DE102008063001A1 - Nukleinsäureaufreinigungsverfahren - Google Patents
Nukleinsäureaufreinigungsverfahren Download PDFInfo
- Publication number
- DE102008063001A1 DE102008063001A1 DE102008063001A DE102008063001A DE102008063001A1 DE 102008063001 A1 DE102008063001 A1 DE 102008063001A1 DE 102008063001 A DE102008063001 A DE 102008063001A DE 102008063001 A DE102008063001 A DE 102008063001A DE 102008063001 A1 DE102008063001 A1 DE 102008063001A1
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- Germany
- Prior art keywords
- groups
- binding
- group
- nucleic acid
- dimethylamino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 title claims abstract description 54
- 238000001821 nucleic acid purification Methods 0.000 title description 2
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 167
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 167
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 167
- 238000010828 elution Methods 0.000 claims abstract description 60
- 150000003839 salts Chemical class 0.000 claims abstract description 28
- 238000000746 purification Methods 0.000 claims abstract description 27
- 230000008569 process Effects 0.000 claims abstract description 16
- 230000006735 deficit Effects 0.000 claims abstract description 7
- 239000012876 carrier material Substances 0.000 claims description 71
- 229910052757 nitrogen Inorganic materials 0.000 claims description 67
- -1 and its derivatives Polymers 0.000 claims description 48
- 239000000463 material Substances 0.000 claims description 48
- 239000003446 ligand Substances 0.000 claims description 46
- 239000003999 initiator Substances 0.000 claims description 42
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims description 33
- 150000001875 compounds Chemical class 0.000 claims description 30
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 27
- 150000004756 silanes Chemical class 0.000 claims description 25
- 239000000203 mixture Substances 0.000 claims description 24
- 239000000178 monomer Substances 0.000 claims description 23
- 229920000642 polymer Polymers 0.000 claims description 22
- 229910000077 silane Inorganic materials 0.000 claims description 21
- 229920002818 (Hydroxyethyl)methacrylate Polymers 0.000 claims description 15
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 claims description 15
- 239000012149 elution buffer Substances 0.000 claims description 14
- 238000007306 functionalization reaction Methods 0.000 claims description 12
- 230000004048 modification Effects 0.000 claims description 11
- 238000012986 modification Methods 0.000 claims description 11
- 238000002444 silanisation Methods 0.000 claims description 11
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 claims description 9
- 230000007935 neutral effect Effects 0.000 claims description 9
- 125000000217 alkyl group Chemical group 0.000 claims description 8
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 8
- 150000004820 halides Chemical class 0.000 claims description 8
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 claims description 8
