JP4773338B2 - Dna重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析 - Google Patents
Dna重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析 Download PDFInfo
- Publication number
- JP4773338B2 JP4773338B2 JP2006509236A JP2006509236A JP4773338B2 JP 4773338 B2 JP4773338 B2 JP 4773338B2 JP 2006509236 A JP2006509236 A JP 2006509236A JP 2006509236 A JP2006509236 A JP 2006509236A JP 4773338 B2 JP4773338 B2 JP 4773338B2
- Authority
- JP
- Japan
- Prior art keywords
- dna
- primer
- amplification
- polymerase
- primers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000006116 polymerization reaction Methods 0.000 title claims description 14
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract description 327
- 230000003321 amplification Effects 0.000 title abstract description 324
- 238000004458 analytical method Methods 0.000 title description 43
- 238000000034 method Methods 0.000 claims abstract description 279
- 150000007523 nucleic acids Chemical group 0.000 claims abstract description 168
- 230000000295 complement effect Effects 0.000 claims abstract description 121
- 239000000203 mixture Substances 0.000 claims abstract description 119
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 153
- 102000039446 nucleic acids Human genes 0.000 claims description 123
- 108020004707 nucleic acids Proteins 0.000 claims description 123
- 238000003752 polymerase chain reaction Methods 0.000 claims description 119
- 108020004414 DNA Proteins 0.000 claims description 82
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 77
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 76
- 102000053602 DNA Human genes 0.000 claims description 74
- 108010017826 DNA Polymerase I Proteins 0.000 claims description 61
- 102000004594 DNA Polymerase I Human genes 0.000 claims description 61
- 125000003729 nucleotide group Chemical group 0.000 claims description 59
- 239000002773 nucleotide Substances 0.000 claims description 57
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 53
- 230000000694 effects Effects 0.000 claims description 49
- 238000009396 hybridization Methods 0.000 claims description 49
- 102100034343 Integrase Human genes 0.000 claims description 44
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 40
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 39
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims description 38
- 230000008569 process Effects 0.000 claims description 36
- 229920001519 homopolymer Polymers 0.000 claims description 32
- 241000713869 Moloney murine leukemia virus Species 0.000 claims description 28
- 238000012163 sequencing technique Methods 0.000 claims description 27
- 229940104302 cytosine Drugs 0.000 claims description 20
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 claims description 19
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 claims description 19
- 229940104230 thymidine Drugs 0.000 claims description 19
- 238000002493 microarray Methods 0.000 claims description 18
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 17
- 229930024421 Adenine Natural products 0.000 claims description 17
- 229960000643 adenine Drugs 0.000 claims description 17
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 15
- 238000010348 incorporation Methods 0.000 claims description 13
- 108060002716 Exonuclease Proteins 0.000 claims description 12
- 102000013165 exonuclease Human genes 0.000 claims description 12
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 claims description 11
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 9
- -1 9 ° Nm polymerase Proteins 0.000 claims description 8
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 claims description 7
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims description 7
- 101710176276 SSB protein Proteins 0.000 claims description 7
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims description 6
- 108060004795 Methyltransferase Proteins 0.000 claims description 6
- 108010078851 HIV Reverse Transcriptase Proteins 0.000 claims description 5
- 108010001244 Tli polymerase Proteins 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- 108010010677 Phosphodiesterase I Proteins 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 101710116602 DNA-Binding protein G5P Proteins 0.000 claims description 3
- 101710162453 Replication factor A Proteins 0.000 claims description 3
- 101710176758 Replication protein A 70 kDa DNA-binding subunit Proteins 0.000 claims description 3
- 230000001404 mediated effect Effects 0.000 claims description 3
- 230000002285 radioactive effect Effects 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 4
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims 1
- 229960003237 betaine Drugs 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 44
- 238000013412 genome amplification Methods 0.000 abstract description 42
- 102000040430 polynucleotide Human genes 0.000 abstract description 28
- 108091033319 polynucleotide Proteins 0.000 abstract description 28
- 239000002157 polynucleotide Substances 0.000 abstract description 28
- 239000013615 primer Substances 0.000 description 670
- 238000006243 chemical reaction Methods 0.000 description 133
- 239000000523 sample Substances 0.000 description 100
- 210000004027 cell Anatomy 0.000 description 99
- 239000012634 fragment Substances 0.000 description 88
- 238000003786 synthesis reaction Methods 0.000 description 83
- 238000003753 real-time PCR Methods 0.000 description 74
- 239000000047 product Substances 0.000 description 70
- 230000015572 biosynthetic process Effects 0.000 description 56
- 230000000875 corresponding effect Effects 0.000 description 50
- 230000037452 priming Effects 0.000 description 42
- 238000006073 displacement reaction Methods 0.000 description 38
- 108091034117 Oligonucleotide Proteins 0.000 description 37
- 239000000872 buffer Substances 0.000 description 36
- 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 description 35
- 230000014509 gene expression Effects 0.000 description 32
- 239000011541 reaction mixture Substances 0.000 description 32
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 31
- 229910052719 titanium Inorganic materials 0.000 description 31
- 239000010936 titanium Substances 0.000 description 31
- 108090000623 proteins and genes Proteins 0.000 description 29
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 28
- 108010006785 Taq Polymerase Proteins 0.000 description 28
- 238000010438 heat treatment Methods 0.000 description 28
