JP7020922B2 - 自動反応カートリッジにおける、DNAメチル化の統合精製及び測定並びに突然変異及び/又はmRNA発現レベルの同時測定 - Google Patents

自動反応カートリッジにおける、DNAメチル化の統合精製及び測定並びに突然変異及び/又はmRNA発現レベルの同時測定 Download PDF

Info

Publication number
JP7020922B2
JP7020922B2 JP2017564901A JP2017564901A JP7020922B2 JP 7020922 B2 JP7020922 B2 JP 7020922B2 JP 2017564901 A JP2017564901 A JP 2017564901A JP 2017564901 A JP2017564901 A JP 2017564901A JP 7020922 B2 JP7020922 B2 JP 7020922B2
Authority
JP
Japan
Prior art keywords
cartridge
methylation
dna
chamber
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017564901A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018524978A5 (https=
JP2018524978A (ja
Inventor
エドウィン・ダブリュー・ライ
アンドリュー・コールウェイ
リウエル・ヴァナッタ
ラッセル・ヒグチ
アレクサンダー・ゴール
クリステン・コックモンド
Original Assignee
セファイド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56204035&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP7020922(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by セファイド filed Critical セファイド
Publication of JP2018524978A publication Critical patent/JP2018524978A/ja
Publication of JP2018524978A5 publication Critical patent/JP2018524978A5/ja
Priority to JP2022014857A priority Critical patent/JP7466580B2/ja
Application granted granted Critical
Publication of JP7020922B2 publication Critical patent/JP7020922B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087Multiple sequential chambers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/154Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
JP2017564901A 2015-06-15 2016-06-14 自動反応カートリッジにおける、DNAメチル化の統合精製及び測定並びに突然変異及び/又はmRNA発現レベルの同時測定 Active JP7020922B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022014857A JP7466580B2 (ja) 2015-06-15 2022-02-02 自動反応カートリッジにおける、DNAメチル化の統合精製及び測定並びに突然変異及び/又はmRNA発現レベルの同時測定

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562175916P 2015-06-15 2015-06-15
US62/175,916 2015-06-15
PCT/US2016/037422 WO2016205233A2 (en) 2015-06-15 2016-06-14 Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022014857A Division JP7466580B2 (ja) 2015-06-15 2022-02-02 自動反応カートリッジにおける、DNAメチル化の統合精製及び測定並びに突然変異及び/又はmRNA発現レベルの同時測定

Publications (3)

Publication Number Publication Date
JP2018524978A JP2018524978A (ja) 2018-09-06
JP2018524978A5 JP2018524978A5 (https=) 2019-07-11
JP7020922B2 true JP7020922B2 (ja) 2022-02-16

Family

ID=56204035

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017564901A Active JP7020922B2 (ja) 2015-06-15 2016-06-14 自動反応カートリッジにおける、DNAメチル化の統合精製及び測定並びに突然変異及び/又はmRNA発現レベルの同時測定
JP2022014857A Active JP7466580B2 (ja) 2015-06-15 2022-02-02 自動反応カートリッジにおける、DNAメチル化の統合精製及び測定並びに突然変異及び/又はmRNA発現レベルの同時測定

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2022014857A Active JP7466580B2 (ja) 2015-06-15 2022-02-02 自動反応カートリッジにおける、DNAメチル化の統合精製及び測定並びに突然変異及び/又はmRNA発現レベルの同時測定

Country Status (8)