- 239000000377 silicon dioxide Substances 0.000 claims description 8
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 7
- GXGGOCHUJJTJGF-UHFFFAOYSA-N 2-(chloromethyl)prop-2-enyl-trimethoxysilane Chemical group CO[Si](OC)(OC)CC(=C)CCl GXGGOCHUJJTJGF-UHFFFAOYSA-N 0.000 claims description 6
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- BAPJBEWLBFYGME-UHFFFAOYSA-N Methyl acrylate Chemical compound COC(=O)C=C BAPJBEWLBFYGME-UHFFFAOYSA-N 0.000 claims description 6
- FQPSGWSUVKBHSU-UHFFFAOYSA-N methacrylamide Chemical compound CC(=C)C(N)=O FQPSGWSUVKBHSU-UHFFFAOYSA-N 0.000 claims description 6
- VILCJCGEZXAXTO-UHFFFAOYSA-N 2,2,2-tetramine Chemical compound NCCNCCNCCN VILCJCGEZXAXTO-UHFFFAOYSA-N 0.000 claims description 5
- 125000000022 2-aminoethyl group Chemical group [H]C([*])([H])C([H])([H])N([H])[H] 0.000 claims description 5
- RPNUMPOLZDHAAY-UHFFFAOYSA-N Diethylenetriamine Chemical compound NCCNCCN RPNUMPOLZDHAAY-UHFFFAOYSA-N 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- 239000012148 binding buffer Substances 0.000 claims description 5
- 239000001273 butane Substances 0.000 claims description 5
- 229920001577 copolymer Polymers 0.000 claims description 5
- 150000002009 diols Chemical group 0.000 claims description 5
- VOZRXNHHFUQHIL-UHFFFAOYSA-N glycidyl methacrylate Chemical compound CC(=C)C(=O)OCC1CO1 VOZRXNHHFUQHIL-UHFFFAOYSA-N 0.000 claims description 5
- 239000001257 hydrogen Substances 0.000 claims description 5
- 229910052739 hydrogen Inorganic materials 0.000 claims description 5
- BQZUWMDQFGDJQM-UHFFFAOYSA-N n',n',2-trimethylhex-2-enehydrazide Chemical compound CCCC=C(C)C(=O)NN(C)C BQZUWMDQFGDJQM-UHFFFAOYSA-N 0.000 claims description 5
- 229920000058 polyacrylate Polymers 0.000 claims description 5
- GAWIXWVDTYZWAW-UHFFFAOYSA-N C[CH]O Chemical group C[CH]O GAWIXWVDTYZWAW-UHFFFAOYSA-N 0.000 claims description 4
- 239000005046 Chlorosilane Substances 0.000 claims description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 4
- 229910004298 SiO 2 Inorganic materials 0.000 claims description 4
- GTTSNKDQDACYLV-UHFFFAOYSA-N Trihydroxybutane Chemical compound CCCC(O)(O)O GTTSNKDQDACYLV-UHFFFAOYSA-N 0.000 claims description 4
- 150000001720 carbohydrates Chemical class 0.000 claims description 4
- KOPOQZFJUQMUML-UHFFFAOYSA-N chlorosilane Chemical class Cl[SiH3] KOPOQZFJUQMUML-UHFFFAOYSA-N 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 235000011187 glycerol Nutrition 0.000 claims description 4
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 claims description 4
- 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 claims description 4
- PNJWIWWMYCMZRO-UHFFFAOYSA-N pent‐4‐en‐2‐one Natural products CC(=O)CC=C PNJWIWWMYCMZRO-UHFFFAOYSA-N 0.000 claims description 4
- 229920000570 polyether Chemical group 0.000 claims description 4
- 150000003077 polyols Chemical group 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 4
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims description 4
- ADXGNEYLLLSOAR-UHFFFAOYSA-N tasosartan Chemical compound C12=NC(C)=NC(C)=C2CCC(=O)N1CC(C=C1)=CC=C1C1=CC=CC=C1C=1N=NNN=1 ADXGNEYLLLSOAR-UHFFFAOYSA-N 0.000 claims description 4