- 238000000137 annealing Methods 0.000 description 27
- 239000000539 dimer Substances 0.000 description 25
- 239000000463 material Substances 0.000 description 25
- 108020004999 messenger RNA Proteins 0.000 description 25
- 238000004925 denaturation Methods 0.000 description 24
- 230000036425 denaturation Effects 0.000 description 24
- 238000010790 dilution Methods 0.000 description 24
- 239000012895 dilution Substances 0.000 description 24
- 238000011534 incubation Methods 0.000 description 24
- 102000004190 Enzymes Human genes 0.000 description 23
- 108090000790 Enzymes Proteins 0.000 description 23
- 238000009826 distribution Methods 0.000 description 22
- 210000002966 serum Anatomy 0.000 description 21
- 238000002360 preparation method Methods 0.000 description 20
- 230000010076 replication Effects 0.000 description 20
- 230000027455 binding Effects 0.000 description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
- 239000003068 molecular probe Substances 0.000 description 18
- 239000011535 reaction buffer Substances 0.000 description 18
- 108091008146 restriction endonucleases Proteins 0.000 description 18
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 17
- 230000001965 increasing effect Effects 0.000 description 17
- 210000001519 tissue Anatomy 0.000 description 17
- 108091093088 Amplicon Proteins 0.000 description 16
- 241000588724 Escherichia coli Species 0.000 description 16
- 210000000349 chromosome Anatomy 0.000 description 16
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 16
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 15
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 14
- 101000661600 Homo sapiens Steryl-sulfatase Proteins 0.000 description 14
- 102000054458 human STS Human genes 0.000 description 14
- 238000011529 RT qPCR Methods 0.000 description 13
- 238000013459 approach Methods 0.000 description 13
- 238000013467 fragmentation Methods 0.000 description 13
- 238000006062 fragmentation reaction Methods 0.000 description 13
- 230000002829 reductive effect Effects 0.000 description 13
- 238000012408 PCR amplification Methods 0.000 description 12
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 12
- 210000004369 blood Anatomy 0.000 description 12
- 239000008280 blood Substances 0.000 description 12
- 238000001514 detection method Methods 0.000 description 12
- 239000003550 marker Substances 0.000 description 11
- 238000010839 reverse transcription Methods 0.000 description 11
- 230000008685 targeting Effects 0.000 description 11
- 230000004544 DNA amplification Effects 0.000 description 10
- 239000002299 complementary DNA Substances 0.000 description 10
- 230000001419 dependent effect Effects 0.000 description 10
- 210000003780 hair follicle Anatomy 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 238000010561 standard procedure Methods 0.000 description 10
- 238000002955 isolation Methods 0.000 description 9
- 239000011780 sodium chloride Substances 0.000 description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
- 206010028980 Neoplasm Diseases 0.000 description 8
- 238000013461 design Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 8
- 238000010195 expression analysis Methods 0.000 description 8
- 150000003839 salts Chemical class 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 238000013518 transcription Methods 0.000 description 8
- 230000035897 transcription Effects 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000010222 PCR analysis Methods 0.000 description 7
- 108010017842 Telomerase Proteins 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- 201000011510 cancer Diseases 0.000 description 7
- 238000003745 diagnosis Methods 0.000 description 7
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 7
- 230000006872 improvement Effects 0.000 description 7
- 210000004698 lymphocyte Anatomy 0.000 description 7
- 239000007787 solid Substances 0.000 description 7
- 238000004448 titration Methods 0.000 description 7
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 6
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 6
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
- 101710203526 Integrase Proteins 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000003776 cleavage reaction Methods 0.000 description 6
- 238000003205 genotyping method Methods 0.000 description 6
- 229910052739 hydrogen Inorganic materials 0.000 description 6
- 230000000670 limiting effect Effects 0.000 description 6
- 239000012139 lysis buffer Substances 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 230000007017 scission Effects 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 238000010804 cDNA synthesis Methods 0.000 description 5
- 230000003915 cell function Effects 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 210000005260 human cell Anatomy 0.000 description 5
- 210000003917 human chromosome Anatomy 0.000 description 5
- 239000001257 hydrogen Substances 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 230000002779 inactivation Effects 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 238000011002 quantification Methods 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 230000001629 suppression Effects 0.000 description 5
- 108091035539 telomere Proteins 0.000 description 5
- 102000055501 telomere Human genes 0.000 description 5
- 210000003411 telomere Anatomy 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 239000013598 vector Substances 0.000 description 5
- 230000006820 DNA synthesis Effects 0.000 description 4
- 108010067770 Endopeptidase K Proteins 0.000 description 4
- 101710093976 Plasmid-derived single-stranded DNA-binding protein Proteins 0.000 description 4
- 239000013614 RNA sample Substances 0.000 description 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 4
- 239000007984 Tris EDTA buffer Substances 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 210000001766 X chromosome Anatomy 0.000 description 4
- 239000011543 agarose gel Substances 0.000 description 4
- 238000001574 biopsy Methods 0.000 description 4
- 229960002685 biotin Drugs 0.000 description 4
- 235000020958 biotin Nutrition 0.000 description 4
- 239000011616 biotin Substances 0.000 description 4
- 210000000601 blood cell Anatomy 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 230000003111 delayed effect Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000000977 initiatory effect Effects 0.000 description 4
