JP2019521322A5 - - Google Patents
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- JP2019521322A5 JP2019521322A5 JP2018559313A JP2018559313A JP2019521322A5 JP 2019521322 A5 JP2019521322 A5 JP 2019521322A5 JP 2018559313 A JP2018559313 A JP 2018559313A JP 2018559313 A JP2018559313 A JP 2018559313A JP 2019521322 A5 JP2019521322 A5 JP 2019521322A5
- Authority
- JP
- Japan
- Prior art keywords
- bead
- buffer
- dna
- biological sample
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022033452A JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662336203P | 2016-05-13 | 2016-05-13 | |
| US62/336,203 | 2016-05-13 | ||
| PCT/US2017/032719 WO2017197399A1 (en) | 2016-05-13 | 2017-05-15 | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022033452A Division JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019521322A JP2019521322A (ja) | 2019-07-25 |
| JP2019521322A5 true JP2019521322A5 (enExample) | 2020-07-02 |
| JP7037504B2 JP7037504B2 (ja) | 2022-03-16 |
Family
ID=59014730
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018559313A Active JP7037504B2 (ja) | 2016-05-13 | 2017-05-15 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
| JP2022033452A Active JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022033452A Active JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US10808240B2 (enExample) |
| EP (2) | EP3455355B1 (enExample) |
| JP (2) | JP7037504B2 (enExample) |
| CN (1) | CN109642229A (enExample) |
| AU (1) | AU2017263886B2 (enExample) |
| WO (1) | WO2017197399A1 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11268085B2 (en) * | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| JP7037504B2 (ja) * | 2016-05-13 | 2022-03-16 | エクソサム ダイアグノスティクス，インコーポレイティド | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
| AU2017381410A1 (en) | 2016-12-23 | 2019-08-08 | Exopharm Limited | Methods and compositions for purification or isolation of microvesicles and exosomes |
| KR101875594B1 (ko) * | 2017-01-13 | 2018-07-06 | ㈜로제타엑소좀 | 금속을 이용한 세포밖 소포체의 분리 방법 |
| JP7225121B2 (ja) | 2017-05-17 | 2023-02-20 | エクソサム ダイアグノスティクス，インコーポレイティド | 微小胞核酸および／またはタンパク質、並びに腎移植拒絶反応マーカーとしてのその使用 |
| CN111148828A (zh) * | 2017-07-26 | 2020-05-12 | 罗塞塔外排体株式会社 | 利用阳离子分离细胞外囊泡的方法 |
| KR20210015740A (ko) * | 2017-09-20 | 2021-02-10 | 몰레큘러 스테서스코프, 인크. | 조직 상태를 검출하기 위한 방법 및 시스템 |
| MX2020014024A (es) | 2018-06-18 | 2021-03-09 | Exopharm Ltd | Metodos y composiciones para la purificacion o aislamiento de microvesiculas y exosomas. |
| US11119013B2 (en) * | 2018-10-12 | 2021-09-14 | Simpore Inc. | Extracellular vesicle isolation by nanomembranes |
| EP3884045B1 (en) | 2018-11-20 | 2023-01-11 | Exosome Diagnostics, Inc. | Methods for internal controls of microvesicle isolations |
| CN111254138A (zh) * | 2018-12-03 | 2020-06-09 | 北京银丰鼎诚生物工程技术有限公司 | 一种分子克隆中提高酶切产物浓缩效率的方法 |
| US12233356B2 (en) | 2019-03-21 | 2025-02-25 | Lonza Sales Ag | Process for preparing extracellular vesicles |
| GB201906722D0 (en) * | 2019-05-13 | 2019-06-26 | Ttp Plc | A method of preparing a sample for a diagnostic assay |
| EP4031865A2 (en) | 2019-09-18 | 2022-07-27 | Exosome Diagnostics, Inc. | Compositions, methods, and kits for the isolation of extracellular vesicles |
| EP4077660A1 (en) * | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Method for enriching vesicular rna |
