AU2009324204B2 - Polypeptide purification - Google Patents
Polypeptide purification Download PDFInfo
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- AU2009324204B2 AU2009324204B2 AU2009324204A AU2009324204A AU2009324204B2 AU 2009324204 B2 AU2009324204 B2 AU 2009324204B2 AU 2009324204 A AU2009324204 A AU 2009324204A AU 2009324204 A AU2009324204 A AU 2009324204A AU 2009324204 B2 AU2009324204 B2 AU 2009324204B2
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- polypeptide
- gla
- factor
- sample
- buffer
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- 210000001672 ovary Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N phenylalanine group Chemical group N[C@@H](CC1=CC=CC=C1)C(=O)O COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000003805 procoagulant Substances 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 229940070376 protein Drugs 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
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- 238000012163 sequencing technique Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 108020001568 subdomains Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- 230000032258 transport Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 108010087967 type I signal peptidase Proteins 0.000 description 1
- 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6437—Coagulation factor VIIa (3.4.21.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08170454.6 | 2008-12-02 | ||
| EP08170454 | 2008-12-02 | ||
| PCT/EP2009/066149 WO2010063717A1 (en) | 2008-12-02 | 2009-12-01 | Polypeptide purification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2009324204A1 AU2009324204A1 (en) | 2010-06-10 |
| AU2009324204B2 true AU2009324204B2 (en) | 2014-10-16 |
Family
ID=40404199
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2009324204A Ceased AU2009324204B2 (en) | 2008-12-02 | 2009-12-01 | Polypeptide purification |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20110287518A1 (https=) |
| EP (1) | EP2373677B1 (https=) |
| JP (1) | JP5788803B2 (https=) |
| KR (1) | KR101821143B1 (https=) |
| CN (1) | CN102239175A (https=) |
| AU (1) | AU2009324204B2 (https=) |
| BR (1) | BRPI0922716A2 (https=) |
| CA (1) | CA2742246A1 (https=) |
| ES (1) | ES2576109T3 (https=) |
| MX (1) | MX2011005474A (https=) |
| RU (1) | RU2544860C2 (https=) |
| WO (1) | WO2010063717A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104193798A (zh) * | 2009-12-18 | 2014-12-10 | Csl有限公司 | 纯化多肽的方法 |
| EP2526115B1 (en) | 2010-01-18 | 2018-08-01 | Novo Nordisk Health Care AG | Purification of blood coagulation factors |
| KR102058254B1 (ko) * | 2011-03-29 | 2019-12-20 | 글락소스미스클라인 엘엘씨 | 단백질 정제용 완충제 시스템 |
| CN103703015B (zh) * | 2011-08-25 | 2019-07-05 | 霍夫曼-拉罗奇有限公司 | 阳离子和阴离子交换层析法 |
| CN107406840B (zh) | 2015-02-25 | 2021-05-28 | 奥姆里克斯生物药品有限公司 | 用于纯化和定量凝血酶及其降解多肽的方法 |
| WO2016207328A1 (en) * | 2015-06-24 | 2016-12-29 | Glycotope Gmbh | PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES |
| HRP20202077T1 (hr) * | 2016-07-15 | 2021-02-19 | F. Hoffmann - La Roche Ag | Postupak za pročišćavanje pegiliranog eritropoetina |
| KR20220079844A (ko) * | 2019-10-14 | 2022-06-14 | 피어스 바이오테크놀로지, 인크 | 펩티드 정제 제제 및 방법 |
| JP2023090132A (ja) * | 2021-12-17 | 2023-06-29 | Ｋｍバイオロジクス株式会社 | ビタミンＫ依存性タンパク質のγ－カルボキシグルタミン酸修飾度の分析方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0363126A2 (en) * | 1988-10-04 | 1990-04-11 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1262899B (it) | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| RU2364626C2 (ru) * | 2003-03-18 | 2009-08-20 | Ново Нордиск Хелт Кэр Аг | Способ получения, способ очистки и способ стабилизации полипептидов фактора vii, ix и x |
| ES2395544T3 (es) * | 2004-12-23 | 2013-02-13 | Novo Nordisk Health Care Ag | Reducción del contenido de contaminantes proteínicos en composiciones que comprenden una proteína dependiente de la vitamina K de interés |
| WO2008104372A1 (en) * | 2007-02-28 | 2008-09-04 | Baxter International Inc. | Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules |
-
2009
- 2009-12-01 AU AU2009324204A patent/AU2009324204B2/en not_active Ceased
- 2009-12-01 JP JP2011538995A patent/JP5788803B2/ja active Active
- 2009-12-01 RU RU2011125656/04A patent/RU2544860C2/ru active
- 2009-12-01 BR BRPI0922716A patent/BRPI0922716A2/pt not_active Application Discontinuation
- 2009-12-01 CA CA2742246A patent/CA2742246A1/en not_active Abandoned
- 2009-12-01 EP EP09793497.0A patent/EP2373677B1/en active Active
- 2009-12-01 CN CN2009801490787A patent/CN102239175A/zh active Pending
- 2009-12-01 ES ES09793497.0T patent/ES2576109T3/es active Active
- 2009-12-01 WO PCT/EP2009/066149 patent/WO2010063717A1/en not_active Ceased
- 2009-12-01 US US13/131,757 patent/US20110287518A1/en not_active Abandoned
- 2009-12-01 KR KR1020117014183A patent/KR101821143B1/ko not_active Expired - Fee Related
- 2009-12-01 MX MX2011005474A patent/MX2011005474A/es active IP Right Grant
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0363126A2 (en) * | 1988-10-04 | 1990-04-11 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
Non-Patent Citations (1)
| Title |
|---|
| YAN SC BET AL; NATURE vol. 8, no. 7, (1 July 1990), pages 655-661 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2373677B1 (en) | 2016-03-23 |
| EP2373677A1 (en) | 2011-10-12 |
| AU2009324204A1 (en) | 2010-06-10 |
| CN102239175A (zh) | 2011-11-09 |
| BRPI0922716A2 (pt) | 2016-11-01 |
| US20110287518A1 (en) | 2011-11-24 |
| MX2011005474A (es) | 2011-06-16 |
| RU2544860C2 (ru) | 2015-03-20 |
| ES2576109T3 (es) | 2016-07-05 |
| JP2012510500A (ja) | 2012-05-10 |
| JP5788803B2 (ja) | 2015-10-07 |
| KR101821143B1 (ko) | 2018-01-23 |
| CA2742246A1 (en) | 2010-06-10 |
| RU2011125656A (ru) | 2013-01-10 |
| WO2010063717A1 (en) | 2010-06-10 |
| KR20110099251A (ko) | 2011-09-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |