JP2019521322A5 - - Google Patents
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- JP2019521322A5 JP2019521322A5 JP2018559313A JP2018559313A JP2019521322A5 JP 2019521322 A5 JP2019521322 A5 JP 2019521322A5 JP 2018559313 A JP2018559313 A JP 2018559313A JP 2018559313 A JP2018559313 A JP 2018559313A JP 2019521322 A5 JP2019521322 A5 JP 2019521322A5
- Authority
- JP
- Japan
- Prior art keywords
- bead
- buffer
- dna
- biological sample
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022033452A JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662336203P | 2016-05-13 | 2016-05-13 | |
| US62/336,203 | 2016-05-13 | ||
| PCT/US2017/032719 WO2017197399A1 (en) | 2016-05-13 | 2017-05-15 | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022033452A Division JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019521322A JP2019521322A (ja) | 2019-07-25 |
| JP2019521322A5 true JP2019521322A5 (enExample) | 2020-07-02 |
| JP7037504B2 JP7037504B2 (ja) | 2022-03-16 |
Family
ID=59014730
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018559313A Active JP7037504B2 (ja) | 2016-05-13 | 2017-05-15 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
| JP2022033452A Active JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022033452A Active JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US10808240B2 (enExample) |
| EP (2) | EP4079853A1 (enExample) |
| JP (2) | JP7037504B2 (enExample) |
| CN (1) | CN109642229A (enExample) |
| AU (1) | AU2017263886B2 (enExample) |
| WO (1) | WO2017197399A1 (enExample) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11268085B2 (en) * | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| JP7037504B2 (ja) | 2016-05-13 | 2022-03-16 | エクソサム ダイアグノスティクス，インコーポレイティド | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
| KR20190100174A (ko) | 2016-12-23 | 2019-08-28 | 엑소팜 리미티드 | 미세소포 및 엑소좀의 정제 또는 단리를 위한 방법 및 조성물 |
| KR101875594B1 (ko) * | 2017-01-13 | 2018-07-06 | ㈜로제타엑소좀 | 금속을 이용한 세포밖 소포체의 분리 방법 |
| WO2018213392A1 (en) | 2017-05-17 | 2018-11-22 | Exosome Diagnostics, Inc. | Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection |
| JP2020528766A (ja) * | 2017-07-26 | 2020-10-01 | ロゼッタ エクソソーム | 陽イオンを利用した細胞外小胞体の分離方法 |
| SG11202003588QA (en) * | 2017-09-20 | 2020-05-28 | Molecular Stethoscope Inc | Methods and systems for detecting tissue conditions |
| CN112585446A (zh) * | 2018-06-18 | 2021-03-30 | 埃克波菲尔有限公司 | 用于纯化或分离微泡和外泌体的方法与组合物 |
| US11119013B2 (en) * | 2018-10-12 | 2021-09-14 | Simpore Inc. | Extracellular vesicle isolation by nanomembranes |
| EP3884045B1 (en) | 2018-11-20 | 2023-01-11 | Exosome Diagnostics, Inc. | Methods for internal controls of microvesicle isolations |
| EP3887826A1 (en) | 2018-11-30 | 2021-10-06 | Corning Incorporated | System and method for analyzing extracellular vesicles with an optical biosensor |
| CN111254138A (zh) * | 2018-12-03 | 2020-06-09 | 北京银丰鼎诚生物工程技术有限公司 | 一种分子克隆中提高酶切产物浓缩效率的方法 |
| EP3941607A1 (en) | 2019-03-21 | 2022-01-26 | Codiak BioSciences, Inc. | Process for preparing extracellular vesicles |
| GB201906722D0 (en) * | 2019-05-13 | 2019-06-26 | Ttp Plc | A method of preparing a sample for a diagnostic assay |
| US12493029B2 (en) | 2019-09-18 | 2025-12-09 | Exosome Diagnostics, Inc. | Compositions, methods, and kits for the isolation of extracellular vesicles |
| WO2021122846A1 (en) | 2019-12-16 | 2021-06-24 | Qiagen Gmbh | Enrichment method |
| CN114761556A (zh) * | 2019-12-16 | 2022-07-15 | 凯杰有限公司 | 富集囊泡rna的方法 |
