JP2014503207A5 - - Google Patents
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- JP2014503207A5 JP2014503207A5 JP2013544833A JP2013544833A JP2014503207A5 JP 2014503207 A5 JP2014503207 A5 JP 2014503207A5 JP 2013544833 A JP2013544833 A JP 2013544833A JP 2013544833 A JP2013544833 A JP 2013544833A JP 2014503207 A5 JP2014503207 A5 JP 2014503207A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- template
- strand
- reverse
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 150000007523 nucleic acids Chemical class 0.000 claims 21
- 238000000034 method Methods 0.000 claims 20
- 108020004707 nucleic acids Proteins 0.000 claims 16
- 102000039446 nucleic acids Human genes 0.000 claims 16
- 230000000295 complement effect Effects 0.000 claims 13
- 108091093088 Amplicon Proteins 0.000 claims 8
- 230000003321 amplification Effects 0.000 claims 6
- 239000007791 liquid phase Substances 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 4
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 4
- 239000011324 bead Substances 0.000 claims 3
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 2
- 229930024421 Adenine Natural products 0.000 claims 2
- 102000053642 Catalytic RNA Human genes 0.000 claims 2
- 108090000994 Catalytic RNA Proteins 0.000 claims 2
- 229960000643 adenine Drugs 0.000 claims 2
- 238000004873 anchoring Methods 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 238000002844 melting Methods 0.000 claims 2
- 230000008018 melting Effects 0.000 claims 2
- 108091008146 restriction endonucleases Proteins 0.000 claims 2
- 108091092562 ribozyme Proteins 0.000 claims 2
- 229940113082 thymine Drugs 0.000 claims 2
- 229940035893 uracil Drugs 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 238000003491 array Methods 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000001419 dependent effect Effects 0.000 claims 1
- 238000010348 incorporation Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 230000004807 localization Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000012071 phase Substances 0.000 claims 1
- 230000002285 radioactive effect Effects 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201061424599P | 2010-12-17 | 2010-12-17 | |
| US61/424,599 | 2010-12-17 | ||
| US201161445324P | 2011-02-22 | 2011-02-22 | |
| US61/445,324 | 2011-02-22 | ||
| US201161451919P | 2011-03-11 | 2011-03-11 | |
| US61/451,919 | 2011-03-11 | ||
| US201161526478P | 2011-08-23 | 2011-08-23 | |
| US61/526,478 | 2011-08-23 | ||
| US201161552660P | 2011-10-28 | 2011-10-28 | |
| US61/552,660 | 2011-10-28 | ||
| PCT/US2011/065535 WO2012083189A2 (en) | 2010-12-17 | 2011-12-16 | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014503207A JP2014503207A (ja) | 2014-02-13 |
| JP2014503207A5 true JP2014503207A5 (enExample) | 2014-11-06 |
| JP5907990B2 JP5907990B2 (ja) | 2016-05-11 |
Family
ID=45478545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013544833A Active JP5907990B2 (ja) | 2010-12-17 | 2011-12-16 | 核酸増幅のための方法、組成物、システム、装置およびキット |
Country Status (5)
| Country | Link |
|---|---|
| US (10) | US20120156728A1 (enExample) |
| EP (3) | EP2652148B1 (enExample) |
| JP (1) | JP5907990B2 (enExample) |
| CN (3) | CN110079588B (enExample) |
| WO (1) | WO2012083189A2 (enExample) |
Families Citing this family (158)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US20090047673A1 (en) | 2006-08-22 | 2009-02-19 | Cary Robert B | Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
| WO2009137059A1 (en) | 2008-05-05 | 2009-11-12 | Los Alamos National Security, Llc | Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control |
| US8583380B2 (en) | 2008-09-05 | 2013-11-12 | Aueon, Inc. | Methods for stratifying and annotating cancer drug treatment options |