- 239000002987 primer (paints) Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 150000003230 pyrimidines Chemical class 0.000 description 4
- 230000003252 repetitive effect Effects 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 108091023043 Alu Element Proteins 0.000 description 3
- 102000012410 DNA Ligases Human genes 0.000 description 3
- 108010061982 DNA Ligases Proteins 0.000 description 3
- 241000701867 Enterobacteria phage T7 Species 0.000 description 3
- 208000009889 Herpes Simplex Diseases 0.000 description 3
- 102000003960 Ligases Human genes 0.000 description 3
- 108090000364 Ligases Proteins 0.000 description 3
- 102000006382 Ribonucleases Human genes 0.000 description 3
- 108010083644 Ribonucleases Proteins 0.000 description 3
- 230000004075 alteration Effects 0.000 description 3
- 208000036878 aneuploidy Diseases 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 230000001351 cycling effect Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 238000011901 isothermal amplification Methods 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 210000003463 organelle Anatomy 0.000 description 3
- 150000002972 pentoses Chemical class 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 238000003793 prenatal diagnosis Methods 0.000 description 3
- 230000001915 proofreading effect Effects 0.000 description 3
- 150000003212 purines Chemical class 0.000 description 3
- 238000011897 real-time detection Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 230000003362 replicative effect Effects 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 229940113082 thymine Drugs 0.000 description 3
- 230000005945 translocation Effects 0.000 description 3
- 201000008827 tuberculosis Diseases 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 101150026402 DBP gene Proteins 0.000 description 2
- 238000007399 DNA isolation Methods 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 2
- 239000003155 DNA primer Substances 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 108091092584 GDNA Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 101710128836 Large T antigen Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 108091092878 Microsatellite Proteins 0.000 description 2
- 102000011931 Nucleoproteins Human genes 0.000 description 2
- 108010061100 Nucleoproteins Proteins 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 102000043276 Oncogene Human genes 0.000 description 2
- 108091093037 Peptide nucleic acid Proteins 0.000 description 2
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical group [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 2
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 2
- 108010066717 Q beta Replicase Proteins 0.000 description 2
- 238000002123 RNA extraction Methods 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 108091081021 Sense strand Proteins 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 241000193998 Streptococcus pneumoniae Species 0.000 description 2
- 101000865057 Thermococcus litoralis DNA polymerase Proteins 0.000 description 2
- 101710183280 Topoisomerase Proteins 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 231100001075 aneuploidy Toxicity 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 2
- 238000012742 biochemical analysis Methods 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 230000003628 erosive effect Effects 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 238000012252 genetic analysis Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000001531 micro-dissection Methods 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000006548 oncogenic transformation Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 239000013610 patient sample Substances 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 210000002307 prostate Anatomy 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 230000009758 senescence Effects 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- 108010068698 spleen exonuclease Proteins 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 238000005382 thermal cycling Methods 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- MWBWWFOAEOYUST-UHFFFAOYSA-N 2-aminopurine Chemical compound NC1=NC=C2N=CNC2=N1 MWBWWFOAEOYUST-UHFFFAOYSA-N 0.000 description 1
- ZLHLYESIHSHXGM-UHFFFAOYSA-N 4,6-dimethyl-1h-imidazo[1,2-a]purin-9-one Chemical compound N=1C(C)=CN(C2=O)C=1N(C)C1=C2NC=N1 ZLHLYESIHSHXGM-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- LQLQRFGHAALLLE-UHFFFAOYSA-N 5-bromouracil Chemical compound BrC1=CNC(=O)NC1=O LQLQRFGHAALLLE-UHFFFAOYSA-N 0.000 description 1
- 108700015125 Adenovirus DBP Proteins 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 108091029845 Aminoallyl nucleotide Proteins 0.000 description 1
- 241000242757 Anthozoa Species 0.000 description 1
- 101150062763 BMRF1 gene Proteins 0.000 description 1
- 241000322342 Bacillus phage M2 Species 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 235000014653 Carica parviflora Nutrition 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 208000037051 Chromosomal Instability Diseases 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108020005031 Concatenated DNA Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 108050009160 DNA polymerase 1 Proteins 0.000 description 1
- 101710134178 DNA polymerase processivity factor BMRF1 Proteins 0.000 description 1
- 101710096438 DNA-binding protein Proteins 0.000 description 1
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 101100070287 Enterobacteria phage T4 41 gene Proteins 0.000 description 1
- 101000643395 Escherichia phage T7 Single-stranded DNA-binding protein Proteins 0.000 description 1
- 241000701533 Escherichia virus T4 Species 0.000 description 1
- 241000701988 Escherichia virus T5 Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 241000064099 Massilioclostridium coli Species 0.000 description 1
- 101500006448 Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) Endonuclease PI-MboI Proteins 0.000 description 1
- OKIZCWYLBDKLSU-UHFFFAOYSA-M N,N,N-Trimethylmethanaminium chloride Chemical compound [Cl-].C[N+](C)(C)C OKIZCWYLBDKLSU-UHFFFAOYSA-M 0.000 description 1
- WGZDBVOTUVNQFP-UHFFFAOYSA-N N-(1-phthalazinylamino)carbamic acid ethyl ester Chemical compound C1=CC=C2C(NNC(=O)OCC)=NN=CC2=C1 WGZDBVOTUVNQFP-UHFFFAOYSA-N 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000702212 Pseudomonas phage Pf3 Species 0.000 description 1
- 108010019653 Pwo polymerase Proteins 0.000 description 1
- 240000001749 Quercus lobata Species 0.000 description 1
