JP4773338B2 - Ｄｎａ重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析 - Google Patents
Ｄｎａ重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析 Download PDFInfo
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- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
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| US20040024193A1 (en) * | 2002-07-26 | 2004-02-05 | Williams Gregg T. | Method of detecting and quantifying hepatitis C virus |
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| EP1636337A4 (en) | 2003-06-20 | 2007-07-04 | Illumina Inc | METHODS AND COMPOSITIONS USEFUL FOR THE AMPLIFICATION AND GENOTYPING OF THE GENOME |
| US7459274B2 (en) * | 2004-03-02 | 2008-12-02 | Orion Genomics Llc | Differential enzymatic fragmentation by whole genome amplification |
| JP3972106B2 (ja) * | 2004-03-03 | 2007-09-05 | 大学共同利用機関法人情報・システム研究機構 | ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー |
| US8440404B2 (en) * | 2004-03-08 | 2013-05-14 | Rubicon Genomics | Methods and compositions for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation |
| PT1794327T (pt) | 2004-09-14 | 2017-01-24 | Argos Therapeutics Inc | Amplificação independente de estirpes de patogénios e vacinas para os mesmos |
| EP1848819A4 (en) * | 2005-02-16 | 2010-01-06 | Genetic Technologies Ltd | GENETIC ANALYSIS METHODS COMPRISING THE AMPLIFICATION OF COMPLEMENTARY DUPLICONS |
| US7449297B2 (en) | 2005-04-14 | 2008-11-11 | Euclid Diagnostics Llc | Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays |
| EP2620510B2 (en) | 2005-06-15 | 2020-02-19 | Complete Genomics Inc. | Single molecule arrays for genetic and chemical analysis |
| US20070003938A1 (en) * | 2005-06-30 | 2007-01-04 | Perlegen Sciences, Inc. | Hybridization of genomic nucleic acid without complexity reduction |
| WO2007018602A1 (en) | 2005-08-02 | 2007-02-15 | Rubicon Genomics, Inc. | Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method |
| US20070031857A1 (en) | 2005-08-02 | 2007-02-08 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction |
| US20090137415A1 (en) * | 2005-08-05 | 2009-05-28 | Euclid Diagnostics Llc | SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA) |
| CN101263227A (zh) * | 2005-09-16 | 2008-09-10 | 454生命科学公司 | cDNA文库制备 |
| US9134237B2 (en) * | 2005-09-20 | 2015-09-15 | Janssen Diagnotics, LLC | High sensitivity multiparameter method for rare event analysis in a biological sample |
| JP5457673B2 (ja) * | 2005-09-20 | 2014-04-02 | ベリデックス・リミテッド・ライアビリティ・カンパニー | ユニーク配列のｄｎａプローブを作製するための方法および組成物、ｄｎａプローブの標識、ならびにこれらプローブの使用 |
| US20070092899A1 (en) * | 2005-10-21 | 2007-04-26 | The Regents Of The University Of California | Multiple displacement amplification with blocker DNA |
| JP2009514555A (ja) | 2005-11-08 | 2009-04-09 | ユークリッド ダイアグノスティックス リミテッド ライアビリティー カンパニー | 癌評価の遺伝子関連ＣｐＧアイランドのメチル化アッセイのための材料と方法 |
| US20080057499A1 (en) * | 2006-02-06 | 2008-03-06 | Affymetrix, Inc. | Methods for high specificity whole genome amplification and hybridization |
| DE602007003331D1 (de) * | 2006-04-12 | 2009-12-31 | Medical Res Council | Verfahren zur bestimmung der kopienummer |
| WO2007136874A2 (en) * | 2006-05-18 | 2007-11-29 | President And Fellows Of Harvard College | Genomic library construction |
| EP2061801A4 (en) * | 2006-06-14 | 2009-11-11 | Living Microsystems Inc | DIAGNOSIS OF FETAL ANOMALIES BY COMPARATIVE GENOMIC HYBRIDIZATION ANALYSIS |
| EP2765202B1 (en) | 2006-08-01 | 2017-02-01 | Gen-Probe Incorporated | Methods of nonspecific target capture of nucleic acids |
| JP5420412B2 (ja) * | 2006-09-14 | 2014-02-19 | アイビス バイオサイエンシズ インコーポレイティッド | 病原体の同定のための標的全ゲノム増幅方法 |
| CN103952470B (zh) * | 2007-03-28 | 2017-11-10 | 信号诊断公司 | 高解析度分析核酸以检测序列变异的系统和方法 |
| US7794985B2 (en) * | 2007-04-04 | 2010-09-14 | Ghc Technologies, Inc. | Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins |
