JP2020503858A5 - - Google Patents
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- Publication number
- JP2020503858A5 JP2020503858A5 JP2019531197A JP2019531197A JP2020503858A5 JP 2020503858 A5 JP2020503858 A5 JP 2020503858A5 JP 2019531197 A JP2019531197 A JP 2019531197A JP 2019531197 A JP2019531197 A JP 2019531197A JP 2020503858 A5 JP2020503858 A5 JP 2020503858A5
- Authority
- JP
- Japan
- Prior art keywords
- cartridge
- dna
- cartridges
- methylation
- chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims 24
- 239000003153 chemical reaction reagent Substances 0.000 claims 18
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 14
- 230000011987 methylation Effects 0.000 claims 12
- 238000007069 methylation reaction Methods 0.000 claims 12
- 239000002773 nucleotide Substances 0.000 claims 10
- 125000003729 nucleotide group Chemical group 0.000 claims 10
- 238000006243 chemical reaction Methods 0.000 claims 9
- 238000000034 method Methods 0.000 claims 9
- 239000011159 matrix material Substances 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 150000007523 nucleic acids Chemical class 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 229940079826 hydrogen sulfite Drugs 0.000 claims 7
- 206010028980 Neoplasm Diseases 0.000 claims 6
- 201000011510 cancer Diseases 0.000 claims 6
- 238000007855 methylation-specific PCR Methods 0.000 claims 5
- 239000000203 mixture Substances 0.000 claims 5
- 239000012472 biological sample Substances 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims 3
- 230000006326 desulfonation Effects 0.000 claims 3
- 238000005869 desulfonation reaction Methods 0.000 claims 3
- 229910052739 hydrogen Inorganic materials 0.000 claims 3
- 239000001257 hydrogen Substances 0.000 claims 3
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 3
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims 3
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 claims 2
- ZETCGWYACBNPIH-UHFFFAOYSA-N azane;sulfurous acid Chemical compound N.OS(O)=O ZETCGWYACBNPIH-UHFFFAOYSA-N 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 239000003054 catalyst Substances 0.000 claims 2
- 238000007857 nested PCR Methods 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 239000002516 radical scavenger Substances 0.000 claims 2
- 239000011535 reaction buffer Substances 0.000 claims 2
- 238000005382 thermal cycling Methods 0.000 claims 2
- 230000007067 DNA methylation Effects 0.000 claims 1
- 102100025825 Methylated-DNA-protein-cysteine methyltransferase Human genes 0.000 claims 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims 1
- GLEVLJDDWXEYCO-UHFFFAOYSA-N Trolox Chemical compound O1C(C)(C(O)=O)CCC2=C1C(C)=C(C)C(O)=C2C GLEVLJDDWXEYCO-UHFFFAOYSA-N 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- YQRQRUBEHCXCPR-UHFFFAOYSA-M cesium hydrogen sulfite Chemical compound [Cs+].OS([O-])=O YQRQRUBEHCXCPR-UHFFFAOYSA-M 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 238000002844 melting Methods 0.000 claims 1
- 230000008018 melting Effects 0.000 claims 1
- 108040008770 methylated-DNA-[protein]-cysteine S-methyltransferase activity proteins Proteins 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 229920000768 polyamine Polymers 0.000 claims 1
- DJEHXEMURTVAOE-UHFFFAOYSA-M potassium bisulfite Chemical compound [K+].OS([O-])=O DJEHXEMURTVAOE-UHFFFAOYSA-M 0.000 claims 1
- 235000010259 potassium hydrogen sulphite Nutrition 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 claims 1
- 229940001584 sodium metabisulfite Drugs 0.000 claims 1
- 235000010262 sodium metabisulphite Nutrition 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662433165P | 2016-12-12 | 2016-12-12 | |
| US62/433,165 | 2016-12-12 | ||
| PCT/US2017/065905 WO2018111935A1 (en) | 2016-12-12 | 2017-12-12 | Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020503858A JP2020503858A (ja) | 2020-02-06 |
| JP2020503858A5 true JP2020503858A5 (https=) | 2021-01-28 |
| JP7123050B2 JP7123050B2 (ja) | 2022-08-22 |
Family
ID=60888716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019531197A Active JP7123050B2 (ja) | 2016-12-12 | 2017-12-12 | 自動反応カートリッジにおける統合された、ＤＮＡメチル化の精製及び測定並びに変異及び／又はｍＲＮＡ発現レベルの同時測定 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US11260387B2 (https=) |
| EP (1) | EP3551332B1 (https=) |
| JP (1) | JP7123050B2 (https=) |
| KR (1) | KR102479432B1 (https=) |
| CN (1) | CN110267744A (https=) |
| AU (1) | AU2017376118B2 (https=) |
| CA (1) | CA3046636A1 (https=) |
| MX (1) | MX2019006628A (https=) |
| WO (1) | WO2018111935A1 (https=) |
| ZA (1) | ZA201903750B (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016205233A2 (en) | 2015-06-15 | 2016-12-22 | Cepheid | Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
| MX2019006628A (es) | 2016-12-12 | 2019-11-12 | Cepheid | Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado. |
| US11207674B2 (en) * | 2017-08-09 | 2021-12-28 | Biogx, Inc. | Method of customizing a universal reagent cartridge with a lyophilized target-specific reagent |
| CN108913777B (zh) * | 2018-08-16 | 2022-10-18 | 上海市公共卫生临床中心 | 诊断肿瘤的dna甲基化相关的标记物及其应用 |
| CN112176419B (zh) * | 2019-10-16 | 2022-03-22 | 中国医学科学院肿瘤医院 | 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法 |
| CN111500731A (zh) * | 2020-05-20 | 2020-08-07 | 深圳市新合生物医疗科技有限公司 | 一种甲状腺癌早期诊断、检测或者筛查的试剂盒及其使用方法、应用 |
| CN111662980A (zh) * | 2020-06-03 | 2020-09-15 | 广州市康立明生物科技有限责任公司 | 一种肺癌检测试剂及试剂盒 |
| CN111676287B (zh) * | 2020-06-03 | 2022-04-29 | 广州康立明生物科技股份有限公司 | 一种基因标志物组合及其应用 |
| CN111705130B (zh) * | 2020-06-03 | 2022-07-12 | 广州康立明生物科技股份有限公司 | 一种基因标志物组合及其应用 |
| CN120082430A (zh) * | 2020-06-19 | 2025-06-03 | 中国疾病预防控制中心病毒病预防控制所 | 一种样品处理及检测装置 |
| CN113832141B (zh) * | 2021-09-30 | 2024-03-08 | 苏州唯善生物科技有限公司 | 粪便人源基因组dna特异性纯化方法及试剂盒 |
| CN113699242A (zh) * | 2021-10-18 | 2021-11-26 | 浙江省人民医院 | 检测kras基因突变、adamts1与bnc1甲基化的引物探针，试剂盒与方法 |
| WO2023069022A2 (en) | 2021-10-21 | 2023-04-27 | Star Array Pte. Ltd. | Device and method for processing biological samples |
| CN115961045A (zh) * | 2022-12-28 | 2023-04-14 | 泰州竹石医学检验实验室有限公司 | 用于判断早期肝细胞癌的肿瘤标志物、试剂盒及其使用方法 |
| US12024739B1 (en) * | 2023-03-15 | 2024-07-02 | Dnanudge Limited | Method and apparatus for rapid analysis of a biological sample |
Family Cites Families (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1190838A (en) | 1981-07-17 | 1985-07-23 | Cavit Akin | Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer |
| US5449602A (en) | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| EP2332958B1 (en) | 1996-02-09 | 2016-04-20 | Cornell Research Foundation, Inc. | Detection of nucleic and sequence differences using the ligase detection reaction with addressable arrays |