- 235000013400 Quercus lobata Nutrition 0.000 description 1
- 101150085800 RPA2 gene Proteins 0.000 description 1
- 101150030992 RPA3 gene Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000018780 Replication Protein A Human genes 0.000 description 1
- 108010027643 Replication Protein A Proteins 0.000 description 1
- 102100034372 Replication protein A 14 kDa subunit Human genes 0.000 description 1
- 102100035525 Replication protein A 32 kDa subunit Human genes 0.000 description 1
- 108010081734 Ribonucleoproteins Proteins 0.000 description 1
- 102000004389 Ribonucleoproteins Human genes 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 101100528939 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) RPA14 gene Proteins 0.000 description 1
- 101150104425 T4 gene Proteins 0.000 description 1
- 102000010823 Telomere-Binding Proteins Human genes 0.000 description 1
- 108010038599 Telomere-Binding Proteins Proteins 0.000 description 1
- 241000589500 Thermus aquaticus Species 0.000 description 1
- 102100036407 Thioredoxin Human genes 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 108091061763 Triple-stranded DNA Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 208000037280 Trisomy Diseases 0.000 description 1
- 101150008036 UL29 gene Proteins 0.000 description 1
- 101100033868 Xenopus laevis rpa2-a gene Proteins 0.000 description 1
- 101100033871 Xenopus laevis rpa2-b gene Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 150000001408 amides Chemical group 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000003322 aneuploid effect Effects 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000012820 cell cycle checkpoint Effects 0.000 description 1
- 230000006369 cell cycle progression Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000030944 contact inhibition Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 229940079920 digestives acid preparations Drugs 0.000 description 1
- 239000013024 dilution buffer Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010448 genetic screening Methods 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- IIRDTKBZINWQAW-UHFFFAOYSA-N hexaethylene glycol Chemical compound OCCOCCOCCOCCOCCOCCO IIRDTKBZINWQAW-UHFFFAOYSA-N 0.000 description 1
- 239000000710 homodimer Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000001001 laser micro-dissection Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 238000007854 ligation-mediated PCR Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical compound CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 1
- 238000010208 microarray analysis Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000007857 nested PCR Methods 0.000 description 1
- 210000002445 nipple Anatomy 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 238000009595 pap smear Methods 0.000 description 1
- LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 210000000745 plant chromosome Anatomy 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 210000003055 polytene chromosome Anatomy 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- IGFXRKMLLMBKSA-UHFFFAOYSA-N purine Chemical compound N1=C[N]C2=NC=NC2=C1 IGFXRKMLLMBKSA-UHFFFAOYSA-N 0.000 description 1
- 125000000714 pyrimidinyl group Chemical group 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000022983 regulation of cell cycle Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012340 reverse transcriptase PCR Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000001963 scanning near-field photolithography Methods 0.000 description 1
- 238000002602 scintillography Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 230000008684 selective degradation Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 108700014590 single-stranded DNA binding proteins Proteins 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 108060008226 thioredoxin Proteins 0.000 description 1
- 229940094937 thioredoxin Drugs 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US45306003P | 2003-03-07 | 2003-03-07 | |
| US60/453,060 | 2003-03-07 | ||
| PCT/US2004/006983 WO2004081225A2 (en) | 2003-03-07 | 2004-03-08 | Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006519621A JP2006519621A (ja) | 2006-08-31 |
| JP2006519621A5 JP2006519621A5 (enExample) | 2007-04-05 |
| JP4773338B2 true JP4773338B2 (ja) | 2011-09-14 |
Family
ID=32990716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006509236A Expired - Lifetime JP4773338B2 (ja) | 2003-03-07 | 2004-03-08 | Dna重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US7718403B2 (enExample) |
| EP (2) | EP2374900B1 (enExample) |
| JP (1) | JP4773338B2 (enExample) |
| AT (1) | ATE484598T1 (enExample) |
| DE (1) | DE602004029560D1 (enExample) |
| DK (2) | DK2374900T3 (enExample) |
| WO (1) | WO2004081225A2 (enExample) |
Families Citing this family (172)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040024193A1 (en) * | 2002-07-26 | 2004-02-05 | Williams Gregg T. | Method of detecting and quantifying hepatitis C virus |
| US20090124514A1 (en) * | 2003-02-26 | 2009-05-14 | Perlegen Sciences, Inc. | Selection probe amplification |
| US20060183132A1 (en) * | 2005-02-14 | 2006-08-17 | Perlegen Sciences, Inc. | Selection probe amplification |
| US8206913B1 (en) * | 2003-03-07 | 2012-06-26 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
| US20050181394A1 (en) * | 2003-06-20 | 2005-08-18 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| JP2007525963A (ja) | 2003-06-20 | 2007-09-13 | イルミナ インコーポレイテッド | 全ゲノム増幅および遺伝型決定のための方法および組成物 |
| US7459274B2 (en) * | 2004-03-02 | 2008-12-02 | Orion Genomics Llc | Differential enzymatic fragmentation by whole genome amplification |
| JP3972106B2 (ja) * | 2004-03-03 | 2007-09-05 | 大学共同利用機関法人情報・システム研究機構 | ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー |
| CA2902980A1 (en) | 2004-03-08 | 2005-09-29 | Rubicon Genomics, Inc. | Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation |
| KR101450497B1 (ko) * | 2004-09-14 | 2014-10-13 | 아고스 쎄라퓨틱스, 인코포레이티드 | 병원체의 균주 독립성 증폭 및 그에 대한 백신 |
| US20080003602A1 (en) * | 2004-12-23 | 2008-01-03 | Ge Healthcare Bio-Sciences Corp. | Ligation-Based Rna Amplification |
| EP1848819A4 (en) * | 2005-02-16 | 2010-01-06 | Genetic Technologies Ltd | GENETIC ANALYSIS METHODS COMPRISING THE AMPLIFICATION OF COMPLEMENTARY DUPLICONS |
| US7449297B2 (en) | 2005-04-14 | 2008-11-11 | Euclid Diagnostics Llc | Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays |
| EP1907571B1 (en) | 2005-06-15 | 2017-04-26 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
