IN2012DN03911A - - Google Patents

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Publication number
IN2012DN03911A
IN2012DN03911A IN3911DEN2012A IN2012DN03911A IN 2012DN03911 A IN2012DN03911 A IN 2012DN03911A IN 3911DEN2012 A IN3911DEN2012 A IN 3911DEN2012A IN 2012DN03911 A IN2012DN03911 A IN 2012DN03911A
Authority
IN
India
Prior art keywords
polyclonal antibodies
recombinant
recombinant polyclonal
quantitation
serum
Prior art date
Application number
Inventor
Torben Frandsen
Henrik Naested
Wagtbergsen Jette
Pernille Foged Jensen
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen As filed Critical Symphogen As
Publication of IN2012DN03911A publication Critical patent/IN2012DN03911A/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to an analytical method for quantitation of selected multiple recombinant proteins in a complex matrix such as recombinant polyclonal antibodies in serum or recombinant polyclonal antibodies expressed in a culture supernatant.
IN3911DEN2012 2009-10-09 2010-10-07 IN2012DN03911A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200901112 2009-10-09
US25623209P 2009-10-29 2009-10-29
PCT/DK2010/050258 WO2011042027A2 (en) 2009-10-09 2010-10-07 Multiplex quantitation of individual recombinant proteins in a mixture by signature peptides and mass spectrometry

Publications (1)

Publication Number Publication Date
IN2012DN03911A true IN2012DN03911A (en) 2015-09-04

Family

ID=42166454

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3911DEN2012 IN2012DN03911A (en) 2009-10-09 2010-10-07

Country Status (14)

Country Link
US (1) US20120264155A1 (en)
EP (1) EP2486411A2 (en)
JP (1) JP2013507603A (en)
KR (1) KR20120100982A (en)
CN (1) CN102687020A (en)
AU (1) AU2010305150A1 (en)
BR (1) BR112012007815A2 (en)
CA (1) CA2776508A1 (en)
IL (1) IL218821A0 (en)
IN (1) IN2012DN03911A (en)
MX (1) MX2012003538A (en)
RU (1) RU2012118620A (en)
WO (1) WO2011042027A2 (en)
ZA (1) ZA201202431B (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2241875A1 (en) * 2009-04-14 2010-10-20 Koninklijke Philips Electronics N.V. Up-concentration of organic microobjects for microscopic imaging.
ES2567276T3 (en) * 2011-05-12 2016-04-21 Genentech, Inc. LC-MS / MS method of monitoring multiple reactions to detect therapeutic antibodies in animal samples using frame-changing peptides
WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
WO2016022696A1 (en) 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
EP3268746A1 (en) 2015-03-12 2018-01-17 Mars, Incorporated Ultra high resolution mass spectrometry and methods of using the same
US10794916B2 (en) 2015-05-06 2020-10-06 Momenta Pharmaceuticals, Inc. Methods of glycoprotein analysis
CN105242050A (en) * 2015-09-16 2016-01-13 同济大学 Quantitative analysis method for intact protein under different physiological or pathological conditions
CN105218671A (en) * 2015-09-29 2016-01-06 李艳华 The preparation method of the full humanized antibody of a kind of infectious disease pathogens
GB201603291D0 (en) * 2016-02-25 2016-04-13 Binding Site Group The Ltd Antibodies
JP6943872B2 (en) * 2016-03-25 2021-10-06 ジェネンテック， インコーポレイテッド Multiple whole antibody and antibody complex drug quantification assay
WO2017205741A1 (en) 2016-05-27 2017-11-30 Genentech, Inc. Bioanalytical method for the characterization of site-specific antibody-drug conjugates
CN107525934A (en) * 2016-06-21 2017-12-29 张效龙 A kind of method for determining antibodies in blood amino acid sequence
US20210333288A1 (en) * 2016-08-17 2021-10-28 Momenta Pharmaceuticals, Inc. Glycan oxonium ion profiling of glycosylated proteins
CN108072728A (en) * 2016-11-16 2018-05-25 中国科学院大连化学物理研究所 A kind of spectrogram storehouse method for building up and its application based on data dependency scanning of the mass spectrum pattern
CN108956789A (en) * 2017-05-17 2018-12-07 中国科学院大连化学物理研究所 A kind of absolute quantification analysis method of Immunoglobulin in Serum G glycopeptide
BR112020001885A2 (en) * 2017-08-01 2020-08-04 Amgen Inc. systems and methods for real-time preparation of a polypeptide sample for analysis with mass spectrometry
US10615016B2 (en) * 2017-09-07 2020-04-07 Thermo Fisher Scientific (Bremen) Gmbh Determining isotope ratios using mass spectrometry
CN108169397B (en) * 2017-11-30 2019-11-15 中国农业科学院北京畜牧兽医研究所 A kind of method of relative quantitative assay broiler chicken glutathione s-transferase GSTA2
CN108226516B (en) * 2017-11-30 2020-05-19 中国农业科学院北京畜牧兽医研究所 Method for relatively quantitatively analyzing FADS1 of fatty acid desaturase of broiler chicken
CN109900815B (en) * 2017-12-08 2021-06-29 中国科学院大连化学物理研究所 Absolute quantitative analysis of IgG glycopeptides in serum
CA3084181A1 (en) * 2018-01-31 2019-08-08 Regeneron Pharmaceuticals, Inc. Glucuronylation as a new acidic post-translational modification on therapeutic monoclonal antibodies
JP7205077B2 (en) * 2018-05-10 2023-01-17 株式会社島津製作所 Analysis method, processor, analyzer and program
CN110824096B (en) * 2018-08-13 2022-11-29 齐鲁制药有限公司 Method for analyzing protein mismatching disulfide bond
CN110007019A (en) * 2019-03-29 2019-07-12 绿城农科检测技术有限公司 A kind of method that simultaneous quantitative detects three kinds of antibody contents in breast milk
US20230089196A1 (en) 2020-02-21 2023-03-23 Genmab B.V. Methods for the quantification of glycoproteins
CN111551739A (en) * 2020-04-27 2020-08-18 杭州璞湃科技有限公司 Detection method and kit for immunoglobulin A and immunoglobulin A1
US20230228763A1 (en) * 2020-06-18 2023-07-20 Northwestern University Process for direct readout of immunoglobulins
JP7478040B2 (en) * 2020-06-25 2024-05-02 シスメックス株式会社 METHOD FOR MEASURING Aβ PEPTIDE AND REAGENT COMPOSITION USED IN THE METHOD
CN111766323B (en) * 2020-07-10 2022-06-14 中国检验检疫科学研究院 Characteristic peptide combination and method for detecting milk doped in camel milk
CN113933409B (en) * 2021-09-14 2023-11-14 上海中科新生命生物科技有限公司 Method for detecting terlipressin Li Shan antibody in serum based on liquid chromatography-mass spectrometry

