JP2018524978A5 - - Google Patents
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- JP2018524978A5 JP2018524978A5 JP2017564901A JP2017564901A JP2018524978A5 JP 2018524978 A5 JP2018524978 A5 JP 2018524978A5 JP 2017564901 A JP2017564901 A JP 2017564901A JP 2017564901 A JP2017564901 A JP 2017564901A JP 2018524978 A5 JP2018524978 A5 JP 2018524978A5
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- 239000000523 sample Substances 0.000 claims 22
- 239000003153 chemical reaction reagent Substances 0.000 claims 13
- 108091006146 Channels Proteins 0.000 claims 9
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 7
- 108090000790 Enzymes Proteins 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 4
- 230000011987 methylation Effects 0.000 claims 4
- 238000007069 methylation reaction Methods 0.000 claims 4
- 238000007855 methylation-specific PCR Methods 0.000 claims 4
- 239000000872 buffer Substances 0.000 claims 3
- 229920000136 polysorbate Polymers 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- 230000006326 desulfonation Effects 0.000 claims 2
- 238000005869 desulfonation reaction Methods 0.000 claims 2
- 239000000975 dye Substances 0.000 claims 2
- 239000012530 fluid Substances 0.000 claims 2
- 239000007850 fluorescent dye Substances 0.000 claims 2
- 239000011159 matrix material Substances 0.000 claims 2
- 239000011535 reaction buffer Substances 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- AOSFMYBATFLTAQ-UHFFFAOYSA-N 1-amino-3-(benzimidazol-1-yl)propan-2-ol Chemical compound C1=CC=C2N(CC(O)CN)C=NC2=C1 AOSFMYBATFLTAQ-UHFFFAOYSA-N 0.000 claims 1
- 108010037497 3'-nucleotidase Proteins 0.000 claims 1
- 102000051388 ADAMTS1 Human genes 0.000 claims 1
- 108091005660 ADAMTS1 Proteins 0.000 claims 1
- 101710099902 Acid-sensing ion channel 2 Proteins 0.000 claims 1
- 102100022094 Acid-sensing ion channel 2 Human genes 0.000 claims 1
- 102100027265 Aldo-keto reductase family 1 member B1 Human genes 0.000 claims 1
- IWOZXAIRAFGIMA-UHFFFAOYSA-N C(C)O.S(=S)(=O)([O-])[O-].NC(=[NH2+])N.NC(=[NH2+])N Chemical compound C(C)O.S(=S)(=O)([O-])[O-].NC(=[NH2+])N.NC(=[NH2+])N IWOZXAIRAFGIMA-UHFFFAOYSA-N 0.000 claims 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims 1
- 102100034535 Histone H3.1 Human genes 0.000 claims 1
- 102100028404 Homeobox protein Hox-B4 Human genes 0.000 claims 1
- 101000836540 Homo sapiens Aldo-keto reductase family 1 member B1 Proteins 0.000 claims 1
- 101001067844 Homo sapiens Histone H3.1 Proteins 0.000 claims 1
- 101000839788 Homo sapiens Homeobox protein Hox-B4 Proteins 0.000 claims 1
- 101000580043 Homo sapiens Ras-specific guanine nucleotide-releasing factor 2 Proteins 0.000 claims 1
- 101000648534 Homo sapiens Transmembrane 6 superfamily member 1 Proteins 0.000 claims 1
- 102100025825 Methylated-DNA-protein-cysteine methyltransferase Human genes 0.000 claims 1
- 108060004795 Methyltransferase Proteins 0.000 claims 1
- 102000016397 Methyltransferase Human genes 0.000 claims 1
- -1 RASSF1A Proteins 0.000 claims 1
- 102100027555 Ras-specific guanine nucleotide-releasing factor 2 Human genes 0.000 claims 1
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- 102100028864 Transmembrane 6 superfamily member 1 Human genes 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- SWSSJIPVPWGWOQ-UHFFFAOYSA-N carbamimidoylazanium;ethanol;thiocyanate Chemical compound CCO.[S-]C#N.NC([NH3+])=N SWSSJIPVPWGWOQ-UHFFFAOYSA-N 0.000 claims 1
