ATE479780T1 - Qpcr unter verwendung eines ionen-sensitiven feldeffekt-transistors zur ph-messung - Google Patents

Qpcr unter verwendung eines ionen-sensitiven feldeffekt-transistors zur ph-messung

Info

Publication number
ATE479780T1
ATE479780T1 AT07726604T AT07726604T ATE479780T1 AT E479780 T1 ATE479780 T1 AT E479780T1 AT 07726604 T AT07726604 T AT 07726604T AT 07726604 T AT07726604 T AT 07726604T AT E479780 T1 ATE479780 T1 AT E479780T1
Authority
AT
Austria
Prior art keywords
field effect
effect transistor
sensitive field
qpcr
measurement
Prior art date
Application number
AT07726604T
Other languages
English (en)
Inventor
Christofer Toumazou
Sunil Purushothaman
Chung-Pei Ou
Original Assignee
Dna Electronics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna Electronics Ltd filed Critical Dna Electronics Ltd
Application granted granted Critical
Publication of ATE479780T1 publication Critical patent/ATE479780T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Insulated Gate Type Field-Effect Transistor (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
AT07726604T 2007-03-02 2007-03-02 Qpcr unter verwendung eines ionen-sensitiven feldeffekt-transistors zur ph-messung ATE479780T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2007/052010 WO2008107014A1 (en) 2007-03-02 2007-03-02 Qpcr using solid-state ph sensing

Publications (1)

Publication Number Publication Date
ATE479780T1 true ATE479780T1 (de) 2010-09-15

Family

ID=38353806

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07726604T ATE479780T1 (de) 2007-03-02 2007-03-02 Qpcr unter verwendung eines ionen-sensitiven feldeffekt-transistors zur ph-messung

Country Status (7)