- FAGUFWYHJQFNRV-UHFFFAOYSA-N tetraethylenepentamine Chemical compound NCCNCCNCCNCCN FAGUFWYHJQFNRV-UHFFFAOYSA-N 0.000 claims description 4
- YXMISKNUHHOXFT-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) prop-2-enoate Chemical compound C=CC(=O)ON1C(=O)CCC1=O YXMISKNUHHOXFT-UHFFFAOYSA-N 0.000 claims description 3
- 125000004169 (C1-C6) alkyl group Chemical group 0.000 claims description 3
- QRIMLDXJAPZHJE-UHFFFAOYSA-N 2,3-dihydroxypropyl 2-methylprop-2-enoate Chemical compound CC(=C)C(=O)OCC(O)CO QRIMLDXJAPZHJE-UHFFFAOYSA-N 0.000 claims description 3
- QHVBLSNVXDSMEB-UHFFFAOYSA-N 2-(diethylamino)ethyl prop-2-enoate Chemical compound CCN(CC)CCOC(=O)C=C QHVBLSNVXDSMEB-UHFFFAOYSA-N 0.000 claims description 3
- JKNCOURZONDCGV-UHFFFAOYSA-N 2-(dimethylamino)ethyl 2-methylprop-2-enoate Chemical compound CN(C)CCOC(=O)C(C)=C JKNCOURZONDCGV-UHFFFAOYSA-N 0.000 claims description 3
- SVYHMICYJHWXIN-UHFFFAOYSA-N 2-[di(propan-2-yl)amino]ethyl 2-methylprop-2-enoate Chemical compound CC(C)N(C(C)C)CCOC(=O)C(C)=C SVYHMICYJHWXIN-UHFFFAOYSA-N 0.000 claims description 3
- SCBAFGFZXWUDBS-UHFFFAOYSA-N 2-methyl-N',N'-di(propan-2-yl)pent-2-enehydrazide Chemical compound C(C)C=C(C(=O)NN(C(C)C)C(C)C)C SCBAFGFZXWUDBS-UHFFFAOYSA-N 0.000 claims description 3
- JTEROQLCSIVTLH-UHFFFAOYSA-N 2-methylidene-N',N'-di(propan-2-yl)butanehydrazide Chemical compound C(C)C(C(=O)NN(C(C)C)C(C)C)=C JTEROQLCSIVTLH-UHFFFAOYSA-N 0.000 claims description 3
- UQJWFFCXCTXHRV-UHFFFAOYSA-N 2-methylidene-N',N'-di(propan-2-yl)pentanehydrazide Chemical compound C(CC)C(C(=O)NN(C(C)C)C(C)C)=C UQJWFFCXCTXHRV-UHFFFAOYSA-N 0.000 claims description 3
- UBKOBOIZDIWIFU-UHFFFAOYSA-N 3-(oxiran-2-ylmethoxy)propyl 2-methylprop-2-enoate Chemical compound CC(=C)C(=O)OCCCOCC1CO1 UBKOBOIZDIWIFU-UHFFFAOYSA-N 0.000 claims description 3
- 229910018072 Al 2 O 3 Inorganic materials 0.000 claims description 3
- KCIPGHYGHSNNLE-UHFFFAOYSA-N C(C)C=C(C(=O)NN(C)C)C Chemical compound C(C)C=C(C(=O)NN(C)C)C KCIPGHYGHSNNLE-UHFFFAOYSA-N 0.000 claims description 3
- 229920001661 Chitosan Polymers 0.000 claims description 3
- JIGUQPWFLRLWPJ-UHFFFAOYSA-N Ethyl acrylate Chemical compound CCOC(=O)C=C JIGUQPWFLRLWPJ-UHFFFAOYSA-N 0.000 claims description 3
- XPUCSPJMOKVKSE-UHFFFAOYSA-N N',N'-diethyl-2-methylhex-2-enehydrazide Chemical compound C(CC)C=C(C(=O)NN(CC)CC)C XPUCSPJMOKVKSE-UHFFFAOYSA-N 0.000 claims description 3
- 239000004721 Polyphenylene oxide Chemical group 0.000 claims description 3
- 150000001336 alkenes Chemical group 0.000 claims description 3
- CQEYYJKEWSMYFG-UHFFFAOYSA-N butyl acrylate Chemical compound CCCCOC(=O)C=C CQEYYJKEWSMYFG-UHFFFAOYSA-N 0.000 claims description 3
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- JZYGBKYWEPIHGU-UHFFFAOYSA-N n',n'-diethyl-2-methylidenepentanehydrazide Chemical compound CCCC(=C)C(=O)NN(CC)CC JZYGBKYWEPIHGU-UHFFFAOYSA-N 0.000 claims description 3
- QTECWEOCNYGONE-UHFFFAOYSA-N n',n'-dimethyl-2-methylidenebutanehydrazide Chemical compound CCC(=C)C(=O)NN(C)C QTECWEOCNYGONE-UHFFFAOYSA-N 0.000 claims description 3