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040033530A1 (en) * 2002-04-08 2004-02-19 Awrey Donald E. High throughput purification, characterization and identification of recombinant proteins
AU2003287017B2 (en) 2002-10-03 2011-10-06 Norman Leigh Anderson High sensitivity quantitation of peptides by mass spectrometry
EP1766388A4 (en) * 2004-06-09 2008-08-20 Anderson Forschung Group Llc Stable isotope labeled polypeptide standards for protein quantitation
ES2332524T3 (en) * 2004-07-20 2010-02-08 Symphogen A/S A PROCEDURE FOR THE CHARACTERIZATION OF A POLYCLONAL CELL LINE.
EP1771483A1 (en) 2004-07-20 2007-04-11 Symphogen A/S Anti-rhesus d recombinant polyclonal antibody and methods of manufacture
US20090298196A1 (en) * 2005-01-28 2009-12-03 Uwabe Ken-Ichiro Quantitative measurement method for recombinant protein
JP2006300758A (en) * 2005-04-21 2006-11-02 Apro Life Science Institute Inc Method for quantitatively determining target protein contained in living body origin sample by using internal standard peptide
AU2006322445B2 (en) 2005-12-05 2011-05-12 Symphogen A/S Anti-orthopoxvirus recombinant polyclonal antibody
EP2004686A1 (en) 2006-03-06 2008-12-24 Symphogen A/S Recombinant polyclonal antibody for treatment of respiratory syncytial virus infections
KR101676622B1 (en) 2007-03-01 2016-11-17 심포젠 에이/에스 Recombinant anti-epidermal growth factor receptor antibody compositions
CA2678628A1 (en) 2007-03-06 2008-09-12 Symphogen A/S Recombinant antibodies for treatment of respiratory syncytial virus infections
US20090000419A1 (en) * 2007-06-29 2009-01-01 Harley-Davidson Motor Company Group, Inc. Handlebar assembly with axial locking feature

Also Published As

Publication number Publication date
RU2012118620A (en) 2013-11-20
WO2011042027A2 (en) 2011-04-14
MX2012003538A (en) 2012-08-03
ZA201202431B (en) 2012-12-27
EP2486411A2 (en) 2012-08-15
CA2776508A1 (en) 2011-04-14
IL218821A0 (en) 2012-06-28
AU2010305150A1 (en) 2012-04-05
CN102687020A (en) 2012-09-19
JP2013507603A (en) 2013-03-04
BR112012007815A2 (en) 2018-03-20
WO2011042027A3 (en) 2011-09-22
KR20120100982A (en) 2012-09-12
US20120264155A1 (en) 2012-10-18

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