| US20070003938A1 (en) * | 2005-06-30 | 2007-01-04 | Perlegen Sciences, Inc. | Hybridization of genomic nucleic acid without complexity reduction |
| WO2007018601A1 (en) | 2005-08-02 | 2007-02-15 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction |
| EP1924705A1 (en) | 2005-08-02 | 2008-05-28 | Rubicon Genomics, Inc. | Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method |
| WO2007019444A2 (en) * | 2005-08-05 | 2007-02-15 | Euclid Diagnostics Llc | Subtractive separation and amplification of non-ribosomal transcribed rna (nrrna) |
| WO2007035742A2 (en) * | 2005-09-16 | 2007-03-29 | 454 Life Sciences Corporation | Cdna library preparation |
| JP5457673B2 (ja) * | 2005-09-20 | 2014-04-02 | ベリデックス・リミテッド・ライアビリティ・カンパニー | ユニーク配列のdnaプローブを作製するための方法および組成物、dnaプローブの標識、ならびにこれらプローブの使用 |
| US9134237B2 (en) * | 2005-09-20 | 2015-09-15 | Janssen Diagnotics, LLC | High sensitivity multiparameter method for rare event analysis in a biological sample |
| US20070092899A1 (en) * | 2005-10-21 | 2007-04-26 | The Regents Of The University Of California | Multiple displacement amplification with blocker DNA |
| AU2006339538A1 (en) | 2005-11-08 | 2007-09-13 | Euclid Diagnostics Llc | Materials and methods for assaying for methylation of CpG islands associated with genes in the evaluation of cancer |
| US20080057499A1 (en) * | 2006-02-06 | 2008-03-06 | Affymetrix, Inc. | Methods for high specificity whole genome amplification and hybridization |
| US20090191556A1 (en) * | 2006-04-12 | 2009-07-30 | Medical Research Council | Method |
| WO2007136874A2 (en) * | 2006-05-18 | 2007-11-29 | President And Fellows Of Harvard College | Genomic library construction |
| US20080026390A1 (en) * | 2006-06-14 | 2008-01-31 | Roland Stoughton | Diagnosis of Fetal Abnormalities by Comparative Genomic Hybridization Analysis |
| WO2008016988A1 (en) * | 2006-08-01 | 2008-02-07 | Gen-Probe Incorporated | Methods of nonspecific target capture of nucleic acids |
| WO2008143627A2 (en) * | 2006-09-14 | 2008-11-27 | Ibis Biosciences, Inc. | Targeted whole genome amplification method for identification of pathogens |
| CN103952470B (zh) * | 2007-03-28 | 2017-11-10 | 信号诊断公司 | 高解析度分析核酸以检测序列变异的系统和方法 |
| US7794985B2 (en) * | 2007-04-04 | 2010-09-14 | Ghc Technologies, Inc. | Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins |
| EP2164985A4 (en) * | 2007-06-01 | 2014-05-14 | 454 Life Sciences Corp | SYSTEM AND METHOD FOR IDENTIFYING INDIVIDUAL SAMPLES FROM A MULTIPLEX MIXTURE |
| US8221981B2 (en) * | 2007-07-30 | 2012-07-17 | Argos Therapeutics, Inc. | Primers and probes for the amplification and detection of HIV Gag, Rev and Nef polynucleotides |
| DK2198042T3 (en) | 2007-09-17 | 2017-01-23 | Mdxhealth Sa | New markers for bladder cancer detection |
| US20090099040A1 (en) * | 2007-10-15 | 2009-04-16 | Sigma Aldrich Company | Degenerate oligonucleotides and their uses |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| US20090291475A1 (en) * | 2008-04-23 | 2009-11-26 | Kai Qin Lao | Sequence amplification with linear primers |
| WO2009148560A2 (en) * | 2008-05-30 | 2009-12-10 | Board Of Regents, The Universtiy Of Texas System | Methods and compositions for nucleic acid sequencing |
| US20100028955A1 (en) * | 2008-07-22 | 2010-02-04 | Life Technologies Corporation | Sequence Amplification with Target Primers |
| JP5652843B2 (ja) * | 2008-10-17 | 2015-01-14 | 独立行政法人農業・食品産業技術総合研究機構 | Dna増幅法 |
| US10669574B2 (en) | 2008-11-18 | 2020-06-02 | XCR Diagnostics, Inc. | DNA amplification technology |
| WO2010080691A1 (en) * | 2009-01-06 | 2010-07-15 | Qimin You | Cross priming amplification of target nucleic acids |
| CA2755358A1 (en) | 2009-03-13 | 2010-09-16 | Mdxhealth Sa | Novel markers for bladder cancer detection |
| US9524369B2 (en) * | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| FR2949120A1 (fr) * | 2009-08-13 | 2011-02-18 | Centre Nat Rech Scient | Procede de detection d'un adn circularise et utilisation de ce procede pour la detection de mutations |
| WO2011044437A2 (en) * | 2009-10-09 | 2011-04-14 | Stc.Unm | Polony sequencing methods |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US20110195457A1 (en) * | 2010-02-09 | 2011-08-11 | General Electric Company | Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| DK201000356A (en) * | 2010-04-22 | 2011-10-23 | Statens Seruminstitut | New nucleotide sequence amplification Method |
| KR101334072B1 (ko) * | 2010-11-25 | 2013-11-29 | 한국표준과학연구원 | 핵산 정량 방법 및 키트 |
| EP2668294B1 (en) | 2011-01-28 | 2021-04-07 | The Broad Institute, Inc. | Paired end bead amplification and high throughput sequencing |
| EP2678445B1 (en) | 2011-02-21 | 2014-12-17 | Qiagen GmbH | Method for quantifying human dna |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| EP2714938B1 (en) * | 2011-05-27 | 2017-11-15 | President and Fellows of Harvard College | Methods of amplifying whole genome of a single cell |
| WO2013022961A1 (en) * | 2011-08-08 | 2013-02-14 | 3The Broad Institute | Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence |
| EP2798089B1 (en) | 2011-12-30 | 2018-05-23 | Bio-rad Laboratories, Inc. | Methods and compositions for performing nucleic acid amplification reactions |
| GB2513024B (en) | 2012-02-27 | 2016-08-31 | Cellular Res Inc | A clonal amplification method |
| SG11201405669XA (en) | 2012-03-13 | 2014-10-30 | Swift Biosciences Inc | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
| HK1198836A1 (en) * | 2012-03-30 | 2015-06-12 | 深圳华大基因科技服务有限公司 | Whole genome amplification method and application thereof |
| CN104271770A (zh) * | 2012-04-30 | 2015-01-07 | 奇亚根有限公司 | 靶dna富集和测序 |
| DK3428290T3 (da) | 2012-07-26 | 2022-07-04 | Illumina Inc | Sammensætninger og fremgangsmåder til amplifikation af nukleinsyrer |
| EP2895626B1 (en) * | 2012-09-12 | 2019-12-18 | The Regents of The University of California | Accurate genome sequencing of single cells by single-stranded amplification and sequencing |
| USRE50065E1 (en) | 2012-10-17 | 2024-07-30 | 10X Genomics Sweden Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| EP2722399A1 (en) | 2012-10-18 | 2014-04-23 | Roche Diagniostics GmbH | Method for preventing high molecular weight products during amplification |
| KR101451874B1 (ko) * | 2013-02-27 | 2014-10-16 | 국민대학교산학협력단 | 실시간 pcr을 이용한 미생물의 최단시간 검출방법 |
| CN105849275B (zh) | 2013-06-25 | 2020-03-17 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
| ES2857908T3 (es) | 2013-08-28 | 2021-09-29 | Becton Dickinson Co | Análisis masivamente paralelo de células individuales |
