JP2007532477A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2007532477A5 JP2007532477A5 JP2006538022A JP2006538022A JP2007532477A5 JP 2007532477 A5 JP2007532477 A5 JP 2007532477A5 JP 2006538022 A JP2006538022 A JP 2006538022A JP 2006538022 A JP2006538022 A JP 2006538022A JP 2007532477 A5 JP2007532477 A5 JP 2007532477A5
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- protein
- substrate
- ion exchange
- hydroxyapatite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 26
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 26
- 239000000758 substrate Substances 0.000 claims description 26
- 102000004169 proteins and genes Human genes 0.000 claims description 24
- 108090000623 proteins and genes Proteins 0.000 claims description 24
- 238000002360 preparation method Methods 0.000 claims description 22
- 239000011347 resin Substances 0.000 claims description 22
- 229920005989 resin Polymers 0.000 claims description 22
- 238000005342 ion exchange Methods 0.000 claims description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 239000011534 wash buffer Substances 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 12
- 238000004587 chromatography analysis Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 239000012149 elution buffer Substances 0.000 claims description 7
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 239000012160 loading buffer Substances 0.000 claims description 4
- 238000004255 ion exchange chromatography Methods 0.000 claims description 2
- 239000001488 sodium phosphate Substances 0.000 claims description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 2
- 238000001042 affinity chromatography Methods 0.000 claims 1
- 239000000178 monomer Substances 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000005349 anion exchange Methods 0.000 description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 239000000872 buffer Substances 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000919 ceramic Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229910052586 apatite Inorganic materials 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000006167 equilibration buffer Substances 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51401803P | 2003-10-27 | 2003-10-27 | |
| US60/514,018 | 2003-10-27 | ||
| US52333503P | 2003-11-20 | 2003-11-20 | |
| US60/523,335 | 2003-11-20 | ||
| PCT/US2004/032883 WO2005044856A2 (en) | 2003-10-27 | 2004-10-06 | Removal of high molecular weight aggregates using hydroxyapatite chromatography |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012013648A Division JP5490156B2 (ja) | 2003-10-27 | 2012-01-26 | ヒドロキシアパタイトクロマトグラフィーを用いる高分子量凝集体の取り出し |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2007532477A JP2007532477A (ja) | 2007-11-15 |
| JP2007532477A5 true JP2007532477A5 (https=) | 2012-03-22 |
| JP5004586B2 JP5004586B2 (ja) | 2012-08-22 |
Family
ID=34576743
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006538022A Expired - Lifetime JP5004586B2 (ja) | 2003-10-27 | 2004-10-06 | ヒドロキシアパタイトクロマトグラフィーを用いる高分子量凝集体の取り出し |
| JP2012013648A Expired - Lifetime JP5490156B2 (ja) | 2003-10-27 | 2012-01-26 | ヒドロキシアパタイトクロマトグラフィーを用いる高分子量凝集体の取り出し |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012013648A Expired - Lifetime JP5490156B2 (ja) | 2003-10-27 | 2012-01-26 | ヒドロキシアパタイトクロマトグラフィーを用いる高分子量凝集体の取り出し |
Country Status (19)
| Country | Link |
|---|---|
| US (2) | US9469672B2 (https=) |
| EP (2) | EP2336172B1 (https=) |
| JP (2) | JP5004586B2 (https=) |