- YHOSNAAUPKDRMI-UHFFFAOYSA-N n,n-di(propan-2-yl)prop-2-enamide Chemical compound CC(C)N(C(C)C)C(=O)C=C YHOSNAAUPKDRMI-UHFFFAOYSA-N 0.000 claims description 3
- OVHHHVAVHBHXAK-UHFFFAOYSA-N n,n-diethylprop-2-enamide Chemical compound CCN(CC)C(=O)C=C OVHHHVAVHBHXAK-UHFFFAOYSA-N 0.000 claims description 3
- FCTYAARUVAKIOB-UHFFFAOYSA-N n,n-dimethylprop-2-enamide;2-methylprop-2-enamide Chemical compound CC(=C)C(N)=O.CN(C)C(=O)C=C FCTYAARUVAKIOB-UHFFFAOYSA-N 0.000 claims description 3
- GUAQVFRUPZBRJQ-UHFFFAOYSA-N n-(3-aminopropyl)-2-methylprop-2-enamide Chemical compound CC(=C)C(=O)NCCCN GUAQVFRUPZBRJQ-UHFFFAOYSA-N 0.000 claims description 3
- QNCPCGJTMRRTOH-UHFFFAOYSA-N n-[1-(dimethylamino)propyl]prop-2-enamide Chemical compound CCC(N(C)C)NC(=O)C=C QNCPCGJTMRRTOH-UHFFFAOYSA-N 0.000 claims description 3
- SYSQUGFVNFXIIT-UHFFFAOYSA-N n-[4-(1,3-benzoxazol-2-yl)phenyl]-4-nitrobenzenesulfonamide Chemical class C1=CC([N+](=O)[O-])=CC=C1S(=O)(=O)NC1=CC=C(C=2OC3=CC=CC=C3N=2)C=C1 SYSQUGFVNFXIIT-UHFFFAOYSA-N 0.000 claims description 3
- PNXMTCDJUBJHQJ-UHFFFAOYSA-N propyl prop-2-enoate Chemical compound CCCOC(=O)C=C PNXMTCDJUBJHQJ-UHFFFAOYSA-N 0.000 claims description 3
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 claims description 3
- FZHAPNGMFPVSLP-UHFFFAOYSA-N silanamine Chemical class [SiH3]N FZHAPNGMFPVSLP-UHFFFAOYSA-N 0.000 claims description 3
- 125000001302 tertiary amino group Chemical group 0.000 claims description 3
- ZMANZCXQSJIPKH-UHFFFAOYSA-N triethylamine Natural products CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 3
- 150000004072 triols Chemical group 0.000 claims description 3
- ZFFMLCVRJBZUDZ-UHFFFAOYSA-N 2,3-dimethylbutane Chemical group CC(C)C(C)C ZFFMLCVRJBZUDZ-UHFFFAOYSA-N 0.000 claims description 2
- UNVFWCQQWZUPLB-UHFFFAOYSA-N 3-[dimethoxy(pentan-3-yloxy)silyl]propan-1-amine Chemical compound CCC(CC)O[Si](OC)(OC)CCCN UNVFWCQQWZUPLB-UHFFFAOYSA-N 0.000 claims description 2
- 229920000936 Agarose Polymers 0.000 claims description 2
- 229920002307 Dextran Polymers 0.000 claims description 2
- 239000004677 Nylon Substances 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 239000004793 Polystyrene Substances 0.000 claims description 2
- 239000012506 Sephacryl® Substances 0.000 claims description 2
- 229920005654 Sephadex Polymers 0.000 claims description 2
- 239000012507 Sephadex™ Substances 0.000 claims description 2
- 229910010413 TiO 2 Inorganic materials 0.000 claims description 2
- GEIAQOFPUVMAGM-UHFFFAOYSA-N ZrO Inorganic materials [Zr]=O GEIAQOFPUVMAGM-UHFFFAOYSA-N 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 239000000017 hydrogel Substances 0.000 claims description 2
- MCJIGEUBDWRFSC-UHFFFAOYSA-N n',n'-diethyl-2-methylidenebutanehydrazide Chemical compound CCN(CC)NC(=O)C(=C)CC MCJIGEUBDWRFSC-UHFFFAOYSA-N 0.000 claims description 2
- FYBNBLQCLXTBCV-UHFFFAOYSA-N n,n-di(propan-2-yl)-3-trimethoxysilylpropan-1-amine Chemical compound CO[Si](OC)(OC)CCCN(C(C)C)C(C)C FYBNBLQCLXTBCV-UHFFFAOYSA-N 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- 229920000620 organic polymer Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- 229920002635 polyurethane Polymers 0.000 claims description 2
