BR112015016136A2 - métodos para isolar microvesículas - Google Patents

métodos para isolar microvesículas

Info

Publication number
BR112015016136A2
BR112015016136A2 BR112015016136A BR112015016136A BR112015016136A2 BR 112015016136 A2 BR112015016136 A2 BR 112015016136A2 BR 112015016136 A BR112015016136 A BR 112015016136A BR 112015016136 A BR112015016136 A BR 112015016136A BR 112015016136 A2 BR112015016136 A2 BR 112015016136A2
Authority
BR
Brazil
Prior art keywords
methods
microvesicles
isolate microvesicles
isolate
isolating
Prior art date
Application number
BR112015016136A
Other languages
English (en)
Inventor
Ramachandran Aparna
Enderle Daniel
Berghoff Emily
Yan Haoheng
Karl Olov Skog Johan
Noerholm Mikkel
Wei Tai-Fen
Original Assignee
Exosome Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exosome Diagnostics Inc filed Critical Exosome Diagnostics Inc
Publication of BR112015016136A2 publication Critical patent/BR112015016136A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

resumo “métodos para isolar microvesículas” a invenção fornece novos métodos para isolar microvesículas de uma amostra biológica e para extrair ácidos nucleicos das microvesículas.
BR112015016136A 2013-01-03 2014-01-03 métodos para isolar microvesículas BR112015016136A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361748575P 2013-01-03 2013-01-03
PCT/US2014/010173 WO2014107571A1 (en) 2013-01-03 2014-01-03 Methods for isolating microvesicles

Publications (1)

Publication Number Publication Date
BR112015016136A2 true BR112015016136A2 (pt) 2017-07-11

Family

ID=51062472

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015016136A BR112015016136A2 (pt) 2013-01-03 2014-01-03 métodos para isolar microvesículas

Country Status (11)

