JP2013507603A - 署名ペプチドおよび質量分析による混合物中の個々の組換えタンパク質の多重定量化 - Google Patents

署名ペプチドおよび質量分析による混合物中の個々の組換えタンパク質の多重定量化 Download PDF

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JP2013507603A
JP2013507603A JP2012532458A JP2012532458A JP2013507603A JP 2013507603 A JP2013507603 A JP 2013507603A JP 2012532458 A JP2012532458 A JP 2012532458A JP 2012532458 A JP2012532458 A JP 2012532458A JP 2013507603 A JP2013507603 A JP 2013507603A
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recombinant
peptide
polyclonal antibody
antibody
recombinant polyclonal
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JP2013507603A5 (enExample
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トーベン・フランセン
ヘンリク・ネイスト
イェット・バウトベアウ・セン
ペルニル・フォウ・イェンセン
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Symphogen AS
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • G01N33/6851Methods of protein analysis involving laser desorption ionisation mass spectrometry

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
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  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2012532458A 2009-10-09 2010-10-07 署名ペプチドおよび質量分析による混合物中の個々の組換えタンパク質の多重定量化 Pending JP2013507603A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200901112 2009-10-09
DKPA200901112 2009-10-09
US25623209P 2009-10-29 2009-10-29
US61/256,232 2009-10-29
PCT/DK2010/050258 WO2011042027A2 (en) 2009-10-09 2010-10-07 Multiplex quantitation of individual recombinant proteins in a mixture by signature peptides and mass spectrometry

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JP2013507603A true JP2013507603A (ja) 2013-03-04
JP2013507603A5 JP2013507603A5 (enExample) 2013-11-21

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JP2012532458A Pending JP2013507603A (ja) 2009-10-09 2010-10-07 署名ペプチドおよび質量分析による混合物中の個々の組換えタンパク質の多重定量化

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US (1) US20120264155A1 (enExample)
EP (1) EP2486411A2 (enExample)
JP (1) JP2013507603A (enExample)
KR (1) KR20120100982A (enExample)
CN (1) CN102687020A (enExample)
AU (1) AU2010305150A1 (enExample)
BR (1) BR112012007815A2 (enExample)
CA (1) CA2776508A1 (enExample)
IL (1) IL218821A0 (enExample)
IN (1) IN2012DN03911A (enExample)
MX (1) MX2012003538A (enExample)
RU (1) RU2012118620A (enExample)
WO (1) WO2011042027A2 (enExample)
ZA (1) ZA201202431B (enExample)

Cited By (6)

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JP2019518195A (ja) * 2016-03-25 2019-06-27 ジェネンテック, インコーポレイテッド 多重全抗体及び抗体複合体化薬物定量化アッセイ
JP2019196989A (ja) * 2018-05-10 2019-11-14 株式会社島津製作所 分析方法、処理装置、分析装置およびプログラム
JP2020530103A (ja) * 2017-08-01 2020-10-15 アムジエン・インコーポレーテツド 質量分析法による分析用ポリペプチド試料のリアルタイム調製のためのシステム及び方法
JP2021512280A (ja) * 2018-01-31 2021-05-13 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 治療用モノクローナル抗体に対する酸性翻訳後修飾としてのグルクロニル化
JP2022069446A (ja) * 2016-02-25 2022-05-11 ザ バインディング サイト グループ リミティド 質量分析キット
JP2022542376A (ja) * 2019-07-26 2022-10-03 ザ バインディング サイト グループ リミティド イオン化コントロール