- 239000004814 polyurethane Substances 0.000 claims description 2
- 125000002947 alkylene group Chemical group 0.000 claims 2
- 150000002118 epoxides Chemical group 0.000 claims 2
- 125000001033 ether group Chemical group 0.000 claims 2
- 125000001475 halogen functional group Chemical group 0.000 claims 2
- 125000005462 imide group Chemical group 0.000 claims 2
- JQQGOMZGMWOLIJ-UHFFFAOYSA-N n,n',n'-triethyl-2-methylprop-2-enehydrazide Chemical compound CCN(CC)N(CC)C(=O)C(C)=C JQQGOMZGMWOLIJ-UHFFFAOYSA-N 0.000 claims 2
- VRQCYSFKHSYREX-UHFFFAOYSA-N 1-[dimethoxy(propyl)silyl]oxy-n,n-dimethylmethanamine Chemical compound CCC[Si](OC)(OC)OCN(C)C VRQCYSFKHSYREX-UHFFFAOYSA-N 0.000 claims 1
- OWPUOLBODXJOKH-UHFFFAOYSA-N 2,3-dihydroxypropyl prop-2-enoate Chemical compound OCC(O)COC(=O)C=C OWPUOLBODXJOKH-UHFFFAOYSA-N 0.000 claims 1
- OMIGHNLMNHATMP-UHFFFAOYSA-N 2-hydroxyethyl prop-2-enoate Chemical compound OCCOC(=O)C=C OMIGHNLMNHATMP-UHFFFAOYSA-N 0.000 claims 1
- GEDOVYSTEQZNIS-UHFFFAOYSA-N 3-(oxiran-2-ylmethoxy)propyl prop-2-enoate Chemical compound C=CC(=O)OCCCOCC1CO1 GEDOVYSTEQZNIS-UHFFFAOYSA-N 0.000 claims 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 claims 1
- 239000004698 Polyethylene Substances 0.000 claims 1
- SUPCQIBBMFXVTL-UHFFFAOYSA-N ethyl 2-methylprop-2-enoate Chemical compound CCOC(=O)C(C)=C SUPCQIBBMFXVTL-UHFFFAOYSA-N 0.000 claims 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims 1
- GAYDVWRHTHHMGO-UHFFFAOYSA-N n,n',n'-triethylprop-2-enehydrazide Chemical compound CCN(CC)N(CC)C(=O)C=C GAYDVWRHTHHMGO-UHFFFAOYSA-N 0.000 claims 1
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 claims 1
- 125000001280 n-hexyl group Chemical group C(CCCCC)* 0.000 claims 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 claims 1
- RPQRDASANLAFCM-UHFFFAOYSA-N oxiran-2-ylmethyl prop-2-enoate Chemical compound C=CC(=O)OCC1CO1 RPQRDASANLAFCM-UHFFFAOYSA-N 0.000 claims 1
- 229920000573 polyethylene Polymers 0.000 claims 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 claims 1
- 239000012071 phase Substances 0.000 description 40
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 24
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Sampling And Sample Adjustment (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Priority Applications (8)
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| DE102008063001A DE102008063001A1 (de) | 2008-12-23 | 2008-12-23 | Nukleinsäureaufreinigungsverfahren |
| CN200980152958.XA CN102264902B (zh) | 2008-12-23 | 2009-12-23 | 核酸纯化方法 |
| EP09804132.0A EP2382315B1 (de) | 2008-12-23 | 2009-12-23 | Nukleinsäureaufreinigungsverfahren |
| JP2011541520A JP2012513386A (ja) | 2008-12-23 | 2009-12-23 | 核酸の精製方法 |
| US13/141,878 US9102935B2 (en) | 2008-12-23 | 2009-12-23 | Nucleic acid purification method |
| PCT/EP2009/067909 WO2010072834A1 (de) | 2008-12-23 | 2009-12-23 | Nukleinsäureaufreinigungsverfahren |
| AU2009332900A AU2009332900B2 (en) | 2008-12-23 | 2009-12-23 | Nucleic acid purification method |
| JP2016012103A JP6072945B2 (ja) | 2008-12-23 | 2016-01-26 | 核酸の精製方法 |
