MX2019006628A - Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado. - Google Patents

Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado.

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Publication number
MX2019006628A
MX2019006628A MX2019006628A MX2019006628A MX2019006628A MX 2019006628 A MX2019006628 A MX 2019006628A MX 2019006628 A MX2019006628 A MX 2019006628A MX 2019006628 A MX2019006628 A MX 2019006628A MX 2019006628 A MX2019006628 A MX 2019006628A
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Mexico
Prior art keywords
nucleic acid
dna
matrix material
measurement
deaminated
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Application number
MX2019006628A
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English (en)
Inventor
Wei- Lung Lai Edwin
Kohlway Andrew
Van Atta Reuel
Higuchi Russell
A Gall Alexander
Kocmond Kriszten
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Cepheid
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Publication date
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Publication of MX2019006628A publication Critical patent/MX2019006628A/es

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Abstract

Se proporcionan métodos para determinar la metilación de ADN. En una modalidad ilustrativa, pero no limitante, el método comprende i) poner en contacto una muestra biológica que comprende un ácido nucleico con un primer material de matriz que comprende una primera columna o filtro donde el material de matriz se une y/o filtra ácidos nucleicos en la muestra y por lo tanto, purifica el ADN; ii) eluir el ADN unido del primer material de la matriz y desnaturalizar el ADN para producir ADN desnaturalizado eluido; iii) calentar el ADN eluido en presencia de iones bisulfito para producir un ácido nucleico desaminado; iv) poner en contacto el ácido nucleico desaminado con un segundo material de matriz que comprende una segunda columna para unir este ácido nucleico desaminado con este segundo material de matriz; v) desulfonar el ácido nucleico desaminado unido y/o simultáneamente eluir y desulfonar el ácido nucleico poniendo en contacto el ácido nucleico desaminado con una solución alcalina para producir un ácido nucleico convertido con bisulfito; vi) eluir este ácido nucleico convertido con bisulfito del segundo material de matriz; y vii) realizar la PCR específica de metilación y/o secuenciación de ácido nucleico, y/o análisis de fusión de alta resolución (HRM) en el ácido nucleico convertido con bisulfito para determinar la metilación del ácido nucleico, en donde al menos los pasos iv) a vi) se realizan en un solo cartucho de reacción.
MX2019006628A 2016-12-12 2017-12-12 Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado. MX2019006628A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662433165P 2016-12-12 2016-12-12
PCT/US2017/065905 WO2018111935A1 (en) 2016-12-12 2017-12-12 Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge

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MX2019006628A true MX2019006628A (es) 2019-11-12

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MX2019006628A MX2019006628A (es) 2016-12-12 2017-12-12 Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado.

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US (2) US11260387B2 (es)
EP (1) EP3551332B1 (es)
JP (1) JP7123050B2 (es)
KR (1) KR102479432B1 (es)
CN (1) CN110267744A (es)
AU (1) AU2017376118B2 (es)
BR (1) BR112019011670A2 (es)
CA (1) CA3046636A1 (es)
MX (1) MX2019006628A (es)
WO (1) WO2018111935A1 (es)
ZA (1) ZA201903750B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016205233A2 (en) 2015-06-15 2016-12-22 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
EP3551332B1 (en) 2016-12-12 2024-01-10 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
US20190134634A1 (en) * 2017-08-09 2019-05-09 Shazi Iqbal Reagent Storage Device for Storing a Target-Specific Reagent
CN108913777B (zh) * 2018-08-16 2022-10-18 上海市公共卫生临床中心 诊断肿瘤的dna甲基化相关的标记物及其应用
CN112176419B (zh) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法
CN111500731A (zh) * 2020-05-20 2020-08-07 深圳市新合生物医疗科技有限公司 一种甲状腺癌早期诊断、检测或者筛查的试剂盒及其使用方法、应用
CN111705130B (zh) * 2020-06-03 2022-07-12 广州康立明生物科技股份有限公司 一种基因标志物组合及其应用
CN111662980A (zh) * 2020-06-03 2020-09-15 广州市康立明生物科技有限责任公司 一种肺癌检测试剂及试剂盒
EP4170008A1 (en) * 2020-06-19 2023-04-26 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention Sample processing and detection apparatus and application thereof
CN113832141B (zh) * 2021-09-30 2024-03-08 苏州唯善生物科技有限公司 粪便人源基因组dna特异性纯化方法及试剂盒
CN113699242A (zh) * 2021-10-18 2021-11-26 浙江省人民医院 检测kras基因突变、adamts1与bnc1甲基化的引物探针，试剂盒与方法
WO2023069022A2 (en) 2021-10-21 2023-04-27 Star Array Pte. Ltd. Device and method for processing biological samples

