MX2012003538A - Cuantificacion multiple de proteinas recombinantes individuales en una mezcla mediante peptidos distintivos y espectrometria de masas. - Google Patents
Cuantificacion multiple de proteinas recombinantes individuales en una mezcla mediante peptidos distintivos y espectrometria de masas.Info
- Publication number
- MX2012003538A MX2012003538A MX2012003538A MX2012003538A MX2012003538A MX 2012003538 A MX2012003538 A MX 2012003538A MX 2012003538 A MX2012003538 A MX 2012003538A MX 2012003538 A MX2012003538 A MX 2012003538A MX 2012003538 A MX2012003538 A MX 2012003538A
- Authority
- MX
- Mexico
- Prior art keywords
- mixture
- recombinant proteins
- mass spectrometry
- signature peptides
- individual recombinant
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
- G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Optics & Photonics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Peptides Or Proteins (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La presente invención se relaciona con un método analítico para la cuantificación de proteínas recombinantes múltiples seleccionadas en una matriz compleja tal como anticuerpos policlonales recombinantes en suero o anticuerpos policlonales recombinantes expresados en un sobrenadante de cultivo.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200901112 | 2009-10-09 | ||
US25623209P | 2009-10-29 | 2009-10-29 | |
PCT/DK2010/050258 WO2011042027A2 (en) | 2009-10-09 | 2010-10-07 | Multiplex quantitation of individual recombinant proteins in a mixture by signature peptides and mass spectrometry |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2012003538A true MX2012003538A (es) | 2012-08-03 |
Family
ID=42166454
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2012003538A MX2012003538A (es) | 2009-10-09 | 2010-10-07 | Cuantificacion multiple de proteinas recombinantes individuales en una mezcla mediante peptidos distintivos y espectrometria de masas. |
Country Status (14)
Country | Link |
---|---|
US (1) | US20120264155A1 (es) |
EP (1) | EP2486411A2 (es) |
JP (1) | JP2013507603A (es) |
KR (1) | KR20120100982A (es) |
CN (1) | CN102687020A (es) |
AU (1) | AU2010305150A1 (es) |
BR (1) | BR112012007815A2 (es) |
CA (1) | CA2776508A1 (es) |
IL (1) | IL218821A0 (es) |
IN (1) | IN2012DN03911A (es) |
MX (1) | MX2012003538A (es) |
RU (1) | RU2012118620A (es) |
WO (1) | WO2011042027A2 (es) |
ZA (1) | ZA201202431B (es) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
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EP2241875A1 (en) * | 2009-04-14 | 2010-10-20 | Koninklijke Philips Electronics N.V. | Up-concentration of organic microobjects for microscopic imaging. |
MX2013013054A (es) * | 2011-05-12 | 2014-02-20 | Genentech Inc | Metodo de monitoreo de lc-ms/ms de reaccion multiple para detectar anticuerpos terapeuticos en muestras de animales utilizando peptidos de firma de estructura. |
WO2013019714A1 (en) | 2011-07-29 | 2013-02-07 | The Trustees Of Columbia University In The City Of New York | Mems affinity sensor for continuous monitoring of analytes |