| US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
| US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US10030045B2 (en) | 2010-02-19 | 2018-07-24 | Ohio State Innovation Foundation | Primers and methods for nucleic acid amplification |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12545960B2 (en) | 2010-05-18 | 2026-02-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| RU2565550C2 (ru) | 2010-09-24 | 2015-10-20 | Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити | Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров |
| NZ610129A (en) | 2010-10-04 | 2014-08-29 | Genapsys Inc | Systems and methods for automated reusable parallel biological reactions |
| US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
| US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
| CN110079588B (zh) | 2010-12-17 | 2024-03-15 | 生命技术公司 | 用于核酸扩增的方法、组合物、系统、仪器和试剂盒 |
| EP2656263B1 (en) | 2010-12-22 | 2019-11-06 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| ES2632768T3 (es) | 2011-01-17 | 2017-09-15 | Life Technologies Corporation | Flujo de trabajo para la detección de ligandos empleando ácidos nucleicos |
| EP3733867A1 (en) | 2011-01-17 | 2020-11-04 | Life Technologies Corporation | Enzymatic ligation of nucleic acids |
| JP6153874B2 (ja) | 2011-02-09 | 2017-06-28 | ナテラ， インコーポレイテッド | 非侵襲的出生前倍数性呼び出しのための方法 |
| EP2699698B9 (en) | 2011-04-20 | 2020-07-15 | Mesa Biotech, Inc. | Oscillating amplification reaction for nucleic acids |
| US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
| US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
| JP6193252B2 (ja) | 2011-12-01 | 2017-09-06 | ジナプシス インコーポレイテッド | 高効率電子配列決定及び検出のためのシステム並びに方法 |
| SG11201404243WA (en) | 2012-01-26 | 2014-08-28 | Nugen Technologies Inc | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
| CN104471075B (zh) | 2012-04-19 | 2018-06-22 | 生命技术公司 | 核酸扩增 |
| EP3095879B1 (en) | 2012-04-19 | 2018-09-19 | Life Technologies Corporation | Nucleic acid amplification |
| RU2014146286A (ru) | 2012-04-19 | 2016-06-10 | Лайф Текнолоджиз Корпорейшн | Способ осуществления цифровой пцр |
| JP6181751B2 (ja) | 2012-06-18 | 2017-08-16 | ニューゲン テクノロジーズ， インコーポレイテッド | 望まれない核酸配列のネガティブ選択のための組成物および方法 |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| AU2012385961B9 (en) * | 2012-07-24 | 2017-05-18 | Natera, Inc. | Highly multiplex PCR methods and compositions |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| EP2888371B1 (en) * | 2012-08-24 | 2017-08-02 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid paired end sequencing |
| WO2014043143A1 (en) | 2012-09-11 | 2014-03-20 | Life Technologies Corporation | Nucleic acid amplification |
| EP3257952B1 (en) | 2012-09-11 | 2020-02-12 | Life Technologies Corporation | Nucleic acid amplification |
| WO2014138385A1 (en) * | 2013-03-06 | 2014-09-12 | Ohio State Innovation Foundation | Isothermal amplification of nuleic acid, and library preparation and clone generation in sequencing |
| US10975423B2 (en) * | 2013-03-11 | 2021-04-13 | Elitechgroup, Inc. | Methods for true isothermal strand displacement amplification |
| WO2014164479A1 (en) * | 2013-03-11 | 2014-10-09 | Elitech Holding B.V. | Methods for true isothermal strand displacement amplification |
| US10590474B2 (en) * | 2013-03-11 | 2020-03-17 | Elitechgroup B.V. | Methods for true isothermal strand displacement amplification |
| EP3249057B1 (en) | 2013-03-14 | 2019-05-01 | Life Technologies Corporation | Matrix arrays and methods for making same |
| US9809852B2 (en) | 2013-03-15 | 2017-11-07 | Genapsys, Inc. | Systems and methods for biological analysis |
| WO2014144092A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Sequential sequencing |