| CN101720359A (zh) * | 2007-06-01 | 2010-06-02 | 454生命科学公司 | 从多重混合物中识别个别样本的系统和方法 |
| WO2009017743A2 (en) | 2007-07-30 | 2009-02-05 | Argos Therapeutics, Inc. | Improved primers and probes for the amplification and detection of hiv gag, rev and nef polynucleotides |
| CA2699606C (en) | 2007-09-17 | 2023-04-11 | Oncomethylome Sciences Sa | Novel markers for bladder cancer detection |
| US20090099040A1 (en) * | 2007-10-15 | 2009-04-16 | Sigma Aldrich Company | Degenerate oligonucleotides and their uses |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| US20090291475A1 (en) * | 2008-04-23 | 2009-11-26 | Kai Qin Lao | Sequence amplification with linear primers |
| WO2009148560A2 (en) * | 2008-05-30 | 2009-12-10 | Board Of Regents, The Universtiy Of Texas System | Methods and compositions for nucleic acid sequencing |
| US20100028955A1 (en) * | 2008-07-22 | 2010-02-04 | Life Technologies Corporation | Sequence Amplification with Target Primers |
| JP5652843B2 (ja) * | 2008-10-17 | 2015-01-14 | 独立行政法人農業・食品産業技術総合研究機構 | Ｄｎａ増幅法 |
| US10669574B2 (en) | 2008-11-18 | 2020-06-02 | XCR Diagnostics, Inc. | DNA amplification technology |
| US8906621B2 (en) * | 2009-01-06 | 2014-12-09 | Qimin You | Cross priming amplification of target nucleic acids |
| CA2755358A1 (en) | 2009-03-13 | 2010-09-16 | Mdxhealth Sa | Novel markers for bladder cancer detection |
| US9524369B2 (en) * | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| FR2949120A1 (fr) * | 2009-08-13 | 2011-02-18 | Centre Nat Rech Scient | Procede de detection d'un adn circularise et utilisation de ce procede pour la detection de mutations |
| WO2011044437A2 (en) | 2009-10-09 | 2011-04-14 | Stc.Unm | Polony sequencing methods |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US20110195457A1 (en) * | 2010-02-09 | 2011-08-11 | General Electric Company | Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| DK201000356A (en) * | 2010-04-22 | 2011-10-23 | Statens Seruminstitut | New nucleotide sequence amplification Method |
| KR101334072B1 (ko) * | 2010-11-25 | 2013-11-29 | 한국표준과학연구원 | 핵산 정량 방법 및 키트 |
| US9738930B2 (en) | 2011-01-28 | 2017-08-22 | The Broad Institute, Inc. | Paired end bead amplification and high throughput sequencing |
| US9290815B2 (en) | 2011-02-21 | 2016-03-22 | Qiagen Gmbh | Method for quantifying human DNA |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US9617598B2 (en) | 2011-05-27 | 2017-04-11 | President And Fellows Of Harvard College | Methods of amplifying whole genome of a single cell |
| US10364464B2 (en) | 2011-08-08 | 2019-07-30 | The Broad Institute, Inc. | Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence |
| WO2013101783A2 (en) | 2011-12-30 | 2013-07-04 | Bio-Rad Laboratories, Inc. | Methods and compositions for performing nucleic acid amplification reactions |
| US10941396B2 (en) | 2012-02-27 | 2021-03-09 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
| WO2013138536A1 (en) | 2012-03-13 | 2013-09-19 | Swift Biosciences, Inc. | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
| HK1198836A1 (en) * | 2012-03-30 | 2015-06-12 | 深圳华大基因科技服务有限公司 | Whole genome amplification method and application thereof |
| US20150141258A1 (en) * | 2012-04-30 | 2015-05-21 | Qiagen Gmbh | Targeted dna enrichment and sequencing |
| EP2877593B1 (en) * | 2012-07-26 | 2018-07-18 | Illumina, Inc. | Compositions and methods for the amplification of nucleic acids |
| EP2895626B1 (en) * | 2012-09-12 | 2019-12-18 | The Regents of The University of California | Accurate genome sequencing of single cells by single-stranded amplification and sequencing |
| EP3901280B1 (en) | 2012-10-17 | 2025-03-12 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| EP2722399A1 (en) * | 2012-10-18 | 2014-04-23 | Roche Diagniostics GmbH | Method for preventing high molecular weight products during amplification |