| WO2021130415A1 (en) * | 2019-12-23 | 2021-07-01 | Biopsense Oy | Method, automated system and cartridge for extraction of cell-free nucleic acids from a blood sample |
| WO2021210622A1 (ja) * | 2020-04-15 | 2021-10-21 | 合同会社Ｈ．Ｕ．グループ中央研究所 | 細胞外小胞の回収方法 |
| WO2021217173A1 (en) * | 2020-04-21 | 2021-10-28 | Siemens Healthcare Diagnostics Inc. | Clinical specimen collection swabs for molecular diagnostic applications and methods of production and use thereof |
| AU2021281287A1 (en) | 2020-05-29 | 2023-01-19 | Bio-Techne Corporation | Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection |
| US20230366794A1 (en) * | 2020-09-23 | 2023-11-16 | Codiak Biosciences, Inc. | Process for preparing extracellular vesicles |
| CZ35067U1 (cs) * | 2020-12-15 | 2021-05-18 | Univerzita Palackého v Olomouci | Sada pro izolaci RNA |
| JP7773765B2 (ja) * | 2020-12-18 | 2025-11-20 | 国立研究開発法人産業技術総合研究所 | 回収用キットおよび核酸含有溶液の調製方法 |
| US20220315992A1 (en) * | 2021-04-06 | 2022-10-06 | ID Match, LLC | Method for identification of urine drug testing sample |
| EP4355873A1 (en) * | 2021-06-17 | 2024-04-24 | QIAGEN GmbH | Method for isolating non-vesicular mirna |
| US20250075258A1 (en) | 2021-07-16 | 2025-03-06 | Exosome Diagnostics, Inc. | Methods of detecting sjogren's syndrome using salivary exosomes |
| DE102021208893B3 (de) | 2021-08-13 | 2022-11-17 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Isolieren von Analyten unterschiedlicher Analyt-Klassen |
| SE2100130A1 (en) * | 2021-09-01 | 2023-03-02 | Frontline Diagnostics AB | System for integrated automated isolation and analysis of biological vesicles and associated molecules |
| AU2022379949A1 (en) * | 2021-11-04 | 2024-05-02 | Mayo Foundation For Medical Education And Research | Methods and materials for treating cancer |
| WO2023158869A1 (en) | 2022-02-18 | 2023-08-24 | Exosome Diagnostics, Inc. | Use of microvesicle signatures in the identification and treatment of renal disorders |
| US20260022342A1 (en) * | 2022-05-24 | 2026-01-22 | FUJIFILM Holdings America Corporation | Methods and systems for developing media for extracellular vesicle production |
| WO2024054572A1 (en) | 2022-09-07 | 2024-03-14 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| JPWO2024185655A1 (enExample) * | 2023-03-06 | 2024-09-12 | ||
| WO2026036140A1 (en) * | 2024-08-09 | 2026-02-12 | Castle Biosciences, Inc. | Method and kit for isolating and sequencing nucleic acid from skin samples |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| AU645915B2 (en) | 1991-07-23 | 1994-01-27 | F. Hoffmann-La Roche Ag | Improvements in the in situ PCR |
| US5605798A (en) | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
| US5639606A (en) | 1993-04-06 | 1997-06-17 | The University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| US5556773A (en) | 1993-08-06 | 1996-09-17 | Yourno; Joseph | Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes |
| CA2270132A1 (en) | 1996-11-06 | 1998-05-14 | Sequenom, Inc. | Dna diagnostics based on mass spectrometry |
| US6994960B1 (en) | 1997-05-28 | 2006-02-07 | The Walter And Eliza Hall Institute Of Medical Research | Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage |
| US6004755A (en) | 1998-04-07 | 1999-12-21 | Incyte Pharmaceuticals, Inc. | Quantitative microarray hybridizaton assays |
| FR2788780B1 (fr) | 1999-01-27 | 2001-03-30 | Ap Cells Inc | Procede de preparation de vesicules membranaires |