Country Link
US (2) US20150353920A1 (pt)
EP (2) EP2941629B1 (pt)
JP (1) JP6449781B2 (pt)
CN (2) CN105026911B (pt)
AU (2) AU2014203987B2 (pt)
BR (1) BR112015016136A2 (pt)
CA (1) CA2897207C (pt)
HK (1) HK1217365A1 (pt)
IL (2) IL239786B (pt)
MX (2) MX366153B (pt)
WO (1) WO2014107571A1 (pt)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3039234C (en) * 2011-05-11 2023-03-28 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
ES2708561T3 (es) 2013-03-14 2019-04-10 Translate Bio Inc Métodos para la purificación de ARN mensajero
US20170165334A1 (en) * 2015-12-11 2017-06-15 Tianxin Wang Methods to Treat Diseases with Protein, Peptide, Antigen Modification and Hemopurification
US11268085B2 (en) 2014-05-27 2022-03-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
US10465183B2 (en) 2014-07-09 2019-11-05 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
WO2016007951A1 (en) * 2014-07-11 2016-01-14 KARNAKIS, Jennifer A. Compositions and methods for detecting rare sequence variants in nucleic acid sequencing
CA2965500A1 (en) * 2014-10-24 2016-04-28 Abbott Molecular Inc. Enrichment of small nucleic acids
EP3112474A1 (en) 2015-06-29 2017-01-04 Evonik Degussa GmbH Method of detecting avian necrotic enteritis
WO2017062901A2 (en) * 2015-10-07 2017-04-13 Tymora Analytical Operations Llc Methods to isolate extracellular vesicles in biofluids for biomarker discovery and disease diagnosis
CN109563547A (zh) 2016-04-15 2019-04-02 外来体诊断公司 间变性淋巴瘤激酶(alk)核酸和alk融合转录物的基于血浆的检测及其在癌症诊断和治疗中的用途
CN105716923A (zh) * 2016-04-21 2016-06-29 华南理工大学 一种聚合物囊泡扫描电镜样品的制备方法
US11214833B2 (en) 2016-05-05 2022-01-04 Exosome Diagnostics, Inc. Profiling microvesicle nucleic acids and uses thereof as signatures in diagnosis of renal transplant rejection
EP4079853A1 (en) 2016-05-13 2022-10-26 Exosome Diagnostics, Inc. Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids
CN109891236A (zh) * 2016-10-13 2019-06-14 合同会社明乐康中央研究所 回收胞外囊泡的方法
US11396676B2 (en) 2016-10-21 2022-07-26 Exosome Diagnostics, Inc. Sequencing and analysis of exosome associated nucleic acids
AU2017366813B2 (en) 2016-11-30 2023-04-20 Exosome Diagnostics, Inc. Methods and compositions to detect mutations in plasma using exosomal RNA and cell free DNA from non-small cell lung cancer patients
CA3043776A1 (en) * 2016-12-23 2018-06-28 Chacko JOSEPH Methods and compositions for purification or isolation of microvesicles and exosomes
EP3562959A2 (en) 2017-01-02 2019-11-06 Exosome Diagnostics, Inc. Methods to distinguish rna and dna in a combined preparation
KR101875594B1 (ko) 2017-01-13 2018-07-06 ㈜로제타엑소좀 금속을 이용한 세포밖 소포체의 분리 방법
ES2944359T3 (es) 2017-05-12 2023-06-20 Evonik Operations Gmbh Procedimiento de detección de enfermedades inducidas por C. perfringens en una bandada de aves
EP3635139A1 (en) 2017-05-17 2020-04-15 Exosome Diagnostics, Inc. Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection
CN107271655A (zh) * 2017-05-18 2017-10-20 成都中医药大学附属医院 一种检测尿外泌体荷载miRNAs的试剂盒和方法
EP3652315A4 (en) * 2017-07-12 2021-09-01 Exosome Diagnostics, Inc. METHOD OF ISOLATION AND ENRICHMENT OF POPULATIONS OF EXTRACELLULAR VESICULES FROM A BIOFLUID AND METHOD OF USING THEREOF
US11390864B2 (en) * 2017-07-12 2022-07-19 Illumina, Inc. Nucleic acid extraction materials, systems, and methods
EP3655531A4 (en) * 2017-07-18 2021-09-08 Exosome Diagnostics, Inc. SEQUENCING OF NUCLEIC ACIDS ASSOCIATED WITH EXOSOMAL ISOLATION IN PATIENTS WITH MULTIFORM GLIOBLASTOMA
US11904259B2 (en) * 2017-07-26 2024-02-20 Rosetta Exosome Method for isolating extracellular vesicles using cations
US11260347B2 (en) * 2017-12-26 2022-03-01 Limited Liability Company “Prostagnost” Method and device for separating extracellular vesicles from biological liquids with the aid of cascade ultrafiltration
CN112739832A (zh) 2018-06-06 2021-04-30 外来体诊断公司 用于开发尿液生物标志物和用于检测膀胱癌的方法
CN108841777A (zh) * 2018-06-22 2018-11-20 北京恩泽康泰生物科技有限公司 基于静电吸附的胞外囊泡及其内含物的提取方法及装置
AU2019292939A1 (en) * 2018-06-27 2021-01-07 The Trustees Of Indiana University Methods for analyzing DNA in urine
CN109337859A (zh) * 2018-10-08 2019-02-15 南昌大学 一种分离细胞表面囊泡的方法
EP3875570A4 (en) * 2018-10-30 2022-08-24 Craif Inc. DEVICE FOR USE IN CAPTURING EXTRACELLULAR VESICLES, AND METHOD FOR STORAGE AND METHOD FOR TRANSPORTING EXTRACELLULAR VESICLES
US20210403881A1 (en) 2018-11-20 2021-12-30 Exosome Diagnostics, Inc. Compositions and methods for internal controls of microvesicle isolations
CN113748217B (zh) 2019-03-01 2023-08-25 仁慈生物分析公司 用于靶实体检测的系统、组合物和方法
US20220387906A1 (en) * 2019-03-21 2022-12-08 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
CN112048462B (zh) * 2019-06-05 2022-08-23 北京丰特云基科技发展有限公司 一种基于阴离子多聚物修饰基质的细胞外囊泡分离富集方法
EP4031865A2 (en) 2019-09-18 2022-07-27 Exosome Diagnostics, Inc. Compositions, methods, and kits for the isolation of extracellular vesicles
WO2021122855A1 (en) 2019-12-16 2021-06-24 Qiagen Gmbh Method for enriching vesicular rna
EP4077659A1 (en) 2019-12-16 2022-10-26 QIAGEN GmbH Enrichment method
JP7425403B2 (ja) 2020-02-17 2024-01-31 株式会社Ｊｖｃケンウッド 生体試料分析方法
CN113495029A (zh) * 2020-04-01 2021-10-12 杨昆德 用于分离欲求物质的装置和方法
CN115698265A (zh) * 2020-04-24 2023-02-03 高丽大学校算学协力团 微囊泡分离方法及微囊泡分离装置
WO2021243206A1 (en) 2020-05-29 2021-12-02 Exosome Diagnostics, Inc. Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
WO2022066934A2 (en) * 2020-09-23 2022-03-31 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
CN112391382B (zh) * 2020-12-07 2022-10-11 湖北盛齐安生物科技股份有限公司 一种快速提取囊泡dna的方法
WO2023288130A1 (en) 2021-07-16 2023-01-19 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
WO2023158869A1 (en) 2022-02-18 2023-08-24 Exosome Diagnostics, Inc. Use of microvesicle signatures in the identification and treatment of renal disorders
WO2024054572A1 (en) 2022-09-07 2024-03-14 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
FR2788780B1 (fr) 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
JP2004105009A (ja) * 2002-09-13 2004-04-08 Dojindo Laboratories テトラフェニルホウ素化合物から成る核酸分離用試薬とそれを用いる核酸分離方法
US20110053157A1 (en) 2008-02-01 2011-03-03 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
WO2010065765A2 (en) * 2008-12-04 2010-06-10 Aethlon Medical, Inc. Affinity capture of circulating biomarkers
WO2010141862A2 (en) * 2009-06-05 2010-12-09 Mayo Foundation For Medical Education And Research Methods and materials for isolating exosomes
KR101314868B1 (ko) * 2009-07-01 2013-10-08 주식회사이언메딕스 포유류의 유핵세포에서 유래된 마이크로베시클 및 이의 용도
EP2591359B1 (en) * 2010-07-07 2017-03-01 Aethlon Medical Inc Methods for quantifying exosomes
JP6219721B2 (ja) * 2010-11-10 2017-10-25 エキソソーム ダイアグノスティックス インコーポレイテッド 核酸含有粒子の単離および該粒子からの核酸の抽出のための方法
SG190450A1 (en) * 2010-12-20 2013-06-28 Agency Science Tech & Res Method of purifying exosomes
CA2839530A1 (en) * 2011-06-16 2012-12-20 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Biomarker compositions and methods