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5449602A (en) 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2244891C (en) 1996-02-09 2008-12-30 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
JP3898228B2 (ja) 1996-04-12 2007-03-28 ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク 検出プローブ、キット及びアッセイ
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US5958349A (en) 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
AU7829398A (en) 1997-06-09 1998-12-30 University Of Southern California A cancer diagnostic method based upon dna methylation differences
US6440725B1 (en) * 1997-12-24 2002-08-27 Cepheid Integrated fluid manipulation cartridge
US6027998A (en) 1997-12-17 2000-02-22 Advanced Micro Devices, Inc. Method for fully planarized conductive line for a stack gate
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6818185B1 (en) 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
JP4022069B2 (ja) 1999-05-28 2007-12-12 シーフィード 細胞破壊装置および方法
US6403037B1 (en) 2000-02-04 2002-06-11 Cepheid Reaction vessel and temperature control system
AU2001265121A1 (en) 2000-05-30 2001-12-11 Applera Corporation Methods for detecting target nucleic acids using coupled ligation and amplification
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
DE10112515B4 (de) 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
AU2003236461B2 (en) 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
US7288373B2 (en) 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
KR101214780B1 (ko) * 2004-09-15 2012-12-21 인터젠엑스 인크. 미세유동 장치
US20060068399A1 (en) 2004-09-24 2006-03-30 Cepheid Multiple bead reagent system for protein based assays with optimized matrices
US8129107B2 (en) 2005-04-01 2012-03-06 Epigenomics Ag Bisulfite conversion of DNA
US20060286577A1 (en) 2005-06-17 2006-12-21 Xiyu Jia Methods for detection of methylated DNA
RU2432205C2 (ru) 2005-06-23 2011-10-27 Биокартис С.А. Картридж, система и способ автоматизированной медицинской диагностики
ATE529186T1 (de) 2005-06-30 2011-11-15 Biocartis Sa Kassette zur automatisierten medizinischen diagnose
US8137937B2 (en) 2005-12-14 2012-03-20 Roche Molecular Systems, Inc. Method for bisulfite treatment
KR20080096567A (ko) 2006-02-03 2008-10-30 마이크로칩 바이오테크놀로지스, 인크. 마이크로유체 장치
US20090036665A1 (en) 2007-07-11 2009-02-05 Gonzalo Domingo Disposable Sample Processing Unit
WO2009024019A1 (en) 2007-08-15 2009-02-26 The University Of Hong Kong Methods and compositions for high-throughput bisulphite dna-sequencing and utilities
CN101990516B (zh) * 2008-01-22 2015-09-09 英特基因有限公司 多用试样准备系统及其在集成分析系统中的使用
FR2928659B1 (fr) 2008-03-12 2012-01-13 Biomerieux Sa Procede pour le diagnostic ou pronostic in vitro du cancer du testicule
CN101285096A (zh) * 2008-06-03 2008-10-15 中国人民解放军第二军医大学 一种快速检测dna甲基化的方法
US20120252015A1 (en) 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
EP2218793A1 (en) 2009-02-13 2010-08-18 Alphagenics International SA Detection of MGMT methylation in tumors
GB201004147D0 (en) * 2010-03-12 2010-04-28 Dna Electronics Ltd Method and apparatus for sensing methylation
CN101984069B (zh) 2010-09-19 2012-07-25 生工生物工程(上海)有限公司 一种快速检测dna甲基化的试剂盒及方法
KR102145460B1 (ko) * 2012-04-27 2020-08-18 세페이드 이질적 처리 모듈을 갖는 장치
EP3828270A1 (en) 2012-09-28 2021-06-02 Cepheid Compositions for dna and rna extraction from fixed paraffin-embedded tissue samples
WO2014160233A1 (en) 2013-03-13 2014-10-02 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
US9701999B2 (en) 2013-03-14 2017-07-11 Abbott Molecular, Inc. Multiplex methylation-specific amplification systems and methods
US9670479B2 (en) 2013-03-15 2017-06-06 F Cubed, LLC Sample preparation device and methods of use
GB201316347D0 (en) 2013-09-13 2013-10-30 Cancer Rec Tech Ltd Biological fluid filtration assembly
KR20150038944A (ko) * 2013-10-01 2015-04-09 주식회사 바이오이즈 유전자의 메틸화 여부 및 비율의 분석방법
US20150099670A1 (en) 2013-10-07 2015-04-09 Weiwei Li Method of preparing post-bisulfite conversion DNA library
CN107667179B (zh) 2015-06-10 2021-04-13 拜奥卡蒂斯股份有限公司 改进的甲基化dna检测
WO2016205233A2 (en) * 2015-06-15 2016-12-22 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
EP3551332B1 (en) 2016-12-12 2024-01-10 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge

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US11260387B2 (en) 2022-03-01
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