| US10370707B2 (en) | 2013-10-09 | 2019-08-06 | Fluoresentric, Inc. | Multiplex probes |
| US20160257985A1 (en) | 2013-11-18 | 2016-09-08 | Rubicon Genomics, Inc. | Degradable adaptors for background reduction |
| US9293316B2 (en) | 2014-04-04 | 2016-03-22 | Thermo Finnigan Llc | Ion separation and storage system |
| CN105463585B (zh) * | 2014-09-12 | 2018-04-20 | 清华大学 | 基于单链dna分子构建测序文库的方法及其应用 |
| EP3208343B1 (en) * | 2014-10-13 | 2022-01-05 | MGI Tech Co., Ltd. | Nucleic acid fragmentation method and sequence combination |
| EP3208344B1 (en) * | 2014-10-17 | 2020-04-22 | MGI Tech Co., Ltd. | Primer for nucleic acid random fragmentation and nucleic acid random fragmentation method |
| WO2016138292A1 (en) * | 2015-02-25 | 2016-09-01 | Igenomx International Genomics Corporation | Methods and compositions for in silico long read sequencing |
| ES2836802T3 (es) | 2015-02-27 | 2021-06-28 | Becton Dickinson Co | Códigos de barras moleculares espacialmente direccionables |
| US10301660B2 (en) | 2015-03-30 | 2019-05-28 | Takara Bio Usa, Inc. | Methods and compositions for repair of DNA ends by multiple enzymatic activities |
| EP4180535A1 (en) | 2015-03-30 | 2023-05-17 | Becton, Dickinson and Company | Methods and compositions for combinatorial barcoding |
| WO2016156529A1 (en) * | 2015-03-31 | 2016-10-06 | Qiagen Gmbh | Efficiency improving ligation methods |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| CN114250274A (zh) * | 2015-04-24 | 2022-03-29 | 阿提拉生物系统公司 | 利用有限核苷酸组成的引物扩增 |
| US10894980B2 (en) | 2015-07-17 | 2021-01-19 | President And Fellows Of Harvard College | Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes |
| CA2994601C (en) * | 2015-08-06 | 2020-08-25 | F. Hoffmann-La Roche Ag | Target enrichment by single probe primer extension |
| CN105602939A (zh) * | 2015-09-02 | 2016-05-25 | 序康医疗科技(苏州)有限公司 | 扩增dna的方法 |
| ES2745694T3 (es) | 2015-09-11 | 2020-03-03 | Cellular Res Inc | Métodos y composiciones para normalización de biblioteca de ácido nucleico |
| CN105506125B (zh) * | 2016-01-12 | 2019-01-22 | 上海美吉生物医药科技有限公司 | 一种dna的测序方法及一种二代测序文库 |
| US11339427B2 (en) | 2016-02-12 | 2022-05-24 | Jumpcode Genomics, Inc. | Method for target specific RNA transcription of DNA sequences |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| WO2018003220A1 (ja) * | 2016-06-29 | 2018-01-04 | トヨタ自動車株式会社 | Dnaライブラリーの作製方法及びdnaライブラリーを用いたゲノムdna解析方法 |
| JP7343264B2 (ja) * | 2016-06-29 | 2023-09-12 | トヨタ自動車株式会社 | Dnaライブラリーの作製方法及びdnaライブラリーを用いたゲノムdna解析方法 |
| CN109477142B (zh) * | 2016-07-18 | 2022-03-22 | 豪夫迈·罗氏有限公司 | 不对称模板和核酸测序的不对称方法 |
| JP6882453B2 (ja) * | 2016-08-31 | 2021-06-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 全ゲノムデジタル増幅方法 |
| AU2017324946C1 (en) | 2016-09-06 | 2022-06-09 | Integrated Dna Technologies, Inc. | Normalization of NGS library concentration |
| KR102522023B1 (ko) | 2016-09-26 | 2023-04-17 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| CN109790570B (zh) | 2016-09-30 | 2023-09-15 | 富士胶片株式会社 | 获取来源于脊椎动物的单细胞的碱基序列信息的方法 |
| EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| JP7056012B2 (ja) | 2017-05-19 | 2022-04-19 | トヨタ自動車株式会社 | ランダムプライマーセット、及びこれを用いたdnaライブラリーの作製方法 |
| KR102790050B1 (ko) | 2017-06-05 | 2025-04-04 | 백톤 디킨슨 앤드 컴퍼니 | 단일 세포를 위한 샘플 인덱싱 |
| WO2019018561A1 (en) * | 2017-07-19 | 2019-01-24 | The Scripps Research Institute | GENOMIC LIBRARY GENERATION IN SOLID PHASE FOR HIGH FLOW SEQUENCING |
| CA3072591A1 (en) | 2017-08-11 | 2019-02-14 | Atila Biosystems Incorporated | Digital amplification with primers of limited nucleotide composition |
| AU2018378827B2 (en) * | 2017-12-07 | 2023-04-13 | Massachusetts Institute Of Technology | Single cell analyses |
| CN109971843B (zh) * | 2017-12-27 | 2022-11-11 | 复旦大学泰州健康科学研究院 | 一种单细胞转录组的测序方法 |
| WO2019213294A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| EP4545647A3 (en) | 2018-05-03 | 2025-07-09 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| EP4471156A3 (en) | 2018-10-01 | 2025-02-26 | Becton, Dickinson and Company | Determining 5' transcript sequences |
| JP7618548B2 (ja) | 2018-11-08 | 2025-01-21 | ベクトン・ディキンソン・アンド・カンパニー | ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析 |
| WO2020123318A1 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Resolving spatial arrays using deconvolution |
| WO2020123384A1 (en) | 2018-12-13 | 2020-06-18 | Cellular Research, Inc. | Selective extension in single cell whole transcriptome analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| ES2989967T3 (es) | 2019-01-23 | 2024-11-28 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| US12071617B2 (en) | 2019-02-14 | 2024-08-27 | Becton, Dickinson And Company | Hybrid targeted and whole transcriptome amplification |
| EP3924489A4 (en) * | 2019-02-15 | 2022-11-23 | Takara Bio USA, Inc. | METHODS FOR PREPARING AND ANALYZING NUCLEIC ACID BANKS |
| WO2020191521A1 (zh) * | 2019-03-22 | 2020-10-01 | 深圳华大智造科技有限公司 | 核酸序列、rna目标区域测序文库的构建方法及应用 |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| CN114144520B (zh) * | 2019-06-26 | 2024-12-13 | 豪夫迈·罗氏有限公司 | 通过芳香族化合物增强核酸聚合 |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| GB201911515D0 (en) * | 2019-08-12 | 2019-09-25 | Univ London Queen Mary | Methods for generating a population of polynucleotide molecules |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| CN114729350A (zh) | 2019-11-08 | 2022-07-08 | 贝克顿迪金森公司 | 使用随机引发获得用于免疫组库测序的全长v(d)j信息 |
| WO2021127406A1 (en) * | 2019-12-19 | 2021-06-24 | The Regents Of The University Of California | Methods of producing target capture nucleic acids |
| ES2982420T3 (es) | 2019-12-23 | 2024-10-16 | 10X Genomics Inc | Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| ES2965354T3 (es) | 2020-04-22 | 2024-04-12 | 10X Genomics Inc | Métodos para análisis espacial que usan eliminación de ARN elegido como diana |
| US11702689B2 (en) * | 2020-04-24 | 2023-07-18 | Microsoft Technology Licensing, Llc | Homopolymer primers for amplification of polynucleotides created by enzymatic synthesis |
| EP4150118A1 (en) | 2020-05-14 | 2023-03-22 | Becton Dickinson and Company | Primers for immune repertoire profiling |