| KR (2) | KR101221862B1 (https=) |
| CN (2) | CN102276717B (https=) |
| AU (1) | AU2004286938B2 (https=) |
| BR (2) | BRPI0415887B1 (https=) |
| CA (1) | CA2543193C (https=) |
| CO (1) | CO5690649A2 (https=) |
| DK (2) | DK2336172T3 (https=) |
| EC (1) | ECSP066583A (https=) |
| ES (2) | ES2418830T3 (https=) |
| IL (1) | IL175229A (https=) |
| MX (1) | MXPA06004472A (https=) |
| NZ (1) | NZ547315A (https=) |
| PL (2) | PL1678208T3 (https=) |
| PT (2) | PT1678208E (https=) |
| RU (2) | RU2409591C2 (https=) |
| WO (1) | WO2005044856A2 (https=) |
Families Citing this family (71)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
| KR101221862B1 (ko) * | 2003-10-27 | 2013-01-14 | 와이어쓰 엘엘씨 | 하이드록시아파타이트 크로마토그래피를 사용한 고분자량응집체의 제거 |
| EP1869065B1 (en) * | 2005-03-11 | 2020-05-06 | Wyeth LLC | A method of weak partitioning chromatography |
| ZA200710330B (en) * | 2005-06-17 | 2009-12-30 | Wyeth Corp | Methods of purifying FC region containing proteins |
| KR20080025174A (ko) * | 2005-06-23 | 2008-03-19 | 메디뮨 인코포레이티드 | 응집 및 단편화 프로파일이 최적화된 항체 제제 |
| AR058140A1 (es) * | 2005-10-24 | 2008-01-23 | Wyeth Corp | Metodo de produccion proteica utilizando compuestos anti-senescencia |
| BRPI0620316A2 (pt) * | 2005-12-21 | 2011-11-08 | Wyeth Corp | formulações de proteìnas com viscosidades reduzida e seus usos |
| JPWO2007123242A1 (ja) | 2006-04-25 | 2009-09-10 | 東ソー株式会社 | ＩｇＧ精製用分離剤、及びそれを用いたＩｇＧ単量体の精製方法 |
| JP2010510963A (ja) * | 2006-06-14 | 2010-04-08 | グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー | セラミックヒドロキシアパタイトを使用する抗体の精製方法 |
| WO2008025747A1 (en) * | 2006-08-28 | 2008-03-06 | Ares Trading S.A. | Process for the purification of fc-fusion proteins |
| SI2061803T2 (sl) | 2006-08-28 | 2023-01-31 | Ares Trading S.A. | Postopek za čiščenje proteinov, ki vsebujejo FC |
| CN101511387A (zh) * | 2006-09-08 | 2009-08-19 | 惠氏公司 | 使用亲和色谱法纯化蛋白质的精氨酸洗涤液 |
| AU2007294731B2 (en) | 2006-09-13 | 2014-04-17 | Abbvie Inc. | Cell culture improvements |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| US7999085B2 (en) * | 2007-01-09 | 2011-08-16 | Bio-Rad Laboratories, Inc. | Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers |
| US7691980B2 (en) | 2007-01-09 | 2010-04-06 | Bio-Rad Laboratories, Inc. | Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers |
| ES2366593T3 (es) * | 2007-01-26 | 2011-10-21 | Merck Serono S.A. | Purificación de proteínas de fusión taci-fc empleando la tecnología de cuerpos grasos. |
| TW200902708A (en) | 2007-04-23 | 2009-01-16 | Wyeth Corp | Methods of protein production using anti-senescence compounds |
| WO2009054226A1 (ja) * | 2007-10-26 | 2009-04-30 | Asahi Kasei Chemicals Corporation | たんぱく質の精製方法 |
| WO2009126603A1 (en) | 2008-04-08 | 2009-10-15 | Bio-Rad Laboratories, Inc. | Chromatography purification of antibodies |
| BRPI0823046B1 (pt) | 2008-08-14 | 2021-12-14 | Genentech, Inc | Método para purificar um anticorpo monoclonal de uma composição compreendendo o anticorpo monoclonal e pelo menos uma proteína de ovário de hamster chinês (chop) |
| NZ592095A (en) | 2008-10-20 | 2013-01-25 | Abbott Lab | Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography |
| BRPI0920572A8 (pt) | 2008-10-20 | 2015-10-27 | Abbott Lab | Inativação viral durante a purificação dos anticorpos |
| CA2738243C (en) | 2008-10-29 | 2020-09-29 | Wyeth Llc | Formulations of single domain antigen binding molecules |
| BRPI0919879A2 (pt) | 2008-10-29 | 2016-02-16 | Wyeth Llc | métodos para purificação de moléculas de ligação a antígeno de domínio único |