| EP0892808B1 (en) | 1996-04-12 | 2008-05-14 | PHRI Properties, Inc. | Detection probes, kits and assays |
| EP1736554B1 (en) | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US6312892B1 (en) | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| US5958349A (en) | 1997-02-28 | 1999-09-28 | Cepheid | Reaction vessel for heat-exchanging chemical processes |
| US6251594B1 (en) | 1997-06-09 | 2001-06-26 | Usc/Norris Comprehensive Cancer Ctr. | Cancer diagnostic method based upon DNA methylation differences |
| CA2305613A1 (en) * | 1997-10-22 | 1999-04-29 | Safety Associates, Inc. | Methods and apparatus for determining analytes in various matrices |
| US6027998A (en) | 1997-12-17 | 2000-02-22 | Advanced Micro Devices, Inc. | Method for fully planarized conductive line for a stack gate |
| DK1179585T3 (da) | 1997-12-24 | 2008-11-10 | Cepheid | Indretning og fremgangsmåde til lysis |
| US6506594B1 (en) | 1999-03-19 | 2003-01-14 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US6942771B1 (en) | 1999-04-21 | 2005-09-13 | Clinical Micro Sensors, Inc. | Microfluidic systems in the electrochemical detection of target analytes |
| JP4078073B2 (ja) | 1999-05-28 | 2008-04-23 | シーフィード | 流体試料分析装置および方法 |
| US6818185B1 (en) | 1999-05-28 | 2004-11-16 | Cepheid | Cartridge for conducting a chemical reaction |
| US6403037B1 (en) | 2000-02-04 | 2002-06-11 | Cepheid | Reaction vessel and temperature control system |
| EP1313880A2 (en) | 2000-05-30 | 2003-05-28 | PE Corporation (NY) | Methods for detecting target nucleic acids using coupled ligation and amplification |
| US6605451B1 (en) | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| US20060134643A1 (en) | 2000-06-19 | 2006-06-22 | Kurt Berlin | Bisulfite conversion of DNA |
| DE10112515B4 (de) | 2001-03-09 | 2004-02-12 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
| US9394332B2 (en) | 2002-08-29 | 2016-07-19 | Epigenomics Ag | Method for bisulfite treatment |
| US7288373B2 (en) | 2003-05-02 | 2007-10-30 | Human Genetic Signatures Pty Ltd. | Treatment of methylated nucleic acid |
| WO2005042713A2 (en) | 2003-10-28 | 2005-05-12 | The Johns Hopkins University | Quantitative multiplex methylation-specific pcr |
| EP2261650A3 (en) * | 2004-09-15 | 2011-07-06 | IntegenX Inc. | Microfluidic devices |
| US20060068399A1 (en) | 2004-09-24 | 2006-03-30 | Cepheid | Multiple bead reagent system for protein based assays with optimized matrices |
| ES2647483T3 (es) | 2005-04-01 | 2017-12-21 | Epigenomics Ag | Conversión por bisulfito mejorada de ADN |
| US20060286577A1 (en) | 2005-06-17 | 2006-12-21 | Xiyu Jia | Methods for detection of methylated DNA |
| ES2373686T3 (es) | 2005-06-23 | 2012-02-07 | Biocartis Sa | Cartucho, sistema y procedimiento para diagnósticos médicos automatizados. |
| US8703476B2 (en) | 2005-06-30 | 2014-04-22 | Biocartis Sa | Cartridge for automated medical diagnostics |
| EP1963352B1 (en) | 2005-12-14 | 2010-09-08 | Roche Diagnostics GmbH | New method for bisulfite treatment |
| JP5063616B2 (ja) | 2006-02-03 | 2012-10-31 | インテジェニックス インコーポレイテッド | マイクロ流体デバイス |