Applications Claiming Priority (1)
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| DE102008063001A DE102008063001A1 (de) | 2008-12-23 | 2008-12-23 | Nukleinsäureaufreinigungsverfahren |
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| DE102008063001A1 true DE102008063001A1 (de) | 2010-06-24 |
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| DE102008063001A Withdrawn DE102008063001A1 (de) | 2008-12-23 | 2008-12-23 | Nukleinsäureaufreinigungsverfahren |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9102935B2 (https=) |
| EP (1) | EP2382315B1 (https=) |
| JP (2) | JP2012513386A (https=) |
| CN (1) | CN102264902B (https=) |
| AU (1) | AU2009332900B2 (https=) |
| DE (1) | DE102008063001A1 (https=) |
| WO (1) | WO2010072834A1 (https=) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013045432A1 (en) | 2011-09-26 | 2013-04-04 | Qiagen Gmbh | Rapid method for isolating extracellular nucleic acids |
| WO2016198571A1 (en) | 2015-06-10 | 2016-12-15 | Qiagen Gmbh | Method for isolating extracellular nucleic acids using anion exchange particles |
| WO2017162518A1 (en) | 2016-03-19 | 2017-09-28 | Qiagen Gmbh | Stabilization of rna |
| US9938520B2 (en) | 2012-12-11 | 2018-04-10 | Qiagen Gmbh | Preparation of silica particles |
| EP3272866A4 (en) * | 2015-03-20 | 2018-10-24 | Toray Industries, Inc. | Method for collecting nucleic acid |
| WO2021122846A1 (en) | 2019-12-16 | 2021-06-24 | Qiagen Gmbh | Enrichment method |
| WO2022263500A1 (en) | 2021-06-17 | 2022-12-22 | Qiagen Gmbh | Method for isolating non-vesicular mirna |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102008063003A1 (de) * | 2008-12-23 | 2010-06-24 | Qiagen Gmbh | Nukleinsäureaufreinigungsverfahren |
| JP5899731B2 (ja) | 2011-09-13 | 2016-04-06 | ソニー株式会社 | 核酸精製方法、核酸抽出方法、及び核酸精製用キット |
| US20140243216A1 (en) | 2011-09-26 | 2014-08-28 | Qiagen Gmbh | Methods for separating nucleic acids by size |
| US9340828B2 (en) * | 2011-10-27 | 2016-05-17 | Ge Healthcare Bio-Sciences Ab | Purification of nucleic acid |
| KR20150023331A (ko) * | 2012-05-31 | 2015-03-05 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | 음으로 대전된 입자들로의 폴리뉴클레오티드들의 크로마토그래피 정제 |
| CN103789297A (zh) * | 2012-10-26 | 2014-05-14 | 上海医脉赛科技有限公司 | 快速纯化核酸的方法及试剂盒 |
| EP2971165A4 (en) | 2013-03-15 | 2016-11-23 | Moderna Therapeutics Inc | DISSOLUTION OF DNA FRAGMENTS IN MRNA MANUFACTURING METHODS |
| WO2014152027A1 (en) | 2013-03-15 | 2014-09-25 | Moderna Therapeutics, Inc. | Manufacturing methods for production of rna transcripts |
| US10590161B2 (en) | 2013-03-15 | 2020-03-17 | Modernatx, Inc. | Ion exchange purification of mRNA |
| SG11201701783QA (en) * | 2014-09-10 | 2017-04-27 | Quantumdx Group Ltd | Sorbent material for separating bio-macromolecules |
| JP2017539080A (ja) | 2014-10-23 | 2017-12-28 | コーニング インコーポレイテッド | 高分子被包磁性ナノ粒子 |
| WO2017223176A1 (en) | 2016-06-24 | 2017-12-28 | Modernatx, Inc. | Methods and apparatus for filtration |
| JP7238766B2 (ja) | 2017-12-27 | 2023-03-14 | 東レ株式会社 | 核酸の回収方法 |
| CN108102881A (zh) * | 2018-02-05 | 2018-06-01 | 中国科学院苏州纳米技术与纳米仿生研究所 | 具有微结构阵列的微流控芯片及核酸固相纯化提取方法 |