| KR101451874B1 (ko) * | 2013-02-27 | 2014-10-16 | 국민대학교산학협력단 | 실시간 ｐｃｒ을 이용한 미생물의 최단시간 검출방법 |
| LT3013983T (lt) | 2013-06-25 | 2023-05-10 | Prognosys Biosciences, Inc. | Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį |
| EP3039158B1 (en) | 2013-08-28 | 2018-11-14 | Cellular Research, Inc. | Massively parallel single cell analysis |
| ES2925313T3 (es) | 2013-10-09 | 2022-10-14 | Fluoresentric Inc | Método para la detección de secuencias de ácido nucleico diana |
| CA2930942A1 (en) | 2013-11-18 | 2015-05-21 | Rubicon Genomics, Inc. | Degradable adaptors for background reduction |
| US9293316B2 (en) * | 2014-04-04 | 2016-03-22 | Thermo Finnigan Llc | Ion separation and storage system |
| CN105463585B (zh) * | 2014-09-12 | 2018-04-20 | 清华大学 | 基于单链dna分子构建测序文库的方法及其应用 |
| EP3208343B1 (en) * | 2014-10-13 | 2022-01-05 | MGI Tech Co., Ltd. | Nucleic acid fragmentation method and sequence combination |
| EP3208344B1 (en) * | 2014-10-17 | 2020-04-22 | MGI Tech Co., Ltd. | Primer for nucleic acid random fragmentation and nucleic acid random fragmentation method |
| WO2016138292A1 (en) | 2015-02-25 | 2016-09-01 | Igenomx International Genomics Corporation | Methods and compositions for in silico long read sequencing |
| US9727810B2 (en) | 2015-02-27 | 2017-08-08 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| JP7508191B2 (ja) | 2015-03-30 | 2024-07-01 | ベクトン・ディキンソン・アンド・カンパニー | コンビナトリアルバーコーディングのための方法および組成物 |
| WO2016160965A1 (en) | 2015-03-30 | 2016-10-06 | Rubicon Genomics, Inc. | Methods and compositions for repair of dna ends by multiple enzymatic activities |
| WO2016156529A1 (en) * | 2015-03-31 | 2016-10-06 | Qiagen Gmbh | Efficiency improving ligation methods |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| WO2016172373A1 (en) | 2015-04-23 | 2016-10-27 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
| ES2961374T3 (es) * | 2015-04-24 | 2024-03-11 | Atila Biosystems Incorporated | Amplificación con cebadores de composición de nucleótidos limitada |
| RU2018105835A (ru) | 2015-07-17 | 2019-08-19 | Президент Энд Фэллоуз Оф Харвард Коллидж | Способы амплификации последовательностей нуклеиновых кислот |
| WO2017021449A1 (en) * | 2015-08-06 | 2017-02-09 | F. Hoffmann-La Roche Ag | Target enrichment by single probe primer extension |
| CN105602939A (zh) * | 2015-09-02 | 2016-05-25 | 序康医疗科技（苏州）有限公司 | 扩增dna的方法 |
| KR102395450B1 (ko) | 2015-09-11 | 2022-05-09 | 셀룰러 리서치, 인크. | 핵산 라이브러리 정규화를 위한 방법 및 조성물 |
| CN105506125B (zh) * | 2016-01-12 | 2019-01-22 | 上海美吉生物医药科技有限公司 | 一种dna的测序方法及一种二代测序文库 |
| US11339427B2 (en) | 2016-02-12 | 2022-05-24 | Jumpcode Genomics, Inc. | Method for target specific RNA transcription of DNA sequences |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| JP7343264B2 (ja) * | 2016-06-29 | 2023-09-12 | トヨタ自動車株式会社 | Ｄｎａライブラリーの作製方法及びｄｎａライブラリーを用いたゲノムｄｎａ解析方法 |
| WO2018003220A1 (ja) * | 2016-06-29 | 2018-01-04 | トヨタ自動車株式会社 | Ｄｎａライブラリーの作製方法及びｄｎａライブラリーを用いたゲノムｄｎａ解析方法 |
| CN109477142B (zh) * | 2016-07-18 | 2022-03-22 | 豪夫迈·罗氏有限公司 | 不对称模板和核酸测序的不对称方法 |
| EP3507379A4 (en) * | 2016-08-31 | 2020-05-13 | President and Fellows of Harvard College | METHOD FOR DIGITAL TOTAL GENOM AMPLIFICATION |
| JP7001678B2 (ja) | 2016-09-06 | 2022-02-04 | スウィフト バイオサイエンシーズ， インコーポレイテッド | Ｎｇｓライブラリー濃度の正規化 |
| EP3516400B1 (en) | 2016-09-26 | 2023-08-16 | Becton, Dickinson and Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| WO2018061674A1 (ja) | 2016-09-30 | 2018-04-05 | 富士フイルム株式会社 | 脊椎動物由来の単一細胞の塩基配列情報を取得する方法 |
| US11319583B2 (en) | 2017-02-01 | 2022-05-03 | Becton, Dickinson And Company | Selective amplification using blocking oligonucleotides |