WO2016022696A1 (en) | 2014-08-05 | 2016-02-11 | The Trustees Of Columbia University In The City Of New York | Method of isolating aptamers for minimal residual disease detection |
WO2016145390A1 (en) | 2015-03-12 | 2016-09-15 | Mars, Incorporated | Ultra high resolution mass spectrometry and methods of using the same |
US10794916B2 (en) | 2015-05-06 | 2020-10-06 | Momenta Pharmaceuticals, Inc. | Methods of glycoprotein analysis |
CN105242050A (zh) * | 2015-09-16 | 2016-01-13 | 同济大学 | 一种不同生理或病理条件下整体蛋白质的定量分析方法 |
CN105218671A (zh) * | 2015-09-29 | 2016-01-06 | 李艳华 | 一种传染病病原体全人源化抗体的制备方法 |
GB201603291D0 (en) * | 2016-02-25 | 2016-04-13 | Binding Site Group The Ltd | Antibodies |
US20170315132A1 (en) * | 2016-03-25 | 2017-11-02 | Genentech, Inc. | Multiplexed total antibody and antibody-conjugated drug quantification assay |
WO2017205741A1 (en) | 2016-05-27 | 2017-11-30 | Genentech, Inc. | Bioanalytical method for the characterization of site-specific antibody-drug conjugates |
CN107525934A (zh) * | 2016-06-21 | 2017-12-29 | 张效龙 | 一种测定血液中抗体氨基酸序列的方法 |
WO2018035350A1 (en) * | 2016-08-17 | 2018-02-22 | Momenta Pharmaceuticals, Inc. | Glycan oxonium ion profiling of glycosylated proteins |
CN108072728A (zh) * | 2016-11-16 | 2018-05-25 | 中国科学院大连化学物理研究所 | 一种基于数据依赖性质谱扫描模式的谱图库建立方法及其应用 |
CN108956789A (zh) * | 2017-05-17 | 2018-12-07 | 中国科学院大连化学物理研究所 | 一种血清中免疫球蛋白g糖肽的绝对定量分析方法 |
EA202090399A1 (ru) * | 2017-08-01 | 2020-05-20 | Эмджен Инк. | Системы и способы для подготовки в режиме реального времени образца полипептида для анализа с помощью масс-спектрометрии |
US10615016B2 (en) * | 2017-09-07 | 2020-04-07 | Thermo Fisher Scientific (Bremen) Gmbh | Determining isotope ratios using mass spectrometry |
CN108226516B (zh) * | 2017-11-30 | 2020-05-19 | 中国农业科学院北京畜牧兽医研究所 | 一种相对定量分析肉鸡脂肪酸去饱和酶fads1的方法 |
CN108169397B (zh) * | 2017-11-30 | 2019-11-15 | 中国农业科学院北京畜牧兽医研究所 | 一种相对定量分析肉鸡谷胱甘肽s转移酶gsta2的方法 |
CN109900815B (zh) * | 2017-12-08 | 2021-06-29 | 中国科学院大连化学物理研究所 | 血清中IgG糖肽的绝对定量分析 |
WO2019152356A2 (en) * | 2018-01-31 | 2019-08-08 | Regeneron Pharmaceuticals, Inc. | Glucuronylation as a new acidic post-translational modification on therapeutic monoclonal antibodies |
JP7205077B2 (ja) * | 2018-05-10 | 2023-01-17 | 株式会社島津製作所 | 分析方法、処理装置、分析装置およびプログラム |
CN110824096B (zh) * | 2018-08-13 | 2022-11-29 | 齐鲁制药有限公司 | 一种蛋白错配二硫键的分析方法 |
CN110007019A (zh) * | 2019-03-29 | 2019-07-12 | 绿城农科检测技术有限公司 | 一种同时定量检测母乳中三种抗体含量的方法 |
CN115427814A (zh) | 2020-02-21 | 2022-12-02 | 根马布有限公司 | 用于定量糖蛋白的方法 |
CN111551739A (zh) * | 2020-04-27 | 2020-08-18 | 杭州璞湃科技有限公司 | 一种免疫球蛋白a和免疫球蛋白a1的检测方法及其试剂盒 |
WO2021258114A1 (en) * | 2020-06-18 | 2021-12-23 | Northwestern University | Process for direct readout of immunoglobulins |
CN111766323B (zh) * | 2020-07-10 | 2022-06-14 | 中国检验检疫科学研究院 | 一种用于检测骆驼奶中掺入牛奶的特征肽组合及方法 |
CN113933409B (zh) * | 2021-09-14 | 2023-11-14 | 上海中科新生命生物科技有限公司 | 基于液相色谱-质谱检测血清中特瑞普利单抗的方法 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003087834A2 (en) * | 2002-04-08 | 2003-10-23 | Affinium Pharmaceuticals, Inc. | High throughput analysis of recombinant proteins in multi-well plates |
