JP5907990B2 - 核酸増幅のための方法、組成物、システム、装置およびキット - Google Patents

核酸増幅のための方法、組成物、システム、装置およびキット Download PDF

Info

Publication number
JP5907990B2
JP5907990B2 JP2013544833A JP2013544833A JP5907990B2 JP 5907990 B2 JP5907990 B2 JP 5907990B2 JP 2013544833 A JP2013544833 A JP 2013544833A JP 2013544833 A JP2013544833 A JP 2013544833A JP 5907990 B2 JP5907990 B2 JP 5907990B2
Authority
JP
Japan
Prior art keywords
primer
nucleic acid
strand
reverse
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2013544833A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014503207A5 (enExample
JP2014503207A (ja
Inventor
ビン リ
ビン リ
カイ シン ラオ
カイ シン ラオ
ジェニファー オニール
ジェニファー オニール
ジェニファー クンケル
ジェニファー クンケル
ケリー ハレイ
ケリー ハレイ
レイチェル カシンスカス
レイチェル カシンスカス
シャオチュン マ
シャオチュン マ
ピウス ブルゾスカ
ピウス ブルゾスカ
Original Assignee
ライフ テクノロジーズ コーポレーション
ライフ テクノロジーズ コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ライフ テクノロジーズ コーポレーション, ライフ テクノロジーズ コーポレーション filed Critical ライフ テクノロジーズ コーポレーション
Publication of JP2014503207A publication Critical patent/JP2014503207A/ja
Publication of JP2014503207A5 publication Critical patent/JP2014503207A5/ja
Application granted granted Critical
Publication of JP5907990B2 publication Critical patent/JP5907990B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C12Q1/46Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase involving cholinesterase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6846Common amplification features
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119Strand displacement amplification [SDA]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/537Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2013544833A 2010-12-17 2011-12-16 核酸増幅のための方法、組成物、システム、装置およびキット Active JP5907990B2 (ja)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US201061424599P 2010-12-17 2010-12-17
US61/424,599 2010-12-17
US201161445324P 2011-02-22 2011-02-22
US61/445,324 2011-02-22
US201161451919P 2011-03-11 2011-03-11
US61/451,919 2011-03-11
US201161526478P 2011-08-23 2011-08-23
US61/526,478 2011-08-23
US201161552660P 2011-10-28 2011-10-28
US61/552,660 2011-10-28
PCT/US2011/065535 WO2012083189A2 (en) 2010-12-17 2011-12-16 Methods, compositions, systems, apparatuses and kits for nucleic acid amplification

Publications (3)

Publication Number Publication Date
JP2014503207A JP2014503207A (ja) 2014-02-13
JP2014503207A5 JP2014503207A5 (enExample) 2014-11-06
JP5907990B2 true JP5907990B2 (ja) 2016-05-11

Family

ID=45478545

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013544833A Active JP5907990B2 (ja) 2010-12-17 2011-12-16 核酸増幅のための方法、組成物、システム、装置およびキット

Country Status (5)

Country Link
US (10) US20120156728A1 (enExample)
EP (3) EP2652148B1 (enExample)
JP (1) JP5907990B2 (enExample)
CN (3) CN110079588B (enExample)
WO (1) WO2012083189A2 (enExample)