| WO2021236929A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| WO2021237087A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| EP4158055B1 (en) | 2020-06-02 | 2024-03-27 | Becton, Dickinson and Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| WO2021263111A1 (en) | 2020-06-25 | 2021-12-30 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| AU2021409136A1 (en) | 2020-12-21 | 2023-06-29 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US11280028B1 (en) | 2021-02-24 | 2022-03-22 | Agency For Science, Technology And Research (A*Star) | Unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| ES3030033T3 (en) | 2021-06-03 | 2025-06-26 | 10X Genomics Inc | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| ES3011462T3 (en) | 2021-09-01 | 2025-04-07 | 10X Genomics Inc | Methods for blocking a capture probe on a spatial array |
| WO2023086880A1 (en) * | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| DE102022211087A1 (de) * | 2022-10-19 | 2024-04-25 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches |
| EP4628594A1 (en) * | 2024-04-05 | 2025-10-08 | 4basebio UK Ltd | Methods of amplifying dna and dna products with inhibitory regions |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6114149A (en) * | 1988-07-26 | 2000-09-05 | Genelabs Technologies, Inc. | Amplification of mixed sequence nucleic acid fragments |
| US6280949B1 (en) * | 1997-10-08 | 2001-08-28 | Yale University | Multiple displacement amplification |
| US20020045169A1 (en) * | 2000-08-25 | 2002-04-18 | Shoemaker Daniel D. | Gene discovery using microarrays |
| EP1275738A1 (en) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Method for random cDNA synthesis and amplification |
Family Cites Families (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| AU622104B2 (en) | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
| ATE92538T1 (de) | 1988-01-21 | 1993-08-15 | Genentech Inc | Verstaerkung und nachweis von nukleinsaeuresequenzen. |
| US6107023A (en) | 1988-06-17 | 2000-08-22 | Genelabs Technologies, Inc. | DNA amplification and subtraction techniques |
| US4932207A (en) | 1988-12-28 | 1990-06-12 | Sundstrand Corporation | Segmented seal plate for a turbine engine |
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5106727A (en) | 1989-04-27 | 1992-04-21 | Life Technologies, Inc. | Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers |
| US6040138A (en) * | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
| US5104792A (en) | 1989-12-21 | 1992-04-14 | The United States Of America As Represented By The Department Of Health And Human Services | Method for amplifying unknown nucleic acid sequences |
| RU2182176C2 (ru) | 1991-09-24 | 2002-05-10 | Кейгене Н.В. | Способ селективной амплификации, олигонуклеотид и набор для селективной амплификации |
| WO1993014217A1 (en) * | 1992-01-10 | 1993-07-22 | Life Technologies, Inc. | Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules |
| CA2135073C (en) * | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| US5514545A (en) | 1992-06-11 | 1996-05-07 | Trustees Of The University Of Pennsylvania | Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics |
| US5731171A (en) * | 1993-07-23 | 1998-03-24 | Arch Development Corp. | Sequence independent amplification of DNA |
| US5523204A (en) * | 1993-12-10 | 1996-06-04 | Becton Dickinson And Company | Detection of nucleic acids in cells by strand displacement amplification |
| US5710000A (en) | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| GB9422891D0 (en) | 1994-11-12 | 1995-01-04 | Univ Nottingham | Improvements in and relating to the amplification and identification of nucleotide sequences |
| JPH08173164A (ja) | 1994-12-22 | 1996-07-09 | Hitachi Ltd | Dna調製法 |
| US5565340A (en) | 1995-01-27 | 1996-10-15 | Clontech Laboratories, Inc. | Method for suppressing DNA fragment amplification during PCR |
| US5994058A (en) | 1995-03-20 | 1999-11-30 | Genome International Corporation | Method for contiguous genome sequencing |
| US5871820A (en) | 1995-04-06 | 1999-02-16 | General Electric Company | Protection of thermal barrier coating with an impermeable barrier coating |
| US5750341A (en) * | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5648245A (en) | 1995-05-09 | 1997-07-15 | Carnegie Institution Of Washington | Method for constructing an oligonucleotide concatamer library by rolling circle replication |
| US5814444A (en) | 1995-06-07 | 1998-09-29 | University Of Washington | Methods for making and using single-chromosome amplfication libraries |
| US5631147A (en) * | 1995-09-21 | 1997-05-20 | Becton, Dickinson And Company | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
| EP0880598A4 (en) * | 1996-01-23 | 2005-02-23 | Affymetrix Inc | RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM |
| EP0880537B1 (en) | 1996-02-14 | 2004-12-15 | bioMerieux B.V. | Isolation of single stranded nucleic acids |
| AU3116997A (en) * | 1996-05-02 | 1997-11-19 | Immunological Associates Of Denver | Quantitation of rna transcripts using genomic dna as the internal amplification competitor |
| WO1998002575A1 (en) | 1996-07-16 | 1998-01-22 | Periannan Senapathy | Method for contiguous genome sequencing |
| CA2744096C (en) | 1996-07-31 | 2013-07-30 | Laboratory Corporation Of America Holdings | Biomarkers and targets for diagnosis, prognosis and management of prostate disease |
| EP0835935A1 (en) * | 1996-10-03 | 1998-04-15 | Roche Diagnostics GmbH | Thermostable DNA Polymerase from Anaerocellum thermophilum |
| US6030814A (en) * | 1997-04-21 | 2000-02-29 | Epicentre Technologies Corporation | Reverse transcription method |
| US5932451A (en) | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
| DE19813317A1 (de) | 1998-03-26 | 1999-09-30 | Roche Diagnostics Gmbh | Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR |
| US6232067B1 (en) * | 1998-08-17 | 2001-05-15 | The Perkin-Elmer Corporation | Adapter directed expression analysis |
| AU755499B2 (en) | 1998-09-18 | 2002-12-12 | Micromet Ag | DNA amplification of a single cell |
| US6506594B1 (en) * | 1999-03-19 | 2003-01-14 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US6521428B1 (en) | 1999-04-21 | 2003-02-18 | Genome Technologies, Llc | Shot-gun sequencing and amplification without cloning |
| US6491752B1 (en) | 1999-07-16 | 2002-12-10 | Sumco Oregon Corporation | Enhanced n-type silicon material for epitaxial wafer substrate and method of making same |
| AU6387000A (en) | 1999-07-29 | 2001-02-19 | Genzyme Corporation | Serial analysis of genetic alterations |
| US6846626B1 (en) * | 1999-09-01 | 2005-01-25 | Genome Technologies, Llc | Method for amplifying sequences from unknown DNA |
| US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| US6271002B1 (en) | 1999-10-04 | 2001-08-07 | Rosetta Inpharmatics, Inc. | RNA amplification method |
