JP2006519621A5 - - Google Patents
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- JP2006519621A5 JP2006519621A5 JP2006509236A JP2006509236A JP2006519621A5 JP 2006519621 A5 JP2006519621 A5 JP 2006519621A5 JP 2006509236 A JP2006509236 A JP 2006509236A JP 2006509236 A JP2006509236 A JP 2006509236A JP 2006519621 A5 JP2006519621 A5 JP 2006519621A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- nucleic acid
- polymerase
- molecule
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims 269
- 108020004414 DNA Proteins 0.000 claims 134
- 150000007523 nucleic acids Chemical class 0.000 claims 96
- 239000000203 mixture Substances 0.000 claims 80
- 102000039446 nucleic acids Human genes 0.000 claims 74
- 108020004707 nucleic acids Proteins 0.000 claims 74
- 102000053602 DNA Human genes 0.000 claims 50
- 230000000295 complement effect Effects 0.000 claims 48
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 35
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims 30
- 102100034343 Integrase Human genes 0.000 claims 26
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 26
- 239000003795 chemical substances by application Substances 0.000 claims 26
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 24
- 102000040430 polynucleotide Human genes 0.000 claims 20
- 108091033319 polynucleotide Proteins 0.000 claims 20
- 239000002157 polynucleotide Substances 0.000 claims 20
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 18
- 229920001519 homopolymer Polymers 0.000 claims 17
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 16
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 16
- 230000003321 amplification Effects 0.000 claims 15
- 238000003199 nucleic acid amplification method Methods 0.000 claims 15
- 238000006116 polymerization reaction Methods 0.000 claims 15
- 108010077544 Chromatin Proteins 0.000 claims 14
- 210000003483 chromatin Anatomy 0.000 claims 14
- 150000001875 compounds Chemical class 0.000 claims 14
- 241000713869 Moloney murine leukemia virus Species 0.000 claims 12
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 12
- 238000001514 detection method Methods 0.000 claims 12
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 12
- 238000012544 monitoring process Methods 0.000 claims 12
- 125000003729 nucleotide group Chemical group 0.000 claims 12
- 238000003752 polymerase chain reaction Methods 0.000 claims 12
- 238000006243 chemical reaction Methods 0.000 claims 10
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 10
- 239000002773 nucleotide Substances 0.000 claims 10
- -1 9 ° Nm polymerase Proteins 0.000 claims 9
- 238000012163 sequencing technique Methods 0.000 claims 9
- 108091034117 Oligonucleotide Proteins 0.000 claims 8
- 239000012634 fragment Substances 0.000 claims 8
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims 7
- 108010017826 DNA Polymerase I Proteins 0.000 claims 7
- 102000004594 DNA Polymerase I Human genes 0.000 claims 7
- 108010078851 HIV Reverse Transcriptase Proteins 0.000 claims 7
- 108060004795 Methyltransferase Proteins 0.000 claims 7
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims 7
- 206010036790 Productive cough Diseases 0.000 claims 7
- 229960003237 betaine Drugs 0.000 claims 7
- 230000027455 binding Effects 0.000 claims 7
- 210000004027 cell Anatomy 0.000 claims 7
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims 7
- 230000000694 effects Effects 0.000 claims 7
- 230000002708 enhancing effect Effects 0.000 claims 7
- 210000003780 hair follicle Anatomy 0.000 claims 7
- 238000010438 heat treatment Methods 0.000 claims 7
- 210000002445 nipple Anatomy 0.000 claims 7
- 210000003296 saliva Anatomy 0.000 claims 7
- 210000000582 semen Anatomy 0.000 claims 7
- 210000003802 sputum Anatomy 0.000 claims 7
- 208000024794 sputum Diseases 0.000 claims 7
- 210000004243 sweat Anatomy 0.000 claims 7
- 210000001519 tissue Anatomy 0.000 claims 7
- 210000002700 urine Anatomy 0.000 claims 7
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 claims 6
- 206010028980 Neoplasm Diseases 0.000 claims 6
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 claims 6
- 210000004369 blood Anatomy 0.000 claims 6
- 239000008280 blood Substances 0.000 claims 6
- 201000011510 cancer Diseases 0.000 claims 6
- 201000010099 disease Diseases 0.000 claims 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 6
- 238000009396 hybridization Methods 0.000 claims 6
- 238000010348 incorporation Methods 0.000 claims 6
- 239000000463 material Substances 0.000 claims 6
- 108020004999 messenger RNA Proteins 0.000 claims 6
- 210000002381 plasma Anatomy 0.000 claims 6
- 210000002966 serum Anatomy 0.000 claims 6
- 229940104230 thymidine Drugs 0.000 claims 6
- 102000023732 binding proteins Human genes 0.000 claims 5
- 108091008324 binding proteins Proteins 0.000 claims 5
- 229940104302 cytosine Drugs 0.000 claims 5
- 230000001404 mediated effect Effects 0.000 claims 5
- 230000009149 molecular binding Effects 0.000 claims 5
- 230000003362 replicative effect Effects 0.000 claims 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 4
- 229930024421 Adenine Natural products 0.000 claims 4