| TWI707038B (zh) | 2013-08-05 | 2020-10-11 | 美商扭轉生物科技有限公司 | 重新合成之基因庫 |
| CA2929596C (en) | 2013-11-13 | 2022-07-05 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
| EP3792921A1 (en) | 2013-12-11 | 2021-03-17 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| WO2015161054A2 (en) | 2014-04-18 | 2015-10-22 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
| US12492429B2 (en) | 2014-04-21 | 2025-12-09 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| CN113774132A (zh) | 2014-04-21 | 2021-12-10 | 纳特拉公司 | 检测染色体片段中的突变和倍性 |
| US20150337295A1 (en) | 2014-05-08 | 2015-11-26 | Fluidigm Corporation | Integrated single cell sequencing |
| US20180173846A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| US20150361481A1 (en) | 2014-06-13 | 2015-12-17 | Life Technologies Corporation | Multiplex nucleic acid amplification |
| WO2016084489A1 (ja) | 2014-11-27 | 2016-06-02 | 株式会社日立ハイテクノロジーズ | スポットアレイ基板、その製造方法、核酸ポリマー解析方法及び装置 |
| WO2016126987A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Compositions and methods for synthetic gene assembly |
| WO2016126882A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| CN107250383B (zh) * | 2015-02-17 | 2022-09-06 | 深圳华大智造科技股份有限公司 | 使用受控的链置换的dna序列测定 |
| GB201502645D0 (en) * | 2015-02-17 | 2015-04-01 | Touchlight Genetics Ltd | Method |
| EP4095264A1 (en) | 2015-03-05 | 2022-11-30 | Life Technologies Corporation | Surface stabilization of biosensors |
| CN120425026A (zh) | 2015-04-10 | 2025-08-05 | 十程基因技术瑞典公司 | 生物样本的空间区别、多重核酸分析 |
| US9981239B2 (en) | 2015-04-21 | 2018-05-29 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| US11085073B2 (en) | 2015-04-24 | 2021-08-10 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on a solid support |
| AU2016253147B2 (en) | 2015-04-24 | 2021-07-22 | Mesa Biotech, Inc. | Fluidic test cassette |
| WO2016170179A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on solid support |
| BR112017023257A2 (pt) | 2015-04-29 | 2018-07-17 | Univ California | composições e métodos para construir bibliotecas de cdna de cadeias específicas |
| DK3294906T3 (en) | 2015-05-11 | 2024-08-05 | Natera Inc | Methods for determining ploidy |
| JP6609641B2 (ja) * | 2015-05-29 | 2019-11-20 | イルミナ ケンブリッジ リミテッド | クラスターにおける表面プライマーの高度な利用 |
| JP6698708B2 (ja) | 2015-06-09 | 2020-05-27 | ライフ テクノロジーズ コーポレーション | 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体 |
| KR20180050411A (ko) | 2015-09-18 | 2018-05-14 | 트위스트 바이오사이언스 코포레이션 | 올리고핵산 변이체 라이브러리 및 그의 합성 |
| CN108698012A (zh) | 2015-09-22 | 2018-10-23 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
| WO2017062343A1 (en) | 2015-10-06 | 2017-04-13 | Pierce Biotechnology, Inc. | Devices and methods for producing nucleic acids and proteins |
| WO2017095958A1 (en) | 2015-12-01 | 2017-06-08 | Twist Bioscience Corporation | Functionalized surfaces and preparation thereof |
| EP3848457B1 (en) | 2016-02-08 | 2025-08-13 | Rgene, Inc. | Multiple ligase compositions, systems, and methods |
| DK3443119T3 (da) | 2016-04-15 | 2022-05-23 | Natera Inc | Fremgangsmåde til bestemmelse af lunge cancer |
| JP2017209051A (ja) * | 2016-05-25 | 2017-11-30 | 株式会社ニコン | 標的生体分子の検出方法、標的生体分子検出用基板、及び標的生体分子検出装置 |
| AU2017278350B2 (en) | 2016-06-06 | 2023-10-19 | Redvault Biosciences, Lp | Target reporter constructs and uses thereof |
| WO2017214561A1 (en) | 2016-06-10 | 2017-12-14 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| GB201611469D0 (en) | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| US10544456B2 (en) | 2016-07-20 | 2020-01-28 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
| US10443098B2 (en) * | 2016-08-15 | 2019-10-15 | Omniome, Inc. | Method and system for sequencing nucleic acids |
| KR102212257B1 (ko) | 2016-08-22 | 2021-02-04 | 트위스트 바이오사이언스 코포레이션 | 드 노보 합성된 핵산 라이브러리 |
