JP2012510500A5 - - Google Patents
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- Publication number
- JP2012510500A5 JP2012510500A5 JP2011538995A JP2011538995A JP2012510500A5 JP 2012510500 A5 JP2012510500 A5 JP 2012510500A5 JP 2011538995 A JP2011538995 A JP 2011538995A JP 2011538995 A JP2011538995 A JP 2011538995A JP 2012510500 A5 JP2012510500 A5 JP 2012510500A5
- Authority
- JP
- Japan
- Prior art keywords
- factor
- gla
- polypeptide
- elution
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 17
- 229920001184 polypeptide Polymers 0.000 claims 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims 10
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims 8
- 102100022641 Coagulation factor IX Human genes 0.000 claims 6
- 102100023804 Coagulation factor VII Human genes 0.000 claims 6
- 108010076282 Factor IX Proteins 0.000 claims 6
- 108010023321 Factor VII Proteins 0.000 claims 6
- 108010054265 Factor VIIa Proteins 0.000 claims 6
- 238000010828 elution Methods 0.000 claims 6
- 229960004222 factor ix Drugs 0.000 claims 6
- 229940012413 factor vii Drugs 0.000 claims 6
- 229940012414 factor viia Drugs 0.000 claims 6
- UHBYWPGGCSDKFX-VKHMYHEASA-N gamma-carboxy-L-glutamic acid Chemical compound OC(=O)[C@@H](N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-VKHMYHEASA-N 0.000 claims 5
- 239000000463 material Substances 0.000 claims 5
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims 4
- 239000005695 Ammonium acetate Substances 0.000 claims 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 4
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims 4
- 229940043376 ammonium acetate Drugs 0.000 claims 4
- 235000019257 ammonium acetate Nutrition 0.000 claims 4
- 235000019270 ammonium chloride Nutrition 0.000 claims 4
- 238000005349 anion exchange Methods 0.000 claims 4
- 239000001632 sodium acetate Substances 0.000 claims 4
- 235000017281 sodium acetate Nutrition 0.000 claims 4
- 239000012149 elution buffer Substances 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 2
- 150000003839 salts Chemical group 0.000 claims 2
- 108010014173 Factor X Proteins 0.000 claims 1
- 102100031487 Growth arrest-specific protein 6 Human genes 0.000 claims 1
- 101000923005 Homo sapiens Growth arrest-specific protein 6 Proteins 0.000 claims 1
- 101100216047 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) gla-1 gene Proteins 0.000 claims 1
- 108090000573 Osteocalcin Proteins 0.000 claims 1
- 102000004067 Osteocalcin Human genes 0.000 claims 1
- 101800004937 Protein C Proteins 0.000 claims 1
- 102000017975 Protein C Human genes 0.000 claims 1
- 108010066124 Protein S Proteins 0.000 claims 1
- 229940096437 Protein S Drugs 0.000 claims 1
- 108010094028 Prothrombin Proteins 0.000 claims 1
- 102100027378 Prothrombin Human genes 0.000 claims 1
- 101800001700 Saposin-D Proteins 0.000 claims 1
- 102100028885 Vitamin K-dependent protein S Human genes 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 239000006167 equilibration buffer Substances 0.000 claims 1
- 229940012426 factor x Drugs 0.000 claims 1
- 238000004255 ion exchange chromatography Methods 0.000 claims 1
- 108010057546 matrix Gla protein Proteins 0.000 claims 1
- 102000043253 matrix Gla protein Human genes 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 108010025221 plasma protein Z Proteins 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 229960000856 protein c Drugs 0.000 claims 1
- 229940039716 prothrombin Drugs 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08170454.6 | 2008-12-02 | ||
