WO2009051214A1 - 核酸増幅法、それに用いる試薬及び試薬キット - Google Patents

核酸増幅法、それに用いる試薬及び試薬キット Download PDF

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Publication number
WO2009051214A1
WO2009051214A1 PCT/JP2008/068829 JP2008068829W WO2009051214A1 WO 2009051214 A1 WO2009051214 A1 WO 2009051214A1 JP 2008068829 W JP2008068829 W JP 2008068829W WO 2009051214 A1 WO2009051214 A1 WO 2009051214A1
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
reagent
internal standard
target nucleic
standard substance
Prior art date
Application number
PCT/JP2008/068829
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English (en)
French (fr)
Inventor
Toshihiro Yonekawa
Tsugunori Notomi
Hidetoshi Kanda
Norimitsu Hosaka
Original Assignee
Eiken Kagaku Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eiken Kagaku Kabushiki Kaisha filed Critical Eiken Kagaku Kabushiki Kaisha
Priority to ES08839845T priority Critical patent/ES2433667T3/es
Priority to CN2008801221502A priority patent/CN101903520B/zh
Priority to US12/738,413 priority patent/US8557523B2/en
Priority to JP2009538159A priority patent/JP5313157B2/ja
Priority to EP08839845.8A priority patent/EP2218780B1/en
Publication of WO2009051214A1 publication Critical patent/WO2009051214A1/ja
Priority to HK11100161.0A priority patent/HK1146086A1/xx
Priority to US13/929,703 priority patent/US20140308663A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　本発明の課題は、内部標準物質を使用する核酸の検出系において、標的核酸の精確な測定値を確保しつつ、安定した内部標準物質の増幅を行える方法及びそれに用いる試薬キットを提供することである。本発明は、内部標準物質を使用する試料中の標的核酸の増幅法において、内部標準物質を標的核酸よりも先行して増幅させることによって内部標準の増幅産物による標的核酸の増幅反応への影響を防ぐことを特徴とする核酸増幅法、それに用いる試薬並びに試薬キットに関する。
PCT/JP2008/068829 2007-10-19 2008-10-17 核酸増幅法、それに用いる試薬及び試薬キット WO2009051214A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
ES08839845T ES2433667T3 (es) 2007-10-19 2008-10-17 Método de amplificación de ácidos nucleicos, y reactivo y kit de reactivos para la utilización en el método
CN2008801221502A CN101903520B (zh) 2007-10-19 2008-10-17 核酸扩增方法以及在该方法中使用的试剂和试剂套件
US12/738,413 US8557523B2 (en) 2007-10-19 2008-10-17 Method of nucleic acid amplification and measuring reagent and reagent kit therefor
JP2009538159A JP5313157B2 (ja) 2007-10-19 2008-10-17 核酸増幅法、それに用いる試薬及び試薬キット
EP08839845.8A EP2218780B1 (en) 2007-10-19 2008-10-17 Nucleic acid amplification method, and reagent and reagent kit for use in the method
HK11100161.0A HK1146086A1 (en) 2007-10-19 2011-01-10 Nucleic acid amplification method, and reagent and reagent kit for use in the method
US13/929,703 US20140308663A1 (en) 2007-10-19 2013-06-27 Method of nucleic acid amplification and measuring reagent and reagent kit therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-271902 2007-10-19
JP2007271902 2007-10-19

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/738,413 A-371-Of-International US8557523B2 (en) 2007-10-19 2008-10-17 Method of nucleic acid amplification and measuring reagent and reagent kit therefor
US13/929,703 Division US20140308663A1 (en) 2007-10-19 2013-06-27 Method of nucleic acid amplification and measuring reagent and reagent kit therefor

Publications (1)

Publication Number Publication Date
WO2009051214A1 true WO2009051214A1 (ja) 2009-04-23

Family

ID=40567475

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/068829 WO2009051214A1 (ja) 2007-10-19 2008-10-17 核酸増幅法、それに用いる試薬及び試薬キット

Country Status (7)

Country Link
US (2) US8557523B2 (ja)
EP (1) EP2218780B1 (ja)
JP (2) JP5313157B2 (ja)
CN (1) CN101903520B (ja)
ES (1) ES2433667T3 (ja)
HK (1) HK1146086A1 (ja)
WO (1) WO2009051214A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013065574A1 (ja) 2011-10-31 2013-05-10 栄研化学株式会社 標的核酸の検出法
CN105132586A (zh) * 2015-09-10 2015-12-09 北京出入境检验检疫局检验检疫技术中心 含猪圆环病毒2型orf2基因的重组腺病毒在核酸扩增检测中作为标准样品的应用
JP2018086016A (ja) * 2014-05-12 2018-06-07 三菱ケミカル株式会社 菌叢解析方法と菌叢解析用デバイス