Country Link
EP (1) EP2129792B1 (de)
JP (1) JP5368321B2 (de)
CN (1) CN101743319B (de)
AT (1) ATE479780T1 (de)
DE (1) DE602007008953D1 (de)
ES (1) ES2440572T3 (de)
WO (1) WO2008107014A1 (de)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2639578B1 (de) 2006-12-14 2016-09-14 Life Technologies Corporation Vorrichtung zur Messung von Analyten mithilfe großer FET-Arrays
EP2982437B1 (de) 2008-06-25 2017-12-06 Life Technologies Corporation Verfahren und vorrichtung zur messung von analyten mithilfe grossskaliger fet-arrays
CN103884760A (zh) * 2008-10-22 2014-06-25 生命技术公司 单芯片化学测定装置和单芯片核酸测定装置
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
GB201004147D0 (en) 2010-03-12 2010-04-28 Dna Electronics Ltd Method and apparatus for sensing methylation
CN119165030A (zh) 2010-06-30 2024-12-20 生命科技公司 阵列列积分器
WO2012003363A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
CN106932456B (zh) 2010-06-30 2020-02-21 生命科技公司 用于测试isfet阵列的方法和装置
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
US8653567B2 (en) 2010-07-03 2014-02-18 Life Technologies Corporation Chemically sensitive sensor with lightly doped drains
EP2617061B1 (de) 2010-09-15 2021-06-30 Life Technologies Corporation Verfahren und vorrichtung zur analytmessung
JP5920687B2 (ja) * 2010-09-17 2016-05-18 国立大学法人 東京大学 Ｄｎａ塩基配列解析装置およびｄｎａ塩基配列解析方法
CN103299182A (zh) 2010-09-24 2013-09-11 生命科技公司 匹配的晶体管对电路
JP2013543385A (ja) * 2010-10-08 2013-12-05 ディーエヌエー エレクトロニクス エルティーディー イオン性シグナルの増強
GB201018224D0 (en) * 2010-10-28 2010-12-15 Dna Electronics Chemical sensing device
US20120258546A1 (en) * 2011-04-08 2012-10-11 Life Technologies Corporation Automated On-Instrument pH Adjustment
GB201112140D0 (en) * 2011-07-14 2011-08-31 Dna Electronics Nucleic acid amplification
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
GB2500715A (en) * 2012-03-30 2013-10-02 Gene Onyx Ltd Single nucleotide polymorphism detection using an ISFET array
CN102631957B (zh) * 2012-04-13 2014-06-25 北京大学 带有栅压调制功能的超薄封装微流体系统及其制备方法
KR20150011359A (ko) 2012-04-19 2015-01-30 라이프 테크놀로지스 코포레이션 디지털 pcr을 수행하는 방법
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
GB201212775D0 (en) * 2012-07-18 2012-08-29 Dna Electronics Ltd Sensing apparatus and method
GB2509481A (en) * 2012-08-29 2014-07-09 Dna Electronics Ltd Ionic signal enhancement in nucleic acid synthesis reactions
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
CN104884948B (zh) * 2013-01-17 2018-01-09 株式会社日立高新技术 生物分子测量装置
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
WO2014149778A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensors with consistent sensor surface areas
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
US20140264471A1 (en) 2013-03-15 2014-09-18 Life Technologies Corporation Chemical device with thin conductive element
EP2972280B1 (de) 2013-03-15 2021-09-29 Life Technologies Corporation Chemischer sensor mit stetigen sensoroberflächen
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
US20160231251A1 (en) * 2013-09-04 2016-08-11 Credo Biomedical Pte Ltd. Assay test device, kit and method of using
US9310332B2 (en) * 2013-11-14 2016-04-12 Taiwan Semiconductor Manufacturing Company Limited Semiconductor device and selective heating thereof
CN103923998A (zh) * 2014-04-23 2014-07-16 浙江大学 一种应用于核酸扩增实时电化学pH检测的反应体系和检测方法
ES2887222T3 (es) 2014-04-24 2021-12-22 Lucira Health Inc Detección colorimétrica de amplificación de ácido nucleico
AU2015289828B2 (en) * 2014-07-15 2019-12-19 Illumina, Inc. Biochemically activated electronic device
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
KR102593647B1 (ko) 2014-12-18 2023-10-26 라이프 테크놀로지스 코포레이션 트랜스미터 구성을 갖춘 높은 데이터율 집적 회로
TW202024628A (zh) 2014-12-18 2020-07-01 美商生命技術公司 用於使用大規模fet陣列量測分析物之方法及設備
CN108291251A (zh) * 2015-09-22 2018-07-17 生命技术公司 用于核酸分析的系统和方法
EP3957398A1 (de) * 2015-09-29 2022-02-23 Life Technologies Corporation Systeme und verfahren zur ausführung von digitaler pcr
PT3430378T (pt) 2016-03-14 2022-09-15 Lucira Health Inc Dispositivos e métodos para modificar propriedades ópticas
CA3015376C (en) 2016-03-14 2023-11-14 Diassess Inc. Devices and methods for biological assay sample preparation and delivery
DK3429543T3 (da) 2016-03-14 2025-01-27 Pfizer Selektivt ventilerede biologiske analyseanordninger og tilhørende fremgangsmåder
CA3240706A1 (en) 2016-03-14 2017-09-21 Pfizer Inc. Systems and methods for performing biological assays
JP6239665B2 (ja) * 2016-03-16 2017-11-29 株式会社東芝 半導体装置
JPWO2017204294A1 (ja) * 2016-05-25 2019-02-21 株式会社ニコン 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置
JP2017209051A (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の検出方法、標的生体分子検出用基板、及び標的生体分子検出装置
JP2017209048A (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の濃度算出方法、標的生体分子濃度算出用ビーズ、ビーズのセット、及び標的生体分子濃度算出装置
JP2017209049A (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置
US11080848B2 (en) 2017-04-06 2021-08-03 Lucira Health, Inc. Image-based disease diagnostics using a mobile device
CN107460118B (zh) * 2017-07-24 2021-02-23 成都奇璞生物科技有限公司 一种基于场效应管的聚合酶链式反应的装置及其使用方法
US10549275B2 (en) 2017-09-14 2020-02-04 Lucira Health, Inc. Multiplexed biological assay device with electronic readout
CA3073868A1 (en) 2017-09-14 2019-03-21 Lucira Health, Inc. Multiplexed biological assay device with electronic readout
EP3717909A4 (de) * 2017-11-28 2021-12-29 Inanobio, Inc. Assays zur detektion eines feldeffekttransistorsensors und systeme und verfahren zur herstellung und verwendung davon
GB2570157A (en) * 2018-01-15 2019-07-17 Dnanudge Ltd Molecule detection
US10467679B1 (en) 2019-04-15 2019-11-05 Dnanudge Limited Product recommendation device and method
USD910200S1 (en) 2018-12-21 2021-02-09 Lucira Health, Inc. Test tube
US10811140B2 (en) 2019-03-19 2020-10-20 Dnanudge Limited Secure set-up of genetic related user account
US10699806B1 (en) 2019-04-15 2020-06-30 Dnanudge Limited Monitoring system, wearable monitoring device and method
USD953561S1 (en) 2020-05-05 2022-05-31 Lucira Health, Inc. Diagnostic device with LED display
USD962470S1 (en) 2020-06-03 2022-08-30 Lucira Health, Inc. Assay device with LCD display
CN113333042B (zh) * 2021-06-21 2022-04-22 太原理工大学 一种用于核酸检测的微流控芯片及其制作方法
US20240392360A1 (en) 2021-09-23 2024-11-28 miDiagnostics NV Nucleic acid detection
CN113789375B (zh) * 2021-10-14 2024-05-24 联合基因生物科技(上海)有限公司 一种基于硅基微流片的cyp2c19基因分型的检测试剂、试剂盒和方法
CN116694452B (zh) * 2023-07-24 2024-07-02 广东凯普科技智造有限公司 一种核酸pcr扩增杂交反应仪及扩增杂交一体机