| JP7056012B2 (ja) | 2017-05-19 | 2022-04-19 | トヨタ自動車株式会社 | ランダムプライマーセット、及びこれを用いたｄｎａライブラリーの作製方法 |
| JP7536450B2 (ja) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | 単一細胞用のサンプルインデックス付加 |
| WO2019018561A1 (en) * | 2017-07-19 | 2019-01-24 | The Scripps Research Institute | GENOMIC LIBRARY GENERATION IN SOLID PHASE FOR HIGH FLOW SEQUENCING |
| WO2019033065A1 (en) | 2017-08-11 | 2019-02-14 | Atila Biosystems, Inc. | DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDE COMPOSITION PRIMERS |
| CA3084439A1 (en) * | 2017-12-07 | 2019-06-13 | Massachusetts Institute Of Technology | Single cell analyses |
| CN109971843B (zh) * | 2017-12-27 | 2022-11-11 | 复旦大学泰州健康科学研究院 | 一种单细胞转录组的测序方法 |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| CN118910215A (zh) | 2018-05-03 | 2024-11-08 | 贝克顿迪金森公司 | 在相对的转录物末端进行分子条形码化 |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| US11639517B2 (en) | 2018-10-01 | 2023-05-02 | Becton, Dickinson And Company | Determining 5′ transcript sequences |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| EP4567127A3 (en) | 2018-12-10 | 2025-09-03 | 10x Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| CN113195717A (zh) | 2018-12-13 | 2021-07-30 | 贝克顿迪金森公司 | 单细胞全转录组分析中的选择性延伸 |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| EP4242322B1 (en) | 2019-01-23 | 2024-08-21 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
| US12071617B2 (en) | 2019-02-14 | 2024-08-27 | Becton, Dickinson And Company | Hybrid targeted and whole transcriptome amplification |
| WO2020168239A1 (en) * | 2019-02-15 | 2020-08-20 | Takara Bio Usa, Inc. | Methods of preparing and analyzing nucleic acid libraries |
| WO2020191521A1 (zh) * | 2019-03-22 | 2020-10-01 | 深圳华大智造科技有限公司 | 核酸序列、rna目标区域测序文库的构建方法及应用 |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| EP3994262A4 (en) * | 2019-06-26 | 2023-09-20 | Stratos Genomics Inc. | Enhancement of nucleic acid polymerization by aromatic compounds |
| US11939622B2 (en) | 2019-07-22 | 2024-03-26 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
| GB201911515D0 (en) * | 2019-08-12 | 2019-09-25 | Univ London Queen Mary | Methods for generating a population of polynucleotide molecules |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| WO2021127406A1 (en) * | 2019-12-19 | 2021-06-24 | The Regents Of The University Of California | Methods of producing target capture nucleic acids |
| EP3891300B1 (en) | 2019-12-23 | 2023-03-29 | 10X Genomics, Inc. | Methods for spatial analysis using rna-templated ligation |
| US12241890B2 (en) | 2019-12-23 | 2025-03-04 | 10X Genomics, Inc. | Methods for generating barcoded nucleic acid molecules using fixed cells |
| WO2021146207A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and rna |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| ES2993319T3 (en) | 2020-01-29 | 2024-12-27 | Becton Dickinson Co | Barcoded wells for spatial mapping of single cells through sequencing |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| EP4242325B1 (en) | 2020-04-22 | 2025-01-29 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| US11702689B2 (en) * | 2020-04-24 | 2023-07-18 | Microsoft Technology Licensing, Llc | Homopolymer primers for amplification of polynucleotides created by enzymatic synthesis |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司（Us） | 用于免疫组库谱分析的引物 |
| EP4553168A3 (en) | 2020-05-22 | 2025-08-27 | 10x Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021236929A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| CN115803445A (zh) | 2020-06-02 | 2023-03-14 | 贝克顿迪金森公司 | 用于5撇基因表达测定的寡核苷酸和珠 |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4162074B1 (en) | 2020-06-08 | 2024-04-24 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| EP4172362B1 (en) | 2020-06-25 | 2024-09-18 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| WO2022109343A1 (en) | 2020-11-20 | 2022-05-27 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| EP4121555A1 (en) | 2020-12-21 | 2023-01-25 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US11280028B1 (en) | 2021-02-24 | 2022-03-22 | Agency For Science, Technology And Research (A*Star) | Unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid |
| WO2022221425A1 (en) | 2021-04-14 | 2022-10-20 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| ES3011462T3 (en) | 2021-09-01 | 2025-04-07 | 10X Genomics Inc | Methods for blocking a capture probe on a spatial array |
| EP4419707A1 (en) * | 2021-11-10 | 2024-08-28 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| DE102022211087A1 (de) * | 2022-10-19 | 2024-04-25 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches |
| EP4628594A1 (en) * | 2024-04-05 | 2025-10-08 | 4basebio UK Ltd | Methods of amplifying dna and dna products with inhibitory regions |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6114149A (en) * | 1988-07-26 | 2000-09-05 | Genelabs Technologies, Inc. | Amplification of mixed sequence nucleic acid fragments |
| US6280949B1 (en) * | 1997-10-08 | 2001-08-28 | Yale University | Multiple displacement amplification |
| US20020045169A1 (en) * | 2000-08-25 | 2002-04-18 | Shoemaker Daniel D. | Gene discovery using microarrays |
| EP1275738A1 (en) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Method for random cDNA synthesis and amplification |
Family Cites Families (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| AU622104B2 (en) | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
| WO1989006700A1 (en) | 1988-01-21 | 1989-07-27 | Genentech, Inc. | Amplification and detection of nucleic acid sequences |
| US6107023A (en) * | 1988-06-17 | 2000-08-22 | Genelabs Technologies, Inc. | DNA amplification and subtraction techniques |
| US4932207A (en) | 1988-12-28 | 1990-06-12 | Sundstrand Corporation | Segmented seal plate for a turbine engine |
| US5043272A (en) * | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5106727A (en) * | 1989-04-27 | 1992-04-21 | Life Technologies, Inc. | Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers |
| US6040138A (en) * | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
| US5104792A (en) * | 1989-12-21 | 1992-04-14 | The United States Of America As Represented By The Department Of Health And Human Services | Method for amplifying unknown nucleic acid sequences |
| ATE382094T1 (de) * | 1991-09-24 | 2008-01-15 | Keygene Nv | Primer, sätze und restriktions fragmente und dessen benutzung in selektive restriktionsfragmentenamplifikation |
| WO1993014217A1 (en) * | 1992-01-10 | 1993-07-22 | Life Technologies, Inc. | Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules |
| KR100249110B1 (ko) * | 1992-05-06 | 2000-04-01 | 다니엘 엘. 캐시앙 | 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트 |
| US5514545A (en) * | 1992-06-11 | 1996-05-07 | Trustees Of The University Of Pennsylvania | Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics |
| US5731171A (en) * | 1993-07-23 | 1998-03-24 | Arch Development Corp. | Sequence independent amplification of DNA |
| US5523204A (en) * | 1993-12-10 | 1996-06-04 | Becton Dickinson And Company | Detection of nucleic acids in cells by strand displacement amplification |
| US5710000A (en) * | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| GB9422891D0 (en) * | 1994-11-12 | 1995-01-04 | Univ Nottingham | Improvements in and relating to the amplification and identification of nucleotide sequences |
| JPH08173164A (ja) | 1994-12-22 | 1996-07-09 | Hitachi Ltd | Ｄｎａ調製法 |
| US5565340A (en) | 1995-01-27 | 1996-10-15 | Clontech Laboratories, Inc. | Method for suppressing DNA fragment amplification during PCR |
