JP2012510500A5 - - Google Patents
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- Publication number
- JP2012510500A5 JP2012510500A5 JP2011538995A JP2011538995A JP2012510500A5 JP 2012510500 A5 JP2012510500 A5 JP 2012510500A5 JP 2011538995 A JP2011538995 A JP 2011538995A JP 2011538995 A JP2011538995 A JP 2011538995A JP 2012510500 A5 JP2012510500 A5 JP 2012510500A5
- Authority
- JP
- Japan
- Prior art keywords
- factor
- gla
- polypeptide
- elution
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 17
- 229920001184 polypeptide Polymers 0.000 claims 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims 10
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims 8
- 102100022641 Coagulation factor IX Human genes 0.000 claims 6
- 102100023804 Coagulation factor VII Human genes 0.000 claims 6
- 108010076282 Factor IX Proteins 0.000 claims 6
- 108010023321 Factor VII Proteins 0.000 claims 6
- 108010054265 Factor VIIa Proteins 0.000 claims 6
- 238000010828 elution Methods 0.000 claims 6
- 229960004222 factor ix Drugs 0.000 claims 6
- 229940012413 factor vii Drugs 0.000 claims 6
- 229940012414 factor viia Drugs 0.000 claims 6
- UHBYWPGGCSDKFX-VKHMYHEASA-N gamma-carboxy-L-glutamic acid Chemical compound OC(=O)[C@@H](N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-VKHMYHEASA-N 0.000 claims 5
- 239000000463 material Substances 0.000 claims 5
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims 4
- 239000005695 Ammonium acetate Substances 0.000 claims 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 4
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims 4
- 229940043376 ammonium acetate Drugs 0.000 claims 4
- 235000019257 ammonium acetate Nutrition 0.000 claims 4
- 235000019270 ammonium chloride Nutrition 0.000 claims 4
- 238000005349 anion exchange Methods 0.000 claims 4
- 239000001632 sodium acetate Substances 0.000 claims 4
- 235000017281 sodium acetate Nutrition 0.000 claims 4
- 239000012149 elution buffer Substances 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 2
- 150000003839 salts Chemical group 0.000 claims 2
- 108010014173 Factor X Proteins 0.000 claims 1
- 102100031487 Growth arrest-specific protein 6 Human genes 0.000 claims 1
- 101000923005 Homo sapiens Growth arrest-specific protein 6 Proteins 0.000 claims 1
- 101100216047 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) gla-1 gene Proteins 0.000 claims 1
- 108090000573 Osteocalcin Proteins 0.000 claims 1
- 102000004067 Osteocalcin Human genes 0.000 claims 1
- 101800004937 Protein C Proteins 0.000 claims 1
- 102000017975 Protein C Human genes 0.000 claims 1
- 108010066124 Protein S Proteins 0.000 claims 1
- 229940096437 Protein S Drugs 0.000 claims 1
- 108010094028 Prothrombin Proteins 0.000 claims 1
- 102100027378 Prothrombin Human genes 0.000 claims 1
- 101800001700 Saposin-D Proteins 0.000 claims 1
- 102100028885 Vitamin K-dependent protein S Human genes 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 239000006167 equilibration buffer Substances 0.000 claims 1
- 229940012426 factor x Drugs 0.000 claims 1
- 238000004255 ion exchange chromatography Methods 0.000 claims 1
- 108010057546 matrix Gla protein Proteins 0.000 claims 1
- 102000043253 matrix Gla protein Human genes 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 108010025221 plasma protein Z Proteins 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 229960000856 protein c Drugs 0.000 claims 1
- 229940039716 prothrombin Drugs 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08170454.6 | 2008-12-02 | ||