- YQRQRUBEHCXCPR-UHFFFAOYSA-M cesium hydrogen sulfite Chemical compound [Cs+].OS([O-])=O YQRQRUBEHCXCPR-UHFFFAOYSA-M 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 239000012149 elution buffer Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 108040008770 methylated-DNA-[protein]-cysteine S-methyltransferase activity proteins Proteins 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- DJEHXEMURTVAOE-UHFFFAOYSA-M potassium bisulfite Chemical compound [K+].OS([O-])=O DJEHXEMURTVAOE-UHFFFAOYSA-M 0.000 claims 1
- 229940099427 potassium bisulfite Drugs 0.000 claims 1
- 235000010259 potassium hydrogen sulphite Nutrition 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 claims 1
- 229940001584 sodium metabisulfite Drugs 0.000 claims 1
- 235000010262 sodium metabisulphite Nutrition 0.000 claims 1
- 238000005382 thermal cycling Methods 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022014857A JP7466580B2 (ja) | 2015-06-15 | 2022-02-02 | 自動反応カートリッジにおける、ＤＮＡメチル化の統合精製及び測定並びに突然変異及び／又はｍＲＮＡ発現レベルの同時測定 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562175916P | 2015-06-15 | 2015-06-15 | |
| US62/175,916 | 2015-06-15 | ||
| PCT/US2016/037422 WO2016205233A2 (en) | 2015-06-15 | 2016-06-14 | Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022014857A Division JP7466580B2 (ja) | 2015-06-15 | 2022-02-02 | 自動反応カートリッジにおける、ＤＮＡメチル化の統合精製及び測定並びに突然変異及び／又はｍＲＮＡ発現レベルの同時測定 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018524978A JP2018524978A (ja) | 2018-09-06 |
| JP2018524978A5 true JP2018524978A5 (https=) | 2019-07-11 |
| JP7020922B2 JP7020922B2 (ja) | 2022-02-16 |
Family
ID=56204035
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017564901A Active JP7020922B2 (ja) | 2015-06-15 | 2016-06-14 | 自動反応カートリッジにおける、ＤＮＡメチル化の統合精製及び測定並びに突然変異及び／又はｍＲＮＡ発現レベルの同時測定 |
| JP2022014857A Active JP7466580B2 (ja) | 2015-06-15 | 2022-02-02 | 自動反応カートリッジにおける、ＤＮＡメチル化の統合精製及び測定並びに突然変異及び／又はｍＲＮＡ発現レベルの同時測定 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022014857A Active JP7466580B2 (ja) | 2015-06-15 | 2022-02-02 | 自動反応カートリッジにおける、ＤＮＡメチル化の統合精製及び測定並びに突然変異及び／又はｍＲＮＡ発現レベルの同時測定 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11603555B2 (https=) |
| EP (2) | EP3307885B1 (https=) |
| JP (2) | JP7020922B2 (https=) |
| CN (1) | CN107922941A (https=) |
| AU (3) | AU2016277943B2 (https=) |
| CA (1) | CA2989573A1 (https=) |
| ES (1) | ES2842205T3 (https=) |
| WO (1) | WO2016205233A2 (https=) |
Families Citing this family (33)
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| WO2016205233A2 (en) | 2015-06-15 | 2016-12-22 | Cepheid | Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
| DE102015009187B3 (de) * | 2015-07-16 | 2016-10-13 | Dimo Dietrich | Verfahren zur Bestimmung einer Mutation in genomischer DNA, Verwendung des Verfahrens und Kit zur Durchführung des Verfahrens |
| AU2016298048B2 (en) * | 2015-07-24 | 2022-08-04 | Cepheid | Compositions and methods for DNA and RNA extraction from tissue samples |
| MX2019006628A (es) * | 2016-12-12 | 2019-11-12 | Cepheid | Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado. |
| CN108277274A (zh) * | 2017-01-03 | 2018-07-13 | 博尔诚（北京）科技有限公司 | 用于区分胰腺癌和慢性胰腺炎的组合物及其用途 |
| US20200010907A1 (en) * | 2017-03-17 | 2020-01-09 | Mdxhealth Sa | Mgmt epigenetic deep-sequencing assay |
| CN107164535A (zh) * | 2017-07-07 | 2017-09-15 | 沈阳宁沪科技有限公司 | 一种无创高通量甲基化结肠癌诊断、研究和治疗方法 |
| US11466323B2 (en) * | 2017-07-13 | 2022-10-11 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Dual-probe digital droplet PCR strategy for specific detection of tissue-specific circulating DNA molecules |
| US11207674B2 (en) | 2017-08-09 | 2021-12-28 | Biogx, Inc. | Method of customizing a universal reagent cartridge with a lyophilized target-specific reagent |