| US5994058A (en) * | 1995-03-20 | 1999-11-30 | Genome International Corporation | Method for contiguous genome sequencing |
| US5871820A (en) * | 1995-04-06 | 1999-02-16 | General Electric Company | Protection of thermal barrier coating with an impermeable barrier coating |
| US5750341A (en) * | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5648245A (en) | 1995-05-09 | 1997-07-15 | Carnegie Institution Of Washington | Method for constructing an oligonucleotide concatamer library by rolling circle replication |
| US5814444A (en) * | 1995-06-07 | 1998-09-29 | University Of Washington | Methods for making and using single-chromosome amplfication libraries |
| WO1998004689A1 (en) | 1995-07-31 | 1998-02-05 | Urocor, Inc. | Biomarkers and targets for diagnosis, prognosis and management of prostate disease |
| US5631147A (en) * | 1995-09-21 | 1997-05-20 | Becton, Dickinson And Company | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
| EP0880598A4 (en) * | 1996-01-23 | 2005-02-23 | Affymetrix Inc | RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM |
| ATE284891T1 (de) * | 1996-02-14 | 2005-01-15 | Biomerieux Bv | Isolierung von einzelsträngigen nukleinsäuren |
| DE69736475T2 (de) * | 1996-05-02 | 2007-08-16 | Applera Corp., Foster City | Quantifizierung von rna-transkripten mittels genomischer dna als internen standard der amplifizierungsreaktion |
| AU6497296A (en) | 1996-07-16 | 1998-02-09 | Periannan Senapathy | Method for contiguous genome sequencing |
| EP0835935A1 (en) * | 1996-10-03 | 1998-04-15 | Roche Diagnostics GmbH | Thermostable DNA Polymerase from Anaerocellum thermophilum |
| US6030814A (en) * | 1997-04-21 | 2000-02-29 | Epicentre Technologies Corporation | Reverse transcription method |
| US5932451A (en) * | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
| DE19813317A1 (de) | 1998-03-26 | 1999-09-30 | Roche Diagnostics Gmbh | Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR |
| US6232067B1 (en) * | 1998-08-17 | 2001-05-15 | The Perkin-Elmer Corporation | Adapter directed expression analysis |
| DK1109938T3 (da) | 1998-09-18 | 2002-05-27 | Micromet Ag | DNA-amplificering af en enkelt celle |
| US6506594B1 (en) * | 1999-03-19 | 2003-01-14 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US6521428B1 (en) * | 1999-04-21 | 2003-02-18 | Genome Technologies, Llc | Shot-gun sequencing and amplification without cloning |
| US6491752B1 (en) | 1999-07-16 | 2002-12-10 | Sumco Oregon Corporation | Enhanced n-type silicon material for epitaxial wafer substrate and method of making same |
| AU6387000A (en) | 1999-07-29 | 2001-02-19 | Genzyme Corporation | Serial analysis of genetic alterations |
| US6846626B1 (en) * | 1999-09-01 | 2005-01-25 | Genome Technologies, Llc | Method for amplifying sequences from unknown DNA |
| US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| US6271002B1 (en) | 1999-10-04 | 2001-08-07 | Rosetta Inpharmatics, Inc. | RNA amplification method |
| US6958225B2 (en) | 1999-10-27 | 2005-10-25 | Affymetrix, Inc. | Complexity management of genomic DNA |
| EP1276901A2 (en) * | 2000-01-13 | 2003-01-22 | Amsterdam Support Diagnostics B.V. | A universal nucleic acid amplification system for nucleic acids in a sample |
| US6379932B1 (en) | 2000-07-17 | 2002-04-30 | Incyte Genomics, Inc. | Single primer PCR amplification of RNA |
| WO2002020843A1 (en) * | 2000-09-06 | 2002-03-14 | Rutgers, The State University | A method for sequencing nucleic acid sequences without chain termination |
| AU2002213882A1 (en) | 2000-09-08 | 2002-03-22 | Biomerieux B.V.A. | Attenuated hiv strains and use thereof |
| US6794141B2 (en) | 2000-12-22 | 2004-09-21 | Arcturus Bioscience, Inc. | Nucleic acid amplification |
| WO2002061140A2 (en) * | 2001-01-31 | 2002-08-08 | Ambion, Inc. | Competitive population normalization for comparative analysis of nucleic acid samples |