Country Link
US (2) US11603555B2 (https=)
EP (2) EP3307885B1 (https=)
JP (2) JP7020922B2 (https=)
CN (1) CN107922941A (https=)
AU (3) AU2016277943B2 (https=)
CA (1) CA2989573A1 (https=)
ES (1) ES2842205T3 (https=)
WO (1) WO2016205233A2 (https=)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10233440B2 (en) 2012-09-28 2019-03-19 Cepheid Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
WO2016205233A2 (en) 2015-06-15 2016-12-22 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
DE102015009187B3 (de) * 2015-07-16 2016-10-13 Dimo Dietrich Verfahren zur Bestimmung einer Mutation in genomischer DNA, Verwendung des Verfahrens und Kit zur Durchführung des Verfahrens
AU2016298048B2 (en) * 2015-07-24 2022-08-04 Cepheid Compositions and methods for DNA and RNA extraction from tissue samples
MX2019006628A (es) * 2016-12-12 2019-11-12 Cepheid Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado.
CN108277274A (zh) * 2017-01-03 2018-07-13 博尔诚(北京)科技有限公司 用于区分胰腺癌和慢性胰腺炎的组合物及其用途
US20200010907A1 (en) * 2017-03-17 2020-01-09 Mdxhealth Sa Mgmt epigenetic deep-sequencing assay
CN107164535A (zh) * 2017-07-07 2017-09-15 沈阳宁沪科技有限公司 一种无创高通量甲基化结肠癌诊断、研究和治疗方法
US11466323B2 (en) * 2017-07-13 2022-10-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Dual-probe digital droplet PCR strategy for specific detection of tissue-specific circulating DNA molecules
US11207674B2 (en) 2017-08-09 2021-12-28 Biogx, Inc. Method of customizing a universal reagent cartridge with a lyophilized target-specific reagent
SG11202001209QA (en) * 2017-08-25 2020-03-30 Sekisui Medical Co Ltd Chromatography packing for separation and/or detection of methylated dna
CN107868813B (zh) * 2017-11-16 2021-07-27 上海覃谷生物技术有限公司 一种血浆dna边提取边转化的方法及应用
CN119753091A (zh) 2018-04-02 2025-04-04 格里尔公司 用于扩增与癌症相关联的cfDNA分子的化验板
CN108300787A (zh) * 2018-04-17 2018-07-20 中国科学院北京基因组研究所 特异甲基化位点作为乳腺癌早期诊断标志物的应用
CN108531595A (zh) * 2018-04-25 2018-09-14 新疆医科大学附属肿瘤医院 检测待测dna甲基化的引物组合物、检测方法及应用
CN108456721B (zh) * 2018-05-16 2021-07-09 基因科技(上海)股份有限公司 同步检测基因突变和甲基化的方法及其应用
CN108676879A (zh) * 2018-05-24 2018-10-19 中国科学院北京基因组研究所 特异甲基化位点作为乳腺癌分子分型诊断标志物的应用
CN108796080B (zh) * 2018-06-06 2022-06-07 苏州唯善生物科技有限公司 用于结直肠癌诊断、检测或筛查的引物和探针组
CN108753979B (zh) * 2018-07-09 2021-10-26 安徽达健医学科技有限公司 一种用于肝癌早期筛查的试剂盒及其使用方法
CN112585282A (zh) * 2018-07-26 2021-03-30 北京艾克伦医疗科技有限公司 鉴定乳腺癌状态的方法和试剂盒
IL281741B2 (en) 2018-09-27 2026-04-01 Grail Inc Methylation markers and a targeted methylation detection panel
CN110964813B (zh) * 2018-09-29 2021-11-16 广州康立明生物科技股份有限公司 Hoxa7甲基化检测试剂在制备肺癌诊断试剂中的用途
CN111088351A (zh) * 2018-10-23 2020-05-01 博尔诚(北京)科技有限公司 用于检测肺癌的组合物及其用途
WO2020252721A1 (en) * 2019-06-20 2020-12-24 The Johns Hopkins University Dna methylation markers and their use in differentiation of suspected cancerous lymph node biopsy samples
WO2020257404A1 (en) * 2019-06-20 2020-12-24 The Johns Hopkins University Dna markers for differentiation of biopsy samples
CN111676287B (zh) * 2020-06-03 2022-04-29 广州康立明生物科技股份有限公司 一种基因标志物组合及其应用
CN111662980A (zh) * 2020-06-03 2020-09-15 广州市康立明生物科技有限责任公司 一种肺癌检测试剂及试剂盒
CN113930499B (zh) * 2020-06-29 2023-09-22 武汉艾米森生命科技有限公司 检测基因甲基化的试剂的应用以及结直肠癌诊断或辅助诊断试剂盒
CN114085894A (zh) * 2021-11-03 2022-02-25 翌圣生物科技(上海)股份有限公司 核酸甲基化胞嘧啶转化方法
WO2023133533A2 (en) * 2022-01-06 2023-07-13 The University Of Chicago Methods and compositions for rapid detection and analysis of rna and dna cytosine methylation
DE102022204638B4 (de) 2022-05-12 2025-01-02 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und mikrofluidische Vorrichtung zur Denaturierung einer Nukleinsäure
EP4299764A1 (en) * 2022-06-28 2024-01-03 Universal Diagnostics, S.A. Methods for detecting pancreatic cancer using dna methylation markers
WO2024111038A1 (ja) * 2022-11-22 2024-05-30 株式会社日立ハイテク 変異遺伝子検出方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009519023A (ja) 2005-12-14 2009-05-14 エフ.ホフマン−ラ ロシュ アーゲー 重亜硫酸処理のための新規方法
JP2009236933A (ja) 1997-12-24 2009-10-15 Cepheid 一体型流体操作カートリッジ