| US8058407B2 (en) | 2008-10-31 | 2011-11-15 | Wyeth Llc | Purification of acidic proteins using ceramic hydroxyapatite chromatography |
| WO2010147686A2 (en) * | 2009-03-11 | 2010-12-23 | Wyeth Llc | Methods of purifying small modular immunopharmaceutical proteins |
| EP2424875B1 (en) | 2009-04-29 | 2016-01-06 | Bio-Rad Laboratories, Inc. | Purification of immunoconjugates |
| JPWO2011001963A1 (ja) | 2009-07-03 | 2012-12-13 | 旭化成ケミカルズ株式会社 | 多孔質基材に固定されたグラフト鎖に結合しているアミノ基及びアルキル基を有する多孔膜を用いた抗体の精製方法 |
| ES2813398T3 (es) | 2009-10-20 | 2021-03-23 | Abbvie Inc | Aislamiento y purificación de anticuerpos anti-IL-13 usando cromatografía de afinidad a Proteína A |
| ES2982297T3 (es) | 2009-12-18 | 2024-10-15 | Novartis Ag | Método de cromatografía de afinidad |
| US20130116413A1 (en) * | 2009-12-29 | 2013-05-09 | Dr. Reddy's Laboratories, Inc. | Purification of proteins |
| WO2011088225A1 (en) | 2010-01-15 | 2011-07-21 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| RU2012146448A (ru) | 2010-04-14 | 2014-05-20 | Ф.Хоффманн-Ля Рош Аг | Удаление агрегатов иммуноглобулинов |
| GB201006753D0 (en) | 2010-04-22 | 2010-06-09 | Biotest Ag | Process for preparing an immunolobulin composition |
| US20110301333A1 (en) * | 2010-06-08 | 2011-12-08 | Millipore Corporation | Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts |
| US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
| AU2011325341B2 (en) | 2010-11-01 | 2015-12-17 | Dpx Holdings B.V. | Single unit ion exchange chromatography antibody purification |
| EP2670704B1 (en) | 2011-02-02 | 2018-12-12 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
| SG195306A1 (en) * | 2011-06-08 | 2013-12-30 | Agency Science Tech & Res | Purification of biological products by constrained cohydration chromatography |
| US20140187749A1 (en) * | 2011-06-16 | 2014-07-03 | Dsm Ip Assets B.V. | Single unit chromatography antibody purification |
| SG10201701224UA (en) | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
| US9815695B2 (en) | 2012-05-30 | 2017-11-14 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
| WO2014003142A1 (ja) * | 2012-06-27 | 2014-01-03 | 旭化成メディカル株式会社 | 抗体 |
| JP2014105180A (ja) * | 2012-11-27 | 2014-06-09 | Hoya Corp | モノマー化方法 |
| EP2968468B1 (en) | 2013-03-13 | 2021-07-14 | Buzzard Pharmaceuticals AB | Chimeric cytokine formulations for ocular delivery |
| HK1222871A1 (zh) * | 2013-05-13 | 2017-07-14 | Medimmune, Llc | 具有最小单体分离的重组多克隆抗体多聚体的分离 |
| EP3016729B1 (fr) | 2013-07-05 | 2020-03-25 | Laboratoire Francais du Fractionnement et des Biotechnologies Societe Anonyme | Matrice de chromatographie d'affinité |
| WO2015005960A1 (en) | 2013-07-12 | 2015-01-15 | Emd Millipore Corporation | Removal of fragments from a sample containing a target protein using activated carbon |
| JP6479305B2 (ja) * | 2013-07-12 | 2019-03-06 | 株式会社Ｕｍｎファーマ | ウイルス様粒子の精製方法 |
| JP6326499B2 (ja) | 2013-12-12 | 2018-05-16 | イー・エム・デイー・ミリポア・コーポレイシヨン | アクリルアミド含有フィルターを使用したタンパク質分離 |
| MX2016008498A (es) * | 2013-12-27 | 2016-10-07 | Chugai Pharmaceutical Co Ltd | Metodo para purificar anticuerpo que tiene bajo punto isoelectrico. |
| CN105407917B (zh) | 2014-06-23 | 2021-07-06 | 生物辐射实验室股份有限公司 | 磷灰石原位恢复 |
| US9802822B2 (en) * | 2014-06-23 | 2017-10-31 | Bio-Rad Laboratories, Inc. | Apatite pretreatment |
| JP6845139B2 (ja) | 2014-12-15 | 2021-03-17 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ | 粗製溶液からの標的分子捕捉 |
