MX2012003538A - Cuantificacion multiple de proteinas recombinantes individuales en una mezcla mediante peptidos distintivos y espectrometria de masas. - Google Patents

Cuantificacion multiple de proteinas recombinantes individuales en una mezcla mediante peptidos distintivos y espectrometria de masas.

Info

Publication number
MX2012003538A
MX2012003538A MX2012003538A MX2012003538A MX2012003538A MX 2012003538 A MX2012003538 A MX 2012003538A MX 2012003538 A MX2012003538 A MX 2012003538A MX 2012003538 A MX2012003538 A MX 2012003538A MX 2012003538 A MX2012003538 A MX 2012003538A
Authority
MX
Mexico
Prior art keywords
mixture
recombinant proteins
mass spectrometry
signature peptides
individual recombinant
Prior art date
Application number
MX2012003538A
Other languages
English (en)
Inventor
Torben Frandsen
Henrik Naested
Jette Sen Wagtberg
Pernille Foged Jensen
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen As filed Critical Symphogen As
Publication of MX2012003538A publication Critical patent/MX2012003538A/es

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Organic Chemistry (AREA)
  • Electrochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La presente invención se relaciona con un método analítico para la cuantificación de proteínas recombinantes múltiples seleccionadas en una matriz compleja tal como anticuerpos policlonales recombinantes en suero o anticuerpos policlonales recombinantes expresados en un sobrenadante de cultivo.
MX2012003538A 2009-10-09 2010-10-07 Cuantificacion multiple de proteinas recombinantes individuales en una mezcla mediante peptidos distintivos y espectrometria de masas. MX2012003538A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200901112 2009-10-09
US25623209P 2009-10-29 2009-10-29
PCT/DK2010/050258 WO2011042027A2 (en) 2009-10-09 2010-10-07 Multiplex quantitation of individual recombinant proteins in a mixture by signature peptides and mass spectrometry

Publications (1)

Publication Number Publication Date
MX2012003538A true MX2012003538A (es) 2012-08-03

Family

ID=42166454

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012003538A MX2012003538A (es) 2009-10-09 2010-10-07 Cuantificacion multiple de proteinas recombinantes individuales en una mezcla mediante peptidos distintivos y espectrometria de masas.

Country Status (14)

Country Link
US (1) US20120264155A1 (es)
EP (1) EP2486411A2 (es)
JP (1) JP2013507603A (es)
KR (1) KR20120100982A (es)
CN (1) CN102687020A (es)
AU (1) AU2010305150A1 (es)
BR (1) BR112012007815A2 (es)
CA (1) CA2776508A1 (es)
IL (1) IL218821A0 (es)
IN (1) IN2012DN03911A (es)
MX (1) MX2012003538A (es)
RU (1) RU2012118620A (es)
WO (1) WO2011042027A2 (es)
ZA (1) ZA201202431B (es)