Families Citing this family (148)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090047673A1 (en) 2006-08-22 2009-02-19 Cary Robert B Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
WO2009137059A1 (en) 2008-05-05 2009-11-12 Los Alamos National Security, Llc Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control
EP3216874A1 (en) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US10030045B2 (en) 2010-02-19 2018-07-24 Ohio State Innovation Foundation Primers and methods for nucleic acid amplification
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
KR20130113447A (ko) 2010-09-24 2013-10-15 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
GB2499340B (en) 2010-10-04 2015-10-28 Genapsys Inc Methods for sequencing nucleic acids
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
CN110079588B (zh) 2010-12-17 2024-03-15 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
WO2012099832A2 (en) 2011-01-17 2012-07-26 Life Technologies Corporation Enzymatic ligation of nucleic acids
WO2012099896A2 (en) 2011-01-17 2012-07-26 Life Technologies Corporation Workflow for detection of ligands using nucleic acids
BR112013020220B1 (pt) 2011-02-09 2020-03-17 Natera, Inc. Método para determinar o estado de ploidia de um cromossomo em um feto em gestação
CA2856304C (en) 2011-04-20 2017-05-16 Mesa Tech International, Inc. Oscillating amplification reaction for nucleic acids
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
WO2013082619A1 (en) 2011-12-01 2013-06-06 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
WO2013112923A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP3461910B1 (en) * 2012-04-19 2020-08-26 Life Technologies Corporation Nucleic acid amplification
SG11201406717RA (en) 2012-04-19 2014-11-27 Life Technologies Corp Nucleic acid amplification
KR20150011359A (ko) 2012-04-19 2015-01-30 라이프 테크놀로지스 코포레이션 디지털 pcr을 수행하는 방법
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
KR101890466B1 (ko) * 2012-07-24 2018-08-21 내테라, 인코포레이티드 고도의 다중 pcr 방법 및 조성물
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
WO2014031163A1 (en) * 2012-08-24 2014-02-27 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid paired end sequencing
WO2014043143A1 (en) * 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
EP2895620B1 (en) 2012-09-11 2017-08-02 Life Technologies Corporation Nucleic acid amplification
EP2964789A4 (en) * 2013-03-06 2016-11-02 Ohio State Innovation Foundation ISOTHERMIC NUCLEIC ACID AMPLIFICATION AND LIBRARY GENERATION AND CLONING GENERATION IN SEQUENCING
US10590474B2 (en) * 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
US20140255928A1 (en) * 2013-03-11 2014-09-11 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US10975423B2 (en) * 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
CN110066853B (zh) 2013-03-14 2023-03-28 生命技术公司 基质阵列和其制备方法
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
CA2896879C (en) 2013-03-15 2020-09-22 Genapsys, Inc. Systems and methods for biological analysis
US9409139B2 (en) 2013-08-05 2016-08-09 Twist Bioscience Corporation De novo synthesized gene libraries
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
EP3792921A1 (en) 2013-12-11 2021-03-17 Genapsys, Inc. Systems and methods for biological analysis and computation
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015161054A2 (en) 2014-04-18 2015-10-22 Genapsys, Inc. Methods and systems for nucleic acid amplification
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
EP3134541B1 (en) 2014-04-21 2020-08-19 Natera, Inc. Detecting copy number variations (cnv) of chromosomal segments in cancer
SG10202004286UA (en) 2014-05-08 2020-06-29 Fluidigm Corp Integrated single cell sequencing
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
EP3155127B1 (en) 2014-06-13 2020-07-22 Life Technologies Corporation Multiplex nucleic acid amplification
CN107002071A (zh) 2014-11-27 2017-08-01 株式会社日立高新技术 光点阵列基板、其制造方法、核酸聚合物解析方法以及装置
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA3253836A1 (en) 2015-02-04 2025-12-01 Twist Bioscience Corp Compositions and methods for synthetic gene assembly
AU2016220404B2 (en) * 2015-02-17 2021-05-27 Mgi Tech Co., Ltd. DNA sequencing using controlled strand displacement
GB201502645D0 (en) * 2015-02-17 2015-04-01 Touchlight Genetics Ltd Method
ES2924907T3 (es) 2015-03-05 2022-10-11 Life Technologies Corp Estabilización superficial de biosensores
EP4119677B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
JP6830443B2 (ja) 2015-04-24 2021-02-17 メサ バイオテック,インク. 流体検査用カセット
WO2016170179A1 (en) 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
WO2016170182A1 (en) 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
EP3289105B1 (en) 2015-04-29 2021-01-20 The Regents of The University of California Compositions and methods for constructing strand specific cdna libraries
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
CA2985545C (en) 2015-05-29 2021-02-09 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
US10344336B2 (en) 2015-06-09 2019-07-09 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
AU2016324296A1 (en) 2015-09-18 2018-04-12 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