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0105831D0 (en) * 2001-03-09 2001-04-25 Toumaz Technology Ltd Method for dna sequencing utilising enzyme linked field effect transistors
WO2002086162A1 (en) * 2001-04-23 2002-10-31 Samsung Electronics Co., Ltd. Molecular detection chip including mosfet, molecular detection device employing the chip, and molecular detection method using the device
US20050170347A1 (en) * 2001-12-19 2005-08-04 Yuji Miyahara Potentiometric dna microarray, process for producing the same and method of analyzing nucleic acid
US20050106587A1 (en) * 2001-12-21 2005-05-19 Micronas Gmbh Method for determining of nucleic acid analytes
CA2475348A1 (en) * 2002-02-12 2003-08-21 Cellectricon Ab Systems and methods for rapidly changing the solution environment around sensors
JP2003322630A (ja) * 2002-05-01 2003-11-14 Seiko Epson Corp バイオセンサ、バイオセンシングシステム、及びバイオセンシング方法
JP3903183B2 (ja) * 2004-02-03 2007-04-11 独立行政法人物質・材料研究機構 遺伝子検出電界効果デバイスおよびこれを用いた遺伝子多型解析方法
WO2006022370A1 (ja) * 2004-08-27 2006-03-02 National Institute For Materials Science 電界効果デバイスを用いたdna塩基配列解析方法及び塩基配列解析装置

Also Published As

Publication number Publication date
EP2129792A1 (de) 2009-12-09
JP5368321B2 (ja) 2013-12-18
JP2010519914A (ja) 2010-06-10
CN101743319A (zh) 2010-06-16
DE602007008953D1 (de) 2010-10-14
WO2008107014A1 (en) 2008-09-12
CN101743319B (zh) 2013-03-06
ES2440572T3 (es) 2014-01-29
EP2129792B1 (de) 2010-09-01

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