| WO2016149088A1 (en) * | 2015-03-13 | 2016-09-22 | Bristol-Myers Squibb Company | Use of alkaline washes during chromatography to remove impurities |
| US11661438B2 (en) | 2015-12-21 | 2023-05-30 | Pfizer, Inc. | Purification of antibody drug conjugates using a sodium phosphate gradient |
| US11186858B1 (en) | 2016-03-15 | 2021-11-30 | Fresenius Kabi Deutschland Gmbh | Methods for increasing biosimilarity |
| US11919924B1 (en) * | 2016-03-15 | 2024-03-05 | Fresenius Kabi Deutschland Gmbh | Methods of purifying and producing an adalimumab antibody |
| CN106977591B (zh) * | 2017-05-04 | 2020-05-05 | 广州康盛生物科技股份有限公司 | 一种分离纯化重组葡萄球菌蛋白a的方法 |
| MA49962A (fr) | 2017-08-22 | 2020-07-01 | Biogen Ma Inc | Procédés de purification d'anticorps présentant des agrégats réduits à poids moléculaire élevé |
| WO2019116096A1 (en) * | 2017-12-15 | 2019-06-20 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Production of fc fragments |
| TW202430218A (zh) | 2018-02-27 | 2024-08-01 | 美商輝瑞大藥廠 | 抗體純化 |
| WO2019183334A1 (en) | 2018-03-21 | 2019-09-26 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbents, devices and methods |
| JP2021519339A (ja) | 2018-04-03 | 2021-08-10 | メルク パテント ゲーエムベーハー | Ｃｅｘクロマトグラフィー媒体及び生物製剤供給原料からの標的タンパク質の低塩溶出 |
| JP6951051B2 (ja) * | 2018-05-24 | 2021-10-20 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 吸着剤の生産方法 |
| JP6704074B2 (ja) * | 2019-02-04 | 2020-06-03 | 株式会社Ｕｍｎファーマ | ウイルス様粒子の精製方法 |
| EP4061825A1 (en) | 2019-11-22 | 2022-09-28 | MorphoSys AG | Method to increase antibody yield during ion exchange chromatography |
| JP2020099909A (ja) * | 2020-03-17 | 2020-07-02 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 処理方法、生産方法およびハイドロキシアパタイト充填剤 |
| CN115397868A (zh) | 2020-03-30 | 2022-11-25 | 埃博灵克斯股份有限公司 | 用于产生和纯化多价免疫球蛋白单可变域的方法 |
| AU2021262609A1 (en) | 2020-05-01 | 2022-12-22 | Kashiv Biosciences, Llc | An improved process of purification of protein |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US523335A (en) | 1894-07-24 | Air-bolt for flour-mills | ||
| US419964A (en) | 1890-01-21 | Transom-lifter | ||
| US428894A (en) | 1890-05-27 | Process of making tool-handles | ||
| US514018A (en) | 1894-02-06 | Edward j | ||
| US4745183A (en) * | 1984-02-21 | 1988-05-17 | Bio-Rad Laboratories, Inc. | Use of hydroxylapatite for the analysis and preparation of purified monoclonal antibodies |
| JPH03291300A (ja) * | 1989-12-28 | 1991-12-20 | Mitsui Toatsu Chem Inc | ポリクローナル抗体の分離方法 |
| GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| PE20020574A1 (es) * | 2000-12-06 | 2002-07-02 | Wyeth Corp | Anticuerpos humanizados que reconocen el peptido amiloideo beta |
| ES2322945T5 (es) * | 2001-12-21 | 2012-10-17 | Immunex Corporation | Métodos para purficación de proteína |
| CA2516518A1 (en) | 2003-02-28 | 2004-09-10 | Lonza Biologics Plc. | Antibody purification by protein a and ion exchange chromatography |
| KR101221862B1 (ko) * | 2003-10-27 | 2013-01-14 | 와이어쓰 엘엘씨 | 하이드록시아파타이트 크로마토그래피를 사용한 고분자량응집체의 제거 |
-
2004
- 2004-10-06 KR KR1020067010375A patent/KR101221862B1/ko not_active Expired - Lifetime
- 2004-10-06 ES ES04794288T patent/ES2418830T3/es not_active Expired - Lifetime
- 2004-10-06 AU AU2004286938A patent/AU2004286938B2/en not_active Expired
- 2004-10-06 DK DK10174366.4T patent/DK2336172T3/en active
- 2004-10-06 NZ NZ547315A patent/NZ547315A/en unknown
- 2004-10-06 EP EP10174366.4A patent/EP2336172B1/en not_active Expired - Lifetime
- 2004-10-06 PL PL04794288T patent/PL1678208T3/pl unknown
- 2004-10-06 PL PL10174366T patent/PL2336172T3/pl unknown