| WO2018057526A2 (en) | 2016-09-21 | 2018-03-29 | Twist Bioscience Corporation | Nucleic acid based data storage |
| US11345961B2 (en) * | 2016-10-05 | 2022-05-31 | Roche Sequencing Solutions, Inc. | Nucleic acid sequencing using nanotransistors |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| EP3554514A4 (en) | 2016-12-16 | 2020-08-05 | Twist Bioscience Corporation | BANKS OF VARIANTS OF IMMUNOLOGICAL SYNAPSE AND THEIR SYNTHESIS |
| CN109937259B (zh) * | 2017-01-10 | 2023-01-13 | 深圳华大智造科技股份有限公司 | 一种提高核酸聚合测序质量的方法、反应体系和试剂盒 |
| EP3929306A1 (en) | 2017-01-26 | 2021-12-29 | QIAGEN GmbH | Method for enriching template nucleic acids |
| CN110892485B (zh) | 2017-02-22 | 2024-03-22 | 特韦斯特生物科学公司 | 基于核酸的数据存储 |
| AU2018234629A1 (en) | 2017-03-15 | 2019-10-17 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| US10737267B2 (en) | 2017-04-04 | 2020-08-11 | Omniome, Inc. | Fluidic apparatus and methods useful for chemical and biological reactions |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| JP7169999B2 (ja) | 2017-06-12 | 2022-11-11 | ツイスト バイオサイエンス コーポレーション | シームレス核酸アセンブリのための方法 |
| SG11202000964UA (en) | 2017-08-15 | 2020-02-27 | Omniome Inc | Scanning apparatus and methods useful for detection of chemical and biological analytes |
| AU2018328847A1 (en) | 2017-09-11 | 2020-04-23 | Twist Bioscience Corporation | GPCR binding proteins and synthesis thereof |
| CA3076378A1 (en) | 2017-09-21 | 2019-03-28 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
| CN118048419A (zh) * | 2017-10-04 | 2024-05-17 | 生捷科技控股公司 | 酶法合成寡核苷酸的方法和系统 |
| CA3079411C (en) | 2017-10-19 | 2023-12-05 | Omniome, Inc. | Simultaneous background reduction and complex stabilization in binding assay workflows |
| WO2019079769A1 (en) | 2017-10-20 | 2019-04-25 | Twist Bioscience Corporation | HEATED NANOWELLS FOR THE SYNTHESIS OF POLYNUCLEOTIDES |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| US12226745B2 (en) | 2017-11-07 | 2025-02-18 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| WO2019094524A1 (en) | 2017-11-07 | 2019-05-16 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| WO2019118926A1 (en) | 2017-12-14 | 2019-06-20 | Tai Diagnostics, Inc. | Assessing graft suitability for transplantation |
| EP4715681A2 (en) | 2018-01-04 | 2026-03-25 | Atlas Data Storage, Inc. | Dna-based digital information storage |
| GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| GB201804585D0 (en) * | 2018-03-22 | 2018-05-09 | Dnae Diagnostics Ltd | Methods for amplication of nucleic acids with Endonuclease-Mediated shifting equilibrium amplification (EM-SEq) |
| DK3781714T3 (da) | 2018-04-14 | 2026-03-30 | Natera Inc | Fremgangsmåder til cancerpåvisning og -overvågning ved personlig påvisning af cirkulerende tumor-dna |
| CA3097583A1 (en) | 2018-04-19 | 2019-10-24 | Omniome, Inc. | Improving accuracy of base calls in nucleic acid sequencing methods |
| IL278771B2 (en) | 2018-05-18 | 2025-09-01 | Twist Bioscience Corp | Polynucleotides, reagents and methods for nucleic acid hybridization |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| WO2020018824A1 (en) * | 2018-07-19 | 2020-01-23 | Ultima Genomics, Inc. | Nucleic acid clonal amplification and sequencing methods, systems, and kits |
| CA3106410A1 (en) | 2018-07-23 | 2020-01-30 | Dna Script | Massively parallel enzymatic synthesis of nucleic acid strands |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| WO2020101795A1 (en) | 2018-11-15 | 2020-05-22 | Omniome, Inc. | Electronic detection of nucleic acid structure |
| CN211311438U (zh) | 2018-12-04 | 2020-08-21 | 欧姆尼欧美公司 | 流动池 |
| AU2019391274B2 (en) | 2018-12-05 | 2025-10-30 | Illumina Cambridge Limited | Methods and compositions for cluster generation by bridge amplification |
