2 (en) * | 2014-02-10 | 2020-04-14 | Nancy Quan | Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents |
| US11268085B2 (en) * | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| AU2015287763B2 (en) | 2014-07-09 | 2019-08-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| CN104152436B (zh) * | 2014-08-01 | 2017-12-15 | 杭州新景生物试剂开发有限公司 | Dna分离纯化方法及其试剂盒 |
| CA2965500A1 (en) * | 2014-10-24 | 2016-04-28 | Abbott Molecular Inc. | Enrichment of small nucleic acids |
| CN105112400A (zh) * | 2015-08-31 | 2015-12-02 | 臻和（北京）科技有限公司 | 一种提取游离dna的试剂盒 |
| US10808240B2 (en) * | 2016-05-13 | 2020-10-20 | Exosome Diagnostics, Inc. | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids |
-
2017
- 2017-05-15 US US16/301,176 patent/US10808240B2/en active Active
- 2017-05-15 EP EP22167997.0A patent/EP4079853A1/en active Pending
- 2017-05-15 WO PCT/US2017/032719 patent/WO2017197399A1/en not_active Ceased
- 2017-05-15 EP EP17728304.1A patent/EP3455355B1/en active Active
- 2017-05-15 CN CN201780043334.9A patent/CN109642229A/zh active Pending
- 2017-05-15 AU AU2017263886A patent/AU2017263886B2/en active Active
- 2017-05-15 JP JP2018559313A patent/JP7037504B2/ja active Active
- 2017-05-15 CA CA3022528A patent/CA3022528A1/en active Pending
-
2020
- 2020-10-19 US US17/074,040 patent/US20210238582A1/en active Pending
-
2022
- 2022-03-04 JP JP2022033452A patent/JP7354327B2/ja active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2019521322A5 (enExample) | ||
| CN100537590C (zh) | Rna提取方法、rna提取试剂及生物材料的分析方法 | |
| US8202693B2 (en) | Method of isolation of nucleic acids | |
| US20140087366A1 (en) | USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS | |
| JP2009519027A (ja) | 固定組織から生体分子を抽出する方法 | |
| CN106906207A (zh) | 核酸快速提取试剂盒及其使用方法 | |
| WO2016003689A1 (en) | Method, substrate and device for separating nucleic acids | |
| JP2014176303A (ja) | ｃＤＮＡの合成方法 | |
| Pandit et al. | Capture and direct amplification of DNA on chitosan microparticles in a single PCR-optimal solution | |
| US10597651B2 (en) | Affinity matrix and devices for isolation and purification of RNA and DNA for point of care molecular devices | |
| JP2018526021A (ja) | 血液試料からの核酸精製のための組成物及び方法 | |
| ES2341959T3 (es) | Metodo para aislar acidos nucleicos. | |
| WO2007052765A1 (ja) | Rnaの抽出方法及びrnaの検出方法 | |
| US8415467B2 (en) | Method and materials for separating nucleic acid materials | |
| JP2007509620A (ja) | 迅速かつ低コストな核酸の単離方法 | |
| CN104560959A (zh) | 一种饱和氯化钠法快速批量提取血液dna的试剂盒和方法 | |
| JP2010527442A (ja) | 差次的抽出のための方法および系 | |
| US6699986B2 (en) | Electrophoretic separation of nucleic acids from proteins at low ph | |
| JP2009065849A (ja) | 核酸の抽出方法 | |
| US20230203476A1 (en) | Nucleic acid extraction method and application | |
| US20250019757A1 (en) | Purification chemistries and formats for sanger dna sequencing reactions on a micro-fluidics device | |
| CN106029910B (zh) | 对加工的生物样品储存及与之相关的改进 | |
| US20220010360A1 (en) | Multiomic analysis of cell analytes using microfluidic systems | |
| JP2014030364A (ja) | Ｄｎａの抽出方法 | |
| WO2016003809A1 (en) | Methods for amplifying nucleic acids on substrates |