US20180291413A1 (en) 2015-10-06 2018-10-11 Thermo Fisher Scientific Geneart Gmbh Devices and methods for producing nucleic acids and proteins
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
AU2017218431B2 (en) 2016-02-08 2022-08-04 RGENE, Inc. Multiple ligase compositions, systems, and methods
RU2760913C2 (ru) 2016-04-15 2021-12-01 Натера, Инк. Способы выявления рака легкого
JP2017209051A (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の検出方法、標的生体分子検出用基板、及び標的生体分子検出装置
FI3464628T3 (fi) 2016-06-06 2024-05-03 Redvault Biosciences Lp Kohdereportterikonstruktit ja niiden käyttö
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
CN109790575A (zh) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
EP4039824A1 (en) * 2016-08-15 2022-08-10 Pacific Biosciences of California, Inc. Method and system for sequencing nucleic acids
CA3034769A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
CN110248724B (zh) 2016-09-21 2022-11-18 特韦斯特生物科学公司 基于核酸的数据存储
JP7018940B2 (ja) * 2016-10-05 2022-02-14 エフ.ホフマン-ラ ロシュ アーゲー ナノトランジスタを使用した核酸配列決定
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
CN110366613A (zh) 2016-12-16 2019-10-22 特韦斯特生物科学公司 免疫突触的变体文库及其合成
CN114958998A (zh) * 2017-01-10 2022-08-30 深圳华大智造科技股份有限公司 一种提高核酸聚合测序质量的方法、反应体系和试剂盒
WO2018137826A1 (en) 2017-01-26 2018-08-02 Qiagen Gmbh Method for enriching template nucleic acids
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
EP3607087A4 (en) 2017-04-04 2020-12-30 Omniome, Inc. FLUIDIC APPARATUS AND USEFUL METHODS FOR CHEMICAL AND BIOLOGICAL REACTIONS
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
KR20200075814A (ko) 2017-08-15 2020-06-26 옴니옴 인코포레이티드 화학적 및 생물학적 분석물의 검출에 유용한 스캐닝 장치 및 방법
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
EP3684951A4 (en) 2017-09-21 2021-06-16 Genapsys, Inc. NUCLEIC ACID SEQUENCING SYSTEMS AND METHODS
US11414687B2 (en) * 2017-10-04 2022-08-16 Centrillion Technology Holdings Corporation Method and system for enzymatic synthesis of oligonucleotides
EP3697932A1 (en) 2017-10-19 2020-08-26 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
KR102637566B1 (ko) 2017-10-20 2024-02-16 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US12226745B2 (en) 2017-11-07 2025-02-18 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
EP4310196A3 (en) 2017-11-07 2024-04-24 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
CN112041438B (zh) 2018-01-04 2025-05-23 特韦斯特生物科学公司 基于dna的数字信息存储
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
GB201804585D0 (en) * 2018-03-22 2018-05-09 Dnae Diagnostics Ltd Methods for amplication of nucleic acids with Endonuclease-Mediated shifting equilibrium amplification (EM-SEq)
US12024738B2 (en) 2018-04-14 2024-07-02 Natera, Inc. Methods for cancer detection and monitoring
AU2019255987A1 (en) 2018-04-19 2020-12-10 Pacific Biosciences Of California, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
WO2020018824A1 (en) * 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
US12421628B2 (en) 2018-07-23 2025-09-23 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
CN113166805B (zh) 2018-12-04 2024-07-16 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序和其他分析测定的混合相流体
KR102872251B1 (ko) 2018-12-05 2025-10-16 일루미나 케임브리지 리미티드 브릿지 증폭에 의한 클러스터 생성을 위한 방법 및 조성물
US12385083B2 (en) 2018-12-10 2025-08-12 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
EP3899036A1 (en) * 2018-12-17 2021-10-27 Illumina Cambridge Limited Primer oligonucleotide for sequencing
WO2020126602A1 (en) 2018-12-18 2020-06-25 Illumina Cambridge Limited Methods and compositions for paired end sequencing using a single surface primer
EP3899032A2 (en) 2018-12-20 2021-10-27 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
CA3124980A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
KR20210138017A (ko) 2019-02-11 2021-11-18 울티마 제노믹스, 인크. 핵산 분석 방법
WO2020172444A1 (en) 2019-02-20 2020-08-27 Omniome, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
KR20210143766A (ko) 2019-02-26 2021-11-29 트위스트 바이오사이언스 코포레이션 Glp1 수용체에 대한 변이체 핵산 라이브러리
CN113785057A (zh) 2019-02-26 2021-12-10 特韦斯特生物科学公司 用于抗体优化的变异核酸文库
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
EP4403648B1 (en) 2019-05-03 2025-08-20 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
JP2022550497A (ja) 2019-06-21 2022-12-02 ツイスト バイオサイエンス コーポレーション バーコードに基づいた核酸配列アセンブリ
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
KR20220062302A (ko) 2019-08-21 2022-05-16 라이프 테크놀로지스 코포레이션 시퀀싱을 위한 시스템 및 방법
TW202124406A (zh) 2019-09-10 2021-07-01 美商歐姆尼歐美公司 核苷酸之可逆修飾
AU2020355027A1 (en) 2019-09-23 2022-04-21 Twist Bioscience Corporation Antibodies that bind CD3 Epsilon
CA3155629A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
US20230054204A1 (en) 2020-02-04 2023-02-23 Pacific Biosciences Of California, Inc. Flow cells and methods for their manufacture and use
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US20220170093A1 (en) 2020-11-16 2022-06-02 Life Technologies Corporation System and method for sequencing
MX2023007842A (es) 2021-01-08 2023-09-19 Cellanome Inc Dispositivos y metodos para analizar muestras biologicas.
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11236388B1 (en) 2021-06-17 2022-02-01 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
GB202215624D0 (en) 2022-10-21 2022-12-07 Dnae Diagnostics Ltd Clonal amplification