| US20220290217A1 (en) | 2018-12-10 | 2022-09-15 | 10X Genomics, Inc. | Resolving spatial arrays using deconvolution |
| JP7562424B2 (ja) * | 2018-12-17 | 2024-10-07 | イルミナ ケンブリッジ リミテッド | シーケンシング用プライマーオリゴヌクレオチド |
| JP7587425B2 (ja) | 2018-12-18 | 2024-11-20 | イルミナ ケンブリッジ リミテッド | 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物 |
| WO2020132350A2 (en) | 2018-12-20 | 2020-06-25 | Omniome, Inc. | Temperature control for analysis of nucleic acids and other analytes |
| WO2020139871A1 (en) | 2018-12-26 | 2020-07-02 | Twist Bioscience Corporation | Highly accurate de novo polynucleotide synthesis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| EP3924515A4 (en) | 2019-02-11 | 2022-08-24 | Ultima Genomics, Inc. | PROCEDURE FOR NUCLEIC ACID ANALYSIS |
| CN113613787B (zh) | 2019-02-20 | 2023-06-13 | 加利福尼亚太平洋生物科学股份有限公司 | 用于检测化学和生物分析物的扫描装置和方法 |
| AU2020227802A1 (en) | 2019-02-26 | 2021-10-14 | Twist Bioscience Corporation | Variant nucleic acid libraries for antibody optimization |
| WO2020176678A1 (en) | 2019-02-26 | 2020-09-03 | Twist Bioscience Corporation | Variant nucleic acid libraries for glp1 receptor |
| GB201905303D0 (en) | 2019-04-15 | 2019-05-29 | Thermo Fisher Scient Geneart Gmbh | Multiplex assembly of nucleic acid molecules |
| EP4403648B1 (en) | 2019-05-03 | 2025-08-20 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| WO2020252186A1 (en) | 2019-06-11 | 2020-12-17 | Omniome, Inc. | Calibrated focus sensing |
| CA3144644A1 (en) | 2019-06-21 | 2020-12-24 | Twist Bioscience Corporation | Barcode-based nucleic acid sequence assembly |
| US10656368B1 (en) | 2019-07-24 | 2020-05-19 | Omniome, Inc. | Method and system for biological imaging using a wide field objective lens |
| EP4407043A3 (en) | 2019-08-21 | 2024-10-09 | Life Technologies Corporation | System and method for sequencing |
| TW202124406A (zh) | 2019-09-10 | 2021-07-01 | 美商歐姆尼歐美公司 | 核苷酸之可逆修飾 |
| EP4034564A4 (en) | 2019-09-23 | 2023-12-13 | Twist Bioscience Corporation | VARIANT NUCLEIC ACID LIBRARIES FOR SINGLE-DOMAIN ANTIBODIES |
| US12091777B2 (en) | 2019-09-23 | 2024-09-17 | Twist Bioscience Corporation | Variant nucleic acid libraries for CRTH2 |
| BR112022011235A2 (pt) | 2019-12-09 | 2022-12-13 | Twist Bioscience Corp | Bibliotecas de variantes de ácido nucleico para receptores de adenosina |
| EP3851542A1 (en) | 2020-01-20 | 2021-07-21 | Tecan Genomics, Inc. | Depletion of abundant uninformative sequences |
| CN115362264A (zh) | 2020-01-29 | 2022-11-18 | 10X基因组学有限公司 | 用于分析物检测的组合物和方法 |
| US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| CN113275053A (zh) | 2020-02-03 | 2021-08-20 | 帝肯基因组学公司 | 试剂存储系统 |
| WO2021158511A1 (en) | 2020-02-04 | 2021-08-12 | Omniome, Inc. | Flow cells and methods for their manufacture and use |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| EP4243986A1 (en) | 2020-11-16 | 2023-09-20 | Life Technologies Corporation | System and method for sequencing |
| JP2024502618A (ja) | 2021-01-08 | 2024-01-22 | セラノーム， インコーポレイテッド | 生体試料を分析するためのデバイスおよび方法 |
| US12576400B2 (en) | 2021-01-08 | 2026-03-17 | Cellanome, Inc. | Methods for incubating and analyzing a cell in a compartment of a fluidic device |
| US11535892B1 (en) | 2021-06-17 | 2022-12-27 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| US11859241B2 (en) | 2021-06-17 | 2024-01-02 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| AU2023325064A1 (en) | 2022-08-15 | 2025-03-06 | Element Biosciences, Inc. | Spatially resolved surface capture of nucleic acids |
| GB202215624D0 (en) | 2022-10-21 | 2022-12-07 | Dnae Diagnostics Ltd | Clonal amplification |
| WO2026080643A2 (en) | 2024-10-08 | 2026-04-16 | Life Technologies Corporation | Systems and methods for detecting nucleotide incorporation |