- 2004-10-06 US US10/958,595 patent/US9469672B2/en active Active
- 2004-10-06 PT PT47942883T patent/PT1678208E/pt unknown
- 2004-10-06 JP JP2006538022A patent/JP5004586B2/ja not_active Expired - Lifetime
- 2004-10-06 DK DK04794288.3T patent/DK1678208T3/da active
- 2004-10-06 WO PCT/US2004/032883 patent/WO2005044856A2/en not_active Ceased
- 2004-10-06 BR BRPI0415887-3A patent/BRPI0415887B1/pt not_active IP Right Cessation
- 2004-10-06 CA CA2543193A patent/CA2543193C/en not_active Expired - Fee Related
- 2004-10-06 BR BR122020007658-5A patent/BR122020007658B1/pt active IP Right Grant
- 2004-10-06 RU RU2006113695/10A patent/RU2409591C2/ru active
- 2004-10-06 KR KR1020117030940A patent/KR101321876B1/ko not_active Expired - Lifetime
- 2004-10-06 CN CN201110221442.5A patent/CN102276717B/zh not_active Expired - Lifetime
- 2004-10-06 ES ES10174366T patent/ES2530446T3/es not_active Expired - Lifetime
- 2004-10-06 MX MXPA06004472A patent/MXPA06004472A/es active IP Right Grant
- 2004-10-06 PT PT101743664T patent/PT2336172E/pt unknown
- 2004-10-06 EP EP04794288.3A patent/EP1678208B1/en not_active Expired - Lifetime
- 2004-10-06 CN CN2004800387536A patent/CN1898266B/zh not_active Expired - Lifetime
-
2006
- 2006-04-26 IL IL175229A patent/IL175229A/en active IP Right Grant
- 2006-05-19 CO CO06048228A patent/CO5690649A2/es not_active Application Discontinuation
- 2006-05-25 EC EC2006006583A patent/ECSP066583A/es unknown
-
2010
- 2010-09-24 RU RU2010139827/10A patent/RU2573910C2/ru active
-
2012
- 2012-01-26 JP JP2012013648A patent/JP5490156B2/ja not_active Expired - Lifetime
-
2013
- 2013-03-14 US US13/803,071 patent/US9822143B2/en not_active Expired - Lifetime
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2007532477A5 (https=) | ||
| KR101569783B1 (ko) | 항체의 정제 방법 | |
| JP2017086090A5 (https=) | ||
| JP3676817B2 (ja) | 新規因子ｉｘ精製法 | |
| EP2238154B1 (en) | Enhanced purification of phosphorylated and non-phosphorylated biomolecules by apatite chromatography | |
| JP2020040952A5 (https=) | ||
| RU2006113695A (ru) | Удаление агрегатов с высокой молекулярной массой путем хроматографии на гидроксиапатитах | |
| JP2015503524A5 (https=) | ||
| Belew et al. | High-performance analytical applications of immobilized metal ion affinity chromatography | |
| RU2011112989A (ru) | Способы очистки однодоменных антигенсвязывающих молекул | |
| US20110166332A1 (en) | Enhanced antibody aggregate removal with capto adhere in the presence of protein-excluded zwitterions | |
| JP2023537565A5 (https=) | ||
| RU2014130017A (ru) | Способы повышения эффективности этапов очистки белка, находящихся ниже по потоку, с использованием мембранной ионообменной хроматографии | |
| CN116261483B (zh) | 多模式金属亲和加工衣壳 | |
| JP2003096092A (ja) | 陽イオン交換クロマトグラフィーを介する薬理学的に活性なタンパク質の精製方法 | |
| CN102234332B (zh) | 一种重组人血白蛋白及其融合蛋白的分离纯化工艺 | |
| CA2560012A1 (en) | A method for chromatographic purification | |
| JP2014507368A5 (https=) | ||
| CN104507954A (zh) | 磨光白蛋白的方法 | |
| Lu et al. | Evaluation on adsorption selectivity of immunoglobulin G with 2-mercapto-1-methyl-imidazole-based hydrophobic charge-induction resins | |
| CA2404155A1 (en) | Methods for purifying highly anionic proteins | |
| Pros et al. | Ion exchange chromatography | |
| CN101119797A (zh) | 液相色谱法 | |
| Ramadan et al. | Separation of serum proteins on a Fe3+-monohydroxamate adsorbent | |
| Sharma et al. | Adsorption equilibrium and kinetics of egg-white proteins on immobilized metal ion affinity gels for designing fractionation |