JP2012513386A - 核酸の精製方法 - Google Patents

核酸の精製方法 Download PDF

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Publication number
JP2012513386A
JP2012513386A JP2011541520A JP2011541520A JP2012513386A JP 2012513386 A JP2012513386 A JP 2012513386A JP 2011541520 A JP2011541520 A JP 2011541520A JP 2011541520 A JP2011541520 A JP 2011541520A JP 2012513386 A JP2012513386 A JP 2012513386A
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nucleic acid
support
binding
propyl
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クリストフ エルバッハー，
ローラント ファビス，
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キアゲン ゲーエムベーハー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
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  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
JP2011541520A 2008-12-23 2009-12-23 核酸の精製方法 Withdrawn JP2012513386A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102008063001.2 2008-12-23
DE102008063001A DE102008063001A1 (de) 2008-12-23 2008-12-23 Nukleinsäureaufreinigungsverfahren
PCT/EP2009/067909 WO2010072834A1 (de) 2008-12-23 2009-12-23 Nukleinsäureaufreinigungsverfahren

Related Child Applications (1)

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JP2016012103A Division JP6072945B2 (ja) 2008-12-23 2016-01-26 核酸の精製方法

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JP2012513386A true JP2012513386A (ja) 2012-06-14

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JP2011541520A Withdrawn JP2012513386A (ja) 2008-12-23 2009-12-23 核酸の精製方法
JP2016012103A Active JP6072945B2 (ja) 2008-12-23 2016-01-26 核酸の精製方法

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JP2016012103A Active JP6072945B2 (ja) 2008-12-23 2016-01-26 核酸の精製方法

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US (1) US9102935B2 (https=)
EP (1) EP2382315B1 (https=)
JP (2) JP2012513386A (https=)
CN (1) CN102264902B (https=)
AU (1) AU2009332900B2 (https=)
DE (1) DE102008063001A1 (https=)
WO (1) WO2010072834A1 (https=)

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WO2019131760A1 (ja) * 2017-12-27 2019-07-04 東レ株式会社 核酸の回収方法

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DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
JP5899731B2 (ja) 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
EP2760999A1 (en) 2011-09-26 2014-08-06 Qiagen GmbH Methods for separating nucleic acids by size
EP2761001B1 (en) * 2011-09-26 2018-08-01 Qiagen GmbH Rapid method for isolating extracellular nucleic acids
EP2771462A4 (en) * 2011-10-27 2015-04-08 Ge Healthcare Bio Sciences Ab PURIFICATION OF NUCLEIC ACIDS
EP2854982B1 (en) * 2012-05-31 2017-11-01 Agency For Science, Technology And Research Chromatographic purification of polynucleotides with negatively charged particles
CN103789297A (zh) * 2012-10-26 2014-05-14 上海医脉赛科技有限公司 快速纯化核酸的方法及试剂盒
WO2014090838A1 (en) 2012-12-11 2014-06-19 Qiagen Gmbh Preparation of silica particles
WO2014144767A1 (en) * 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
WO2014152027A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Manufacturing methods for production of rna transcripts
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
CN106604992B (zh) * 2014-09-10 2021-06-18 康特姆斯集团有限公司 用于分离生物大分子的吸附剂材料
WO2016065218A1 (en) 2014-10-23 2016-04-28 Corning Incorporated Polymer-encapsulated magnetic nanoparticles
KR102488291B1 (ko) * 2015-03-20 2023-01-13 도레이 카부시키가이샤 핵산의 회수 방법
JP6787916B2 (ja) 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
EP3430135B1 (en) 2016-03-19 2026-02-11 QIAGEN GmbH Stabilization of rna
WO2017223176A1 (en) 2016-06-24 2017-12-28 Modernatx, Inc. Methods and apparatus for filtration
CN108102881A (zh) * 2018-02-05 2018-06-01 中国科学院苏州纳米技术与纳米仿生研究所 具有微结构阵列的微流控芯片及核酸固相纯化提取方法
CN108456678A (zh) * 2018-07-06 2018-08-28 大连元和健路医学检验实验室有限公司 一种快速提取基因组dna的方法
CN108795929A (zh) * 2018-07-06 2018-11-13 大连元和健路医学检验实验室有限公司 一种dna分子筛柱子的制备方法
US12377409B2 (en) 2019-03-29 2025-08-05 Sumitomo Chemical Company, Limited Inorganic porous carrier, and production method for nucleic acid using same
WO2021122846A1 (en) 2019-12-16 2021-06-24 Qiagen Gmbh Enrichment method
EP4355873A1 (en) 2021-06-17 2024-04-24 QIAGEN GmbH Method for isolating non-vesicular mirna
WO2023084369A1 (en) 2021-11-12 2023-05-19 3M Innovative Properties Company Method of processing polynucleic acids
CN115537416A (zh) * 2022-09-29 2022-12-30 杭州联川生物技术股份有限公司 一种纯化寡核苷酸的方法和试剂盒

