AU2017263886B2 - Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids - Google Patents

Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids Download PDF

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Publication number
AU2017263886B2
AU2017263886B2 AU2017263886A AU2017263886A AU2017263886B2 AU 2017263886 B2 AU2017263886 B2 AU 2017263886B2 AU 2017263886 A AU2017263886 A AU 2017263886A AU 2017263886 A AU2017263886 A AU 2017263886A AU 2017263886 B2 AU2017263886 B2 AU 2017263886B2
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microvesicles
dna
rna
capture surface
bead
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AU2017263886A1 (en
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Daniel ENDERLE
Mikkel Noerholm
Johan Karl Olov Skog
Georg Stoll
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Exosome Diagnostics Inc
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Exosome Diagnostics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2017263886A 2016-05-13 2017-05-15 Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids Active AU2017263886B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662336203P 2016-05-13 2016-05-13
US62/336,203 2016-05-13
PCT/US2017/032719 WO2017197399A1 (en) 2016-05-13 2017-05-15 Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids

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AU2017263886A1 AU2017263886A1 (en) 2018-11-22
AU2017263886B2 true AU2017263886B2 (en) 2023-11-09

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US (2) US10808240B2 (enExample)
EP (2) EP3455355B1 (enExample)
JP (2) JP7037504B2 (enExample)
CN (1) CN109642229A (enExample)
AU (1) AU2017263886B2 (enExample)
WO (1) WO2017197399A1 (enExample)

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US11904259B2 (en) * 2017-07-26 2024-02-20 Rosetta Exosome Method for isolating extracellular vesicles using cations
CN112585446A (zh) * 2018-06-18 2021-03-30 埃克波菲尔有限公司 用于纯化或分离微泡和外泌体的方法与组合物
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WO2021243206A1 (en) 2020-05-29 2021-12-02 Exosome Diagnostics, Inc. Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
CA3193107A1 (en) * 2020-09-23 2022-03-31 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
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JP7773765B2 (ja) * 2020-12-18 2025-11-20 国立研究開発法人産業技術総合研究所 回収用キットおよび核酸含有溶液の調製方法
US20220315992A1 (en) * 2021-04-06 2022-10-06 ID Match, LLC Method for identification of urine drug testing sample
EP4355873A1 (en) * 2021-06-17 2024-04-24 QIAGEN GmbH Method for isolating non-vesicular mirna
WO2023288130A1 (en) 2021-07-16 2023-01-19 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
DE102021208893B3 (de) 2021-08-13 2022-11-17 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Isolieren von Analyten unterschiedlicher Analyt-Klassen
SE2100130A1 (en) * 2021-09-01 2023-03-02 Frontline Diagnostics AB System for integrated automated isolation and analysis of biological vesicles and associated molecules
AU2022379949A1 (en) * 2021-11-04 2024-05-02 Mayo Foundation For Medical Education And Research Methods and materials for treating cancer
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EP4584397A1 (en) 2022-09-07 2025-07-16 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
WO2024185655A1 (ja) * 2023-03-06 2024-09-12 東ソー株式会社 細胞外小胞精製用磁性粒子およびその製造方法、ならびに、細胞外小胞の精製方法

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Publication number Publication date
US20190284548A1 (en) 2019-09-19
WO2017197399A1 (en) 2017-11-16
AU2017263886A1 (en) 2018-11-22
EP4079853A1 (en) 2022-10-26
US10808240B2 (en) 2020-10-20
JP7354327B2 (ja) 2023-10-02
JP2022084700A (ja) 2022-06-07
CA3022528A1 (en) 2017-11-16
JP7037504B2 (ja) 2022-03-16
US20210238582A1 (en) 2021-08-05
EP3455355A1 (en) 2019-03-20
EP3455355B1 (en) 2022-04-13
JP2019521322A (ja) 2019-07-25
CN109642229A (zh) 2019-04-16

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