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5449602A (en) 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
EP2332958B1 (en) 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Detection of nucleic and sequence differences using the ligase detection reaction with addressable arrays
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
EP1736554B1 (en) 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US5958349A (en) 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
US6251594B1 (en) * 1997-06-09 2001-06-26 Usc/Norris Comprehensive Cancer Ctr. Cancer diagnostic method based upon DNA methylation differences
US6027998A (en) 1997-12-17 2000-02-22 Advanced Micro Devices, Inc. Method for fully planarized conductive line for a stack gate
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6942771B1 (en) * 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US6818185B1 (en) 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
JP4078073B2 (ja) 1999-05-28 2008-04-23 シーフィード 流体試料分析装置および方法
US6403037B1 (en) 2000-02-04 2002-06-11 Cepheid Reaction vessel and temperature control system
EP1313880A2 (en) 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US20060134643A1 (en) * 2000-06-19 2006-06-22 Kurt Berlin Bisulfite conversion of DNA
DE10112515B4 (de) 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
JP2003144172A (ja) * 2001-11-16 2003-05-20 Nisshinbo Ind Inc メチル化検出用オリゴヌクレオチド固定化基板
US9394332B2 (en) 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
WO2005042713A2 (en) 2003-10-28 2005-05-12 The Johns Hopkins University Quantitative multiplex methylation-specific pcr
EP2261650A3 (en) 2004-09-15 2011-07-06 IntegenX Inc. Microfluidic devices
US20060068399A1 (en) 2004-09-24 2006-03-30 Cepheid Multiple bead reagent system for protein based assays with optimized matrices
ES2647483T3 (es) * 2005-04-01 2017-12-21 Epigenomics Ag Conversión por bisulfito mejorada de ADN
US20060286577A1 (en) * 2005-06-17 2006-12-21 Xiyu Jia Methods for detection of methylated DNA
ES2373686T3 (es) 2005-06-23 2012-02-07 Biocartis Sa Cartucho, sistema y procedimiento para diagnósticos médicos automatizados.
US8703476B2 (en) * 2005-06-30 2014-04-22 Biocartis Sa Cartridge for automated medical diagnostics
JP5063616B2 (ja) * 2006-02-03 2012-10-31 インテジェニックス インコーポレイテッド マイクロ流体デバイス
US20090036665A1 (en) * 2007-07-11 2009-02-05 Gonzalo Domingo Disposable Sample Processing Unit
CN101802223A (zh) 2007-08-15 2010-08-11 香港大学 用于高通量亚硫酸氢盐dna-测序的方法和组合物及其用途
KR20110030415A (ko) 2008-01-22 2011-03-23 인터젠엑스 인크. 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도
FR2928659B1 (fr) * 2008-03-12 2012-01-13 Biomerieux Sa Procede pour le diagnostic ou pronostic in vitro du cancer du testicule
CN101285096A (zh) 2008-06-03 2008-10-15 中国人民解放军第二军医大学 一种快速检测dna甲基化的方法
US20120252015A1 (en) * 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
EP2218793A1 (en) 2009-02-13 2010-08-18 Alphagenics International SA Detection of MGMT methylation in tumors
CN102612652B (zh) * 2009-08-06 2016-05-04 康奈尔大学 用于分子分析的设备和方法
PL2539450T3 (pl) * 2010-02-25 2016-08-31 Advanced Liquid Logic Inc Sposób wytwarzania bibliotek kwasu nukleinowego
GB201004147D0 (en) 2010-03-12 2010-04-28 Dna Electronics Ltd Method and apparatus for sensing methylation
CN101984069B (zh) 2010-09-19 2012-07-25 生工生物工程(上海)有限公司 一种快速检测dna甲基化的试剂盒及方法
US20150136604A1 (en) * 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10132728B2 (en) 2012-04-27 2018-11-20 Cepheid Apparatus with heterogeneous processing modules
WO2013177265A1 (en) 2012-05-22 2013-11-28 The Johns Hopkins University A QUANTITATIVE MULTIPLEX METHYLATION SPECIFIC PCR METHOD- cMethDNA, REAGENTS, AND ITS USE
US20150154352A1 (en) * 2012-06-21 2015-06-04 Gigagen, Inc. System and Methods for Genetic Analysis of Mixed Cell Populations
CN104737024B (zh) * 2012-09-05 2018-01-09 塞弗德公司 流体分析系统中的通用对接站和数据门
US10233440B2 (en) 2012-09-28 2019-03-19 Cepheid Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
US20140272967A1 (en) 2013-03-13 2014-09-18 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
EP2971171A4 (en) * 2013-03-14 2016-11-02 Abbott Molecular Inc SYSTEMS AND METHODS FOR MULTIPLEX AMPLIFICATION SPECIFIC TO METHYLATION
US9670479B2 (en) 2013-03-15 2017-06-06 F Cubed, LLC Sample preparation device and methods of use
EP2971032B1 (en) * 2013-03-15 2019-05-08 Abbott Molecular Inc. Compositions and methods for nucleic acid extraction
GB201316347D0 (en) 2013-09-13 2013-10-30 Cancer Rec Tech Ltd Biological fluid filtration assembly
KR20150038944A (ko) 2013-10-01 2015-04-09 주식회사 바이오이즈 유전자의 메틸화 여부 및 비율의 분석방법
US20150099670A1 (en) * 2013-10-07 2015-04-09 Weiwei Li Method of preparing post-bisulfite conversion DNA library
ES2815684T3 (es) 2015-06-10 2021-03-30 Biocartis N V Detección mejorada de ADN metilado
WO2016205233A2 (en) 2015-06-15 2016-12-22 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
MX2019006628A (es) 2016-12-12 2019-11-12 Cepheid Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009236933A (ja) 1997-12-24 2009-10-15 Cepheid 一体型流体操作カートリッジ
JP2009519023A (ja) 2005-12-14 2009-05-14 エフ.ホフマン−ラ ロシュ アーゲー 重亜硫酸処理のための新規方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Bailey, V. J. et al.,Single-tube analysis of DNA methylation with silica superparamagnetic beads,Clin. Chem.,2010年,Vol. 56(6),pp. 1022-1025
High Pure PCR Product Purification Kit,ロシュ・ダイアグノスティックス株式会社,2005年01月
Holmes, E. E. et al.,Performance Evaluation of Kits for Bisulfite-Conversion of DNA from Tissues, Cell Lines, FFPE Tissues, Aspirates, Lavages, Effusions, Plasma, Serum, and Urine,PLoS One,2014年,Vol. 9(4): e93933,pp. 1-15