EP1556478B1 (en) | 2002-10-03 | 2018-07-18 | Norman Leigh Anderson | High sensitivity quantitation of peptides by mass spectrometry |
WO2005124341A2 (en) * | 2004-06-09 | 2005-12-29 | Anderson Forschung Group Llc | Stable isotope labeled polypeptide standards for protein quantitation |
EP1967529A1 (en) | 2004-07-20 | 2008-09-10 | Symphogen A/S | Anti-rhesus D recombinant polyclonal antibody and methods of manufacture |
DK1787126T3 (da) * | 2004-07-20 | 2009-12-21 | Symphogen As | Fremgangsmåde til karakterisering af en polyklonal cellelinie |
US20090298196A1 (en) * | 2005-01-28 | 2009-12-03 | Uwabe Ken-Ichiro | Quantitative measurement method for recombinant protein |
JP2006300758A (ja) * | 2005-04-21 | 2006-11-02 | Apro Life Science Institute Inc | 内部標準ペプチドを用いて生体由来試料に含まれる標的タンパク質を定量する方法 |
US7850965B2 (en) | 2005-12-05 | 2010-12-14 | Symphogen A/S | Anti-orthopoxvirus recombinant polyclonal antibody |
MX2008011280A (es) | 2006-03-06 | 2008-09-12 | Symphogen As | Anticuerpor policlonal recombinante para el tratamiento de infecciones por el virus sincitial respiratorio. |
NZ597466A (en) * | 2007-03-01 | 2013-08-30 | Symphogen As | Recombinant anti-epidermal growth factor receptor antibody compositions |
CN101679513A (zh) * | 2007-03-06 | 2010-03-24 | 西福根有限公司 | 用于治疗呼吸道合胞病毒感染的重组抗体 |
US20090000419A1 (en) * | 2007-06-29 | 2009-01-01 | Harley-Davidson Motor Company Group, Inc. | Handlebar assembly with axial locking feature |
-
2010
- 2010-10-07 CA CA2776508A patent/CA2776508A1/en not_active Abandoned
- 2010-10-07 BR BR112012007815A patent/BR112012007815A2/pt not_active IP Right Cessation
- 2010-10-07 RU RU2012118620/15A patent/RU2012118620A/ru unknown
- 2010-10-07 EP EP10774121A patent/EP2486411A2/en not_active Withdrawn
- 2010-10-07 IN IN3911DEN2012 patent/IN2012DN03911A/en unknown
- 2010-10-07 WO PCT/DK2010/050258 patent/WO2011042027A2/en active Application Filing
- 2010-10-07 AU AU2010305150A patent/AU2010305150A1/en not_active Abandoned
- 2010-10-07 JP JP2012532458A patent/JP2013507603A/ja active Pending
- 2010-10-07 MX MX2012003538A patent/MX2012003538A/es not_active Application Discontinuation
- 2010-10-07 CN CN2010800562278A patent/CN102687020A/zh active Pending
- 2010-10-07 US US13/501,051 patent/US20120264155A1/en not_active Abandoned
- 2010-10-07 KR KR1020127011934A patent/KR20120100982A/ko not_active Application Discontinuation
-
2012
- 2012-03-25 IL IL218821A patent/IL218821A0/en unknown
- 2012-04-03 ZA ZA2012/02431A patent/ZA201202431B/en unknown
Also Published As
Publication number | Publication date |
---|---|
IL218821A0 (en) | 2012-06-28 |
EP2486411A2 (en) | 2012-08-15 |
CA2776508A1 (en) | 2011-04-14 |
JP2013507603A (ja) | 2013-03-04 |
RU2012118620A (ru) | 2013-11-20 |
BR112012007815A2 (pt) | 2018-03-20 |
ZA201202431B (en) | 2012-12-27 |
KR20120100982A (ko) | 2012-09-12 |
CN102687020A (zh) | 2012-09-19 |
AU2010305150A1 (en) | 2012-04-05 |
WO2011042027A3 (en) | 2011-09-22 |
US20120264155A1 (en) | 2012-10-18 |
IN2012DN03911A (es) | 2015-09-04 |
WO2011042027A2 (en) | 2011-04-14 |
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FA | Abandonment or withdrawal |