Family Cites Families (105)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1009396A (en) | 1911-06-03 | 1911-11-21 | Arthur E Fahrman | Perforating-machine. |
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4988617A (en) | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| US5426180A (en) | 1991-03-27 | 1995-06-20 | Research Corporation Technologies, Inc. | Methods of making single-stranded circular oligonucleotides |
| WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| RU2048522C1 (ru) | 1992-10-14 | 1995-11-20 | Институт белка РАН | Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления |
| US6001568A (en) | 1992-10-26 | 1999-12-14 | Institut Belka | Solid medium for amplification and expression of nucleic acids as colonies |
| US5593826A (en) | 1993-03-22 | 1997-01-14 | Perkin-Elmer Corporation, Applied Biosystems, Inc. | Enzymatic ligation of 3'amino-substituted oligonucleotides |
| WO1994024143A1 (en) | 1993-04-12 | 1994-10-27 | Northwestern University | Method of forming oligonucleotides |
| SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| AT402203B (de) | 1995-06-13 | 1997-03-25 | Himmler Gottfried Dipl Ing Dr | Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5670325A (en) | 1996-08-14 | 1997-09-23 | Exact Laboratories, Inc. | Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample |
| EP2332957B1 (en) | 1996-02-09 | 2015-04-08 | Cornell Research Foundation, Inc. | Detection of nucleic and acid sequence differences using the ligase detection reaction with addressable arrays |
| US5928870A (en) | 1997-06-16 | 1999-07-27 | Exact Laboratories, Inc. | Methods for the detection of loss of heterozygosity |
| US6335164B1 (en) | 1996-08-29 | 2002-01-01 | Daikin Industries, Ltd. | Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using RecA-like recombinase |
| US5948653A (en) | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
| EP1591541B1 (en) | 1997-04-01 | 2012-02-15 | Illumina Cambridge Limited | Method of nucleic acid sequencing |
| CA2305449A1 (en) | 1997-10-10 | 1999-04-22 | President & Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US6306590B1 (en) | 1998-06-08 | 2001-10-23 | Caliper Technologies Corp. | Microfluidic matrix localization apparatus and methods |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| GB9902970D0 (en) | 1999-02-11 | 1999-03-31 | Zeneca Ltd | Novel matrix |
| US6531302B1 (en) * | 1999-04-12 | 2003-03-11 | Nanogen/Becton Dickinson Partnership | Anchored strand displacement amplification on an electronically addressable microchip |
| AUPQ008799A0 (en) * | 1999-04-30 | 1999-05-27 | Tillett, Daniel | Genome sequencing |
| US6300070B1 (en) * | 1999-06-04 | 2001-10-09 | Mosaic Technologies, Inc. | Solid phase methods for amplifying multiple nucleic acids |
| US6440706B1 (en) | 1999-08-02 | 2002-08-27 | Johns Hopkins University | Digital amplification |
| US6413792B1 (en) | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| EP1313880A2 (en) | 2000-05-30 | 2003-05-28 | PE Corporation (NY) | Methods for detecting target nucleic acids using coupled ligation and amplification |
| AR031640A1 (es) * | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
| KR20030082535A (ko) * | 2001-03-09 | 2003-10-22 | 뉴젠 테크놀로지스 인코포레이티드 | Ｒｎａ 서열의 증폭을 위한 방법 및 조성물 |
| DE60226856D1 (de) | 2001-04-20 | 2008-07-10 | Penn State Res Found | Verfahren zur manipulation von nukleinsäuren |
| AU2002258997A1 (en) | 2001-04-24 | 2002-11-05 | Li-Cor, Inc. | Polymerases with charge-switch activity and methods of generating such polymerases |