| JP2004527236A (ja) | 2001-02-14 | 2004-09-09 | ベイラー カレッジ オブ メディスン | Ｒｎａ増幅の方法及び組成物 |
| CN1289690C (zh) | 2001-03-09 | 2006-12-13 | 纽亘技术公司 | Rna序列扩增的方法和组合物 |
| WO2002103054A1 (en) | 2001-05-02 | 2002-12-27 | Rubicon Genomics Inc. | Genome walking by selective amplification of nick-translate dna library and amplification from complex mixtures of templates |
| JP2002345466A (ja) | 2001-05-08 | 2002-12-03 | Takara Bio Inc | Ｄｎａの増幅方法 |
| US6638722B2 (en) * | 2001-06-13 | 2003-10-28 | Invitrogen Corporation | Method for rapid amplification of DNA |
| EP1275734A1 (en) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Method for random cDNA synthesis and amplification |
| US20040175715A1 (en) | 2001-08-21 | 2004-09-09 | Burgoyne Leigh A. | Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genomes mixtures |
| US20040019005A1 (en) * | 2001-10-01 | 2004-01-29 | Jeffrey Van Ness | Methods for parallel measurement of genetic variations |
| AU2002359436A1 (en) | 2001-11-13 | 2003-06-23 | Rubicon Genomics Inc. | Dna amplification and sequencing using dna molecules generated by random fragmentation |
| US20030211528A1 (en) * | 2002-03-12 | 2003-11-13 | Norman Iscove | Exponential amplification of sub-picogram nucleic acid samples with retention of quantitative representation |
| KR100545295B1 (ko) * | 2003-09-29 | 2006-01-24 | 현대모비스 주식회사 | 차량용 조향장치의 너클 어셈블리 |
| US8440404B2 (en) * | 2004-03-08 | 2013-05-14 | Rubicon Genomics | Methods and compositions for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation |
| US8312249B1 (en) | 2008-10-10 | 2012-11-13 | Apple Inc. | Dynamic trampoline and structured code generation in a signed code environment |
| FR2957821B1 (fr) | 2010-03-24 | 2014-08-29 | Inst Francais Du Petrole | Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives |
-
2004
- 2004-03-08 DK DK10011482.6T patent/DK2374900T3/en active
- 2004-03-08 DK DK04718499.9T patent/DK1604040T3/da active
- 2004-03-08 DE DE602004029560T patent/DE602004029560D1/de not_active Expired - Lifetime
- 2004-03-08 WO PCT/US2004/006983 patent/WO2004081225A2/en not_active Ceased
- 2004-03-08 EP EP04718499A patent/EP1604040B1/en not_active Expired - Lifetime
- 2004-03-08 US US10/795,667 patent/US7718403B2/en active Active
- 2004-03-08 EP EP10011482.6A patent/EP2374900B1/en not_active Expired - Lifetime
- 2004-03-08 AT AT04718499T patent/ATE484598T1/de not_active IP Right Cessation
- 2004-03-08 JP JP2006509236A patent/JP4773338B2/ja not_active Expired - Lifetime
-
2006
- 2006-11-06 US US11/593,625 patent/US20070054311A1/en not_active Abandoned
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6114149A (en) * | 1988-07-26 | 2000-09-05 | Genelabs Technologies, Inc. | Amplification of mixed sequence nucleic acid fragments |
| US6280949B1 (en) * | 1997-10-08 | 2001-08-28 | Yale University | Multiple displacement amplification |
| US20020045169A1 (en) * | 2000-08-25 | 2002-04-18 | Shoemaker Daniel D. | Gene discovery using microarrays |
| EP1275738A1 (en) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Method for random cDNA synthesis and amplification |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1604040A2 (en) | 2005-12-14 |
| ATE484598T1 (de) | 2010-10-15 |
| EP1604040B1 (en) | 2010-10-13 |
| EP2374900B1 (en) | 2016-07-13 |
| DK2374900T3 (en) | 2016-10-17 |
| WO2004081225A2 (en) | 2004-09-23 |
| US7718403B2 (en) | 2010-05-18 |
| US20040209298A1 (en) | 2004-10-21 |
| US20070054311A1 (en) | 2007-03-08 |
| EP2374900A1 (en) | 2011-10-12 |
| HK1089485A1 (en) | 2006-12-01 |
| JP2006519621A (ja) | 2006-08-31 |
| DK1604040T3 (da) | 2011-01-24 |
| DE602004029560D1 (de) | 2010-11-25 |
| WO2004081225A3 (en) | 2004-11-25 |
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