Also Published As

Publication number Publication date
MX2015008727A (es) 2016-04-21
HK1217365A1 (zh) 2017-01-06
US20210171934A1 (en) 2021-06-10
EP2941629A1 (en) 2015-11-11
AU2014203987A1 (en) 2015-08-20
AU2018202789A1 (en) 2018-05-10
WO2014107571A1 (en) 2014-07-10
AU2018202789B2 (en) 2020-01-02
CN110106229A (zh) 2019-08-09
EP3499212A1 (en) 2019-06-19
IL239786A0 (en) 2015-08-31
IL239786B (en) 2019-07-31
CA2897207A1 (en) 2014-07-10
EP2941629A4 (en) 2017-03-22
CN110106229B (zh) 2023-03-28
IL267526A (en) 2019-08-29
JP6449781B2 (ja) 2019-01-09
JP2016502862A (ja) 2016-02-01
EP2941629B1 (en) 2018-10-10
CA2897207C (en) 2022-08-02
AU2014203987B2 (en) 2018-01-25
CN105026911B (zh) 2019-01-22
EP3499212B1 (en) 2020-08-12
MX2019007742A (es) 2019-08-29
US20150353920A1 (en) 2015-12-10
CN105026911A (zh) 2015-11-04
MX366153B (es) 2019-06-28
IL267526B (en) 2020-10-29

Similar Documents

Publication Publication Date Title
BR112015016136A2 (pt) métodos para isolar microvesículas
IL280307B (en) Methods for isolating microbubbles and extracting nucleic acids from biological samples
PT3708668T (pt) Moléculas de ácido nucleico artificiais para uma expressão melhorada de proteína
SG11201704681QA (en) Artificial nucleic acid molecules
DK3154995T3 (da) Separation of 2'-o-fucosyllactose from fermentation broth
EP3161162A4 (en) Analysis of nucleic acid sequences
BR112016010095A2 (pt) ácidos nucleicos de célula livre para a ánalise de microbioma humano e componentes do mesmos.
SG10202111841TA (en) Nanopore-based analysis of protein characteristics
BR112016015906A2 (pt) sistema de análise de produção de poço de óleo
IN2015DN02479A (pt)
EP3218698A4 (en) Biological sample collection and storage assembly
EP3240885A4 (en) STRUCTURED BIOLOGICAL SAMPLES FOR ANALYSIS BY A MASS CYTOMETER
EP3260035A4 (en) BIOLOGICAL OBSERVATION SYSTEM
SG10201500149XA (en) Method using fluorescent turn-on probes for cell-specific detection
PT3407798T (pt) Dispositivo de colheita de amostras biológicas
HK1247370A1 (zh) 組織樣本分析技術
IL248080A0 (en) System and method for identifying sources associated with biological arrhythmias
ZA201701382B (en) Lexical dialect analysis system
GB201402347D0 (en) Fixative solution for materials or samples of biological origin
DK3317422T3 (da) Diagnostisk sæt til viskoelasticitetsanalyse samt anvendelser deraf
ES1113930Y (es) Unidad biologica para caracoles
GB201403790D0 (en) Microfluidic devices and arrangements for introducing reagents and biological samples to such devices
ITUB20160397A1 (it) Contenitore per la raccolta di campioni di feci
EP3405095C0 (en) TISSUE SAMPLE ANALYSIS
SG10201407814VA (en) System and method for analyzing biological sample

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]