Citations (7)

* Cited by examiner, † Cited by third party
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JPS646755A (en) * 1987-01-21 1989-01-11 Du Pont Chromatography support body for oligonucleotide separation
JPH09157282A (ja) * 1995-12-04 1997-06-17 Japan Synthetic Rubber Co Ltd 核酸吸着剤
JPH10502052A (ja) * 1994-04-08 1998-02-24 ハイブライドン インコーポレイテッド 陰イオン交換クロマトグラフィーを用いるオリゴデオキシヌクレオチド・ホスホロチエオエートの精製
JPH10506987A (ja) * 1994-09-23 1998-07-07 マッシー ユニヴァーシティー クロマトグラフ用樹脂およびその使用方法
JP2002543979A (ja) * 1999-05-14 2002-12-24 プロメガ コーポレイション ｐＨ依存型イオン交換マトリックス及び核酸の単離におけるその使用法
JP2003104996A (ja) * 2001-09-28 2003-04-09 Hitachi Maxell Ltd 核酸結合用磁性担体およびその製造方法
JP2004521881A (ja) * 2000-12-14 2004-07-22 ディーエヌエー リサーチ イノヴェイションズ リミテッド 核酸の分離

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US5079600A (en) * 1987-03-06 1992-01-07 Schnur Joel M High resolution patterning on solid substrates
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US6103479A (en) * 1996-05-30 2000-08-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
WO1999029703A2 (en) 1997-12-06 1999-06-17 Dna Research Instruments Limited Isolation of nucleic acids
KR100714988B1 (ko) 2005-08-09 2007-05-09 삼성전자주식회사 산화막이 표면에 형성된 실리콘 구조물 표면을 이용한ｄｎａ 정제 방법 및 정제장치
WO2008097342A2 (en) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions and methods for isolation of biological molecules
WO2008035991A2 (en) * 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS646755A (en) * 1987-01-21 1989-01-11 Du Pont Chromatography support body for oligonucleotide separation
JPH10502052A (ja) * 1994-04-08 1998-02-24 ハイブライドン インコーポレイテッド 陰イオン交換クロマトグラフィーを用いるオリゴデオキシヌクレオチド・ホスホロチエオエートの精製
JPH10506987A (ja) * 1994-09-23 1998-07-07 マッシー ユニヴァーシティー クロマトグラフ用樹脂およびその使用方法
JPH09157282A (ja) * 1995-12-04 1997-06-17 Japan Synthetic Rubber Co Ltd 核酸吸着剤
JP2002543979A (ja) * 1999-05-14 2002-12-24 プロメガ コーポレイション ｐＨ依存型イオン交換マトリックス及び核酸の単離におけるその使用法
JP2004521881A (ja) * 2000-12-14 2004-07-22 ディーエヌエー リサーチ イノヴェイションズ リミテッド 核酸の分離
JP2003104996A (ja) * 2001-09-28 2003-04-09 Hitachi Maxell Ltd 核酸結合用磁性担体およびその製造方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019131760A1 (ja) * 2017-12-27 2019-07-04 東レ株式会社 核酸の回収方法
US11685915B2 (en) 2017-12-27 2023-06-27 Toray Industries, Inc. Method of collecting nucleic acid

Also Published As

Publication number Publication date
JP2016135784A (ja) 2016-07-28
WO2010072834A1 (de) 2010-07-01
EP2382315B1 (de) 2015-09-23
AU2009332900B2 (en) 2014-05-29
EP2382315A1 (de) 2011-11-02
US9102935B2 (en) 2015-08-11
CN102264902A (zh) 2011-11-30
DE102008063001A1 (de) 2010-06-24
CN102264902B (zh) 2018-06-01
AU2009332900A1 (en) 2010-07-01
JP6072945B2 (ja) 2017-02-01
US20110319506A1 (en) 2011-12-29

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