| GB9927320D0 (en) | 1999-11-18 | 2000-01-12 | Chiron Spa | Exosome separation |
| US6812023B1 (en) | 2000-04-27 | 2004-11-02 | Anosys, Inc. | Methods of producing membrane vesicles |
| US6913879B1 (en) | 2000-07-10 | 2005-07-05 | Telechem International Inc. | Microarray method of genotyping multiple samples at multiple LOCI |
| US6696271B2 (en) | 2001-08-23 | 2004-02-24 | The Regents Of The University Of California | Frozen tissue microarray technology for analysis of RNA, DNA, and proteins |
| WO2003023065A1 (en) | 2001-09-06 | 2003-03-20 | Syngenta Participations Ag | Dna methylation patterns |
| JP4384603B2 (ja) | 2002-06-26 | 2009-12-16 | コールド スプリング ハーバー ラボラトリー | メチル化プロファイルを測定するための方法および組成物 |
| JP2005537002A (ja) | 2002-09-02 | 2005-12-08 | パムジーン ベー．ベー． | 新規な一体化したマイクロアレイ分析 |
| US7141371B2 (en) | 2002-09-06 | 2006-11-28 | State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Methods for detecting and localizing DNA mutations by microarray |
| WO2006015326A2 (en) | 2004-07-30 | 2006-02-09 | Agencourt Bioscience Corporation | Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers |
| US20080187923A1 (en) | 2005-04-15 | 2008-08-07 | Cedars-Sinai Medical Center | Flow-Cytometric Heteroduplex Analysis for Detection of Genetic Alterations |
| US20060286557A1 (en) * | 2005-06-15 | 2006-12-21 | Basehore Lee S | Combined lysis and PCR buffer |
| US9428746B2 (en) * | 2007-10-31 | 2016-08-30 | Akonni Biosystems, Inc. | Method and kit for purifying nucleic acids |
| AU2009212543B2 (en) | 2008-02-01 | 2015-07-09 | The General Hospital Corporation | Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions |
| DK2454588T3 (en) * | 2009-07-16 | 2015-09-28 | Gen Hospital Corp | Nucleic acid |
| WO2012054975A1 (en) | 2010-10-28 | 2012-05-03 | Clinical Genomics Pty. Ltd. | Method of microvesicle enrichment |
| US20140004601A1 (en) * | 2010-12-20 | 2014-01-02 | Agency For Science, Technology And Research | Method of purifying exosomes |
| BR112015016136A2 (pt) * | 2013-01-03 | 2017-07-11 | Exosome Diagnostics Inc | métodos para isolar microvesículas |
| US10619152B2 (en) | 2014-02-10 | 2020-04-14 | Nancy Quan | Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents |
| US11268085B2 (en) * | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| SG11201700146QA (en) | 2014-07-09 | 2017-02-27 | Exosome Diagnostics Inc | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| CN104152436B (zh) * | 2014-08-01 | 2017-12-15 | 杭州新景生物试剂开发有限公司 | Dna分离纯化方法及其试剂盒 |
| CN107405540A (zh) * | 2014-10-24 | 2017-11-28 | 雅培分子公司 | 小核酸的富集 |
| CN105112400A (zh) * | 2015-08-31 | 2015-12-02 | 臻和（北京）科技有限公司 | 一种提取游离dna的试剂盒 |
| JP7037504B2 (ja) | 2016-05-13 | 2022-03-16 | エクソサム ダイアグノスティクス，インコーポレイティド | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
-
2017
- 2017-05-15 JP JP2018559313A patent/JP7037504B2/ja active Active
- 2017-05-15 EP EP22167997.0A patent/EP4079853A1/en active Pending
- 2017-05-15 WO PCT/US2017/032719 patent/WO2017197399A1/en not_active Ceased
- 2017-05-15 US US16/301,176 patent/US10808240B2/en active Active
- 2017-05-15 EP EP17728304.1A patent/EP3455355B1/en active Active
- 2017-05-15 AU AU2017263886A patent/AU2017263886B2/en active Active
- 2017-05-15 CN CN201780043334.9A patent/CN109642229A/zh active Pending
-
2020
- 2020-10-19 US US17/074,040 patent/US20210238582A1/en active Pending
-
2022
- 2022-03-04 JP JP2022033452A patent/JP7354327B2/ja active Active
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