Also Published As

Publication number Publication date
ES2842205T3 (es) 2021-07-13
WO2016205233A2 (en) 2016-12-22
JP7466580B2 (ja) 2024-04-12
AU2025204463A1 (en) 2025-07-03
CA2989573A1 (en) 2016-12-22
AU2016277943A1 (en) 2018-02-01
EP3307885A2 (en) 2018-04-18
AU2016277943B2 (en) 2022-09-01
JP2018524978A (ja) 2018-09-06
US11603555B2 (en) 2023-03-14
US20170137871A1 (en) 2017-05-18
EP3307885B1 (en) 2020-10-21
EP3839047A1 (en) 2021-06-23
AU2022279465A1 (en) 2023-02-02
WO2016205233A3 (en) 2017-02-02
JP2022070885A (ja) 2022-05-13
AU2022279465B2 (en) 2025-04-10
CN107922941A (zh) 2018-04-17
US20240141415A1 (en) 2024-05-02

Similar Documents

Publication Publication Date Title
JP7466580B2 (ja) 自動反応カートリッジにおける、DNAメチル化の統合精製及び測定並びに突然変異及び/又はmRNA発現レベルの同時測定
US12539510B2 (en) Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or mRNA expression levels in an automated reaction cartridge
EP3942068B1 (en) Methods and systems for detecting methylation changes in dna samples
JP2020513801A (ja) メチル化状態が維持されるdna増幅方法
CN106687600A (zh) 用于甲基化分析的方法
JP2020534820A (ja) メチル化分析のための方法
CN102016067A (zh) 前列腺癌中gstp1高甲基化的检测
US20130309667A1 (en) Primers for analyzing methylated sequences and methods of use thereof
CN111349691B (zh) Egfr基因缺失突变检测用组合物、试剂盒及检测方法
RU2780660C2 (ru) ОБЪЕДИНЕННАЯ ОЧИСТКА И ИЗМЕРЕНИЕ МЕТИЛИРОВАНИЯ ДНК С СОВМЕСТНЫМ ИЗМЕРЕНИЕМ МУТАЦИЙ И/ИЛИ УРОВНЕЙ ЭКСПРЕССИИ мРНК В АВТОМАТИЧЕСКОМ РЕАКЦИОННОМ КАРТРИДЖЕ
HK40013640A (en) Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
BR112019011670B1 (pt) Conjunto de cartuchos e método para determinação do estado de metilação de um ácido nucleico, sistema para determinação da metilação de um ácido nucleico em uma amostra biológica, método para detecção de um câncer ou da predisposição a um câncer em um indivíduo, e kit para determinação de metilação do dna
US20130310550A1 (en) Primers for analyzing methylated sequences and methods of use thereof
HK40067347A (en) Methods and systems for detecting methylation changes in dna samples
HK40067347B (en) Methods and systems for detecting methylation changes in dna samples

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190610

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190610

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200713

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20201012

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210113

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210621

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210921

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211214

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20220104

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20220203

R150 Certificate of patent or registration of utility model

Ref document number: 7020922

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250