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GB0812862D0 (en) * 2008-07-14 2008-08-20 Lumora Ltd Nucleic acid amplification
CN102559861B (zh) * 2010-12-30 2016-08-10 上海星耀医学科技发展有限公司 沙眼衣原体核酸快速检测试剂盒
CN103320435B (zh) * 2013-06-28 2015-04-22 华南理工大学 含内标的单核细胞增生李斯特氏菌lamp检测试剂盒
CN103320434B (zh) * 2013-06-28 2015-04-22 华南理工大学 一种沙门氏菌lamp引物组和试剂盒及检测方法
GB201319180D0 (en) * 2013-10-30 2013-12-11 Mast Group Ltd Nucleic acid probe
KR20180040511A (ko) 2014-11-05 2018-04-20 캘리포니아 인스티튜트 오브 테크놀로지 유기체의 약물에 대한 반응의 미세유체 측정
WO2018058078A1 (en) 2016-09-23 2018-03-29 California Institute Of Technology Digital quantification of dna replication and/or chromosome segregation based determination of antimicrobial susceptibility
WO2018089943A1 (en) * 2016-11-10 2018-05-17 Slipchip Corporation Probe detection of loop-mediated amplification products
CN107338289A (zh) * 2017-06-20 2017-11-10 复旦大学附属华山医院 一种锁核酸修饰的lamp引物组合物及其应用
EP3695009A4 (en) 2017-10-11 2021-08-11 California Institute of Technology ANTIBIOTIC SENSITIVITY OF MICROORGANISMS AND RELATED COMPOSITIONS, PROCEDURES AND SYSTEMS
US10450616B1 (en) 2018-05-09 2019-10-22 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Chlamydia trachomatis
US10954572B2 (en) 2019-07-25 2021-03-23 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Neisseria gonorrhoeae
US11891662B2 (en) 2019-12-02 2024-02-06 Talis Biomedical Corporation Polynucleotides for amplification and detection of human beta actin
US11788129B2 (en) 2021-02-23 2023-10-17 Industrial Technology Research Institute Nucleic acid detection kit and nucleic acid detection method

Citations (4)

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JP3437816B2 (ja) 1999-04-20 2003-08-18 環境エンジニアリング株式会社 核酸の測定方法
WO2003089669A1 (fr) * 2002-04-22 2003-10-30 Kankyo Engineering Co., Ltd. Nouveau procede servant a mesurer un acide nucleique
JP3985959B2 (ja) 1999-04-20 2007-10-03 独立行政法人産業技術総合研究所 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法
JP2007271902A (ja) 2006-03-31 2007-10-18 Daiichikosho Co Ltd カラオケボックス

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AU2003211947A1 (en) * 2002-02-20 2003-09-09 Sysmex Corporation PRIMERS FOR NUCLEIC ACID AMPLIFICATION IN DETECTING HOUSEKEEPING GENE mRNA AND TEST METHOD USING THESE PRIMERS
JP2008515393A (ja) * 2004-09-20 2008-05-15 ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション 複数モードの多重化反応消去方法
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CN100441698C (zh) * 2005-03-23 2008-12-10 上海市血液中心 基于环介导的等温扩增技术的血液病毒核酸筛查方法
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JP3985959B2 (ja) 1999-04-20 2007-10-03 独立行政法人産業技術総合研究所 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法
WO2003089669A1 (fr) * 2002-04-22 2003-10-30 Kankyo Engineering Co., Ltd. Nouveau procede servant a mesurer un acide nucleique
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See also references of EP2218780A4
TANI H. ET AL.: "Technique for quantitative detection of specific DNA sequences using alternately binding quenching probe competitive assay combined with loop-mediated isothermal amplification", ANAL. CHEM., vol. 79, no. 15, 1 August 2007 (2007-08-01), pages 5608 - 5613, XP008109197 *
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013065574A1 (ja) 2011-10-31 2013-05-10 栄研化学株式会社 標的核酸の検出法
KR20140091562A (ko) 2011-10-31 2014-07-21 에이껜 가가꾸 가부시끼가이샤 표적 핵산의 검출법
US10876160B2 (en) 2011-10-31 2020-12-29 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid
JP2018086016A (ja) * 2014-05-12 2018-06-07 三菱ケミカル株式会社 菌叢解析方法と菌叢解析用デバイス
CN105132586A (zh) * 2015-09-10 2015-12-09 北京出入境检验检疫局检验检疫技术中心 含猪圆环病毒2型orf2基因的重组腺病毒在核酸扩增检测中作为标准样品的应用

Also Published As

Publication number Publication date
CN101903520B (zh) 2013-11-13
US20140308663A1 (en) 2014-10-16
JPWO2009051214A1 (ja) 2011-03-03
EP2218780B1 (en) 2013-07-24
ES2433667T3 (es) 2013-12-12
US20100233715A1 (en) 2010-09-16
EP2218780A1 (en) 2010-08-18
JP5313157B2 (ja) 2013-10-09
HK1146086A1 (en) 2011-05-13
CN101903520A (zh) 2010-12-01
EP2218780A4 (en) 2010-11-24
US8557523B2 (en) 2013-10-15
JP2013135696A (ja) 2013-07-11

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