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CA2833212C (en) * 2011-05-12 2020-06-09 Genentech, Inc. Multiple reaction monitoring lc-ms/ms method to detect therapeutic antibodies in animal samples using framework signature peptides
WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
WO2016022696A1 (en) 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
WO2016145390A1 (en) 2015-03-12 2016-09-15 Mars, Incorporated Ultra high resolution mass spectrometry and methods of using the same
WO2016179397A1 (en) * 2015-05-06 2016-11-10 Momenta Pharmaceuticals, Inc. Methods of glycoprotein analysis
CN105242050A (zh) * 2015-09-16 2016-01-13 同济大学 一种不同生理或病理条件下整体蛋白质的定量分析方法
CN105218671A (zh) * 2015-09-29 2016-01-06 李艳华 一种传染病病原体全人源化抗体的制备方法
EP4273551A3 (en) * 2016-03-25 2024-01-17 F. Hoffmann-La Roche AG Multiplexed total antibody and antibody-conjugated drug quantification assay
WO2017205741A1 (en) 2016-05-27 2017-11-30 Genentech, Inc. Bioanalytical method for the characterization of site-specific antibody-drug conjugates
CN107525934A (zh) * 2016-06-21 2017-12-29 张效龙 一种测定血液中抗体氨基酸序列的方法
US20210333288A1 (en) * 2016-08-17 2021-10-28 Momenta Pharmaceuticals, Inc. Glycan oxonium ion profiling of glycosylated proteins
CN108072728A (zh) * 2016-11-16 2018-05-25 中国科学院大连化学物理研究所 一种基于数据依赖性质谱扫描模式的谱图库建立方法及其应用
CN108956789A (zh) * 2017-05-17 2018-12-07 中国科学院大连化学物理研究所 一种血清中免疫球蛋白g糖肽的绝对定量分析方法
CN110869768B (zh) * 2017-08-01 2023-11-21 安进公司 实时制备用于质谱分析的多肽样品的系统和方法
US10615016B2 (en) * 2017-09-07 2020-04-07 Thermo Fisher Scientific (Bremen) Gmbh Determining isotope ratios using mass spectrometry
CN108226516B (zh) * 2017-11-30 2020-05-19 中国农业科学院北京畜牧兽医研究所 一种相对定量分析肉鸡脂肪酸去饱和酶fads1的方法
CN108169397B (zh) * 2017-11-30 2019-11-15 中国农业科学院北京畜牧兽医研究所 一种相对定量分析肉鸡谷胱甘肽s转移酶gsta2的方法
CN109900815B (zh) * 2017-12-08 2021-06-29 中国科学院大连化学物理研究所 血清中IgG糖肽的绝对定量分析
BR112020011797A2 (pt) * 2018-01-31 2020-11-17 Regeneron Pharmaceuticals, Inc. métodos para identificar a glucuronilação de um medicamento com proteína, para aumentar a pureza de um medicamento com proteína e para identificação de medicamento com proteína pós-translacional modificado, e, medicamento com proteína
JP7205077B2 (ja) * 2018-05-10 2023-01-17 株式会社島津製作所 分析方法、処理装置、分析装置およびプログラム
CN110824096B (zh) * 2018-08-13 2022-11-29 齐鲁制药有限公司 一种蛋白错配二硫键的分析方法
CN110007019A (zh) * 2019-03-29 2019-07-12 绿城农科检测技术有限公司 一种同时定量检测母乳中三种抗体含量的方法
CN115427814A (zh) 2020-02-21 2022-12-02 根马布有限公司 用于定量糖蛋白的方法
CN111551739A (zh) * 2020-04-27 2020-08-18 杭州璞湃科技有限公司 一种免疫球蛋白a和免疫球蛋白a1的检测方法及其试剂盒
US20230228763A1 (en) * 2020-06-18 2023-07-20 Northwestern University Process for direct readout of immunoglobulins
JP7478040B2 (ja) * 2020-06-25 2024-05-02 シスメックス株式会社 Ａβペプチドの測定方法及びその方法に用いられる試薬組成物
CN111766323B (zh) * 2020-07-10 2022-06-14 中国检验检疫科学研究院 一种用于检测骆驼奶中掺入牛奶的特征肽组合及方法
CN113933409B (zh) * 2021-09-14 2023-11-14 上海中科新生命生物科技有限公司 基于液相色谱-质谱检测血清中特瑞普利单抗的方法
CN118221778A (zh) * 2024-04-25 2024-06-21 烟台大学 拟穴青蟹Scyreprocin蛋白的特征肽段及其在雌雄鉴别方面的应用

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AU2005263334C1 (en) * 2004-07-20 2011-01-20 Symphogen A/S A procedure for structural characterization of a recombinant polyclonal protein or a polyclonal cell line
BRPI0513706A (pt) 2004-07-20 2008-05-13 Symphogen As anticorpo policlonal recombinante anti-rhesus d e métodos de produção
JP4683572B2 (ja) * 2005-01-28 2011-05-18 塩野義製薬株式会社 組換え蛋白質の定量法
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Also Published As

Publication number Publication date
JP2013507603A (ja) 2013-03-04
IN2012DN03911A (es) 2015-09-04
KR20120100982A (ko) 2012-09-12
BR112012007815A2 (pt) 2018-03-20
AU2010305150A1 (en) 2012-04-05
CN102687020A (zh) 2012-09-19
US20120264155A1 (en) 2012-10-18
RU2012118620A (ru) 2013-11-20
ZA201202431B (en) 2012-12-27
CA2776508A1 (en) 2011-04-14
EP2486411A2 (en) 2012-08-15
WO2011042027A2 (en) 2011-04-14
IL218821A0 (en) 2012-06-28
WO2011042027A3 (en) 2011-09-22

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