| US20040161741A1 (en) * | 2001-06-30 | 2004-08-19 | Elazar Rabani | Novel compositions and processes for analyte detection, quantification and amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| CA2476481C (en) | 2002-02-21 | 2016-01-26 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US20050118616A1 (en) * | 2002-08-16 | 2005-06-02 | Kawashima Tadashi R. | Amplification of target nucleotide sequence without polymerase chain reaction |
| JP2006500959A (ja) | 2002-09-30 | 2006-01-12 | パラレル バイオサイエンス， インコーポレイテッド | 動的サンプリング結合によるポリヌクレオチド合成および標識化 |
| WO2005047543A2 (en) | 2003-06-10 | 2005-05-26 | Applera Corporation | Ligation assay |
| EP1664265A4 (en) | 2003-07-21 | 2009-11-25 | Seng Entpr Ltd | IMPROVED MULTIPLAY PLATE |
| US7432055B2 (en) | 2004-03-05 | 2008-10-07 | Uchicago Argonne Llc | Dual phase multiplex polymerase chain reaction |
| EP3985125A1 (en) | 2004-06-01 | 2022-04-20 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
| CN101189345A (zh) | 2005-02-01 | 2008-05-28 | Ab先进基因分析公司 | 珠基测序的试剂、方法和文库 |
| US7604940B1 (en) | 2005-03-16 | 2009-10-20 | Applied Biosystems, Llc | Compositions and methods for analyzing isolated polynucleotides |
| US20060269934A1 (en) | 2005-03-16 | 2006-11-30 | Applera Corporation | Compositions and methods for clonal amplification and analysis of polynucleotides |
| GB0514936D0 (en) * | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| GB0514910D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Method for sequencing a polynucleotide template |
| EP1929049B1 (en) | 2005-07-25 | 2013-04-10 | Alere San Diego, Inc. | Methods for multiplexing recombinase polymerase amplification |
| EP1929045B9 (en) * | 2005-09-06 | 2012-06-20 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
| EP1987159B2 (en) | 2006-02-08 | 2020-08-12 | Illumina Cambridge Limited | Method for sequencing a polynucleotide template |
| EP2021503A1 (en) * | 2006-03-17 | 2009-02-11 | Solexa Ltd. | Isothermal methods for creating clonal single molecule arrays |
| US7282337B1 (en) | 2006-04-14 | 2007-10-16 | Helicos Biosciences Corporation | Methods for increasing accuracy of nucleic acid sequencing |
| JP2009538123A (ja) | 2006-04-19 | 2009-11-05 | アプライド バイオシステムズ， エルエルシー | ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー |
| JP2009535053A (ja) | 2006-05-04 | 2009-10-01 | エーエスエム サイエンティフィック， インコーポレイテッド | レコンビナーゼポリメラーゼ増幅 |
| EP2049682A2 (en) | 2006-07-31 | 2009-04-22 | Illumina Cambridge Limited | Method of library preparation avoiding the formation of adaptor dimers |
| US7754429B2 (en) | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
| US20080118917A1 (en) | 2006-11-21 | 2008-05-22 | Applera Corporation | Isothermal SNP Detection Method |
| EP2639578B1 (en) | 2006-12-14 | 2016-09-14 | Life Technologies Corporation | Apparatus for measuring analytes using large scale fet arrays |
| US8262900B2 (en) * | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US7932034B2 (en) | 2006-12-20 | 2011-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Heat and pH measurement for sequencing of DNA |
| JP5368321B2 (ja) | 2007-03-02 | 2013-12-18 | ディ・エヌ・エイ・エレクトロニクス・リミテッド | 固相ｐＨ検出を用いたｑＰＣＲ |
| US9029085B2 (en) | 2007-03-07 | 2015-05-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| US20090093378A1 (en) | 2007-08-29 | 2009-04-09 | Helen Bignell | Method for sequencing a polynucleotide template |
| US8003330B2 (en) | 2007-09-28 | 2011-08-23 | Pacific Biosciences Of California, Inc. | Error-free amplification of DNA for clonal sequencing |
| US20090286286A1 (en) | 2007-11-06 | 2009-11-19 | Ambergen , Inc. | Methods for controlling amplification |
| WO2009067632A1 (en) | 2007-11-20 | 2009-05-28 | Applied Biosystems Inc. | Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds |
| CN102027130A (zh) | 2008-02-05 | 2011-04-20 | 霍夫曼-拉罗奇有限公司 | 配对末端测序法 |
