JP7037504B2 - 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 - Google Patents

生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 Download PDF

Info

Publication number
JP7037504B2
JP7037504B2 JP2018559313A JP2018559313A JP7037504B2 JP 7037504 B2 JP7037504 B2 JP 7037504B2 JP 2018559313 A JP2018559313 A JP 2018559313A JP 2018559313 A JP2018559313 A JP 2018559313A JP 7037504 B2 JP7037504 B2 JP 7037504B2
Authority
JP
Japan
Prior art keywords
rna
dna
nucleic acid
sample
membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018559313A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019521322A (ja
JP2019521322A5 (enExample
Inventor
シュトール ゲオルク
エンダーレ ダニエル
ノアーホルム ミッケル
カール オラブ スコグ ジョアン
Original Assignee
エクソサム ダイアグノスティクス，インコーポレイティド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by エクソサム ダイアグノスティクス，インコーポレイティド filed Critical エクソサム ダイアグノスティクス，インコーポレイティド
Publication of JP2019521322A publication Critical patent/JP2019521322A/ja
Publication of JP2019521322A5 publication Critical patent/JP2019521322A5/ja
Priority to JP2022033452A priority Critical patent/JP7354327B2/ja
Application granted granted Critical
Publication of JP7037504B2 publication Critical patent/JP7037504B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2018559313A 2016-05-13 2017-05-15 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 Active JP7037504B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022033452A JP7354327B2 (ja) 2016-05-13 2022-03-04 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662336203P 2016-05-13 2016-05-13
US62/336,203 2016-05-13
PCT/US2017/032719 WO2017197399A1 (en) 2016-05-13 2017-05-15 Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022033452A Division JP7354327B2 (ja) 2016-05-13 2022-03-04 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法

Publications (3)

Publication Number Publication Date
JP2019521322A JP2019521322A (ja) 2019-07-25
JP2019521322A5 JP2019521322A5 (enExample) 2020-07-02
JP7037504B2 true JP7037504B2 (ja) 2022-03-16

Family

ID=59014730

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2018559313A Active JP7037504B2 (ja) 2016-05-13 2017-05-15 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法
JP2022033452A Active JP7354327B2 (ja) 2016-05-13 2022-03-04 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2022033452A Active JP7354327B2 (ja) 2016-05-13 2022-03-04 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法

Country Status (7)