| EP08170454 | 2008-12-02 | ||
| PCT/EP2009/066149 WO2010063717A1 (en) | 2008-12-02 | 2009-12-01 | Polypeptide purification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012510500A JP2012510500A (ja) | 2012-05-10 |
| JP2012510500A5 true JP2012510500A5 (https=) | 2013-01-10 |
| JP5788803B2 JP5788803B2 (ja) | 2015-10-07 |
Family
ID=40404199
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011538995A Active JP5788803B2 (ja) | 2008-12-02 | 2009-12-01 | ポリペプチド精製 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20110287518A1 (https=) |
| EP (1) | EP2373677B1 (https=) |
| JP (1) | JP5788803B2 (https=) |
| KR (1) | KR101821143B1 (https=) |
| CN (1) | CN102239175A (https=) |
| AU (1) | AU2009324204B2 (https=) |
| BR (1) | BRPI0922716A2 (https=) |
| CA (1) | CA2742246A1 (https=) |
| ES (1) | ES2576109T3 (https=) |
| MX (1) | MX2011005474A (https=) |
| RU (1) | RU2544860C2 (https=) |
| WO (1) | WO2010063717A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104193798A (zh) * | 2009-12-18 | 2014-12-10 | Csl有限公司 | 纯化多肽的方法 |
| EP2526115B1 (en) | 2010-01-18 | 2018-08-01 | Novo Nordisk Health Care AG | Purification of blood coagulation factors |
| KR102058254B1 (ko) * | 2011-03-29 | 2019-12-20 | 글락소스미스클라인 엘엘씨 | 단백질 정제용 완충제 시스템 |
| CN103703015B (zh) * | 2011-08-25 | 2019-07-05 | 霍夫曼-拉罗奇有限公司 | 阳离子和阴离子交换层析法 |
| CN107406840B (zh) | 2015-02-25 | 2021-05-28 | 奥姆里克斯生物药品有限公司 | 用于纯化和定量凝血酶及其降解多肽的方法 |
| WO2016207328A1 (en) * | 2015-06-24 | 2016-12-29 | Glycotope Gmbh | PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES |
| HRP20202077T1 (hr) * | 2016-07-15 | 2021-02-19 | F. Hoffmann - La Roche Ag | Postupak za pročišćavanje pegiliranog eritropoetina |
| KR20220079844A (ko) * | 2019-10-14 | 2022-06-14 | 피어스 바이오테크놀로지, 인크 | 펩티드 정제 제제 및 방법 |
| JP2023090132A (ja) * | 2021-12-17 | 2023-06-29 | Ｋｍバイオロジクス株式会社 | ビタミンＫ依存性タンパク質のγ－カルボキシグルタミン酸修飾度の分析方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981952A (en) * | 1988-10-04 | 1991-01-01 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
| IT1262899B (it) | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| RU2364626C2 (ru) * | 2003-03-18 | 2009-08-20 | Ново Нордиск Хелт Кэр Аг | Способ получения, способ очистки и способ стабилизации полипептидов фактора vii, ix и x |
| ES2395544T3 (es) * | 2004-12-23 | 2013-02-13 | Novo Nordisk Health Care Ag | Reducción del contenido de contaminantes proteínicos en composiciones que comprenden una proteína dependiente de la vitamina K de interés |
| WO2008104372A1 (en) * | 2007-02-28 | 2008-09-04 | Baxter International Inc. | Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules |
-
2009
- 2009-12-01 AU AU2009324204A patent/AU2009324204B2/en not_active Ceased
- 2009-12-01 JP JP2011538995A patent/JP5788803B2/ja active Active
- 2009-12-01 RU RU2011125656/04A patent/RU2544860C2/ru active
- 2009-12-01 BR BRPI0922716A patent/BRPI0922716A2/pt not_active Application Discontinuation
- 2009-12-01 CA CA2742246A patent/CA2742246A1/en not_active Abandoned
- 2009-12-01 EP EP09793497.0A patent/EP2373677B1/en active Active
- 2009-12-01 CN CN2009801490787A patent/CN102239175A/zh active Pending
- 2009-12-01 ES ES09793497.0T patent/ES2576109T3/es active Active
- 2009-12-01 WO PCT/EP2009/066149 patent/WO2010063717A1/en not_active Ceased
- 2009-12-01 US US13/131,757 patent/US20110287518A1/en not_active Abandoned
- 2009-12-01 KR KR1020117014183A patent/KR101821143B1/ko not_active Expired - Fee Related
- 2009-12-01 MX MX2011005474A patent/MX2011005474A/es active IP Right Grant
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