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EP2241875A1 (en) * 2009-04-14 2010-10-20 Koninklijke Philips Electronics N.V. Up-concentration of organic microobjects for microscopic imaging.
CA2833212C (en) 2011-05-12 2020-06-09 Genentech, Inc. Multiple reaction monitoring lc-ms/ms method to detect therapeutic antibodies in animal samples using framework signature peptides
WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
WO2016022696A1 (en) * 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
EP3268746B1 (en) 2015-03-12 2024-07-17 Mars, Incorporated Ultra high resolution mass spectrometry and methods of using the same
WO2016179397A1 (en) * 2015-05-06 2016-11-10 Momenta Pharmaceuticals, Inc. Methods of glycoprotein analysis
CN105242050A (zh) * 2015-09-16 2016-01-13 同济大学 一种不同生理或病理条件下整体蛋白质的定量分析方法
CN105218671A (zh) * 2015-09-29 2016-01-06 李艳华 一种传染病病原体全人源化抗体的制备方法
WO2017205741A1 (en) 2016-05-27 2017-11-30 Genentech, Inc. Bioanalytical method for the characterization of site-specific antibody-drug conjugates
CN107525934A (zh) * 2016-06-21 2017-12-29 张效龙 一种测定血液中抗体氨基酸序列的方法
US20210333288A1 (en) * 2016-08-17 2021-10-28 Momenta Pharmaceuticals, Inc. Glycan oxonium ion profiling of glycosylated proteins
CN108072728A (zh) * 2016-11-16 2018-05-25 中国科学院大连化学物理研究所 一种基于数据依赖性质谱扫描模式的谱图库建立方法及其应用
CN108956789A (zh) * 2017-05-17 2018-12-07 中国科学院大连化学物理研究所 一种血清中免疫球蛋白g糖肽的绝对定量分析方法
US10615016B2 (en) * 2017-09-07 2020-04-07 Thermo Fisher Scientific (Bremen) Gmbh Determining isotope ratios using mass spectrometry
CN108226516B (zh) * 2017-11-30 2020-05-19 中国农业科学院北京畜牧兽医研究所 一种相对定量分析肉鸡脂肪酸去饱和酶fads1的方法
CN108169397B (zh) * 2017-11-30 2019-11-15 中国农业科学院北京畜牧兽医研究所 一种相对定量分析肉鸡谷胱甘肽s转移酶gsta2的方法
CN109900815B (zh) * 2017-12-08 2021-06-29 中国科学院大连化学物理研究所 血清中IgG糖肽的绝对定量分析
CN110824096B (zh) * 2018-08-13 2022-11-29 齐鲁制药有限公司 一种蛋白错配二硫键的分析方法
CN110007019A (zh) * 2019-03-29 2019-07-12 绿城农科检测技术有限公司 一种同时定量检测母乳中三种抗体含量的方法
JP7778705B2 (ja) 2020-02-21 2025-12-02 ジェンマブ ビー.ブイ. 糖蛋白質の定量のための方法
CN111551739A (zh) * 2020-04-27 2020-08-18 杭州璞湃科技有限公司 一种免疫球蛋白a和免疫球蛋白a1的检测方法及其试剂盒
US20230228763A1 (en) * 2020-06-18 2023-07-20 Northwestern University Process for direct readout of immunoglobulins
JP7478040B2 (ja) * 2020-06-25 2024-05-02 シスメックス株式会社 Aβペプチドの測定方法及びその方法に用いられる試薬組成物
CN111766323B (zh) * 2020-07-10 2022-06-14 中国检验检疫科学研究院 一种用于检测骆驼奶中掺入牛奶的特征肽组合及方法
CN113933409B (zh) * 2021-09-14 2023-11-14 上海中科新生命生物科技有限公司 基于液相色谱-质谱检测血清中特瑞普利单抗的方法
DE102022115561B4 (de) * 2022-06-22 2024-06-13 Bruker Daltonics GmbH & Co. KG Massenspektrometrische Bestimmung der Zelltoxizität
CN118221778A (zh) * 2024-04-25 2024-06-21 烟台大学 拟穴青蟹Scyreprocin蛋白的特征肽段及其在雌雄鉴别方面的应用
CN120399082B (zh) * 2025-07-04 2025-08-26 北京溯本源和生物科技有限公司 一种用于检测猫Cys-C蛋白的单克隆抗体组合及应用

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US20040033530A1 (en) * 2002-04-08 2004-02-19 Awrey Donald E. High throughput purification, characterization and identification of recombinant proteins
WO2006080396A1 (ja) * 2005-01-28 2006-08-03 Shionogi & Co., Ltd. 組換え蛋白質の定量法
JP2006300758A (ja) * 2005-04-21 2006-11-02 Apro Life Science Institute Inc 内部標準ペプチドを用いて生体由来試料に含まれる標的タンパク質を定量する方法
WO2008106980A2 (en) * 2007-03-06 2008-09-12 Symphogen A/S Recombinant antibodies for treatment of respiratory syncytial virus infections

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WO2006080396A1 (ja) * 2005-01-28 2006-08-03 Shionogi & Co., Ltd. 組換え蛋白質の定量法
JP2006300758A (ja) * 2005-04-21 2006-11-02 Apro Life Science Institute Inc 内部標準ペプチドを用いて生体由来試料に含まれる標的タンパク質を定量する方法
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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022069446A (ja) * 2016-02-25 2022-05-11 ザ バインディング サイト グループ リミティド 質量分析キット
JP7541045B2 (ja) 2016-02-25 2024-08-27 ザ バインディング サイト グループ リミティド 質量分析キット
JP2019518195A (ja) * 2016-03-25 2019-06-27 ジェネンテック, インコーポレイテッド 多重全抗体及び抗体複合体化薬物定量化アッセイ
JP2020530103A (ja) * 2017-08-01 2020-10-15 アムジエン・インコーポレーテツド 質量分析法による分析用ポリペプチド試料のリアルタイム調製のためのシステム及び方法
JP7237022B2 (ja) 2017-08-01 2023-03-10 アムジエン・インコーポレーテツド 質量分析法による分析用ポリペプチド試料のリアルタイム調製のためのシステム及び方法
JP2021512280A (ja) * 2018-01-31 2021-05-13 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 治療用モノクローナル抗体に対する酸性翻訳後修飾としてのグルクロニル化
JP7301054B2 (ja) 2018-01-31 2023-06-30 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 治療用モノクローナル抗体に対する酸性翻訳後修飾としてのグルクロニル化
JP2019196989A (ja) * 2018-05-10 2019-11-14 株式会社島津製作所 分析方法、処理装置、分析装置およびプログラム
JP7205077B2 (ja) 2018-05-10 2023-01-17 株式会社島津製作所 分析方法、処理装置、分析装置およびプログラム
JP2022542376A (ja) * 2019-07-26 2022-10-03 ザ バインディング サイト グループ リミティド イオン化コントロール
JP7738546B2 (ja) 2019-07-26 2025-09-12 ザ バインディング サイト グループ リミティド イオン化コントロール

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Publication number Publication date
RU2012118620A (ru) 2013-11-20
WO2011042027A3 (en) 2011-09-22
EP2486411A2 (en) 2012-08-15
KR20120100982A (ko) 2012-09-12
IL218821A0 (en) 2012-06-28
CA2776508A1 (en) 2011-04-14
BR112012007815A2 (pt) 2018-03-20
IN2012DN03911A (enExample) 2015-09-04
US20120264155A1 (en) 2012-10-18
MX2012003538A (es) 2012-08-03
AU2010305150A1 (en) 2012-04-05
WO2011042027A2 (en) 2011-04-14
CN102687020A (zh) 2012-09-19
ZA201202431B (en) 2012-12-27

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