| CN108456678A (zh) * | 2018-07-06 | 2018-08-28 | 大连元和健路医学检验实验室有限公司 | 一种快速提取基因组dna的方法 |
| CN108795929A (zh) * | 2018-07-06 | 2018-11-13 | 大连元和健路医学检验实验室有限公司 | 一种dna分子筛柱子的制备方法 |
| JP7368455B2 (ja) | 2019-03-29 | 2023-10-24 | 住友化学株式会社 | 無機多孔質担体、及びこれを用いた核酸の製造方法 |
| WO2023084369A1 (en) | 2021-11-12 | 2023-05-19 | 3M Innovative Properties Company | Method of processing polynucleic acids |
| CN115537416A (zh) * | 2022-09-29 | 2022-12-30 | 杭州联川生物技术股份有限公司 | 一种纯化寡核苷酸的方法和试剂盒 |
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- 2009-12-23 CN CN200980152958.XA patent/CN102264902B/zh active Active
- 2009-12-23 EP EP09804132.0A patent/EP2382315B1/de active Active
- 2009-12-23 JP JP2011541520A patent/JP2012513386A/ja not_active Withdrawn
- 2009-12-23 AU AU2009332900A patent/AU2009332900B2/en active Active
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| WO1999029703A2 (en) | 1997-12-06 | 1999-06-17 | Dna Research Instruments Limited | Isolation of nucleic acids |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP3412773A1 (en) * | 2011-09-26 | 2018-12-12 | QIAGEN GmbH | Rapid method for isolating extracellular nucleic acids |
| EP4159857A1 (en) * | 2011-09-26 | 2023-04-05 | QIAGEN GmbH | Rapid method for isolating extracellular nucleic acids |
| US11021736B2 (en) | 2011-09-26 | 2021-06-01 | Qiagen Gmbh | Rapid method for isolating extracellular nucleic acids |
| WO2013045432A1 (en) | 2011-09-26 | 2013-04-04 | Qiagen Gmbh | Rapid method for isolating extracellular nucleic acids |
| US10184145B2 (en) | 2011-09-26 | 2019-01-22 | Qiagen Gmbh | Rapid method for isolating extracellular nucleic acids |
| US9938520B2 (en) | 2012-12-11 | 2018-04-10 | Qiagen Gmbh | Preparation of silica particles |
| EP3272866A4 (en) * | 2015-03-20 | 2018-10-24 | Toray Industries, Inc. | Method for collecting nucleic acid |
| US11104896B2 (en) | 2015-06-10 | 2021-08-31 | Qiagen Gmbh | Method for isolating extracellular nucleic acids using anion exchange particles |
| WO2016198571A1 (en) | 2015-06-10 | 2016-12-15 | Qiagen Gmbh | Method for isolating extracellular nucleic acids using anion exchange particles |
| US12031125B2 (en) | 2015-06-10 | 2024-07-09 | Qiagen Gmbh | Method for isolating extracellular nucleic acids using anion exchange particles |
| WO2017162518A1 (en) | 2016-03-19 | 2017-09-28 | Qiagen Gmbh | Stabilization of rna |
| WO2021122846A1 (en) | 2019-12-16 | 2021-06-24 | Qiagen Gmbh | Enrichment method |
| WO2022263500A1 (en) | 2021-06-17 | 2022-12-22 | Qiagen Gmbh | Method for isolating non-vesicular mirna |
Also Published As
| Publication number | Publication date |
|---|---|
| US9102935B2 (en) | 2015-08-11 |
| JP2012513386A (ja) | 2012-06-14 |
| JP6072945B2 (ja) | 2017-02-01 |
| AU2009332900A1 (en) | 2010-07-01 |
| CN102264902B (zh) | 2018-06-01 |
| CN102264902A (zh) | 2011-11-30 |
| EP2382315A1 (de) | 2011-11-02 |
| EP2382315B1 (de) | 2015-09-23 |
| JP2016135784A (ja) | 2016-07-28 |
| WO2010072834A1 (de) | 2010-07-01 |
| US20110319506A1 (en) | 2011-12-29 |
| AU2009332900B2 (en) | 2014-05-29 |
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