| US20090036665A1 (en) | 2007-07-11 | 2009-02-05 | Gonzalo Domingo | Disposable Sample Processing Unit |
| CN101802223A (zh) | 2007-08-15 | 2010-08-11 | 香港大学 | 用于高通量亚硫酸氢盐dna-测序的方法和组合物及其用途 |
| KR20110030415A (ko) * | 2008-01-22 | 2011-03-23 | 인터젠엑스 인크. | 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도 |
| FR2928659B1 (fr) | 2008-03-12 | 2012-01-13 | Biomerieux Sa | Procede pour le diagnostic ou pronostic in vitro du cancer du testicule |
| CN101285096A (zh) | 2008-06-03 | 2008-10-15 | 中国人民解放军第二军医大学 | 一种快速检测dna甲基化的方法 |
| US20120252015A1 (en) | 2011-02-18 | 2012-10-04 | Bio-Rad Laboratories | Methods and compositions for detecting genetic material |
| EP2218793A1 (en) | 2009-02-13 | 2010-08-18 | Alphagenics International SA | Detection of MGMT methylation in tumors |
| GB201004147D0 (en) * | 2010-03-12 | 2010-04-28 | Dna Electronics Ltd | Method and apparatus for sensing methylation |
| CN101984069B (zh) | 2010-09-19 | 2012-07-25 | 生工生物工程(上海)有限公司 | 一种快速检测dna甲基化的试剂盒及方法 |
| US10132728B2 (en) * | 2012-04-27 | 2018-11-20 | Cepheid | Apparatus with heterogeneous processing modules |
| WO2013177265A1 (en) | 2012-05-22 | 2013-11-28 | The Johns Hopkins University | A QUANTITATIVE MULTIPLEX METHYLATION SPECIFIC PCR METHOD- cMethDNA, REAGENTS, AND ITS USE |
| CN104737024B (zh) | 2012-09-05 | 2018-01-09 | 塞弗德公司 | 流体分析系统中的通用对接站和数据门 |
| US10233440B2 (en) | 2012-09-28 | 2019-03-19 | Cepheid | Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples |
| US20140272967A1 (en) | 2013-03-13 | 2014-09-18 | Abbott Molecular Inc. | Systems and methods for isolating nucleic acids |
| EP2971171A4 (en) | 2013-03-14 | 2016-11-02 | Abbott Molecular Inc | SYSTEMS AND METHODS FOR MULTIPLEX AMPLIFICATION SPECIFIC TO METHYLATION |
| US9670479B2 (en) | 2013-03-15 | 2017-06-06 | F Cubed, LLC | Sample preparation device and methods of use |
| GB201316347D0 (en) | 2013-09-13 | 2013-10-30 | Cancer Rec Tech Ltd | Biological fluid filtration assembly |
| KR20150038944A (ko) * | 2013-10-01 | 2015-04-09 | 주식회사 바이오이즈 | 유전자의 메틸화 여부 및 비율의 분석방법 |
| US20150099670A1 (en) | 2013-10-07 | 2015-04-09 | Weiwei Li | Method of preparing post-bisulfite conversion DNA library |
| ES2815684T3 (es) | 2015-06-10 | 2021-03-30 | Biocartis N V | Detección mejorada de ADN metilado |
| WO2016205233A2 (en) | 2015-06-15 | 2016-12-22 | Cepheid | Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
| MX2019006628A (es) | 2016-12-12 | 2019-11-12 | Cepheid | Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado. |
-
2017
- 2017-12-12 MX MX2019006628A patent/MX2019006628A/es unknown
- 2017-12-12 EP EP17822953.0A patent/EP3551332B1/en active Active
- 2017-12-12 KR KR1020197019958A patent/KR102479432B1/ko active Active
- 2017-12-12 WO PCT/US2017/065905 patent/WO2018111935A1/en not_active Ceased
- 2017-12-12 JP JP2019531197A patent/JP7123050B2/ja active Active
- 2017-12-12 AU AU2017376118A patent/AU2017376118B2/en active Active
- 2017-12-12 CA CA3046636A patent/CA3046636A1/en active Pending
- 2017-12-12 US US15/839,731 patent/US11260387B2/en active Active
- 2017-12-12 CN CN201780085871.XA patent/CN110267744A/zh active Pending
-
2019
- 2019-06-11 ZA ZA2019/03750A patent/ZA201903750B/en unknown
-
2022
- 2022-01-27 US US17/586,645 patent/US12539510B2/en active Active
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