Country Link
US (2) US10808240B2 (enExample)
EP (2) EP4079853A1 (enExample)
JP (2) JP7037504B2 (enExample)
CN (1) CN109642229A (enExample)
AU (1) AU2017263886B2 (enExample)
CA (1) CA3022528A1 (enExample)
WO (1) WO2017197399A1 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11268085B2 (en) * 2014-05-27 2022-03-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
US10808240B2 (en) 2016-05-13 2020-10-20 Exosome Diagnostics, Inc. Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids
KR20190100174A (ko) 2016-12-23 2019-08-28 엑소팜 리미티드 미세소포 및 엑소좀의 정제 또는 단리를 위한 방법 및 조성물
KR101875594B1 (ko) * 2017-01-13 2018-07-06 ㈜로제타엑소좀 금속을 이용한 세포밖 소포체의 분리 방법
EP3635139A1 (en) 2017-05-17 2020-04-15 Exosome Diagnostics, Inc. Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection
CN111148828A (zh) * 2017-07-26 2020-05-12 罗塞塔外排体株式会社 利用阳离子分离细胞外囊泡的方法
EP3807616A4 (en) * 2018-06-18 2022-03-23 Exopharm Limited METHODS AND COMPOSITIONS FOR PURIFICATION OR ISOLATION OF MICROVESICLES AND EXOSOMES
WO2020077294A1 (en) * 2018-10-12 2020-04-16 Simpore, Inc. Extracellular vesicle isolation by nanomembranes
WO2020106853A1 (en) 2018-11-20 2020-05-28 Exosome Diagnostics, Inc. Compositions and methods for internal controls of microvesicle isolations
CN111254138A (zh) * 2018-12-03 2020-06-09 北京银丰鼎诚生物工程技术有限公司 一种分子克隆中提高酶切产物浓缩效率的方法
EP3941607A1 (en) 2019-03-21 2022-01-26 Codiak BioSciences, Inc. Process for preparing extracellular vesicles
GB201906722D0 (en) * 2019-05-13 2019-06-26 Ttp Plc A method of preparing a sample for a diagnostic assay
CN110106169A (zh) * 2019-05-28 2019-08-09 浙江大学 一种提高无细胞体液中微量核酸捕获率的方法
EP4031865A2 (en) 2019-09-18 2022-07-27 Exosome Diagnostics, Inc. Compositions, methods, and kits for the isolation of extracellular vesicles
EP4077659A1 (en) 2019-12-16 2022-10-26 QIAGEN GmbH Enrichment method
EP4077660A1 (en) 2019-12-16 2022-10-26 QIAGEN GmbH Method for enriching vesicular rna
JP2023508411A (ja) * 2019-12-23 2023-03-02 バイオプセンス オサケユキチュア 血液試料からセルフリー核酸を抽出するための方法、自動化システムおよびカートリッジ
CN115461445A (zh) * 2020-04-15 2022-12-09 合同会社予幸集团中央研究所 细胞外囊泡的回收方法
WO2021217173A1 (en) * 2020-04-21 2021-10-28 Siemens Healthcare Diagnostics Inc. Clinical specimen collection swabs for molecular diagnostic applications and methods of production and use thereof
US20230203587A1 (en) 2020-05-29 2023-06-29 Exosome Diagnostics, Inc. Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
WO2022066934A2 (en) * 2020-09-23 2022-03-31 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
CZ35067U1 (cs) * 2020-12-15 2021-05-18 Univerzita Palackého v Olomouci Sada pro izolaci RNA
JP7773765B2 (ja) * 2020-12-18 2025-11-20 国立研究開発法人産業技術総合研究所 回収用キットおよび核酸含有溶液の調製方法
US20220315992A1 (en) * 2021-04-06 2022-10-06 ID Match, LLC Method for identification of urine drug testing sample
WO2022263500A1 (en) * 2021-06-17 2022-12-22 Qiagen Gmbh Method for isolating non-vesicular mirna
US20250075258A1 (en) 2021-07-16 2025-03-06 Exosome Diagnostics, Inc. Methods of detecting sjogren's syndrome using salivary exosomes
DE102021208893B3 (de) 2021-08-13 2022-11-17 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Isolieren von Analyten unterschiedlicher Analyt-Klassen
SE2100130A1 (en) * 2021-09-01 2023-03-02 Frontline Diagnostics AB System for integrated automated isolation and analysis of biological vesicles and associated molecules
US20250009797A1 (en) * 2021-11-04 2025-01-09 Mayo Foundation For Medical Education And Research Methods and materials for treating cancer
AU2023221644A1 (en) 2022-02-18 2024-09-12 Bio-Techne Corporation Use of microvesicle signatures in the identification and treatment of renal disorders
WO2024054572A1 (en) 2022-09-07 2024-03-14 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
WO2024185655A1 (ja) * 2023-03-06 2024-09-12 東ソー株式会社 細胞外小胞精製用磁性粒子およびその製造方法、ならびに、細胞外小胞の精製方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008510456A (ja) 2004-07-30 2008-04-10 アジェンコート バイオサイエンス コーポレーション 多官能基コート化固相担体を用いる核酸の単離方法
US20150010951A1 (en) 2010-10-28 2015-01-08 Clinical Genomics Pty. Ltd. Method of microvesicle enrichment
WO2016007755A1 (en) 2014-07-09 2016-01-14 Skog Johan Karl Olov Methods for isolating microvesicles and extracting nucleic acids from biological samples
JP2016502862A (ja) 2013-01-03 2016-02-01 エクソサム ダイアグノスティクス，インコーポレイティド 微細小胞の単離方法
US20160115471A1 (en) 2014-10-24 2016-04-28 Dae Hyun H. Kim Enrichment of small nucleic acids