| US20230028205A1 (en) * | 2019-12-16 | 2023-01-26 | Qiagen Gmbh | Enrichment method |
| JP2023508411A (ja) * | 2019-12-23 | 2023-03-02 | バイオプセンス オサケユキチュア | 血液試料からセルフリー核酸を抽出するための方法、自動化システムおよびカートリッジ |
| CN115461445A (zh) * | 2020-04-15 | 2022-12-09 | 合同会社予幸集团中央研究所 | 细胞外囊泡的回收方法 |
| WO2021217173A1 (en) * | 2020-04-21 | 2021-10-28 | Siemens Healthcare Diagnostics Inc. | Clinical specimen collection swabs for molecular diagnostic applications and methods of production and use thereof |
| WO2021243206A1 (en) | 2020-05-29 | 2021-12-02 | Exosome Diagnostics, Inc. | Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection |
| EP4217087A2 (en) * | 2020-09-23 | 2023-08-02 | Codiak BioSciences, Inc. | Process for preparing extracellular vesicles |
| CZ35067U1 (cs) * | 2020-12-15 | 2021-05-18 | Univerzita Palackého v Olomouci | Sada pro izolaci RNA |
| JP7773765B2 (ja) * | 2020-12-18 | 2025-11-20 | 国立研究開発法人産業技術総合研究所 | 回収用キットおよび核酸含有溶液の調製方法 |
| US20220315992A1 (en) * | 2021-04-06 | 2022-10-06 | ID Match, LLC | Method for identification of urine drug testing sample |
| US20240287498A1 (en) * | 2021-06-17 | 2024-08-29 | Qiagen Gmbh | Method for isolating non-vesicular mirna |
| US20250075258A1 (en) | 2021-07-16 | 2025-03-06 | Exosome Diagnostics, Inc. | Methods of detecting sjogren's syndrome using salivary exosomes |
| DE102021208893B3 (de) | 2021-08-13 | 2022-11-17 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Isolieren von Analyten unterschiedlicher Analyt-Klassen |
| SE2100130A1 (en) * | 2021-09-01 | 2023-03-02 | Frontline Diagnostics AB | System for integrated automated isolation and analysis of biological vesicles and associated molecules |
| CA3236331A1 (en) * | 2021-11-04 | 2023-05-11 | Mayo Foundation For Medical Education And Research | Methods and materials for treating cancer |
| EP4479561A1 (en) | 2022-02-18 | 2024-12-25 | Exosome Diagnostics, Inc. | Use of microvesicle signatures in the identification and treatment of renal disorders |
| US20260078448A1 (en) | 2022-09-07 | 2026-03-19 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| WO2024185655A1 (ja) * | 2023-03-06 | 2024-09-12 | 東ソー株式会社 | 細胞外小胞精製用磁性粒子およびその製造方法、ならびに、細胞外小胞の精製方法 |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| AU645915B2 (en) | 1991-07-23 | 1994-01-27 | F. Hoffmann-La Roche Ag | Improvements in the in situ PCR |
| US5605798A (en) | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
| US5639606A (en) | 1993-04-06 | 1997-06-17 | The University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| US5556773A (en) | 1993-08-06 | 1996-09-17 | Yourno; Joseph | Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes |
| CA2270132A1 (en) | 1996-11-06 | 1998-05-14 | Sequenom, Inc. | Dna diagnostics based on mass spectrometry |
| US6994960B1 (en) | 1997-05-28 | 2006-02-07 | The Walter And Eliza Hall Institute Of Medical Research | Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage |
| US6004755A (en) | 1998-04-07 | 1999-12-21 | Incyte Pharmaceuticals, Inc. | Quantitative microarray hybridizaton assays |
| FR2788780B1 (fr) | 1999-01-27 | 2001-03-30 | Ap Cells Inc | Procede de preparation de vesicules membranaires |
| GB9927320D0 (en) | 1999-11-18 | 2000-01-12 | Chiron Spa | Exosome separation |