Family Cites Families (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1009396A (en) 1911-06-03 1911-11-21 Arthur E Fahrman Perforating-machine.
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5426180A (en) 1991-03-27 1995-06-20 Research Corporation Technologies, Inc. Methods of making single-stranded circular oligonucleotides
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
RU2048522C1 (ru) 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
US6001568A (en) 1992-10-26 1999-12-14 Institut Belka Solid medium for amplification and expression of nucleic acids as colonies
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
AU684279B2 (en) 1993-04-12 1997-12-11 Northwestern University Method of forming oligonucleotides
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5670325A (en) 1996-08-14 1997-09-23 Exact Laboratories, Inc. Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample
EP2368897B1 (en) 1996-02-09 2016-10-19 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US5928870A (en) 1997-06-16 1999-07-27 Exact Laboratories, Inc. Methods for the detection of loss of heterozygosity
WO1998008975A1 (en) 1996-08-29 1998-03-05 Daikin Industries, Ltd. Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
ATE364718T1 (de) 1997-04-01 2007-07-15 Solexa Ltd Verfahren zur vervielfältigung von nukleinsäure
WO1999019341A1 (en) 1997-10-10 1999-04-22 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) * 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
GB9902970D0 (en) 1999-02-11 1999-03-31 Zeneca Ltd Novel matrix
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
AUPQ008799A0 (en) * 1999-04-30 1999-05-27 Tillett, Daniel Genome sequencing
US6300070B1 (en) * 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
EP1313880A2 (en) 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
AR031640A1 (es) * 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
WO2002072772A2 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
DE60226856D1 (de) 2001-04-20 2008-07-10 Penn State Res Found Verfahren zur manipulation von nukleinsäuren
AU2002258997A1 (en) 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
AU2003215391B2 (en) 2002-02-21 2007-05-24 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20050118616A1 (en) * 2002-08-16 2005-06-02 Kawashima Tadashi R. Amplification of target nucleotide sequence without polymerase chain reaction
JP2006500959A (ja) 2002-09-30 2006-01-12 パラレル バイオサイエンス, インコーポレイテッド 動的サンプリング結合によるポリヌクレオチド合成および標識化
US7255994B2 (en) 2003-06-10 2007-08-14 Applera Corporation Ligation assay
EP1664265A4 (en) 2003-07-21 2009-11-25 Seng Entpr Ltd IMPROVED MULTIPLAY PLATE
US7432055B2 (en) 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
JP5026958B2 (ja) 2004-06-01 2012-09-19 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅
CN101189345A (zh) 2005-02-01 2008-05-28 Ab先进基因分析公司 珠基测序的试剂、方法和文库
WO2006099579A2 (en) * 2005-03-16 2006-09-21 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
US7604940B1 (en) 2005-03-16 2009-10-20 Applied Biosystems, Llc Compositions and methods for analyzing isolated polynucleotides
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514936D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
CA2616241C (en) 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
JP2009506788A (ja) * 2005-09-06 2009-02-19 ジェン−プローブ・インコーポレーテッド 核酸の等温増幅のための方法、組成物及びキット
CA2641851A1 (en) 2006-02-08 2007-08-16 Eric Hans Vermaas Method for sequencing a polynucleotide template
EP2021503A1 (en) * 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
US7282337B1 (en) 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
AU2007237909A1 (en) 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
CA2650993C (en) 2006-05-04 2015-06-16 Asm Scientific, Inc. Recombinase polymerase amplification