| WO2009102896A2 (en) * | 2008-02-12 | 2009-08-20 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
| US8034568B2 (en) * | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
| GB0810573D0 (en) | 2008-06-10 | 2008-07-16 | Univ Westminster | Anti-bacterial ligase inhibitors |
| CN102119225B (zh) * | 2008-06-11 | 2015-04-08 | 基因排列技术有限公司 | 等温核酸扩增 |
| WO2010003132A1 (en) | 2008-07-02 | 2010-01-07 | Illumina Cambridge Ltd. | Using populations of beads for the fabrication of arrays on surfaces |
| US8530156B2 (en) * | 2008-08-08 | 2013-09-10 | President And Fellows Of Harvard College | Chemically cleavable phosphoramidite linkers for sequencing by ligation |
| CN101413034B (zh) | 2008-11-21 | 2011-02-09 | 东南大学 | 高通量核酸分子克隆制备分子克隆芯片的方法 |
| EP2607496B1 (en) | 2008-12-23 | 2014-07-16 | Illumina, Inc. | Methods useful in nucleic acid sequencing protocols |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| WO2010111691A2 (en) * | 2009-03-27 | 2010-09-30 | Life Technologies Corp | Conjugates of biomolecules to nanoparticles |
| US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
| US8574835B2 (en) | 2009-05-29 | 2013-11-05 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
| EP3663750B1 (en) | 2009-05-29 | 2021-11-03 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
| EP3360974B1 (en) | 2009-06-05 | 2025-04-09 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification reagents |
| US8574864B2 (en) * | 2009-11-05 | 2013-11-05 | Epicentre Technologies Corporation | Methods and kits for 3'-end-tagging of RNA |
| EP2539464B1 (en) | 2010-02-23 | 2016-11-16 | Illumina, Inc. | Amplification methods to minimise sequence specific bias |
| US20110257385A1 (en) | 2010-02-23 | 2011-10-20 | Life Technologies Corporation | Methods for flip-strand immobilizing and sequencing nucleic acids |
| AU2011220536B2 (en) | 2010-02-26 | 2012-05-24 | Life Technologies Corporation | Modified proteins and methods of making and using same |
| US9029103B2 (en) | 2010-08-27 | 2015-05-12 | Illumina Cambridge Limited | Methods for sequencing polynucleotides |
| EP2426214A1 (en) | 2010-09-01 | 2012-03-07 | Koninklijke Philips Electronics N.V. | Method for amplifying nucleic acids |
| EP2617061B1 (en) | 2010-09-15 | 2021-06-30 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
| CN110079588B (zh) | 2010-12-17 | 2024-03-15 | 生命技术公司 | 用于核酸扩增的方法、组合物、系统、仪器和试剂盒 |
| EP3733867A1 (en) | 2011-01-17 | 2020-11-04 | Life Technologies Corporation | Enzymatic ligation of nucleic acids |
| US20130344540A1 (en) | 2011-02-03 | 2013-12-26 | Mark T. Reed | Methods for minimizing sequence specific bias |
| EP3978620A1 (en) | 2011-04-07 | 2022-04-06 | Abbott Diagnostics Scarborough, Inc. | Monitoring recombinase polymerase amplification mixtures |
| WO2013019361A1 (en) | 2011-07-07 | 2013-02-07 | Life Technologies Corporation | Sequencing methods |
| DE102011054101A1 (de) | 2011-09-30 | 2013-04-04 | Albert-Ludwigs-Universität Freiburg | Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen |
| EP3095879B1 (en) | 2012-04-19 | 2018-09-19 | Life Technologies Corporation | Nucleic acid amplification |
| CN104471075B (zh) | 2012-04-19 | 2018-06-22 | 生命技术公司 | 核酸扩增 |
| US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| EP2888371B1 (en) | 2012-08-24 | 2017-08-02 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid paired end sequencing |
| EP3257952B1 (en) | 2012-09-11 | 2020-02-12 | Life Technologies Corporation | Nucleic acid amplification |
| WO2014043143A1 (en) | 2012-09-11 | 2014-03-20 | Life Technologies Corporation | Nucleic acid amplification |
| EP3249057B1 (en) | 2013-03-14 | 2019-05-01 | Life Technologies Corporation | Matrix arrays and methods for making same |
-
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