| US6958225B2 (en) | 1999-10-27 | 2005-10-25 | Affymetrix, Inc. | Complexity management of genomic DNA |
| AU2001232485A1 (en) | 2000-01-13 | 2001-07-24 | Amsterdam Support Diagnostics B.V. | A universal nucleic acid amplification system for nucleic acids in a sample |
| US6379932B1 (en) | 2000-07-17 | 2002-04-30 | Incyte Genomics, Inc. | Single primer PCR amplification of RNA |
| US7264931B2 (en) * | 2000-09-06 | 2007-09-04 | Rutgers, The State University Of New Jersey | Method for sequencing nucleic acid sequences without chain termination |
| WO2002020571A2 (en) | 2000-09-08 | 2002-03-14 | Biomerieux B.V.A. | Attenuated hiv strains and use thereof |
| US6794141B2 (en) | 2000-12-22 | 2004-09-21 | Arcturus Bioscience, Inc. | Nucleic acid amplification |
| WO2002061140A2 (en) * | 2001-01-31 | 2002-08-08 | Ambion, Inc. | Competitive population normalization for comparative analysis of nucleic acid samples |
| CA2437737A1 (en) | 2001-02-14 | 2002-08-22 | Stephen D. Ginsberg | Methods and compositions of amplifying rna |
| BR0205268A (pt) | 2001-03-09 | 2004-11-30 | Nugen Technologies Inc | Processos e composições para a mplificação de sequências de rna |
| WO2002103054A1 (en) | 2001-05-02 | 2002-12-27 | Rubicon Genomics Inc. | Genome walking by selective amplification of nick-translate dna library and amplification from complex mixtures of templates |
| JP2002345466A (ja) | 2001-05-08 | 2002-12-03 | Takara Bio Inc | Dnaの増幅方法 |
| US6638722B2 (en) | 2001-06-13 | 2003-10-28 | Invitrogen Corporation | Method for rapid amplification of DNA |
| EP1275734A1 (en) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Method for random cDNA synthesis and amplification |
| US20040175715A1 (en) | 2001-08-21 | 2004-09-09 | Burgoyne Leigh A. | Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genomes mixtures |
| US20040019005A1 (en) * | 2001-10-01 | 2004-01-29 | Jeffrey Van Ness | Methods for parallel measurement of genetic variations |
| JP2005535283A (ja) | 2001-11-13 | 2005-11-24 | ルビコン ゲノミクス インコーポレイテッド | ランダムフラグメント化により生成されたdna分子を用いたdna増幅および配列決定 |
| US20030211528A1 (en) * | 2002-03-12 | 2003-11-13 | Norman Iscove | Exponential amplification of sub-picogram nucleic acid samples with retention of quantitative representation |
| KR100545295B1 (ko) * | 2003-09-29 | 2006-01-24 | 현대모비스 주식회사 | 차량용 조향장치의 너클 어셈블리 |
| CA2902980A1 (en) | 2004-03-08 | 2005-09-29 | Rubicon Genomics, Inc. | Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation |
| US8312249B1 (en) | 2008-10-10 | 2012-11-13 | Apple Inc. | Dynamic trampoline and structured code generation in a signed code environment |
| FR2957821B1 (fr) | 2010-03-24 | 2014-08-29 | Inst Francais Du Petrole | Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives |
-
2004
- 2004-03-08 DE DE602004029560T patent/DE602004029560D1/de not_active Expired - Lifetime
- 2004-03-08 DK DK10011482.6T patent/DK2374900T3/en active
- 2004-03-08 DK DK04718499.9T patent/DK1604040T3/da active
- 2004-03-08 EP EP10011482.6A patent/EP2374900B1/en not_active Expired - Lifetime
- 2004-03-08 WO PCT/US2004/006983 patent/WO2004081225A2/en not_active Ceased
- 2004-03-08 US US10/795,667 patent/US7718403B2/en active Active
- 2004-03-08 EP EP04718499A patent/EP1604040B1/en not_active Expired - Lifetime
- 2004-03-08 JP JP2006509236A patent/JP4773338B2/ja not_active Expired - Lifetime
- 2004-03-08 AT AT04718499T patent/ATE484598T1/de not_active IP Right Cessation
-
2006
- 2006-11-06 US US11/593,625 patent/US20070054311A1/en not_active Abandoned
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6114149A (en) * | 1988-07-26 | 2000-09-05 | Genelabs Technologies, Inc. | Amplification of mixed sequence nucleic acid fragments |
| US6280949B1 (en) * | 1997-10-08 | 2001-08-28 | Yale University | Multiple displacement amplification |
| US20020045169A1 (en) * | 2000-08-25 | 2002-04-18 | Shoemaker Daniel D. | Gene discovery using microarrays |
| EP1275738A1 (en) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Method for random cDNA synthesis and amplification |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1604040B1 (en) | 2010-10-13 |
| DE602004029560D1 (de) | 2010-11-25 |
| US7718403B2 (en) | 2010-05-18 |
| HK1089485A1 (en) | 2006-12-01 |
| JP2006519621A (ja) | 2006-08-31 |
| EP2374900B1 (en) | 2016-07-13 |
| EP1604040A2 (en) | 2005-12-14 |
| ATE484598T1 (de) | 2010-10-15 |
| US20070054311A1 (en) | 2007-03-08 |
| DK1604040T3 (da) | 2011-01-24 |
| WO2004081225A3 (en) | 2004-11-25 |
| DK2374900T3 (en) | 2016-10-17 |
| US20040209298A1 (en) | 2004-10-21 |
| WO2004081225A2 (en) | 2004-09-23 |
| EP2374900A1 (en) | 2011-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4773338B2 (ja) | Dna重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析 | |
| US11492663B2 (en) | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process | |
| EP3538662B1 (en) | Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein | |
| US8034568B2 (en) | Isothermal nucleic acid amplification methods and compositions | |
| US10988795B2 (en) | Synthesis of double-stranded nucleic acids | |
| JP2020522243A (ja) | 核酸のマルチプレックス末端タギング増幅 | |
| CN112689673A (zh) | 转座体使能的dna/rna测序(ted rna-seq) | |
| US20090053775A1 (en) | Copy dna and sense rna | |
| WO2008013010A1 (fr) | Procédé d'amplification d'une séquence nucléotidique | |
| GB2533882A (en) | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation | |
| CN114555802A (zh) | 单细胞分析 | |
| EP3330386A1 (en) | Preparation of adapter-ligated amplicons | |
| WO2021077415A1 (en) | Methylation detection and analysis of mammalian dna | |
| WO2021156295A1 (en) | Methods for amplification of genomic dna and preparation of sequencing libraries | |
| HK1089485B (en) | Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070214 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070214 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20091202 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20100226 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20100305 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100524 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20101118 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110218 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20110525 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20110623 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140701 Year of fee payment: 3 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 4773338 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313111 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |