JP2020532964A5 - - Google Patents
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- JP2020532964A5 JP2020532964A5 JP2020509067A JP2020509067A JP2020532964A5 JP 2020532964 A5 JP2020532964 A5 JP 2020532964A5 JP 2020509067 A JP2020509067 A JP 2020509067A JP 2020509067 A JP2020509067 A JP 2020509067A JP 2020532964 A5 JP2020532964 A5 JP 2020532964A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- mainstream
- primers
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 33
- 108020004707 nucleic acids Proteins 0.000 claims 32
- 102000039446 nucleic acids Human genes 0.000 claims 32
- 150000007523 nucleic acids Chemical class 0.000 claims 32
- 239000002773 nucleotide Substances 0.000 claims 12
- 125000003729 nucleotide group Chemical group 0.000 claims 12
- 239000000523 sample Substances 0.000 claims 9
- 208000036878 aneuploidy Diseases 0.000 claims 8
- 231100001075 aneuploidy Toxicity 0.000 claims 8
- 230000003321 amplification Effects 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 108020004414 DNA Proteins 0.000 claims 5
- 230000002759 chromosomal effect Effects 0.000 claims 5
- 210000000349 chromosome Anatomy 0.000 claims 5
- 230000001605 fetal effect Effects 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 3
- 108091093088 Amplicon Proteins 0.000 claims 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 2
- 210000002593 Y chromosome Anatomy 0.000 claims 2
- 230000005856 abnormality Effects 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 238000011304 droplet digital PCR Methods 0.000 claims 2
- 230000002068 genetic effect Effects 0.000 claims 2
- 238000009830 intercalation Methods 0.000 claims 2
- 230000008774 maternal effect Effects 0.000 claims 2
- 239000002751 oligonucleotide probe Substances 0.000 claims 2
- 108091061744 Cell-free fetal DNA Proteins 0.000 claims 1
- 201000010374 Down Syndrome Diseases 0.000 claims 1
- 201000006360 Edwards syndrome Diseases 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 208000016679 Monosomy X Diseases 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 201000009928 Patau syndrome Diseases 0.000 claims 1
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims 1
- 206010044686 Trisomy 13 Diseases 0.000 claims 1
- 208000006284 Trisomy 13 Syndrome Diseases 0.000 claims 1
- 208000007159 Trisomy 18 Syndrome Diseases 0.000 claims 1
- 206010044688 Trisomy 21 Diseases 0.000 claims 1
- 208000026928 Turner syndrome Diseases 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 210000000601 blood cell Anatomy 0.000 claims 1
- 210000001124 body fluid Anatomy 0.000 claims 1
- 239000010839 body fluid Substances 0.000 claims 1
- 239000002299 complementary DNA Substances 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 238000007847 digital PCR Methods 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 238000011901 isothermal amplification Methods 0.000 claims 1
- 238000011880 melting curve analysis Methods 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 210000004940 nucleus Anatomy 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
- 206010053884 trisomy 18 Diseases 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762544605P | 2017-08-11 | 2017-08-11 | |
| US62/544,605 | 2017-08-11 | ||
| PCT/US2018/046360 WO2019033065A1 (en) | 2017-08-11 | 2018-08-10 | DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDE COMPOSITION PRIMERS |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020532964A JP2020532964A (ja) | 2020-11-19 |
| JP2020532964A5 true JP2020532964A5 (https=) | 2021-09-02 |
| JP7537746B2 JP7537746B2 (ja) | 2024-08-21 |
Family
ID=65271769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020509067A Active JP7537746B2 (ja) | 2017-08-11 | 2018-08-10 | ヌクレオチド組成が限定されたプライマーを用いたデジタル増幅 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11739375B2 (https=) |
| EP (1) | EP3665297A4 (https=) |
| JP (1) | JP7537746B2 (https=) |
| CN (1) | CN111183229A (https=) |
| AU (1) | AU2018313286B2 (https=) |
| CA (1) | CA3072591A1 (https=) |
| WO (1) | WO2019033065A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2983819A1 (en) * | 2015-04-24 | 2016-10-27 | Atila Biosystems Incorporated | Amplification with primers of limited nucleotide composition |
| WO2019033065A1 (en) | 2017-08-11 | 2019-02-14 | Atila Biosystems, Inc. | DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDE COMPOSITION PRIMERS |
| CN109666747A (zh) * | 2019-02-20 | 2019-04-23 | 天津脉络医学检验有限公司 | 一种引物探针组合物及其应用 |
| IT201900006679A1 (it) * | 2019-05-09 | 2020-11-09 | Artemisia S P A | METODO PER LA DETERMINAZIONE DIRETTA DI ANEUPLOIDIE A CARICO DEL FETO DA ANALISI NON INVASIVA DEL DNA FETALE DA SANGUE MATERNO MEDIANTE dPCR |
| CN110656167A (zh) * | 2019-09-27 | 2020-01-07 | 中国科学院苏州生物医学工程技术研究所 | 用于y染色体微缺失检测的引物组、试剂盒及基于数字pcr的检测方法 |
| CN110904182B (zh) * | 2019-11-19 | 2023-08-18 | 中国科学院深圳先进技术研究院 | 基因组重复序列删除率确定方法、装置、设备及存储介质 |
| CN111500788B (zh) * | 2020-05-29 | 2023-11-07 | 领航医学科技(深圳)有限公司 | 用于人类疱疹病毒感染检测的试剂盒及其检测方法 |
| CN112899346A (zh) * | 2021-01-05 | 2021-06-04 | 南京普济生物有限公司 | 一种降低pcr非特异性扩增的核苷酸及其设计方法与应用 |
| CN112899366A (zh) * | 2021-01-06 | 2021-06-04 | 南京普济生物有限公司 | 一种结直肠癌基因突变位点的检测方法及其试剂盒 |
| US12252745B2 (en) * | 2021-09-02 | 2025-03-18 | Enumerix, Inc. | Detection and digital quantitation of multiple targets |
| CN113960142B (zh) * | 2021-10-25 | 2023-11-10 | 福州大学 | 一种回文核酸纳米片超灵敏电化学生物传感器的制备方法 |
| CN113789389A (zh) * | 2021-11-18 | 2021-12-14 | 广东永诺医疗科技有限公司 | 人白血病bcr-abl融合突变一步法检测试剂盒 |
| CN114196740A (zh) * | 2021-12-09 | 2022-03-18 | 南京普济生物有限公司 | 用于同时识别多种基因类型的数字扩增检测方法、检测产品和检测试剂盒 |
| US20260110031A1 (en) * | 2022-10-14 | 2026-04-23 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for evaluating health and stability of cultured cells |
| CN118853760A (zh) * | 2024-07-15 | 2024-10-29 | 首都医科大学附属北京天坛医院 | 一种构建Map3k3I441M突变敲入转基因小鼠模型的方法及应用 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| DE10236406C1 (de) | 2002-08-02 | 2003-12-24 | Epigenomics Ag | Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität |
| EP1604040B1 (en) | 2003-03-07 | 2010-10-13 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process |
| US7387876B2 (en) | 2004-02-27 | 2008-06-17 | President And Fellows Of Harvard College | Amplification of trace amounts of nucleic acids |
| US8871469B1 (en) | 2004-11-13 | 2014-10-28 | Steven Albert Benner | Self-avoiding molecular recognition systems in DNA priming |
| US7833942B2 (en) | 2004-12-03 | 2010-11-16 | Human Genetic Signatures Pty. Ltd. | Methods for simplifying microbial nucleic acids by chemical modification of cytosines |
| EP3591068A1 (en) * | 2006-02-02 | 2020-01-08 | The Board of Trustees of the Leland Stanford Junior University | Non-invasive fetal genetic screening by digital analysis |
| US20090036315A1 (en) | 2006-02-07 | 2009-02-05 | Antara Biosciences Inc. | Device and methods for detecting and quantifying one or more target agents |
| KR101110013B1 (ko) | 2007-10-05 | 2012-02-29 | (주)바이오니아 | 서열 내에 어베이직 부분을 포함하는 ｐｃｒ 증폭용프라이머 |
| KR20100019220A (ko) | 2008-08-08 | 2010-02-18 | 삼성전자주식회사 | 열순환을 포함하는 다중 치환 증폭에 의하여 표적 핵산 서열을 증폭하는 방법 |
| FR2940805B1 (fr) | 2009-01-05 | 2015-10-16 | Biomerieux Sa | Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede |
| EP3460074B1 (en) | 2010-02-05 | 2020-10-07 | Quest Diagnostics Investments Incorporated | Method to detect repeat sequence motifs in nucleic acid |
| SG10201605049QA (en) * | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| US9034584B2 (en) | 2011-07-29 | 2015-05-19 | Cellular Dynamics International, Inc. | Metabolic maturation in stem cell-derived tissue cells |
| EP2785839A2 (en) | 2011-11-30 | 2014-10-08 | University of Bremen | Expression of mirnas in placental tissue |
| US20140065613A1 (en) | 2012-09-06 | 2014-03-06 | Life Technologies Corporation | Multiplex Y-STR Analysis |
| US20140274811A1 (en) | 2013-03-14 | 2014-09-18 | Lyle J. Arnold | Methods for Amplifying a Complete Genome or Transcriptome |
| WO2014201254A1 (en) | 2013-06-12 | 2014-12-18 | University Of Washington Through Its Center For Commercialization | Methods for maturing cardiomyocytes and uses thereof |
| HRP20210953T1 (hr) | 2014-02-18 | 2021-09-03 | Illumina, Inc. | Postupci i pripravci za određivanje dnk profila |
| US10612080B2 (en) * | 2014-09-22 | 2020-04-07 | Roche Molecular Systems, Inc. | Digital PCR for non-invasive prenatal testing |
| CA2983819A1 (en) | 2015-04-24 | 2016-10-27 | Atila Biosystems Incorporated | Amplification with primers of limited nucleotide composition |
| WO2019033065A1 (en) | 2017-08-11 | 2019-02-14 | Atila Biosystems, Inc. | DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDE COMPOSITION PRIMERS |
-
2018
- 2018-08-10 WO PCT/US2018/046360 patent/WO2019033065A1/en not_active Ceased
- 2018-08-10 CN CN201880064757.3A patent/CN111183229A/zh active Pending
- 2018-08-10 EP EP18844988.8A patent/EP3665297A4/en active Pending
- 2018-08-10 JP JP2020509067A patent/JP7537746B2/ja active Active
- 2018-08-10 CA CA3072591A patent/CA3072591A1/en active Pending
- 2018-08-10 US US16/638,346 patent/US11739375B2/en active Active
- 2018-08-10 AU AU2018313286A patent/AU2018313286B2/en active Active
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