| EP08170454 | 2008-12-02 | ||
| PCT/EP2009/066149 WO2010063717A1 (en) | 2008-12-02 | 2009-12-01 | Polypeptide purification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012510500A JP2012510500A (ja) | 2012-05-10 |
| JP2012510500A5 true JP2012510500A5 (https=) | 2013-01-10 |
| JP5788803B2 JP5788803B2 (ja) | 2015-10-07 |
Family
ID=40404199
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011538995A Active JP5788803B2 (ja) | 2008-12-02 | 2009-12-01 | ポリペプチド精製 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20110287518A1 (https=) |
| EP (1) | EP2373677B1 (https=) |
| JP (1) | JP5788803B2 (https=) |
| KR (1) | KR101821143B1 (https=) |
| CN (1) | CN102239175A (https=) |
| AU (1) | AU2009324204B2 (https=) |
| BR (1) | BRPI0922716A2 (https=) |
| CA (1) | CA2742246A1 (https=) |
| ES (1) | ES2576109T3 (https=) |
| MX (1) | MX2011005474A (https=) |
| RU (1) | RU2544860C2 (https=) |
| WO (1) | WO2010063717A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011073235A1 (en) | 2009-12-18 | 2011-06-23 | Csl Ltd. | Method of purifying polypeptides |
| BR112012017696A2 (pt) | 2010-01-18 | 2017-10-03 | Novo Nordisk Healthcare Ag | Purificação de fatores de coagulação de sangue |
| CN107915769A (zh) * | 2011-03-29 | 2018-04-17 | 葛兰素史密斯克莱有限责任公司 | 用于蛋白纯化的缓冲液体系 |
| JP6116565B2 (ja) * | 2011-08-25 | 2017-04-19 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 陽イオンおよび陰イオン交換クロマトグラフィー法 |
| US10066224B2 (en) | 2015-02-25 | 2018-09-04 | Omrix Biopharmaceuticals Ltd. | Method for purifying and quantifying thrombin and its degradation polypeptides |
| WO2016207328A1 (en) * | 2015-06-24 | 2016-12-29 | Glycotope Gmbh | PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES |
| KR102470282B1 (ko) * | 2016-07-15 | 2022-11-24 | 에프. 호프만-라 로슈 아게 | Ｐｅｇ화 에리트로포이에틴을 정제하기 위한 방법 |
| EP4045917A1 (en) * | 2019-10-14 | 2022-08-24 | Pierce Biotechnology, Inc. | Peptide purification formulations and methods |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981952A (en) | 1988-10-04 | 1991-01-01 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
| IT1262899B (it) | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| CN101818137A (zh) * | 2003-03-18 | 2010-09-01 | 诺和诺德医疗保健公司 | 生产含有gla残基的丝氨酸蛋白酶的方法 |
| EP2360171A1 (en) * | 2004-12-23 | 2011-08-24 | Novo Nordisk Health Care AG | Reduction of the content of protein contaminants in compositions comprising a vitamin K-dependent protein of interest |
| DK2125866T3 (da) * | 2007-02-28 | 2013-07-29 | Baxter Int | Fremgangsmåde til rensning af rekombinant blodkoagulationsfaktor IX, som er beriget med sulfaterede og/eller phosphorylerede molekyler |
-
2009
- 2009-12-01 KR KR1020117014183A patent/KR101821143B1/ko not_active Expired - Fee Related
- 2009-12-01 BR BRPI0922716A patent/BRPI0922716A2/pt not_active Application Discontinuation
- 2009-12-01 CA CA2742246A patent/CA2742246A1/en not_active Abandoned
- 2009-12-01 AU AU2009324204A patent/AU2009324204B2/en not_active Ceased
- 2009-12-01 WO PCT/EP2009/066149 patent/WO2010063717A1/en not_active Ceased
- 2009-12-01 CN CN2009801490787A patent/CN102239175A/zh active Pending
- 2009-12-01 JP JP2011538995A patent/JP5788803B2/ja active Active
- 2009-12-01 US US13/131,757 patent/US20110287518A1/en not_active Abandoned
- 2009-12-01 RU RU2011125656/04A patent/RU2544860C2/ru active
- 2009-12-01 ES ES09793497.0T patent/ES2576109T3/es active Active
- 2009-12-01 EP EP09793497.0A patent/EP2373677B1/en active Active
- 2009-12-01 MX MX2011005474A patent/MX2011005474A/es active IP Right Grant
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