BR112019011670A2 - purificação integrada e mensuração da metilação do dna e comensuração de mutações e/ou níveis de expressão de mrna em um cartucho de reação automático - Google Patents

purificação integrada e mensuração da metilação do dna e comensuração de mutações e/ou níveis de expressão de mrna em um cartucho de reação automático

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Publication number
BR112019011670A2
BR112019011670A2 BR112019011670A BR112019011670A BR112019011670A2 BR 112019011670 A2 BR112019011670 A2 BR 112019011670A2 BR 112019011670 A BR112019011670 A BR 112019011670A BR 112019011670 A BR112019011670 A BR 112019011670A BR 112019011670 A2 BR112019011670 A2 BR 112019011670A2
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Brazil
Prior art keywords
nucleic acid
matrix material
dna
commensuration
dna methylation
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BR112019011670A
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English (en)
Inventor
A Gall Alexander
Kohlway Andrew
Wei-Lung Lai Edwin
Kocmond Kriszten
Van Atta Reuel
Higuchi Russell
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Cepheid
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Publication date
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Publication of BR112019011670A2 publication Critical patent/BR112019011670A2/pt

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    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases

Abstract

métodos para determinação da metilação do dna são fornecidos. em uma ilustrativa, mas não limitante modalidade, o método compreende i) colocar uma amostra biológica compreendendo um ácido nucleico, em contato com um primeiro material de matriz compreendendo uma primeira coluna ou filtro, em que o referido material de matriz liga-se e/ou filtra ácidos nucleicos na referida amostra, purificando, assim, o dna; ii) eluir o dna ligado proveniente do primeiro material de matriz e desnaturar o dna para produzir dna desnaturado eluído; iii) aquecer o dna eluído na presença de íons bissulfito para produzir um ácido nucleico desaminado; iv) colocar o referido ácido nucleico desaminado em contato com um segundo material de matriz compreendendo uma segunda coluna para ligar o referido ácido nucleico desaminado ao referido segundo material de matriz; v) dessulfonar o ácido nucleico desaminado ligado e/ou eluir e dessulfonar simultaneamente o ácido nucleico colocando o ácido nucleico desaminado em contato com uma solução alcalina para produzir um ácido nucleico convertido com bissulfito; vi) eluir o referido ácido nucleico convertido com bissulfito do referido segundo material de matriz.
BR112019011670A 2016-12-12 2017-12-12 purificação integrada e mensuração da metilação do dna e comensuração de mutações e/ou níveis de expressão de mrna em um cartucho de reação automático BR112019011670A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662433165P 2016-12-12 2016-12-12
PCT/US2017/065905 WO2018111935A1 (en) 2016-12-12 2017-12-12 Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge

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BR112019011670A2 true BR112019011670A2 (pt) 2019-10-22

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US (2) US11260387B2 (pt)
EP (1) EP3551332B1 (pt)
JP (1) JP7123050B2 (pt)
KR (1) KR102479432B1 (pt)
CN (1) CN110267744A (pt)
AU (1) AU2017376118B2 (pt)
BR (1) BR112019011670A2 (pt)
CA (1) CA3046636A1 (pt)
MX (1) MX2019006628A (pt)
WO (1) WO2018111935A1 (pt)
ZA (1) ZA201903750B (pt)

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CN110267744A (zh) 2019-09-20
RU2019119086A (ru) 2021-01-12
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RU2019119086A3 (pt) 2021-05-07
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JP2020503858A (ja) 2020-02-06
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