| US6812023B1 (en) | 2000-04-27 | 2004-11-02 | Anosys, Inc. | Methods of producing membrane vesicles |
| US6913879B1 (en) | 2000-07-10 | 2005-07-05 | Telechem International Inc. | Microarray method of genotyping multiple samples at multiple LOCI |
| US6696271B2 (en) | 2001-08-23 | 2004-02-24 | The Regents Of The University Of California | Frozen tissue microarray technology for analysis of RNA, DNA, and proteins |
| WO2003023065A1 (en) | 2001-09-06 | 2003-03-20 | Syngenta Participations Ag | Dna methylation patterns |
| EP2339025B1 (en) | 2002-06-26 | 2013-10-09 | Cold Spring Harbor Laboratory | Methods for determining the methylation profiles |
| US7364848B2 (en) | 2002-09-02 | 2008-04-29 | Pamgene B.V. | Integrated microarray analysis |
| US7141371B2 (en) | 2002-09-06 | 2006-11-28 | State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Methods for detecting and localizing DNA mutations by microarray |
| JP2008510456A (ja) * | 2004-07-30 | 2008-04-10 | アジェンコート バイオサイエンス コーポレーション | 多官能基コート化固相担体を用いる核酸の単離方法 |
| EP1869221A2 (en) | 2005-04-15 | 2007-12-26 | Cedars-Sinai Medical Center | Flow-cytometric heteroduplex analysis for detection of genetic alterations |
| US20060286557A1 (en) * | 2005-06-15 | 2006-12-21 | Basehore Lee S | Combined lysis and PCR buffer |
| US9428746B2 (en) * | 2007-10-31 | 2016-08-30 | Akonni Biosystems, Inc. | Method and kit for purifying nucleic acids |
| CN105734128B (zh) | 2008-02-01 | 2021-05-18 | 总医院有限公司 | 微泡在医学疾病和病况的诊断、预后以及治疗中的用途 |
| DK2454588T3 (en) * | 2009-07-16 | 2015-09-28 | Gen Hospital Corp | Nucleic acid |
| WO2012054975A1 (en) * | 2010-10-28 | 2012-05-03 | Clinical Genomics Pty. Ltd. | Method of microvesicle enrichment |
| WO2012087241A1 (en) * | 2010-12-20 | 2012-06-28 | Agency For Science, Technology And Research | Method of purifying exosomes |
| CA2897207C (en) * | 2013-01-03 | 2022-08-02 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles |
| WO2015120445A1 (en) * | 2014-02-10 | 2015-08-13 | Nancy Quan | Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents |
| US11268085B2 (en) * | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| CA2954576C (en) * | 2014-07-09 | 2020-08-11 | Daniel ENDERLE | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| CN104152436B (zh) * | 2014-08-01 | 2017-12-15 | 杭州新景生物试剂开发有限公司 | Dna分离纯化方法及其试剂盒 |
| US9783799B2 (en) * | 2014-10-24 | 2017-10-10 | Abbott Molecular Inc. | Enrichment of small nucleic acids |
| CN105112400A (zh) * | 2015-08-31 | 2015-12-02 | 臻和（北京）科技有限公司 | 一种提取游离dna的试剂盒 |
| JP7037504B2 (ja) * | 2016-05-13 | 2022-03-16 | エクソサム ダイアグノスティクス，インコーポレイティド | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
-
2017
- 2017-05-15 JP JP2018559313A patent/JP7037504B2/ja active Active
- 2017-05-15 CN CN201780043334.9A patent/CN109642229A/zh active Pending
- 2017-05-15 US US16/301,176 patent/US10808240B2/en active Active
- 2017-05-15 WO PCT/US2017/032719 patent/WO2017197399A1/en not_active Ceased
- 2017-05-15 EP EP17728304.1A patent/EP3455355B1/en active Active
- 2017-05-15 AU AU2017263886A patent/AU2017263886B2/en active Active
- 2017-05-15 EP EP22167997.0A patent/EP4079853A1/en active Pending
-
2020
- 2020-10-19 US US17/074,040 patent/US20210238582A1/en active Pending
-
2022
- 2022-03-04 JP JP2022033452A patent/JP7354327B2/ja active Active
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