- 108060002716 Exonuclease Proteins 0.000 claims 4
- 108010001244 Tli polymerase Proteins 0.000 claims 4
- 229960000643 adenine Drugs 0.000 claims 4
- 102000013165 exonuclease Human genes 0.000 claims 4
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims 3
- 101710116602 DNA-Binding protein G5P Proteins 0.000 claims 3
- 101710162453 Replication factor A Proteins 0.000 claims 3
- 101710176758 Replication protein A 70 kDa DNA-binding subunit Proteins 0.000 claims 3
- 101710176276 SSB protein Proteins 0.000 claims 3
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 claims 3
- 239000002299 complementary DNA Substances 0.000 claims 3
- 230000014509 gene expression Effects 0.000 claims 3
- 230000003100 immobilizing effect Effects 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 239000000758 substrate Substances 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 2
- 108010010677 Phosphodiesterase I Proteins 0.000 claims 2
- 241000700605 Viruses Species 0.000 claims 2
- 239000000654 additive Substances 0.000 claims 2
- 230000000996 additive effect Effects 0.000 claims 2
- 238000001574 biopsy Methods 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 238000006073 displacement reaction Methods 0.000 claims 2
- 230000037452 priming Effects 0.000 claims 2
- 101710159080 Aconitate hydratase A Proteins 0.000 claims 1
- 101710159078 Aconitate hydratase B Proteins 0.000 claims 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims 1
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000044126 RNA-Binding Proteins Human genes 0.000 claims 1
- 101710105008 RNA-binding protein Proteins 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 102000004357 Transferases Human genes 0.000 claims 1
- 108090000992 Transferases Proteins 0.000 claims 1
- 125000003277 amino group Chemical group 0.000 claims 1
- 210000004102 animal cell Anatomy 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims 1
- 238000010367 cloning Methods 0.000 claims 1
- 239000000356 contaminant Substances 0.000 claims 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims 1
- 230000002950 deficient Effects 0.000 claims 1
- 238000004925 denaturation Methods 0.000 claims 1
- 230000036425 denaturation Effects 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 238000012268 genome sequencing Methods 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 238000002493 microarray Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 claims 1
- 230000002285 radioactive effect Effects 0.000 claims 1
- 238000010839 reverse transcription Methods 0.000 claims 1
- 102000033955 single-stranded RNA binding proteins Human genes 0.000 claims 1
- 108091000371 single-stranded RNA binding proteins Proteins 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US45306003P | 2003-03-07 | 2003-03-07 | |
| US60/453,060 | 2003-03-07 | ||
| PCT/US2004/006983 WO2004081225A2 (en) | 2003-03-07 | 2004-03-08 | Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006519621A JP2006519621A (ja) | 2006-08-31 |
| JP2006519621A5 true JP2006519621A5 (enExample) | 2007-04-05 |
| JP4773338B2 JP4773338B2 (ja) | 2011-09-14 |
Family
ID=32990716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006509236A Expired - Lifetime JP4773338B2 (ja) | 2003-03-07 | 2004-03-08 | Ｄｎａ重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US7718403B2 (enExample) |
| EP (2) | EP2374900B1 (enExample) |
| JP (1) | JP4773338B2 (enExample) |
| AT (1) | ATE484598T1 (enExample) |
| DE (1) | DE602004029560D1 (enExample) |
| DK (2) | DK2374900T3 (enExample) |
| WO (1) | WO2004081225A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10961562B2 (en) | 2016-09-06 | 2021-03-30 | Swift Biosciences, Inc. | Normalization of NGS library concentration |
Families Citing this family (171)
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| US8206913B1 (en) * | 2003-03-07 | 2012-06-26 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
| US20050181394A1 (en) * | 2003-06-20 | 2005-08-18 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| JP2007525963A (ja) | 2003-06-20 | 2007-09-13 | イルミナ インコーポレイテッド | 全ゲノム増幅および遺伝型決定のための方法および組成物 |
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| JP3972106B2 (ja) * | 2004-03-03 | 2007-09-05 | 大学共同利用機関法人情報・システム研究機構 | ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー |
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- 2004-03-08 US US10/795,667 patent/US7718403B2/en active Active
- 2004-03-08 EP EP04718499A patent/EP1604040B1/en not_active Expired - Lifetime
- 2004-03-08 JP JP2006509236A patent/JP4773338B2/ja not_active Expired - Lifetime
- 2004-03-08 AT AT04718499T patent/ATE484598T1/de not_active IP Right Cessation
-
2006
- 2006-11-06 US US11/593,625 patent/US20070054311A1/en not_active Abandoned
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10961562B2 (en) | 2016-09-06 | 2021-03-30 | Swift Biosciences, Inc. | Normalization of NGS library concentration |
| US12371731B2 (en) | 2016-09-06 | 2025-07-29 | Integrated Dna Technologies, Inc. | Normalization of NGS library concentration |
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