ES2576109T3 - Purificación de polipéptidos - Google Patents

Purificación de polipéptidos Download PDF

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Publication number
ES2576109T3
ES2576109T3 ES09793497.0T ES09793497T ES2576109T3 ES 2576109 T3 ES2576109 T3 ES 2576109T3 ES 09793497 T ES09793497 T ES 09793497T ES 2576109 T3 ES2576109 T3 ES 2576109T3
Authority
ES
Spain
Prior art keywords
polypeptide
gla
buffer
sample
anion exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09793497.0T
Other languages
English (en)
Spanish (es)
Inventor
Jais Rose Bjelke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk Health Care AG
Original Assignee
Novo Nordisk Health Care AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Health Care AG filed Critical Novo Nordisk Health Care AG
Application granted granted Critical
Publication of ES2576109T3 publication Critical patent/ES2576109T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
ES09793497.0T 2008-12-02 2009-12-01 Purificación de polipéptidos Active ES2576109T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08170454 2008-12-02
EP08170454 2008-12-02
PCT/EP2009/066149 WO2010063717A1 (en) 2008-12-02 2009-12-01 Polypeptide purification

Publications (1)

Publication Number Publication Date
ES2576109T3 true ES2576109T3 (es) 2016-07-05

Family

ID=40404199

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09793497.0T Active ES2576109T3 (es) 2008-12-02 2009-12-01 Purificación de polipéptidos

Country Status (12)

Country Link
US (1) US20110287518A1 (https=)
EP (1) EP2373677B1 (https=)
JP (1) JP5788803B2 (https=)
KR (1) KR101821143B1 (https=)
CN (1) CN102239175A (https=)
AU (1) AU2009324204B2 (https=)
BR (1) BRPI0922716A2 (https=)
CA (1) CA2742246A1 (https=)
ES (1) ES2576109T3 (https=)
MX (1) MX2011005474A (https=)
RU (1) RU2544860C2 (https=)
WO (1) WO2010063717A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104193798A (zh) * 2009-12-18 2014-12-10 Csl有限公司 纯化多肽的方法
EP2526115B1 (en) 2010-01-18 2018-08-01 Novo Nordisk Health Care AG Purification of blood coagulation factors
KR102058254B1 (ko) * 2011-03-29 2019-12-20 글락소스미스클라인 엘엘씨 단백질 정제용 완충제 시스템
CN103703015B (zh) * 2011-08-25 2019-07-05 霍夫曼-拉罗奇有限公司 阳离子和阴离子交换层析法
CN107406840B (zh) 2015-02-25 2021-05-28 奥姆里克斯生物药品有限公司 用于纯化和定量凝血酶及其降解多肽的方法
WO2016207328A1 (en) * 2015-06-24 2016-12-29 Glycotope Gmbh PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES
HRP20202077T1 (hr) * 2016-07-15 2021-02-19 F. Hoffmann - La Roche Ag Postupak za pročišćavanje pegiliranog eritropoetina
KR20220079844A (ko) * 2019-10-14 2022-06-14 피어스 바이오테크놀로지, 인크 펩티드 정제 제제 및 방법
JP2023090132A (ja) * 2021-12-17 2023-06-29 Ｋｍバイオロジクス株式会社 ビタミンＫ依存性タンパク質のγ－カルボキシグルタミン酸修飾度の分析方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
IT1262899B (it) 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
RU2364626C2 (ru) * 2003-03-18 2009-08-20 Ново Нордиск Хелт Кэр Аг Способ получения, способ очистки и способ стабилизации полипептидов фактора vii, ix и x
ES2395544T3 (es) * 2004-12-23 2013-02-13 Novo Nordisk Health Care Ag Reducción del contenido de contaminantes proteínicos en composiciones que comprenden una proteína dependiente de la vitamina K de interés
WO2008104372A1 (en) * 2007-02-28 2008-09-04 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules

Also Published As

Publication number Publication date
EP2373677B1 (en) 2016-03-23
EP2373677A1 (en) 2011-10-12
AU2009324204A1 (en) 2010-06-10
CN102239175A (zh) 2011-11-09
BRPI0922716A2 (pt) 2016-11-01
US20110287518A1 (en) 2011-11-24
MX2011005474A (es) 2011-06-16
RU2544860C2 (ru) 2015-03-20
JP2012510500A (ja) 2012-05-10
JP5788803B2 (ja) 2015-10-07
KR101821143B1 (ko) 2018-01-23
CA2742246A1 (en) 2010-06-10
RU2011125656A (ru) 2013-01-10
WO2010063717A1 (en) 2010-06-10
KR20110099251A (ko) 2011-09-07
AU2009324204B2 (en) 2014-10-16

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