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
AU645915B2 (en) 1991-07-23 1994-01-27 F. Hoffmann-La Roche Ag Improvements in the in situ PCR
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5556773A (en) 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
CA2270132A1 (en) 1996-11-06 1998-05-14 Sequenom, Inc. Dna diagnostics based on mass spectrometry
EP0985148A4 (en) 1997-05-28 2004-03-10 Inst Medical W & E Hall DIAGNOSIS OF NUCLEIC ACIDS BY MASS SPECTROMETRY, MASS SEPARATION AND BASE-SPECIFIC CLEAVING
US6004755A (en) 1998-04-07 1999-12-21 Incyte Pharmaceuticals, Inc. Quantitative microarray hybridizaton assays
FR2788780B1 (fr) 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
US6913879B1 (en) 2000-07-10 2005-07-05 Telechem International Inc. Microarray method of genotyping multiple samples at multiple LOCI
US6696271B2 (en) 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins
WO2003023065A1 (en) 2001-09-06 2003-03-20 Syngenta Participations Ag Dna methylation patterns
ES2431944T3 (es) 2002-06-26 2013-11-28 Cold Spring Harbor Laboratory Métodos para determinar los perfiles de metilación
AU2003267065A1 (en) 2002-09-02 2004-03-19 Pamgene B.V. Novel integrated microarray analysis
US7141371B2 (en) 2002-09-06 2006-11-28 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods for detecting and localizing DNA mutations by microarray
EP1869221A2 (en) 2005-04-15 2007-12-26 Cedars-Sinai Medical Center Flow-cytometric heteroduplex analysis for detection of genetic alterations
US20060286557A1 (en) * 2005-06-15 2006-12-21 Basehore Lee S Combined lysis and PCR buffer
US9428746B2 (en) * 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
WO2011009104A1 (en) * 2009-07-16 2011-01-20 The General Hospital Corporation Nucleic acid analysis
EP2245199B1 (en) 2008-02-01 2013-11-13 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
SG190450A1 (en) * 2010-12-20 2013-06-28 Agency Science Tech & Res Method of purifying exosomes
US10619152B2 (en) * 2014-02-10 2020-04-14 Nancy Quan Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents
US11268085B2 (en) * 2014-05-27 2022-03-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
CN104152436B (zh) * 2014-08-01 2017-12-15 杭州新景生物试剂开发有限公司 Dna分离纯化方法及其试剂盒
CN105112400A (zh) * 2015-08-31 2015-12-02 臻和（北京）科技有限公司 一种提取游离dna的试剂盒
US10808240B2 (en) * 2016-05-13 2020-10-20 Exosome Diagnostics, Inc. Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008510456A (ja) 2004-07-30 2008-04-10 アジェンコート バイオサイエンス コーポレーション 多官能基コート化固相担体を用いる核酸の単離方法
US20150010951A1 (en) 2010-10-28 2015-01-08 Clinical Genomics Pty. Ltd. Method of microvesicle enrichment
JP2016502862A (ja) 2013-01-03 2016-02-01 エクソサム ダイアグノスティクス，インコーポレイティド 微細小胞の単離方法
WO2016007755A1 (en) 2014-07-09 2016-01-14 Skog Johan Karl Olov Methods for isolating microvesicles and extracting nucleic acids from biological samples
US20160115471A1 (en) 2014-10-24 2016-04-28 Dae Hyun H. Kim Enrichment of small nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ENDERLE D; ET AL，DEVELOPMENT OF A ONE-STEP ISOLATION PLATFORM FOR EXOSOMAL RNA AND CIRCULATING CELL-FREE DNA FROM CANCER PLASMA SAMPLES，EUROPEAN JOURNAL OF CANCER - POSTER SESSION，2014年11月，VOL:50, NR:SUPPL.6，PAGE(S):102，http://dx.doi.org/10.1016/S0959-8049(14)70439-3

Also Published As

Publication number Publication date
US20210238582A1 (en) 2021-08-05
JP2022084700A (ja) 2022-06-07
EP4079853A1 (en) 2022-10-26
EP3455355A1 (en) 2019-03-20
US20190284548A1 (en) 2019-09-19
US10808240B2 (en) 2020-10-20
AU2017263886A1 (en) 2018-11-22
JP2019521322A (ja) 2019-07-25
JP7354327B2 (ja) 2023-10-02
CA3022528A1 (en) 2017-11-16
AU2017263886B2 (en) 2023-11-09
EP3455355B1 (en) 2022-04-13
WO2017197399A1 (en) 2017-11-16
CN109642229A (zh) 2019-04-16

Similar Documents

Publication Publication Date Title
JP7037504B2 (ja) 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法
JP7128866B2 (ja) 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法
JP6449781B2 (ja) 微細小胞の単離方法
JP2022115972A (ja) エキソソーム結合型核酸の配列決定および分析
EP3452613B1 (en) Profiling microvesicle nucleic acids and uses thereof as signatures in diagnosis of renal transplant rejection
US11268085B2 (en) Methods for isolating microvesicles and extracting nucleic acids from biological samples
HK1238676A1 (en) Methods for isolating microvesicles and extracting nucleic acids from biological samples

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200515

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20200515

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210406

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210706

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211006

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20220104

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20220203

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20220304

R150 Certificate of patent or registration of utility model

Ref document number: 7037504

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250