| SG11202001209QA (en) * | 2017-08-25 | 2020-03-30 | Sekisui Medical Co Ltd | Chromatography packing for separation and/or detection of methylated dna |
| CN107868813B (zh) * | 2017-11-16 | 2021-07-27 | 上海覃谷生物技术有限公司 | 一种血浆dna边提取边转化的方法及应用 |
| CN119753091A (zh) | 2018-04-02 | 2025-04-04 | 格里尔公司 | 用于扩增与癌症相关联的cfDNA分子的化验板 |
| CN108300787A (zh) * | 2018-04-17 | 2018-07-20 | 中国科学院北京基因组研究所 | 特异甲基化位点作为乳腺癌早期诊断标志物的应用 |
| CN108531595A (zh) * | 2018-04-25 | 2018-09-14 | 新疆医科大学附属肿瘤医院 | 检测待测dna甲基化的引物组合物、检测方法及应用 |
| CN108456721B (zh) * | 2018-05-16 | 2021-07-09 | 基因科技（上海）股份有限公司 | 同步检测基因突变和甲基化的方法及其应用 |
| CN108676879A (zh) * | 2018-05-24 | 2018-10-19 | 中国科学院北京基因组研究所 | 特异甲基化位点作为乳腺癌分子分型诊断标志物的应用 |
| CN108796080B (zh) * | 2018-06-06 | 2022-06-07 | 苏州唯善生物科技有限公司 | 用于结直肠癌诊断、检测或筛查的引物和探针组 |
| CN108753979B (zh) * | 2018-07-09 | 2021-10-26 | 安徽达健医学科技有限公司 | 一种用于肝癌早期筛查的试剂盒及其使用方法 |
| CN112585282A (zh) * | 2018-07-26 | 2021-03-30 | 北京艾克伦医疗科技有限公司 | 鉴定乳腺癌状态的方法和试剂盒 |
| IL281741B2 (en) | 2018-09-27 | 2026-04-01 | Grail Inc | Methylation markers and a targeted methylation detection panel |
| CN110964813B (zh) * | 2018-09-29 | 2021-11-16 | 广州康立明生物科技股份有限公司 | Hoxa7甲基化检测试剂在制备肺癌诊断试剂中的用途 |
| CN111088351A (zh) * | 2018-10-23 | 2020-05-01 | 博尔诚（北京）科技有限公司 | 用于检测肺癌的组合物及其用途 |
| WO2020252721A1 (en) * | 2019-06-20 | 2020-12-24 | The Johns Hopkins University | Dna methylation markers and their use in differentiation of suspected cancerous lymph node biopsy samples |
| WO2020257404A1 (en) * | 2019-06-20 | 2020-12-24 | The Johns Hopkins University | Dna markers for differentiation of biopsy samples |
| CN111676287B (zh) * | 2020-06-03 | 2022-04-29 | 广州康立明生物科技股份有限公司 | 一种基因标志物组合及其应用 |
| CN111662980A (zh) * | 2020-06-03 | 2020-09-15 | 广州市康立明生物科技有限责任公司 | 一种肺癌检测试剂及试剂盒 |
| CN113930499B (zh) * | 2020-06-29 | 2023-09-22 | 武汉艾米森生命科技有限公司 | 检测基因甲基化的试剂的应用以及结直肠癌诊断或辅助诊断试剂盒 |
| CN114085894A (zh) * | 2021-11-03 | 2022-02-25 | 翌圣生物科技(上海)股份有限公司 | 核酸甲基化胞嘧啶转化方法 |
| WO2023133533A2 (en) * | 2022-01-06 | 2023-07-13 | The University Of Chicago | Methods and compositions for rapid detection and analysis of rna and dna cytosine methylation |
| DE102022204638B4 (de) | 2022-05-12 | 2025-01-02 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und mikrofluidische Vorrichtung zur Denaturierung einer Nukleinsäure |
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| MX2019006628A (es) | 2016-12-12 | 2019-11-12 | Cepheid | Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado. |
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2016
- 2016-06-14 WO PCT/US2016/037422 patent/WO2016205233A2/en not_active Ceased
- 2016-06-14 JP JP2017564901A patent/JP7020922B2/ja active Active
- 2016-06-14 CA CA2989573A patent/CA2989573A1/en active Pending
- 2016-06-14 CN CN201680048155.XA patent/CN107922941A/zh active Pending
- 2016-06-14 EP EP16732159.5A patent/EP3307885B1/en active Active
- 2016-06-14 EP EP20202659.7A patent/EP3839047A1/en active Pending
- 2016-06-14 ES ES16732159T patent/ES2842205T3/es active Active
- 2016-06-14 AU AU2016277943A patent/AU2016277943B2/en active Active
- 2016-06-14 US US15/182,394 patent/US11603555B2/en active Active
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2022
- 2022-02-02 JP JP2022014857A patent/JP7466580B2/ja active Active
- 2022-11-30 AU AU2022279465A patent/AU2022279465B2/en active Active
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2023
- 2023-01-11 US US18/095,982 patent/US20240141415A1/en active Pending
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2025
- 2025-06-16 AU AU2025204463A patent/AU2025204463A1/en active Pending
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