WO2008015396A2 (en) 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US20080118917A1 (en) 2006-11-21 2008-05-22 Applera Corporation Isothermal SNP Detection Method
US8262900B2 (en) * 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
AU2007334393A1 (en) 2006-12-14 2008-06-26 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
CN101743319B (zh) 2007-03-02 2013-03-06 Dna电子有限公司 使用固态pH传感器的qPCR
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
EP2191011B1 (en) 2007-08-29 2017-03-29 Illumina Cambridge Limited Method for sequencing a polynucleotide template
AU2008307617B2 (en) 2007-09-28 2013-05-23 Pacific Biosciences Of California, Inc. Error-free amplification of DNA for clonal sequencing
US20090286286A1 (en) 2007-11-06 2009-11-19 Ambergen , Inc. Methods for controlling amplification
US20090171078A1 (en) 2007-11-20 2009-07-02 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorotiolate compounds
EP2242855A1 (en) 2008-02-05 2010-10-27 Roche Diagnostics GmbH Paired end sequencing
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
EP2247727A4 (en) * 2008-02-12 2011-08-03 Nugen Technologies Inc PROCESS FOR ARCHIVING AND CLONING EXPANSION
GB0810573D0 (en) 2008-06-10 2008-07-16 Univ Westminster Anti-bacterial ligase inhibitors
CN102119225B (zh) * 2008-06-11 2015-04-08 基因排列技术有限公司 等温核酸扩增
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US8530156B2 (en) * 2008-08-08 2013-09-10 President And Fellows Of Harvard College Chemically cleavable phosphoramidite linkers for sequencing by ligation
CN101413034B (zh) 2008-11-21 2011-02-09 东南大学 高通量核酸分子克隆制备分子克隆芯片的方法
EP2607496B1 (en) 2008-12-23 2014-07-16 Illumina, Inc. Methods useful in nucleic acid sequencing protocols
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US20100261185A1 (en) * 2009-03-27 2010-10-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
EP2435461B1 (en) 2009-05-29 2017-08-09 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
US8574864B2 (en) * 2009-11-05 2013-11-05 Epicentre Technologies Corporation Methods and kits for 3'-end-tagging of RNA
US8759037B2 (en) 2010-02-23 2014-06-24 Illumina Cambridge Limited Amplification methods to minimise sequence specific bias
US20110257385A1 (en) 2010-02-23 2011-10-20 Life Technologies Corporation Methods for flip-strand immobilizing and sequencing nucleic acids
AU2011220536B2 (en) 2010-02-26 2012-05-24 Life Technologies Corporation Modified proteins and methods of making and using same
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
EP2426214A1 (en) 2010-09-01 2012-03-07 Koninklijke Philips Electronics N.V. Method for amplifying nucleic acids
US9618475B2 (en) 2010-09-15 2017-04-11 Life Technologies Corporation Methods and apparatus for measuring analytes
CN110079588B (zh) 2010-12-17 2024-03-15 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
WO2012099832A2 (en) 2011-01-17 2012-07-26 Life Technologies Corporation Enzymatic ligation of nucleic acids
WO2012106072A2 (en) 2011-02-03 2012-08-09 Illumina, Inc. Methods for minimizing sequence specific bias
JP5961247B2 (ja) 2011-04-07 2016-08-02 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅混合物のモニタリング
US9139874B2 (en) 2011-07-07 2015-09-22 Life Technologies Corporation Bi-directional sequencing compositions and methods
DE102011054101A1 (de) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen
SG11201406717RA (en) 2012-04-19 2014-11-27 Life Technologies Corp Nucleic acid amplification
EP3461910B1 (en) 2012-04-19 2020-08-26 Life Technologies Corporation Nucleic acid amplification
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014031163A1 (en) 2012-08-24 2014-02-27 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid paired end sequencing
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
EP2895620B1 (en) 2012-09-11 2017-08-02 Life Technologies Corporation Nucleic acid amplification
CN110066853B (zh) 2013-03-14 2023-03-28 生命技术公司 基质阵列和其制备方法

Also Published As

Publication number Publication date
US11578360B2 (en) 2023-02-14
US20230175051A1 (en) 2023-06-08
US20180010177A1 (en) 2018-01-11
US9765388B2 (en) 2017-09-19
US20160186224A1 (en) 2016-06-30
US10233488B2 (en) 2019-03-19
US10597705B2 (en) 2020-03-24
EP2652148B1 (en) 2016-11-30
EP3147374A1 (en) 2017-03-29
CN110079588A (zh) 2019-08-02
CN118086471A (zh) 2024-05-28
US20150031026A1 (en) 2015-01-29
US20170067098A1 (en) 2017-03-09
US20210032691A1 (en) 2021-02-04
US20190264272A1 (en) 2019-08-29
EP3147374B1 (en) 2019-01-30
US10913976B2 (en) 2021-02-09
CN103370425B (zh) 2019-03-19
WO2012083189A3 (en) 2012-08-23
US10093969B2 (en) 2018-10-09
WO2012083189A2 (en) 2012-06-21
US9476080B2 (en) 2016-10-25
CN110079588B (zh) 2024-03-15
JP2014503207A (ja) 2014-02-13
US12351865B2 (en) 2025-07-08
CN103370425A (zh) 2013-10-23
US20120156728A1 (en) 2012-06-21
US20190024159A1 (en) 2019-01-24
US20150275284A1 (en) 2015-10-01
US9315861B2 (en) 2016-04-19
EP3564392B1 (en) 2021-11-24
EP3564392A1 (en) 2019-11-06
EP2652148A2 (en) 2013-10-23

Similar Documents

Publication Publication Date Title
JP5907990B2 (ja) 核酸増幅のための方法、組成物、システム、装置およびキット
CN104781418B (zh) 用于核酸配对末端测序的方法、组合物、系统、仪器和试剂盒
US9309566B2 (en) Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
CN111094584B (zh) 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
WO2017214561A1 (en) Methods and compositions for nucleic acid amplification
JP2022546485A (ja) 腫瘍高精度アッセイのための組成物および方法
US20100151473A1 (en) Methods and compositions for hybridizing nucleic acids
HK40049316A (en) Compositions and methods for improving sample identification in indexed nucleic acid libraries
HK40049316B (en) Compositions and methods for improving sample identification in indexed nucleic acid libraries
HK40015459A (en) Compositions and methods for improving sample identification in indexed nucleic acid libraries
HK40015459B (en) Compositions and methods for improving sample identification in indexed nucleic acid libraries
HK1245843A1 (en) Dna sequencing using controlled strand displacement
HK1245843B (en) Dna sequencing using controlled strand displacement

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20131111

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140605

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140919

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20140919

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20150324

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150928

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20151228

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160126

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160222

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160322

R150 Certificate of patent or registration of utility model

Ref document number: 5907990

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250